Abstract:
:We describe a method to hold living cells in place that ordinarily do not adhere to glass coverslips. The method, developed for insect spermatocytes but with application to other cell types, consists of embedding cells in a fibrin clot that forms after the enzyme thrombin cleaves the blood protein fibrinogen. The method permits continuous observation of living cells as they are treated with and recover from drug or other treatments: when held in the clot the living cells remain in place and keep their shapes when perfused with drugs that ordinarily cause drastic shape changes, and they remain in place and keep their shapes through lysis/fixation procedures. We describe how to place live cells in a fibrin clot and how subsequently to perfuse them.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Forer A,Pickett-Heaps Jdoi
10.1016/j.cellbi.2005.04.010keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
721-30issue
9eissn
1065-6995issn
1095-8355pii
S1065-6995(05)00094-6journal_volume
29pub_type
杂志文章abstract::Inflammatory cytokines exert different effects on hematopoietic stem cells (HSCs), lead to the development of various cell lineages in bone marrow (BM) and are thus a differentiation axis for HSCs. The content used in this article has been obtained by searching PubMed database and Google Scholar search engine of Engli...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.11545
更新日期:2021-01-02 00:00:00
abstract::Almost universally, the effective stimulus for mammalian 9+0 primary cilia has been taken to be bending. In this article I point out that in several physiological contexts there is great advantage in detecting pressure differences across the cell wall, i.e. axially directed forces pushing fluid to and fro through the ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1016/j.cellbi.2008.03.001
更新日期:2008-04-01 00:00:00
abstract::The mitogenic effect of TGF-alpha, acidic-FGF, basic-FGF and lithium on normal human breast epithelial cells was studied in a collagen gel culture system using a serum-free 1:1 mixture of Ham's F12 and DME medium containing insulin, cholera toxin and bovine serum albumin. TGF-alpha elicited a strong mitogenic response...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1077
更新日期:1993-04-01 00:00:00
abstract::We have monitored the effects of KLKL(5)KLK (KLK), a derivative of a natural cationic antimicrobial peptide (CAP) on isolated membrane vesicles, and investigated the partition of the peptide within these structures. KLK readily interacted with fluorescent dyes entrapped in the vesicles without apparent pore formation....
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.08.024
更新日期:2008-11-01 00:00:00
abstract::The dynamic state of post-translational acetylation of eukaryotic histones is maintained by histone acetyltransferases (HATs) and histone deacetylases (HDACs). HATs and HDACs have been shown to be components of various regulatory protein complexes in the cell. Their enzymatic activities, intracellular localization and...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(02)90933-9
更新日期:2002-01-01 00:00:00
abstract::MicroRNAs (miRNAs) play an increasingly important role in cancer growth by coordinately suppressing genes that control cell migration, proliferation, and invasion. The above results can be achieved through the regulation of gene expression by miRNAs by suppressing translation or the direct sequence-specific degradatio...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10754
更新日期:2017-12-01 00:00:00
abstract::The rates of intracellular protein degradation, of identically labelled populations of proteins, were compared in hepatocytes cultured at 37 degrees (on an adsorbed collagen layer) and in cells preserved on gelatin gels at 10 degrees C. The half-lives of the long-lived proteins were 35.4+/-8.6 h (N=4) and 692.9+/-216....
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0333
更新日期:1999-01-01 00:00:00
abstract::CLIC5 (chloride intracellular channel 5) is a CLIC (chloride intracellular channel) with various functions. Its high expression in skeletal muscle and association with actin-based cytoskeleton suggests that it may play an important role in muscle tissue. This study was conducted to examine whether CLIC5 regulates the ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090334
更新日期:2010-03-08 00:00:00
abstract::Vascular endothelial growth factor (VEGF) plays a key role in formation of pleural effusions and in tumorigenesis and progression of malignant mesothelioma. Mesothelial cells (MC) express the viral receptors Toll-like receptor 3 (TLR3), RIG-I and MDA5. Activation of these receptors by viral RNA exemplified by poly(I:C...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.10.011
更新日期:2009-02-01 00:00:00
abstract::Ca(2+)mobilization induced by ATP, isoproterenol and the Ca(2+)-ATPase inhibitor thapsigargin in the human submandibular duct cell line A253 was investigated using the Ca(2+)-sensitive fluorescent indicator fura-2. ATP and isoproterenol increased cytosolic free Ca(2+)([Ca(2+)](i)) and subsequent exposure to thapsigarg...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0503
更新日期:2000-01-01 00:00:00
abstract::The aim of this study was to examine the effects of glafenine hydrochloride (a nonsteroidal anti-inflammatory drug) on proliferation, clonogenic activity, cell-cycle, migration, and the extracellular matrix protein tenascin of human aortic smooth muscle cells (haSMCs) and human endothelial cells (ECs) in vitro.HaSMCs ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2003.09.001
更新日期:2003-01-01 00:00:00
abstract::Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC-R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC-R1/E and EBs were allowed to attach to the bottoms of normal cell-culturing plate and grow. Then, different...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10380
更新日期:2014-12-01 00:00:00
abstract::DCs (dendritic cells) are the strongest professional APCs (antigen-presenting cells) to initiate immune responses against pathogens, but they are usually incompetent in initiating efficient immune responses in the progress of solid tumours. We have shown that Notch signalling plays a pivotal role in DC-dependent anti-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100462
更新日期:2012-03-01 00:00:00
abstract::The neuropathology associated with Parkinson's disease (PD) is thought to involve excessive production of free radicals, dopamine autoxidation, defects in glutathione peroxidase expression, attenuated levels of reduced glutathione, altered calcium homeostasis, excitotoxicity and genetic defects in mitochondrial comple...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.03.005
更新日期:2004-01-01 00:00:00
abstract::Heparin-binding epidermal growth factor (HB-EGF) is a member of highly conserved superfamily of proteins that has potential mitogenic activity and stimulates differentiation and migration of various cell types. Since HB-EGF has three intra-molecular disulfide bonds, a high expression pattern of active HB-EGF in an E. ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10454
更新日期:2015-07-01 00:00:00
abstract::CO25 cells, a mouse myoblast line, contain multiple centrioles and primary cilia. A most unusual feature has been the finding of large numbers of separate structures in single cells-up to a maximum of nine centrioles, six primary cilia, and 12 of both organelles together. Aberrant multipolar spindles were occasionally...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0103
更新日期:1996-12-01 00:00:00
abstract::Calcium loss and muscle atrophy are two of the main metabolic changes experienced by astronauts and crew members during exposure to microgravity in space. Calcium and cytoskeletal events were investigated within sea urchin embryos which were cultured in space under both microgravity and 1 g conditions. Embryos were fi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0371
更新日期:1999-01-01 00:00:00
abstract::The purpose of the study was to examine mechanisms controlling cell cycle progression/arrest and differentiation of mouse C2C12 myoblasts exposed to long-chain saturated fatty acid salt, palmitate. Treatment of proliferating myoblasts with palmitate (0.1 mmol/l) markedly decreased myoblast number. Cyclin A and cyclin ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10477
更新日期:2015-09-01 00:00:00
abstract::We studied the genotoxic potential of a carcinogen in the human hepatoma cell line, HepG2, and in primary rat hepatocyte culture. HepG2 is a well differentiated human hepatoblastoma cell line with biotransforming capacity. Rat hepatocytes were obtained by the standard two-step in situ perfusion technique. Following be...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1083
更新日期:1993-04-01 00:00:00
abstract::The efficacies of chemotherapeutic agents are often limited by side effects and acquired drug resistance. We have investigated whether the differential expression pattern of 14-3-3σ affects cisplatin response in non-small cell lung cancer cell lines. Two pairs of parental/cisplatin resistant cell lines (A549/CRA549 an...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10006
更新日期:2013-01-01 00:00:00
abstract::FAM107B expression was decreased in stomach cancer and many other kinds of cancer. The forced expression of FAM107B in HeLa cells diminished proliferation in response to growth factors, suggesting that FAM107B might play important roles in many types of cancers. But the mechanisms underlying the decreased expression o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10816
更新日期:2017-10-01 00:00:00
abstract::The mdx mouse has been used as an animal model for human Duchenne muscular dystrophy (DMD). Unlike DMD, skeletal muscles of mdx mice undergo successful regeneration and do not show extensive fibrosis and functional impairment. Growth factors have been proposed to be involved in muscle growth and regeneration. We compa...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1068
更新日期:1994-04-01 00:00:00
abstract::Inostamycin, which was recently isolated from Streptomyces sp. MH816-AF15 as an inhibitor of cytidine 5'-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG): inositol transferase, caused a G1-phase accumulation in the cell cycle of small cell lung carcinomas. To investigate whether the cytostatic effect of inostamycin is rest...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0706
更新日期:2001-01-01 00:00:00
abstract::An acetylcholine (ACh) agonist, carbachol (Cch), causes hepatocytes to proliferate in the presence of hepatic nonparenchymal cells (HNPCs). To identify the HNPCs and ACh receptor subtypes involved in carbachol-induced hepatocyte proliferation (CIHP), we examined two types of vascular cells as candidates for HNPCs medi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.01.018
更新日期:2009-04-01 00:00:00
abstract::The interaction of NO and O2- free radicals generated from PMA (phorbol myristate acetate)-stimulated PMN (polymorphonuclear leukocytes) was studied by a nitroxide spin trap, DMPO (5,5-dimethyl-1-pyrroline-1-oxide). It was found that addition of L-arginine to the system would significantly decrease the trapped O2- by ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0039
更新日期:1996-05-01 00:00:00
abstract::Wnt signalling regulates cell proliferation and cell fate determination during embryogenesis. However, little is known about the developmental role of one Wnt family member, Wnt-3, during avian development. To investigate the possible functions of Wnt-3, its expression pattern was determined using whole-mount in situ ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090133
更新日期:2010-07-01 00:00:00
abstract::Osteosarcoma (OS) is the most common type of primary bone tumor that exhibits invasive growth and long-distance organ metastasis. Thus, investigating the specifically targeted therapeutic agents against metastatic osteosarcoma depends on understanding the molecular mechanisms. The long noncoding RNAs (lncRNA) XIST (X-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11405
更新日期:2020-10-01 00:00:00
abstract::The synthesis of proteins from salt-tolerant Brassica oleracea L. var. botrytis L. subvar. cauliflora (Gars.) DC. (cauliflower) cell cultures is modified in relation to controls in several features. There are nine newly induced polypeptides in tolerant cultures (absent in control conditions). Some of them are only pre...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1146
更新日期:1993-09-01 00:00:00
abstract::Long noncoding RNAs (lncRNAs) play important roles in diverse cellular processes and carcinogenesis. Homeobox A cluster antisense RNA 2 (HOXA-AS2) is a 1,048-basepairs lncRNA located between human HOXA3 and HOXA4 genes, whose overactivation was previously found to promote the proliferation and invasion of solid tumors...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11370
更新日期:2020-08-01 00:00:00
abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.01.011
更新日期:2008-01-25 00:00:00