Fibrin clots keep non-adhering living cells in place on glass for perfusion or fixation.

Abstract:

:We describe a method to hold living cells in place that ordinarily do not adhere to glass coverslips. The method, developed for insect spermatocytes but with application to other cell types, consists of embedding cells in a fibrin clot that forms after the enzyme thrombin cleaves the blood protein fibrinogen. The method permits continuous observation of living cells as they are treated with and recover from drug or other treatments: when held in the clot the living cells remain in place and keep their shapes when perfused with drugs that ordinarily cause drastic shape changes, and they remain in place and keep their shapes through lysis/fixation procedures. We describe how to place live cells in a fibrin clot and how subsequently to perfuse them.

journal_name

Cell Biol Int

authors

Forer A,Pickett-Heaps J

doi

10.1016/j.cellbi.2005.04.010

keywords:

subject

Has Abstract

pub_date

2005-09-01 00:00:00

pages

721-30

issue

9

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(05)00094-6

journal_volume

29

pub_type

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