Abstract:
:Properly metabolized globin synthesis and iron uptake are indispensable for erythroid cell differentiation and maturation. Mitochondrial participation is crucial in the process of haeme synthesis for cytochromes and haemoglobin. We studied the final biosynthesis site of haemoglobin using an ultrastructural approach, with erythroid cells obtained from rabbit embryos, in order to compare these results with those of animals treated with saponine or phenylhydrazine. Our results are similar to those obtained in assays with adult mammals, birds, amphibians, reptiles and fish, after induction of haemolytic anaemia. Therefore, the treatment did not interfere with the process studied, confirming our previous findings. Immunoelectron microscopy showed no labelling of mitochondria or other cellular organelles supposedly involved in the final biosynthesis of haemoglobin molecules, suggesting instead that it occurs free in the cytoplasm immediately after the liberation of haeme from the mitochondria, by electrostatic attraction between haeme and globin chains.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Cianciarullo AM,Bertho AL,Soares MJ,Hosoda TM,Nogueira-Silva S,Beçak Wdoi
10.1016/s1065-6995(03)00157-4keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
747-53issue
9eissn
1065-6995issn
1095-8355pii
S1065699503001574journal_volume
27pub_type
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