当前位置： SCI文献检索 > CELL BIOLOGY INTERNATIONAL期刊下所有文献 > 17-β estradiol attenuates the pro-oxidant activity of corticotropin-releasing hormone in macroendothelial cells.

17-β estradiol attenuates the pro-oxidant activity of corticotropin-releasing hormone in macroendothelial cells.

Abstract：

:Corticotropin-releasing hormone, which is the predominant regulator of neuroendocrine responses to stress, attenuates inflammation through stimulation of glucocorticoid release. Enhanced corticotropin-releasing hormone expression has been detected in inflammatory cells of the vascular endothelium, where it acts as a local regulator of endothelial redox homeostasis. Estrogens have beneficial effects on endothelial integrity and function, though the mechanism underlying their antioxidative effect remains as yet largely unknown. We therefore investigated the effect of 17β-estradiol on pro-oxidant action of corticotropin-releasing hormone in vitro in macroendothelial cells, and, more specifically, the role of 17β-estradiol on corticotropin-releasing hormone-induced activities/release of the antioxidant enzymes namely, endothelial nitric oxide synthase, superoxide dismutase, catalase, and glutathione. We observed that 17β-estradiol abolished the stimulatory effect of corticotropin-releasing hormone on intracellular reactive oxygen species levels and counteracted its inhibitory effect on endothelial nitric oxide synthase activity and nitric oxide release. In addition, 17β-estradiol significantly induced superoxide dismutase and catalase activity, an effect that was not significantly influenced by corticotropin-releasing hormone. Finally, 17β-estradiol significantly increased glutathione levels and the glutathione/glutathione + glutathione disulfide ratio, an action that was partially blocked by corticotropin-releasing hormone. Our results reveal that 17β-estradiol counterbalances corticotropin-releasing hormone-mediated pro-inflammatory action and thereby maintains the physiological threshold of the endothelial cell redox environment. These observations may be of importance, considering the protective role of estrogen in the development of atherosclerosis.

journal_name

Cell Biol Int

journal_title

Cell biology international

authors

Filiponi M,Gougoura SG,Befani C,Bargiota Α,Liakos P,Koukoulis GN

doi

10.1002/cbin.11188

subject

Has Abstract

pub_date

2019-05-29 00:00:00

eissn

1065-6995

issn

1095-8355

pub_type

杂志文章

相关文献

CELL BIOLOGY INTERNATIONAL文献大全
  • A novel estrogen receptor GPER mediates proliferation induced by 17β-estradiol and selective GPER agonist G-1 in estrogen receptor α (ERα)-negative ovarian cancer cells.

    abstract：:G protein-coupled estrogen receptor (GPER) is recently identified as a membrane-associated estrogen receptor that mediates non-genomic effects of estrogen. Our previous immunohistochemistry study found an association between GPER and the proliferation of epithelial ovarian cancer. However, the contributions and mechan...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10243

    authors： Liu H,Yan Y,Wen H,Jiang X,Cao X,Zhang G,Liu G

    更新日期：2014-05-01 00:00:00

  • Rapamycin improves insulin resistance and hepatic steatosis in type 2 diabetes rats through activation of autophagy.

    abstract：:Insulin resistance (IR) is a hallmark of type 2 diabetes mellitus (T2DM). This study aimed to explore the effects of rapamycin, a specific inhibitor of kinase mammalian target of rapamycin (mTOR), on IR in T2DM rats, and to validate whether the underlying mechanism was associated with autophagy. In this study, the mod...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11015

    authors： Zhou W,Ye S

    更新日期：2018-09-01 00:00:00

  • M2 macrophages induce EMT through the TGF-β/Smad2 signaling pathway.

    abstract：:IPF is characterized by fibroblast accumulation, collagen deposition, and ECM remodeling, with myofibroblasts believed to be the effector cell type. Myofibroblasts develop due to EMT of lung alveolar epithelial cells, which can be induced by TGF-β. M2 macrophages, a macrophage subpopulation, secrete large amounts of T...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10788

    authors： Zhu L,Fu X,Chen X,Han X,Dong P

    更新日期：2017-09-01 00:00:00

  • Extracellular calcium protects against verapamil-induced metaphase-II arrest and initiation of apoptosis in aged rat eggs.

    abstract：:Non-specific L-type calcium channel blockers, such as verapamil (> or =50 microM), induce metaphase-II (M-II) arrest and apoptosis in aged rat eggs cultured in Ca(2+)-deficient medium. However, the effects of extracellular Ca(2+) on verapamil-induced M-II arrest and apoptosis have not yet been reported. We have demons...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2009.01.001

    authors： Chaube SK,Tripathi A,Khatun S,Mishra SK,Prasad PV,Shrivastav TG

    更新日期：2009-03-01 00:00:00

  • Human insulin receptor juxtamembrane domain independent insulin signaling.

    abstract：:The exon 16-encoded juxtamembrane (JM) domain of human insulin receptor (hIR) harbors the NPEY motif which couples the insulin-activated hIR kinase to downstream signal transduction molecules. We sought to determine if signal transduction requires the entire exon 16-encoded 22-amino acid JM domain. Transfected CHO cel...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2007.01.033

    authors： Sattar AA,Berhanu C,Gebreselassie S,Berhanu P

    更新日期：2007-08-01 00:00:00

  • Single amino acid mutations in the Saccharomyces cerevisiae rhomboid peptidase, Pcp1p, alter mitochondrial morphology.

    abstract：:Key to mitochondrial activities is the maintenance of mitochondrial morphology, specifically cristae structures formed by the invagination of the inner membrane that are enriched in proteins of the electron transport chain. In Saccharomyces cerevisiae , these cristae folds are a result of the membrane fusion activitie...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11219

    authors： Huddleston ME,Xiao N,Both AP,Gordon DM

    更新日期：2019-08-23 00:00:00

  • Two-dimensional protein maps of xenopus eggs and embryos at different developmental stages.

    abstract：:Protein expression during the early development of Xenopus has been followed by 2D-polyacrylamide gel electrophoresis (PAGE). The analysis of two-dimensional maps of eggs and embryos at different stages of development has allowed the separation of more than 2000 spots. Identification of numerous polypeptides was obtai...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1998.0296

    authors： Tedeschi G,Bernardini G,Mortarino M,Negri A,Ceciliani F,Ronchi S

    更新日期：1998-01-01 00:00:00

  • Hypoxia-induced apoptosis of astrocytes is mediated by reduction of Dicer and activation of caspase-1.

    abstract：:Hypoxia is a condition in which the whole body or a region of the body is deprived of oxygen supply. The brain is very sensitive to the lack of oxygen and cerebral hypoxia can rapidly cause severe brain damage. Astrocytes are essential for the survival and function of neurons. Therefore, protecting astrocytes against ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11335

    authors： Kang YW,Kim YS,Park JY,Chu GE,Yang YC,Choi BY,Cho WG

    更新日期：2020-06-01 00:00:00

  • High resolution scanning force microscopy of cardiac myocytes.

    abstract：:The advent of scanning probe microscopy has introduced a powerful new method of probing the structural features of biological specimens. In this study, high resolution atomic force microscopy micrographs of single, isolated, cardiac myocytes are presented. Significantly, our images show not only the features to be exp...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2001.0813

    authors： Davis JJ,Hill HA,Powell T

    更新日期：2001-01-01 00:00:00

  • gp130-specific antisense oligonucleotides inhibit IL-6 signal inducing junB mRNA transcription in the human hepatoma cell line, HepG2.

    abstract：:The biosynthesis of interleukin-6 receptor (IL-6R) and gp130 in vitro was blocked using specific antisense oligonucleotides (ASO) in HepG2 liver cells and the efficacy of various ASOs was tested on the generation of IL-6-induced junB mRNA. We used three ASOs specific for the IL-6 receptor, three specific for gp130 and...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2001.0765

    authors： Varga VL,Fülöp AK,Holub MC,Tóth S,Szalai C,Falus A

    更新日期：2001-01-01 00:00:00

  • LncRNA-XIST promotes the oxidative stress-induced migration, invasion, and epithelial-to-mesenchymal transition of osteosarcoma cancer cells through miR-153-SNAI1 axis.

    abstract：:Osteosarcoma (OS) is the most common type of primary bone tumor that exhibits invasive growth and long-distance organ metastasis. Thus, investigating the specifically targeted therapeutic agents against metastatic osteosarcoma depends on understanding the molecular mechanisms. The long noncoding RNAs (lncRNA) XIST (X-...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11405

    authors： Wen JF,Jiang YQ,Li C,Dai XK,Wu T,Yin WZ

    更新日期：2020-10-01 00:00:00

  • Effects of extremely low concentrations of hormones on the insulin binding of Tetrahymena.

    abstract：:FITC-insulin binding to previously hormone-treated Tetrahymena was studied by flow cytometry and confocal microscopy. Hormones produced by Tetrahymena were chosen for study and the hormone concentrations were administered between 10(-6) and 10(-21)M for 30 min. Endorphin, serotonin and insulin significantly reduced th...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2006.06.019

    authors： Csaba G,Kovács P,Tóthfalusi L,Pállinger E

    更新日期：2006-12-01 00:00:00

  • Characterization of two populations of mesenchymal progenitor cells in umbilical cord blood.

    abstract：:Umbilical cord blood (UCB) is a valuable source for hematopoietic progenitor cell therapy. Moreover, it contains another subset of non-hematopoietic population referred to as mesenchymal progenitor cells (MPCs), which can be ex vivo expanded and differentiated into osteoblasts, chondrocytes and adipocytes. In this stu...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2005.12.009

    authors： Chang YJ,Tseng CP,Hsu LF,Hsieh TB,Hwang SM

    更新日期：2006-06-01 00:00:00

  • Long non-coding RNA MALAT1 functions as a mediator in cardioprotective effects of fentanyl in myocardial ischemia-reperfusion injury.

    abstract：:Long non-coding (lncRNA) MALAT1 can be increased by hypoxia or ischemic limbs. Also, downregulation of MALAT1 contributes to reduction of cardiomyocyte apoptosis. However, the functional involvement of MALAT1 in myocardial ischemia-reperfusion (I/R) injury has not been defined. This study investigated the functional i...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10701

    authors： Zhao ZH,Hao W,Meng QT,Du XB,Lei SQ,Xia ZY

    更新日期：2017-01-01 00:00:00

  • MicroRNA-195-5p suppresses osteosarcoma cell proliferation and invasion by suppressing naked cuticle homolog 1.

    abstract：:MiR-195-5p has been shown to play an essential role in human cancer progression. Nevertheless, the biological role of miR-195-5p in osteosarcoma development remains unclear. In this study, real-time PCR was performed to examine the miR-195-5p expression in human osteosarcoma cell lines. CCK-8 assay and Transwell assay...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10723

    authors： Qu Q,Chu X,Wang P

    更新日期：2017-03-01 00:00:00

  • Transcription factor XBP-1 is expressed during osteoblast differentiation and is transcriptionally regulated by parathyroid hormone (PTH).

    abstract：:X-box-binding protein 1 (XBP-1) is a basic-region leucine zipper protein in the cyclic AMP response element binding protein/activating transcription factor (CREB/ATF) family of transcription factors involved in different cell-differentiation processes. We have investigated the expression of XBP-1 in differentiating MC...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2005.03.018

    authors： Zambelli A,Mongiardini E,Villegas SN,Carri NG,Boot-Handford RP,Wallis GA

    更新日期：2005-08-01 00:00:00

  • Uptake of cadmium ions in white-rot fungus Trametes versicolor: effect of cd (II) ions on the activity of laccase.

    abstract：:The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2002.0887

    authors： Jarosz-Wilkolazka A,Malarczyk E,Pirszel J,Skowroński T,Leonowicz A

    更新日期：2002-01-01 00:00:00

  • Automated detection of morphological alterations during apoptosis.

    abstract：:A convenient means of measuring apoptosis is described using an automated analyzer (MF-1; Sysmex), which normally provides rapid measurement of malarial parasites. By means of this MF-1 procedure, apoptotic cells exhibited characteristic changes of light scatter (size) and fluorescence (DNA content) relating to cell s...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2000.0560

    authors： Lertworasirikul T,Bunyaratvej A,Kubota F

    更新日期：2000-01-01 00:00:00

  • Validation of common reference genes stability in exosomal mRNA-isolated from liver and breast cancer cell lines.

    abstract：:Accurate relative gene expression analysis by reverse transcription-quantitative polymerase chain reaction relies on the usage of suitable reference genes for data normalization. The RNA content of small extracellular vesicles including exosomes is growingly considered as cancer biomarkers. So, reliable relative quant...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11556

    authors： Gorji-Bahri G,Moradtabrizi N,Vakhshiteh F,Hashemi A

    更新日期：2021-01-27 00:00:00

  • Ectophosphatase activity in the triple-negative breast cancer cell line MDA-MB-231.

    abstract：:Breast cancer is one of the most common cancers in the female population worldwide, and its development is thought to be associated with genetic mutations that lead to uncontrolled and accelerated growth of breast cells. This abnormal behavior requires extra energy, and indeed, tumor cells display a rewired energy met...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11497

    authors： Lacerda-Abreu MA,Russo-Abrahão T,Leite Tenório Aguiar R,Monteiro RQ,Rumjanek FD,Meyer-Fernandes JR

    更新日期：2020-11-03 00:00:00

  • Bcl-2 down-regulation by small interfering RNA induces Beclin1-dependent autophagy in human SGC-7901 cells.

    abstract：:While Bcl-2 protein is involved in the regulation of apoptosis, recent research showed that Beclin1, described as the essential autophagy effector and haploinsufficient tumor suppressor, was originally isolated as a Bcl-2 interacting protein. Beclin1 interacts with Bcl-2 through a BH3 domain; nevertheless, the functio...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10333

    authors： Du Y,Ji X

    更新日期：2014-10-01 00:00:00

  • Invariant mRNA and mitotic protein breakdown solves the Russian Doll problem of the cell cycle.

    abstract：:It has been proposed that cyclical gene expression occurs at a large number of different times during the cell cycle. The existence of a large number of cycle-specific variations in mRNA and protein during the eukaryotic cell cycle raises the problem of how cell-cycle variations are regulated. This is the "infinite re...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2008.10.004

    authors： Cooper S,Shedden K,Vu-Phan D

    更新日期：2009-01-01 00:00:00

  • Propofol induces apoptosis of hepatocellular carcinoma cells by upregulation of microRNA-199a expression.

    abstract：:Propofol is one of the extensively commonly used intravenous anaesthetic agents. The effects of Propofol on hepatocellular carcinoma (HCC) growth inhibition and apoptosis have been examined. The techniques used were the MTT assay, flow cytometry, real-time PCR to assess miR-199a expression, as also caspase-8 and caspa...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10034

    authors： Zhang J,Wu GQ,Zhang Y,Feng ZY,Zhu SM

    更新日期：2013-03-01 00:00:00

  • Alphavbeta6 is required in maintaining the intestinal epithelial barrier function.

    abstract：:Epithelial barrier dysfunction is involved in a large number of diseases, but the pathogenesis is unclear. Integrin alphavbeta6 (avb6) in involved in the maintenance of the mucosal homeostasis. We have investigated the role of avb6 in maintaining the epithelial barrier function. Using T84 monolayers cultures, transepi...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10258

    authors： Yu Y,Chen S,Lu GF,Wu Y,Mo L,Liu ZQ,Zheng PY,Liu Z,Yang PC

    更新日期：2014-06-01 00:00:00

  • Effects of human yolk sac endothelial cells on supporting expansion of hematopoietic stem/progenitor cells from cord blood.

    abstract：:In order to investigate the effects of human yolk sac-derived endothelial cells (hYSECs) on the expansion of human hematopoietic stem/progenitor cells (HS/PCs) from umbilical cord blood (UCB) in vitro, we purified hYSEC-like cells from 4-5 week human yolk sacs, which were morphologically similar to endothelial cells a...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2006.06.006

    authors： Hu L,Cheng L,Wang J,Zhao H,Duan H,Lu G

    更新日期：2006-11-01 00:00:00

  • Bone marrow mesenchymal stem cells could acquire the phenotypes of epithelial cells and accelerate vaginal reconstruction combined with small intestinal submucosa.

    abstract：:Grafting material for vaginal reconstruction commonly includes the bowel, peritoneum, skin, and amniotic membrane. Bone marrow mesenchymal stem cells (MSCs) have the potential of multilineage differentiation into a variety of cells and have been widely explored in tissue engineering. In the current study, we examined ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10495

    authors： Li Y,Liu F,Zhang Z,Zhang M,Cao S,Li Y,Zhang L,Huang X,Xu Y

    更新日期：2015-11-01 00:00:00

  • Upregulated osterix expression elicited by Runx2 and Dlx5 is required for the accelerated osteoblast differentiation in PP2A Cα-knockdown cells.

    abstract：:Serine/threonine protein phosphatase 2A (PP2A) is involved in regulating various physiological processes including cell cycle, growth, apoptosis, and signal transduction. Osteoblast differentiation is controlled by main bone specific transcription factors including Osterix, distal-less homeobox 5 (Dlx5), and Runt-rela...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10902

    authors： Yang D,Okamura H,Qiu L

    更新日期：2018-04-01 00:00:00

  • Ultrastructural traits of apoptosis.

    abstract：:This review summarizes original and literature data on changes in the ultrastructure of major cell organelles during apoptosis obtained by transmission electron microscopy. Organelles that make the most crucial contribution to the initiation of apoptosis: plasma membrane, mitochondria, proteasomes, Golgi apparatus, an...

    journal_title：Cell biology international

    pub_type： 杂志文章,评审

    doi：10.1002/cbin.11148

    authors： Snigirevskaya ES,Komissarchik YY

    更新日期：2019-07-01 00:00:00

  • Bradykinin receptor-1 activation induces inflammation and increases the permeability of human brain microvascular endothelial cells.

    abstract：:Neuroinflammatory disorders such as Alzheimer's and Parkinson's diseases are characterised by chronic inflammation and loss of vascular integrity. Bradykinin 1 receptor (B1R) activation has been implicated in many neuroinflammatory diseases, but the contribution of B1R to inflammation and vascular breakdown is yet to ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11232

    authors： Mugisho OO,Robilliard LD,Nicholson LFB,Graham ES,O'Carroll SJ

    更新日期：2019-09-09 00:00:00

  • Neuroprotective effects of tetramethylpyrazine on hydrogen peroxide-induced apoptosis in PC12 cells.

    abstract：:In the present study, we investigated the effects of tetramethylpyrazine (TMP) on hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells. The apoptosis in H2O2-induced PC12 cells was accompanied by a decrease in Bcl-2/Bax protein ratio, release of cytochrome c to cytosol and the activation of caspase-3. TMP not only...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2006.10.001

    authors： Cheng XR,Zhang L,Hu JJ,Sun L,Du GH

    更新日期：2007-05-01 00:00:00