Abstract：
:We analyzed whether the embryonic (E12.5-E14.5) mouse retina possesses genuine neural stem cells and how they respond to defined growth factors and extracellular matrix molecules. Whereas most combinations produced no or limited cell survival and proliferation in culture, FGF-2 plus heparin and laminin stimulated proliferation and the formation of aggregates composed, after two days, of 95.2% nestin-positive cells. However, cells in these aggregates could only be passaged poorly, lost nestin expression and proliferative capacity, and differentiated into neurons. Under the same conditions, olfactory bulb precursor cells divided efficiently and could be expanded. These data suggest that, in addition to FGF-2 and laminin, embryonic retinal neuroepithelial cells need additional extrinsic and/or intrinsic regulators to maintain cell proliferation and self-renewal.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Otaegi G,de Pablo F,Vicario-Abejón C,de la Rosa EJdoi
10.1016/j.cellbi.2006.11.031subject
Has Abstractpub_date
2007-07-01 00:00:00pages
752-8issue
7eissn
1065-6995issn
1095-8355pii
S1065-6995(06)00297-6journal_volume
31pub_type
杂志文章abstract：:Trophoblast cells play a vital role in embryo implantation. Exploring cytokines secreted by trophoblast cells will be significant in revealing the molecular mechanism of embryo implantation. In vitro-cultured trophoblast cells treated with Lefty revealed that Lefty could downregulate the expression of Nodal while prom...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10976
更新日期：2018-09-01 00:00:00
abstract：:Corticotropin-releasing hormone, which is the predominant regulator of neuroendocrine responses to stress, attenuates inflammation through stimulation of glucocorticoid release. Enhanced corticotropin-releasing hormone expression has been detected in inflammatory cells of the vascular endothelium, where it acts as a l...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11188
更新日期：2019-05-29 00:00:00
abstract：:Hypoxia is a condition in which the whole body or a region of the body is deprived of oxygen supply. The brain is very sensitive to the lack of oxygen and cerebral hypoxia can rapidly cause severe brain damage. Astrocytes are essential for the survival and function of neurons. Therefore, protecting astrocytes against ...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11335
更新日期：2020-06-01 00:00:00
abstract：:Genistein is one of the naturally occurring isoflavones present in plants such as soybeans and is commonly found in a variety of human foods. A number of studies indicated that this class of compounds exerts anticancerogenic and antimutagenic effects in various in vitro systems and in vivo animal models. We studied th...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2008.04.004
更新日期：2008-08-01 00:00:00
abstract：:EGFP (enhanced green fluorescent protein) tagged to either the N (amino)-terminus [EGFP/hERG (human ether-a-go-go-related gene)] or C (carboxyl)-terminus (hERG/EGFP) of hERG channel is used to study mutant channel protein trafficking for several years. However, it has been reported that the process can alter hERG chan...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1042/CBI20100022
更新日期：2011-03-01 00:00:00
abstract：:Peroxisomicine A1 (PA1) is a potential antineoplastic agent with high and selective toxicity toward peroxisomes of tumor cells. Pexophagy is a selective autophagy process that degrades damaged peroxisomes; this process has been studied mainly in methylotrophic yeasts. There are two main modes of pexophagy in yeast: ma...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11280
更新日期：2020-03-01 00:00:00
abstract：:The aim of this study was to determine the polarization of macrophages in the tumor microenvironment, as well as the effect of soluble factors secreted from these polarized macrophages on etoposide-induced cancer cell apoptosis. We investigated the effect of soluble factors secreted from the supernatant of PC3 cells t...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11410
更新日期：2020-10-01 00:00:00
abstract：:The induction and maintenance of immunologic tolerance at the feto-maternal interface is necessary for a successful pregnancy. The most accepted hypothesis for the mechanism underlying this tolerance is that pregnancy-induced foetal antigen-specific maternal T regulatory (T(reg) ) cells mediate maternal tolerance to t...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10023
更新日期：2013-02-01 00:00:00
abstract：:A plant transformation and selection system has been developed utilizing a modified tubulin gene as a selectable marker. The vector constructs carrying a mutant alpha-tubulin gene from goosegrass conferring resistance to dinitroaniline herbicides were created for transformation of monocotyledonous and dicotyledonous p...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.11.012
更新日期：2008-05-01 00:00:00
abstract：:Lead is a heavy metal of considerable environmental and occupational concern and there is growing evidence that it is toxic to the human immune system. In this regard, this study examined the effect of lead (Pb) exposure to peritoneal macrophages (Mvarphis) of mice (Mus musculus) cultivated in DMEM medium supplemented...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2006.03.010
更新日期：2006-07-01 00:00:00
abstract：:Life cannot be simply defined in biochemical terms but it is associated with autodynamic behaviour. This fact implies that all aspects of cell biology should be viewed in terms of the resultant temporal features. Theoretical arguments indicate that the dynamic state can be explained only by the existence of periodicit...
journal_title：Cell biology international
pub_type： 杂志文章,评审
doi：10.1006/cbir.2000.0571
更新日期：2000-01-01 00:00:00
abstract：:Although several species of Tetrahymena are often described as histophagous and opportunistic pathogens of fish, little is known about ciliate/fish cell interactions, but one approach for studying these is in vitro with cell lines. In this study, T. thermophila, B1975 (wild type) and NP1 (temperature sensitive mutant ...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10310
更新日期：2014-10-01 00:00:00
abstract：:The spatial distribution of acid membrane organelles and their relationships with normal and vacuolated transverse tubules has been studied in living frog skeletal muscle fibres using confocal microscopy. Acridine orange (AO) was used to evaluate acid compartments, while a lipophilic styryl dye, RH 414, was employed t...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2006.06.017
更新日期：2006-11-01 00:00:00
abstract：:BPD (bronchopulmonary dysplasia) is predominantly characterized by persistent abnormalities in lung structure and arrested lung development, but therapy can be palliative. While promising, the use of BMSC (bone marrow-derived mesenchymal stem cell) in the treatment of lung diseases remains controversial. We have asses...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1042/CBI20110447
更新日期：2012-06-01 00:00:00
abstract：:Mouse trisomy 16 (Ts16) appears to provide an animal model of Down's syndrome in that a portion of mouse chromosome 16 is syntenic with part of human chromosome 21. Trisomy 21 in human beings leads to the mental retardation of Down's syndrome and in middle age, to some presenile anatomic and clinical features of Alzhe...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1996.0089
更新日期：1996-10-01 00:00:00
abstract：:Tripartite motif-containing 22 (TRIM22) is reported to participate in numerous cellular activities. Recent studies confirm that TRIM22 is a target gene for P53, and inhibits clonogenic growth of leukemic U-937 cells. The current study aims to discover the effect of TRIM22 in progression of human chronic myeloid leukem...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10989
更新日期：2018-09-01 00:00:00
abstract：:Circulating endothelial progenitor cells (EPCs) play a key role in restoring endothelial function and enhancing angiogenesis. However, the effects of low-dose aspirin on circulating EPCs are not well known. We investigated the effects of low-dose aspirin on EPC migration, adhesion, senescence, proliferation, apoptosis...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2008.03.004
更新日期：2008-07-01 00:00:00
abstract：:Oral squamous cell carcinoma (OSCC) is a serious global health problem. Recently, accumulating microRNA (miRNA) has emerged as crucial players in the development and progression of carcinomas including OSCC. Our study aimed to further investigate the roles of miR-140-5p in OSCC tumorigenesis and related molecular basi...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11213
更新日期：2019-08-08 00:00:00
abstract：:Small heat shock proteins (shsps) are molecular chaperones that are inducible by environmental stress. In this study, immunocytochemical analysis and laser scanning confocal microscopy revealed that the shsp family, hsp30, was localized primarily in the cytoplasm of Xenopus A6 kidney epithelial cells after heat shock ...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2004.12.006
更新日期：2005-03-01 00:00:00
abstract：:Transplanted tumours could modify the intensity and temporal distribution of the cellular proliferation in normal cell populations, and partial hepatectomy alters the serum concentrations of substances involved in cellular proliferation, leading to the compensatory liver hyperplasia. The following experiments were des...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1042/CBI20090282
更新日期：2010-02-05 00:00:00
abstract：:Mitotic spindles of the diatom Pinnularia viritiformis stained with the fluorescent actin-labelling reagent bodipyphallacidin revealed actin among the chromosomes and extending along the spindle to the poles. This is the first report of actin's presence within spindles of this ecologically important group of organisms...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2004.04.010
更新日期：2004-01-01 00:00:00
abstract：:Steroid hormones and their receptors play pivotal roles in avian reproduction and development. 17β-estradiol (E2 ) is an essential mediator in ovarian development. In this study, Day 3 (D3) chicks were injected with E2 (0.025, 0.25 or 2.5 mg/kg body weight) for 4 days to assess the effects of estrogen on avian follicl...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10766
更新日期：2017-06-01 00:00:00
abstract：:An effect of magnesium on calcium-induced depolarisation of mitochondrial transmembrane potential (DeltaPsi(m)) was investigated. Depending on the presence of Mg(2+), addition of Ca(2+) to suspension of isolated rat heart mitochondria induced either reversible depolarisation or irreversible collapse of succinate-drive...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.08.024
更新日期：2008-01-01 00:00:00
abstract：:Lysosomal permeability to potassium ions is an important property of the organelle. Influence of the membrane physical state on the potassium ion permeability of isolated lysosomes was assessed by measuring the membrane potential with bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol and monitoring the lysosomal pro...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2004.12.002
更新日期：2005-06-01 00:00:00
abstract：:The cytokertatins in respiratory epithelial cells (REC) of human nasal polyps and turbinates were analyzed by immunohistochemistry. Cytokeratin 19 (CK19) was present in all REC, CK5 and 14 were expressed primarily in basal cells, and CK7, 8, and 18 were found in suprabasal cells. Differences in cytoplasmic locations w...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1995.1072
更新日期：1995-04-01 00:00:00
abstract：:At the end of transcardial perfusions with ice-cold physiological saline for 30 min or with isoosmotic potassium chloride for 5 min, but immediately before perfusion fixation, condenser-discharge electric shocks were administered to rats through surface electrodes pressed onto the temporal muscles of the scalped skull...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2005.11.002
更新日期：2006-02-01 00:00:00
abstract：:Photodynamic therapy (PDT) for cancer is a therapeutic modality in the treatment of tumors in which visible light is used to activate a photosensitizer. Cell membranes have been identified as an important intracellular target for singlet oxygen produced during the photochemical pathway. This study analyzed the cytotox...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2008.04.005
更新日期：2008-08-01 00:00:00
abstract：:This study provides evidence that, in the soluble fraction from buffer-washed pea thylakoids, one form of soluble carbonic anhydrase (CA) is associated with rubisco in a stromal protein complex. On native-PAGE gels, it is present as a protein band with MW approximately 160 kDa. On SDS-PAGE gels, it is resolved as a si...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2008.01.010
更新日期：2008-06-01 00:00:00
abstract：:A view is emerging that human amylin (HA) kills pancreatic islet beta-cells by apoptosis. This study strengthens this view by documenting time-dependent morphological and ultrastructural changes in 10 microm HA-treated cultured RINm5F islet beta-cells. Membrane blebbing and microvilli loss were the earliest detectable...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2000.0643
更新日期：2001-01-01 00:00:00
abstract：:Growing evidence has shown that apelin/APJ system functions as a critical mediator of cardiac development as well as cardiovascular function. Here, we investigated the role of apelin in the cardiomyogenic differentiation of mesenchymal stem cells derived from Wharton's jelly of human umbilical cord in vitro. In this r...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10581
更新日期：2016-05-01 00:00:00
