Characterization of two populations of mesenchymal progenitor cells in umbilical cord blood.

Abstract:

:Umbilical cord blood (UCB) is a valuable source for hematopoietic progenitor cell therapy. Moreover, it contains another subset of non-hematopoietic population referred to as mesenchymal progenitor cells (MPCs), which can be ex vivo expanded and differentiated into osteoblasts, chondrocytes and adipocytes. In this study, we successfully isolated the clonogenic MPCs from UCB by limiting dilution method. These cells exhibited two different morphologic phenotypes, including flattened fibroblasts (majority) and spindle-shaped fibroblasts (minority). Both types of MPCs shared similar cell surface markers except CD90 and had similar osteogenic and chondrogenic potentials. However, the spindle-shaped clones possessed the positive CD90 expression and showed a greater tendency in adipogenesis, while the flattened clones were CD90 negative cells and showed a lower tendency in adipogenesis. The high number of flattened MPCs might be linked to the less sensitivity of UCB-derived MPCs in adipogenic differentiation.

journal_name

Cell Biol Int

authors

Chang YJ,Tseng CP,Hsu LF,Hsieh TB,Hwang SM

doi

10.1016/j.cellbi.2005.12.009

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

495-9

issue

6

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(06)00018-7

journal_volume

30

pub_type

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