Abstract：
:The aim of this study was to examine the effects of glafenine hydrochloride (a nonsteroidal anti-inflammatory drug) on proliferation, clonogenic activity, cell-cycle, migration, and the extracellular matrix protein tenascin of human aortic smooth muscle cells (haSMCs) and human endothelial cells (ECs) in vitro.HaSMCs and ECs were seeded in tissue culture flasks. The cells were treated for 4 days with glafenine hydrochloride (10 microM, 50 microM, 100 microM). Half of the treated groups were incubated again with glafenine hydrochloride, the other half received medium free of glafenine hydrochloride every 4 days until day 20. The growth kinetics and clonogenic activity were assessed. Cell cycle distribution was investigated by FACS, migratory ability was evaluated, and effects on extracellular matrix synthesis were assessed by immunofluorescence. Glafenine hydrochloride inhibited the proliferation and clonogenic activity of haSMCs and ECs in a dose-dependent manner. A block in the G2/M phase and a reduction in the G1 phase occurred. The migratory ability of haSMCs was impaired in a dose-dependent manner and the extracellular matrix protein tenascin was reduced. As glafenine hydrochloride has the ability to fully inhibit proliferation and to partially inhibit migration in haSMCs, it could be an interesting substance for further research in the field of restenosis therapy.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Schöber W,Tran QB,Muringaseril M,Wiskirchen J,Kehlbach R,Rodegerdts E,Wiesinger B,Claussen CD,Duda SHdoi
10.1016/j.cellbi.2003.09.001keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
987-96issue
12eissn
1065-6995issn
1095-8355pii
S1065699503002166journal_volume
27pub_type
杂志文章abstract：:Photodynamic therapy (PDT) for cancer is a therapeutic modality in the treatment of tumors in which visible light is used to activate a photosensitizer. Cell membranes have been identified as an important intracellular target for singlet oxygen produced during the photochemical pathway. This study analyzed the cytotox...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2008.04.005
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2000.0583
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journal_title：Cell biology international
pub_type： 杂志文章
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journal_title：Cell biology international
pub_type： 杂志文章
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1999.0423
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1042/CBI20100624
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2002.0887
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2004.12.006
更新日期：2005-03-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11015
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journal_title：Cell biology international
pub_type： 杂志文章
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journal_title：Cell biology international
pub_type： 杂志文章
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更新日期：2007-11-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.03.010
更新日期：2007-09-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.03.006
更新日期：2007-09-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2006.11.026
更新日期：2007-05-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.01.008
更新日期：2007-07-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2005.10.011
更新日期：2005-12-01 00:00:00
abstract：:We have previously reported that the T cell line Jurkat registers the exposure of a sinusoidal extremely low frequency magnetic field at the level of the plasma membrane, resulting in activation of the tyrosine kinase p56(lck), increase in inositol-3-phosphate levels and increase in intracellular calcium concentration...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.2001.0837
更新日期：2002-01-01 00:00:00
abstract：:The exon 16-encoded juxtamembrane (JM) domain of human insulin receptor (hIR) harbors the NPEY motif which couples the insulin-activated hIR kinase to downstream signal transduction molecules. We sought to determine if signal transduction requires the entire exon 16-encoded 22-amino acid JM domain. Transfected CHO cel...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.01.033
更新日期：2007-08-01 00:00:00
abstract：:Peritoneal dialysis (PD) is often used to treat patients with end stage renal disease, and its long-term complications include excessive inflammation and oxidative stress. Allograft inflammatory factor 1 (AIF-1), as a cytoplasmic protein, is originally identified from infiltrating macrophages, and it was associated wi...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.11115
更新日期：2019-05-01 00:00:00
abstract：:Most hepatomas have a defect in prothrombin carboxylation, and can secrete under-carboxylated prothrombin or des-gamma-carboxy-prothrombin (DCP), the function of which is unknown. We considered that the prothrombin-DCP axis might also be involved in growth control. Hepatocytes and hepatoma cells were treated with prot...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.03.001
更新日期：2007-09-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10116
更新日期：2013-09-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章,评审
doi：10.1002/cbin.10764
更新日期：2017-06-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
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更新日期：2009-09-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1002/cbin.10678
更新日期：2016-12-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2007.01.009
更新日期：2007-08-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1016/j.cellbi.2005.03.008
更新日期：2005-07-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1042/CBI20100919
更新日期：2012-08-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1998.0231
更新日期：1998-01-01 00:00:00
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journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1995.1061
更新日期：1995-03-01 00:00:00
abstract：:The mdx mouse has been used as an animal model for human Duchenne muscular dystrophy (DMD). Unlike DMD, skeletal muscles of mdx mice undergo successful regeneration and do not show extensive fibrosis and functional impairment. Growth factors have been proposed to be involved in muscle growth and regeneration. We compa...
journal_title：Cell biology international
pub_type： 杂志文章
doi：10.1006/cbir.1994.1068
更新日期：1994-04-01 00:00:00
