Abstract:
:It has been demonstrated that the crude extract of mosquito larvae alters the mitotic rate of several mouse cell populations of young growing mice (25 +/- 1 days old). Furthermore, the dialysed fraction of the extract inhibited proliferation of hepatocytes from hepatectomized adult male mice (90 days old). Sampling during the period between 16 and 24 h after treatment (when mitotic peak normally occurs) shows an inhibiting effect on the G1/S interphase, whereas evaluation during the dark phase of the circadian rhythm period (i.e. 4 to 12 h after treatment) shows an increment of the mitotic rates suggesting a probable effect at G2/M restriction point. In the present paper we report the effect of the mosquito larvae crude extract on the proliferative activity of tongue keratinocytes along a circadian rhythm period. Treatments were intraperitoneally applied at 16/00 Time of Day/Time post Injection and mice were killed at 00/08, 04/12, 08/16, 12/20 and 16/24 TD/TPI. As other cell populations previously analysed, the mitotic rate of tongue keratinocytes of extract receivers was significantly increased during night (when S phase normally occurs) and inhibited during the 08/16 to 16/24 period.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Ronderos JR,Riccillo FL,Ottino Adoi
10.1006/cbir.1996.0074subject
Has Abstractpub_date
1996-08-01 00:00:00pages
567-72issue
8eissn
1065-6995issn
1095-8355pii
S1065-6995(96)90074-8journal_volume
20pub_type
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