Up-regulation of ectonucleotidase activity after cortical stab injury in rats.

Abstract:

:The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 5'-nucleotidase were assessed by measuring the levels of ATP, ADP and AMP hydrolysis in the crude membrane preparations obtained from injured left cortex, right cortex, left and right caudate nucleus, whole hippocampus and cerebellum. Significant increase in NTPDase and ecto 5'-nucleotidase activities was observed in the injured cortex following CSI, whereas in other brain areas only an increase in ecto 5'-nucleotidase activity was seen. Immunohistochemical analysis performed using antibodies specific to NTPDase 1 and ecto 5'-nucleotidase demonstrated that CSI induced significant changes in enzyme expression around the injury site. Immunoreactivity patterns obtained for NTPDase 1 and ecto 5'-nucleotidase were compared with those obtained for glial fibrillary acidic protein, as a marker of astrocytes and complement receptor type 3 (OX42), as a marker of microglia. Results suggest that up-regulation of ectonucleotidase after CSI is catalyzed by cells that activate in response to injury, i.e. cells immunopositive for NTPDase 1 were predominantly microglial cells, whereas cells immunopositive for ecto 5'-nucleotidase were predominantly astrocytes.

journal_name

Cell Biol Int

authors

Nedeljkovic N,Bjelobaba I,Subasic S,Lavrnja I,Pekovic S,Stojkov D,Vjestica A,Rakic L,Stojiljkovic M

doi

10.1016/j.cellbi.2006.03.001

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

541-6

issue

6

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(06)00064-3

journal_volume

30

pub_type

杂志文章
  • Characterization of MSCs from human placental decidua basalis in hypoxia and serum deprivation.

    abstract::Recently, we reported that human PDB (placental decidua basalis) is an excellent source of MSCs (mesenchymal stem cells), meanwhile, PDB-MSCs could survive under hypoxia and serum deprivation. Herein, we investigated the proliferation, clonogentic efficiency, phenotypes, metabolic activity and cytokines secretion of P...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090044

    authors: Huang YC,Yang ZM,Jiang NG,Chen XH,Li XQ,Tan MY,Zhou KP,Tang L,Xie HQ,Deng L

    更新日期:2010-01-27 00:00:00

  • Tyrosine phosphorylation is involved in Ca(2+)entry in human gingival fibroblasts.

    abstract::Bradykinin (1 microM) and histamine (100 microM) evoked an initial transient increase and a subsequent sustained increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded human gingival fibroblasts, which may be attributed to Ca(2+) release from intracellular stores and Ca(2+) entry from extracellu...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/s1065-6995(03)00125-2

    authors: Ogata Y,Nakao S,Shimizu E,Matsuda-Honjyo Y,Yamazaki M,Furuyama S,Sugiya H

    更新日期:2003-01-01 00:00:00

  • Apoptotic effect of eugenol in human colon cancer cell lines.

    abstract::Eugenol, a natural compound available in honey and various plants extracts including cloves and Magnoliae flos, is exploited for various medicinal applications. Since most of the drugs used in the cancer are apoptotic inducers, the apoptotic effect and anticancer mechanism of eugenol were investigated against colon ca...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100118

    authors: Jaganathan SK,Mazumdar A,Mondhe D,Mandal M

    更新日期:2011-06-01 00:00:00

  • Invariant mRNA and mitotic protein breakdown solves the Russian Doll problem of the cell cycle.

    abstract::It has been proposed that cyclical gene expression occurs at a large number of different times during the cell cycle. The existence of a large number of cycle-specific variations in mRNA and protein during the eukaryotic cell cycle raises the problem of how cell-cycle variations are regulated. This is the "infinite re...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.10.004

    authors: Cooper S,Shedden K,Vu-Phan D

    更新日期:2009-01-01 00:00:00

  • Aplasia Ras homologue member I overexpression induces apoptosis through inhibition of survival pathways in human hepatocellular carcinoma cells in culture and in xenograft.

    abstract::The aim of the present study was to determine the effects of ARHI (aplasia Ras homologue member I; also known as DIRAS3), a member of the Ras superfamily, on HCC (hepatocellular carcinoma) cells and to define the molecular pathways involved. Stable transfection of ARHI into the HCC cell line Hep3B that lacks expressio...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110023

    authors: Pei XH,Yang Z,Liu HX,Qiao SS

    更新日期:2011-10-01 00:00:00

  • Expression and immunohistochemical localization of galectin-3 in various mouse tissues.

    abstract::The expression and immunohistochemical localization of galectin-3, a beta-galactoside-binding protein, was studied in several mouse tissues. Galectin-3 expression was low in the cerebrum, heart, and pancreas, and moderate in the liver, ileum, kidney, and adrenal gland. High expression of galectin-3 was found in the lu...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.11.036

    authors: Kim H,Lee J,Hyun JW,Park JW,Joo HG,Shin T

    更新日期:2007-07-01 00:00:00

  • In vitro culture and induced differentiation of sheep skeletal muscle satellite cells.

    abstract::Skeletal muscle satellite cells are adult muscle-derived stem cells receiving increasing attention. Sheep satellite cells have a greater similarity to human satellite cells with regard to metabolism, life span, proliferation and differentiation, than satellite cells of the rat and mouse. We have used 2-step enzymatic ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110487

    authors: Wu H,Ren Y,Li S,Wang W,Yuan J,Guo X,Liu D,Cang M

    更新日期:2012-06-01 00:00:00

  • The effect of oxidative stress induced by t-butyl hydroperoxide on the structural dynamics of membrane proteins of Chinese hamster fibroblasts.

    abstract::A method based on the measurement at room temperature of tryptophan phosphorescence (RTTP) gives the unique possibility to investigate the dynamic structure of membrane proteins without their isolation from cells. This method was used to study the influence of tert- butyl hydroperoxide (t -BHP) on Chinese hamster fibr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0366

    authors: Mazhul V,Shcherbin D,Zavodnik I,Rekawiecka K,Bryszewska M

    更新日期:1999-01-01 00:00:00

  • Nitric oxide induced oxidative changes in erythrocyte membrane components.

    abstract::The effects of NO in its environment may vary considerably depending on various factors. This study shows oxidative mechanism of cellular membrane alterations, which is not associated with triggering of ONOOH generation but is induced by pure NO. Our investigation examined the influence of low concentration of NO (0.1...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.08.020

    authors: Brzeszczynska J,Gwozdzinski K

    更新日期:2008-01-01 00:00:00

  • Unique membrane-interacting properties of the immunostimulatory cationic peptide KLKL(5)KLK (KLK).

    abstract::We have monitored the effects of KLKL(5)KLK (KLK), a derivative of a natural cationic antimicrobial peptide (CAP) on isolated membrane vesicles, and investigated the partition of the peptide within these structures. KLK readily interacted with fluorescent dyes entrapped in the vesicles without apparent pore formation....

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.08.024

    authors: Aichinger MC,Ortbauer M,Reipert S,Zauner W,Bogner P,Froschauer E,Nowikovsky K,Lingnau K,von Gabain A,Schweyen R,Henics T

    更新日期:2008-11-01 00:00:00

  • Effect of magnesium on calcium-induced depolarisation of mitochondrial transmembrane potential.

    abstract::An effect of magnesium on calcium-induced depolarisation of mitochondrial transmembrane potential (DeltaPsi(m)) was investigated. Depending on the presence of Mg(2+), addition of Ca(2+) to suspension of isolated rat heart mitochondria induced either reversible depolarisation or irreversible collapse of succinate-drive...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.08.024

    authors: Racay P

    更新日期:2008-01-01 00:00:00

  • YY1-induced upregulation of FOXP4-AS1 and FOXP4 promote the proliferation of esophageal squamous cell carcinoma cells.

    abstract::Esophageal squamous cell carcinoma (ESCC) belongs to one of the most common malignant tumors worldwide and possesses high mortality. Long non-coding RNAs (lncRNAs) have been demonstrated to be essential biological participants in the progression of ESCC. On the basis of bio-informatics prediction, forkhead box P4 anti...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11338

    authors: Li Y,Li T,Yang Y,Kang W,Dong S,Cheng S

    更新日期:2020-07-01 00:00:00

  • Discrepant effects of mammalian factors on molluscan cell motility, chemotaxis and phagocytosis: divergent evolution or finely tuned contingency?

    abstract::Cell motility, cell migration and phagocytosis are distinct, though frequently sequential, processes. They are fundamental for the maintenance of homoeostasis in single cells as well as in pluricellular organisms. Like vertebrates, invertebrate immune functions are strictly dependent on cell motility, chemotaxis and p...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1042/CBI20100514

    authors: Malagoli D,Ottaviani E

    更新日期:2010-11-01 00:00:00

  • Gelatinases and metalloproteinase inhibitor secreted by murine colonic carcinoma cells with differing metastatic potential.

    abstract::Gelatinase activity and inhibitory activity against collagenase were measured in serum-free medium conditioned by murine colonic carcinoma cells with different spontaneous metastatic potentials to the lung. The medium conditioned with poorly metastatic NM11 cells gave higher inhibitory activity than that conditioned w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1994.1057

    authors: Kishi J,Tanaka R,Koiwai O,Yamagata S,Numata Y,Hayakawa T,Shimizu S

    更新日期:1994-03-01 00:00:00

  • Differentiation potential of menstrual blood- versus bone marrow-stem cells into glial-like cells.

    abstract::Menstrual blood is easily accessible, renewable, and inexpensive source of stem cells that have been interested for cell therapy of neurodegenerative diseases. In this study, we showed conversion of menstrual blood stem cells (MenSCs) into clonogenic neurosphere- like cells (NSCs), which can be differentiated into gli...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10245

    authors: Azedi F,Kazemnejad S,Zarnani AH,Behzadi G,Vasei M,Khanmohammadi M,Khanjani S,Edalatkhah H,Lakpour N

    更新日期:2014-05-01 00:00:00

  • An appreciation of the prescience of Don Gilbert (1930-2011): master of the theory and experimental unravelling of biochemical and cellular oscillatory dynamics.

    abstract::We review Don Gilbert's pioneering seminal contributions that both detailed the mathematical principles and the experimental demonstration of several of the key dynamic characteristics of life. Long before it became evident to the wider biochemical community, Gilbert proposed that cellular growth and replication neces...

    journal_title:Cell biology international

    pub_type: 传,历史文章,杂志文章

    doi:10.1002/cbin.11341

    authors: Gilbert K,Hammond KD,Brodsky VY,Lloyd D

    更新日期:2020-06-01 00:00:00

  • Vasopressin stimulates tyrosine phosphorylation by activation of PKC in the rat smooth muscle cell line, A-10.

    abstract::Arginine vasopressin (AVP)-induced tyrosine phosphorylation was studied in a rat smooth muscle cell line, A-10, by western blotting, using a monoclonal antibody against phosphotyrosine. AVP stimulated the phosphorylation of several cellular proteins of molecular mass 60-130 kDa in a time- and dose-dependent manner. Ph...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0343

    authors: Gonzalez CB,Reyes CE,Figueroa CD,Barra V,Troncoso S

    更新日期:1999-01-01 00:00:00

  • Changes in VEGF expression and DNA synthesis in hepatocytes from hepatectomized and tumour-bearing mice.

    abstract::Transplanted tumours could modify the intensity and temporal distribution of the cellular proliferation in normal cell populations, and partial hepatectomy alters the serum concentrations of substances involved in cellular proliferation, leading to the compensatory liver hyperplasia. The following experiments were des...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090282

    authors: García MN,Andrini LB,Inda AM,Ronderos JR,Hijano JC,Errecalde AL

    更新日期:2010-02-05 00:00:00

  • Thiazolidinedione induces the adipose differentiation of fibroblast-like cells resident within bovine skeletal muscle.

    abstract::To investigate the role of peroxisome proliferator-activated receptor gamma (PPARgamma) in adipocyte formation within the skeletal muscle of beef cattle, fibroblast-like cells were isolated from the longissimus muscle of cattle and cultured with activators of murine PPARgammaA thiazolidinedione T-174, which is a speci...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1998.0270

    authors: Torii SI,Kawada T,Matsuda K,Matsui T,Ishihara T,Yano H

    更新日期:1998-01-01 00:00:00

  • E-cadherin-mediated contact of endothelial progenitor cells with mesenchymal stem cells through β-catenin signaling.

    abstract::Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) are attached to each other in the bone marrow (BM) cavity and in in vitro cultures, and this adhesion has important physiological significance. We demonstrated that cell proliferation could be promoted when MSCs were co-cultured with EPCs, which was...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10579

    authors: Xia J,Zhang H,Gao X,Guo J,Hou J,Wang X,Wang S,Yang T,Zhang X,Ge Q,Wan L,Cheng W,Zheng J,Chen X,Wu X

    更新日期:2016-04-01 00:00:00

  • Extended chromatin fibres: crystallinity, molecular order and reactivity to concanavalin-A.

    abstract::The present study was undertaken to test the reproducible formation of the extended chromatin fibres (ECF), beads and superbeads, and detect molecular order and crystallinity by optical anisotropies in those structures. Chicken erythrocyte smears and mouse liver cell imprints were treated with 2.0-3.0 m NaCl solution ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0555

    authors: de Campos Vidal B

    更新日期:2000-01-01 00:00:00

  • Cholesterol esterification in human monocyte-derived macrophages is inhibited by protein kinase C with dual roles for mitogen activated protein kinases.

    abstract::The possible role of protein kinase C (PKC) and mitogen activated protein (MAP) kinases in the stimulation of cholesterol esterification by acetylated low density lipoprotein (acLDL) in human monocyte-derived macrophages (HMDM) was studied. Cholesterol esterification, as assessed by the rate of incorporation of [3H]-o...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.07.007

    authors: Napolitano M,Avella M,Goode NT,Botham KM,Bravo E

    更新日期:2004-01-01 00:00:00

  • Mitotic catastrophe and endomitosis in tumour cells: an evolutionary key to a molecular solution.

    abstract::Following genotoxic insult, p53 mutated tumour cells undergo mitotic catastrophe. This is characterised by a switch from mitosis to the endocycle. The essential difference between mitosis and the endocycle is that in the latter, DNA synthesis is uncoupled from cell division, which leads to the formation of endopolyplo...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellbi.2005.10.005

    authors: Erenpreisa J,Kalejs M,Cragg MS

    更新日期:2005-12-01 00:00:00

  • CFTR inhibits the invasion and growth of esophageal cancer cells by inhibiting the expression of NF-κB.

    abstract::Our study aimed to explore the function of cystic fibrosis transmembrane conductance regulator (CFTR) in esophageal cancer. Twenty patients with esophageal squamous cell carcinoma (ESCC) and 20 patients with esophageal adenocarcinoma (EA) were enrolled in this study. The levels of CFTR and NF-κB in tumor tissues and a...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11069

    authors: Li W,Wang C,Peng X,Zhang H,Huang H,Liu H

    更新日期:2018-12-01 00:00:00

  • Retinal and olfactory bulb precursor cells show distinct responses to FGF-2 and laminin.

    abstract::We analyzed whether the embryonic (E12.5-E14.5) mouse retina possesses genuine neural stem cells and how they respond to defined growth factors and extracellular matrix molecules. Whereas most combinations produced no or limited cell survival and proliferation in culture, FGF-2 plus heparin and laminin stimulated prol...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.11.031

    authors: Otaegi G,de Pablo F,Vicario-Abejón C,de la Rosa EJ

    更新日期:2007-07-01 00:00:00

  • δ-Catenin interacts with LEF-1 and negatively regulates its transcriptional activity.

    abstract::δ-Catenin and β-catenin belong to different subfamilies of armadillo proteins but share some common binding partners, such as E-cadherin. This is the first study that demonstrated a novel common binding partner for δ-catenin and β-catenin, lymphoid enhancer factor-1 (LEF-1). We found that the N-terminus of δ-catenin (...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10465

    authors: He Y,Ki H,Kim H,Kim K

    更新日期:2015-08-01 00:00:00

  • Retention and transmission of active transcription memory from progenitor to progeny cells via ligand-modulated transcription factors: elucidation of a concept by BIOPIT model.

    abstract::Observations made in live cells have clearly demonstrated that agonist-activated steroid/nuclear receptors reorganize in the nucleoplasm into hundreds of discrete speckled structures commonly referred to as nuclear foci. Subsequent studies have shown that nuclear foci are formed only with agonist- and not with pure an...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090329

    authors: Kumar S,Saradhi M,Chaturvedi NK,Tyagi RK

    更新日期:2012-02-01 00:00:00

  • gp130-specific antisense oligonucleotides inhibit IL-6 signal inducing junB mRNA transcription in the human hepatoma cell line, HepG2.

    abstract::The biosynthesis of interleukin-6 receptor (IL-6R) and gp130 in vitro was blocked using specific antisense oligonucleotides (ASO) in HepG2 liver cells and the efficacy of various ASOs was tested on the generation of IL-6-induced junB mRNA. We used three ASOs specific for the IL-6 receptor, three specific for gp130 and...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0765

    authors: Varga VL,Fülöp AK,Holub MC,Tóth S,Szalai C,Falus A

    更新日期:2001-01-01 00:00:00

  • Human insulin receptor juxtamembrane domain independent insulin signaling.

    abstract::The exon 16-encoded juxtamembrane (JM) domain of human insulin receptor (hIR) harbors the NPEY motif which couples the insulin-activated hIR kinase to downstream signal transduction molecules. We sought to determine if signal transduction requires the entire exon 16-encoded 22-amino acid JM domain. Transfected CHO cel...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.01.033

    authors: Sattar AA,Berhanu C,Gebreselassie S,Berhanu P

    更新日期:2007-08-01 00:00:00

  • Expression of the hormonal FGF co-receptor Klotho beta in the Xenopus laevis model.

    abstract::Klotho beta (Klb), a single-pass transmembrane protein, has been described as a co-receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this pa...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11059

    authors: Chen G,Tao Q

    更新日期:2019-02-01 00:00:00