Na+/H+ exchanger 1 inhibition contributes to K562 leukaemic cell differentiation.

Abstract:

:The effect of hypoxia on the differentiation of chronic myeloid leukaemic K562 cells were studied, as was the role of the NHE1 (Na+/H+ exchanger 1). Hypoxia induced differentiation of K562 cells as seen by modifications in their morphological features, up-regulation of C/EBPα (CCAAT/enhancer-binding protein α), and marked IL-8 (interleukin-8) release. Inhibition of NHE1 under hypoxia additionally enhanced the level of C/EBPα and further promoted leukaemic cells differentiation. Pharmacological inhibition of p38 MAPK (mitogen-activated protein kinase) also significantly suppressed C/EBPα expression under hypoxia conditions after NHE1 inhibition. These results indicate the enhancement of hypoxia-induced K562 differentiation by NHE1 inhibition, which may be due to up-regulation of C/EBPα via p38 MAPK signalling pathway, which suggests a possible therapeutic target of NHE1 under hypoxia microenvironment in the treatment of leukaemic diseases.

journal_name

Cell Biol Int

authors

Jin W,Li Q,Wang J,Chang G,Lin Y,Li H,Wang L,Gao W,Pang T

doi

10.1042/CBI20100919

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

739-45

issue

8

eissn

1065-6995

issn

1095-8355

pii

CBI20100919

journal_volume

36

pub_type

杂志文章
  • MicroRNA-140-5p inhibits the tumorigenesis of oral squamous cell carcinoma by targeting p21-activated kinase 4.

    abstract::Oral squamous cell carcinoma (OSCC) is a serious global health problem. Recently, accumulating microRNA (miRNA) has emerged as crucial players in the development and progression of carcinomas including OSCC. Our study aimed to further investigate the roles of miR-140-5p in OSCC tumorigenesis and related molecular basi...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11213

    authors: Peng M,Pang C

    更新日期:2019-08-08 00:00:00

  • Effect of estrogen on chick primordial follicle development and activation.

    abstract::Steroid hormones and their receptors play pivotal roles in avian reproduction and development. 17β-estradiol (E2 ) is an essential mediator in ovarian development. In this study, Day 3 (D3) chicks were injected with E2 (0.025, 0.25 or 2.5 mg/kg body weight) for 4 days to assess the effects of estrogen on avian follicl...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10766

    authors: Zhao D,Lv C,Liu G,Mi Y,Zhang C

    更新日期:2017-06-01 00:00:00

  • K562 cells display different vulnerability to H₂O₂ induced oxidative stress in differing cell cycle phases.

    abstract::Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temp...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10360

    authors: Akcakaya H,Dal F,Tok S,Cinar SA,Nurten R

    更新日期:2015-02-01 00:00:00

  • Aphidicolin inhibits DNA replication in the micronucleus and blocks cytokinesis in Paramecium caudatum.

    abstract::Aphidicolin, a selective inhibitor of DNA polymerase, totally blocks DNA replication in the micronucleus but not in the macronucleus of Paramecium caudatum. The ciliates no longer divide and after 4 days the DNA content of the macronucleus has increased by 64%. Concomitantly the cell volume has increased by 53%. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0482

    authors: Sabaneyeva E,Tao W,Verbelen JP

    更新日期:1999-01-01 00:00:00

  • Effects of coumarin and 7OH-coumarin on bcl-2 and Bax expression in two human lung cancer cell lines in vitro.

    abstract::Coumarin and its derivative 7-hydroxycoumarin (7-OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl-2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu-1. The cells were cultured in vitro...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2005.04.003

    authors: Elinos-Báez CM,León F,Santos E

    更新日期:2005-08-01 00:00:00

  • Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells.

    abstract::Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1994.1029

    authors: Kaeffer B,Uriel IG,Bottreau E

    更新日期:1994-11-01 00:00:00

  • Cytoskeleton during aerenchyma formation in plants.

    abstract::Aerenchyma is a plant tissue characterized by prominent intercellular spaces facilitating gas diffusion between roots and the aerial environment. The classical formation of intercellular spaces is thought to be the result of schizogeny and lysogeny during development of wetland species and in some dry-land species in ...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10814

    authors: Kordyum EL,Shevchenko GV,Brykov VO

    更新日期:2019-09-01 00:00:00

  • Expression and immunohistochemical localization of galectin-3 in various mouse tissues.

    abstract::The expression and immunohistochemical localization of galectin-3, a beta-galactoside-binding protein, was studied in several mouse tissues. Galectin-3 expression was low in the cerebrum, heart, and pancreas, and moderate in the liver, ileum, kidney, and adrenal gland. High expression of galectin-3 was found in the lu...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.11.036

    authors: Kim H,Lee J,Hyun JW,Park JW,Joo HG,Shin T

    更新日期:2007-07-01 00:00:00

  • The EGFP/hERG fusion protein alter the electrophysiological properties of hERG channels in HEK293 cells.

    abstract::EGFP (enhanced green fluorescent protein) tagged to either the N (amino)-terminus [EGFP/hERG (human ether-a-go-go-related gene)] or C (carboxyl)-terminus (hERG/EGFP) of hERG channel is used to study mutant channel protein trafficking for several years. However, it has been reported that the process can alter hERG chan...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100022

    authors: Huang N,Lian JF,Huo JH,Liu LY,Ni L,Yang X,Zhou JQ,Li ZF,Song TS,Huang C

    更新日期:2011-03-01 00:00:00

  • Taxol induced Bcl-2 protein phosphorylation in human hepatocellular carcinoma QGY-7703 cell line.

    abstract::Bcl-2 family proteins play a critical role in the regulation of apoptosis. Treatment of a human hepatocellular carcinoma cell line, QGY-7703, with Taxol induced apoptosis and Bcl-2 protein phosphorylation. Microscopic observation indicated that apoptotic bodies (0-15%) of Taxol-treated QGY cells appeared after 12 h of...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0619

    authors: Cheng SC,Luo D,Xie Y

    更新日期:2001-01-01 00:00:00

  • Mechanism of low-temperature-induced pollen failure in rice.

    abstract::Low-temperature stress during microspore development alters cellular organization in rice anthers. The major cellular damage includes unusual starch accumulation in the plastids of the endothecium in postmeiotic anthers, abnormal vacuolation and hypertrophy of the tapetum, premature callose (1,3-beta-glucan) breakdown...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090417

    authors: Al Mamun E,Cantrill LC,Overall RL,Sutton BG

    更新日期:2010-03-29 00:00:00

  • Exogenous hydrogen sulfide protects fatty liver against ischemia-reperfusion injury by regulating endoplasmic reticulum stress-induced autophagy in macrophage through mediating the class A scavenger receptor pathway in rats.

    abstract::Fatty liver disease is a disease manifested with excessive alcohol intake and obese. Importantly, hydrogen sulfide (H2 S) has been revealed to participate in the progression of fatty liver; however, the underlying mechanism has not been clearly elucidated yet. In this study, we aimed to investigate the effects of exog...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11234

    authors: Ruan Z,Liang M,Deng X,Lai M,Shang L,Su X

    更新日期:2019-09-04 00:00:00

  • Localization of pan-cadherin immunoreactivity in adult rat tissues.

    abstract::Cadherins, being responsible for selective cell recognition and normal tissue integrity in adults, regulate morphogenesis in a variety of organs during development. In this study, anti-rat pan-cadherin antibody, specific to all subgroups of the cadherin family, was used to map the distribution of the pan-cadherin immu...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2002.0956

    authors: Filiz S,Dalcik H,Yardimoglu M,Gonca S,Ceylan S

    更新日期:2002-01-01 00:00:00

  • Uptake of cadmium ions in white-rot fungus Trametes versicolor: effect of cd (II) ions on the activity of laccase.

    abstract::The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2002.0887

    authors: Jarosz-Wilkolazka A,Malarczyk E,Pirszel J,Skowroński T,Leonowicz A

    更新日期:2002-01-01 00:00:00

  • Keratins and skin disorders.

    abstract::The epidermal keratinocytes express two major pairs of keratin polypeptides. One pair (K5/K14) expressed specifically in basal generative compartment and the other (K1/K10) expressed specifically in the differentiating suprabasal compartment. The switch in the expression of the keratins from proliferating to different...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:

    authors: Rao KS,Babu KK,Gupta PD

    更新日期:1996-04-01 00:00:00

  • Ro52/SSA sensitizes cells to death receptor-induced apoptosis by down-regulating c-FLIP(L).

    abstract::Ro52/SSA is an autoantigen that presents in patients with SS (Sjögren's syndrome) and SLE (systemic lupus erythematosus). It increases cell death and redistributes itself to apoptotic blebs, but its pro-apoptotic function has not been completely identified. Overexpression of Ro52/SSA promoted cell apoptosis induced by...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110322

    authors: Zhang J,Fang L,Zhu X,Qiao Y,Yu M,Wang L,Chen Y,Yin W,Hua ZC

    更新日期:2012-05-01 00:00:00

  • Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.

    abstract::Embryonic development depends on the normal invasion of trophoblast cells. Forkhead box C2 (FOXC2) is a member of Forkhead box family, which is involved in the tumor cells invasion. The aim of this study was to explore the roles of FOXC2 on the invasion of human trophoblast cells, and further study its molecular mecha...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10953

    authors: Zhang Y,Zhang Y

    更新日期:2018-07-01 00:00:00

  • Effect of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: immunocytochemistry MIB1 score and DNA ploidy and apoptosis evaluated by flow cytometry.

    abstract::Mitomycin C (MMC) treatment has been used to arrest cell proliferation but not much is known about the effect of MMC on human foreskin fibroblasts (HFF) used as feeders for human embryonic stem cells (hESC). We tested the ability of MMC to stop the proliferation of HFF and to induce apoptosis. MMC inhibited the prolif...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.11.006

    authors: Nieto A,Cabrera CM,Catalina P,Cobo F,Barnie A,Cortés JL,Barroso del Jesus A,Montes R,Concha A

    更新日期:2007-03-01 00:00:00

  • Stimulation of the side population fraction of ATDC5 chondroprogenitors by hypoxia.

    abstract::The influence of oxygen tension on the side population (SP) fraction sorted from ATDC5 chondroprogenitor cells was investigated. ATDC5 cells cultured in normoxia (20%) or hypoxia (1% oxygen) were compared. The SP fraction was significantly higher in the cells cultured in hypoxia. The gene expression of 3 ABC transport...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2009.06.009

    authors: Kishimoto KN,Oxford CL,Reddi AH

    更新日期:2009-12-01 00:00:00

  • Enhancement of fertilization by piperine in hamsters.

    abstract::The effect of piperine on the fertilization of eggs with sperm was investigated in female hamsters. They were intragastrically treated with piperine at doses of 50 and 100 mg/kg BW from day 1 through day 4 of the oestrous cycle. During piperine treatment, these females were superovulated and artificially inseminated (...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1997.0167

    authors: Piyachaturawat P,Pholpramool C

    更新日期:1997-07-01 00:00:00

  • Examination of actin polymerization and viscosity induced by cations and ionic strength when cross-linked by alpha-actinin.

    abstract::Increasing potassium chloride concentration from 0 to 100 mM and magnesium chloride from 0 to 2 mM show a parallel rate increase in polymerizing actin, whereas increasing calcium chloride concentration from 0 to 0.2 mM decreases the rate of polymerizing actin. The presence of alpha-actinin has little influence on the ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2002.0898

    authors: Goldmann WH

    更新日期:2002-01-01 00:00:00

  • MiR-139-5p Upregulation Alleviated Spontaneous Recurrent Epileptiform Discharge-induced Oxidative Stress and Apoptosis in Rat Hippocampal Neurons via Regulating the Notch Pathway.

    abstract::Epilepsy was characterized by the occurrence of spontaneous recurrent epileptiform discharges (SREDs) in neurons. Previous studies suggested that microRNA (miR)-139-5p and the Notch pathway were implicated in Epilepsy; however, their interaction remained vague. Rat primary hippocampal neurons were isolated and identif...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11509

    authors: Zhao C,Yang F,Wei X,Zhang J

    更新日期:2020-11-28 00:00:00

  • Studies on the permeability of the blood-testis barrier in stainless steel-administered mice.

    abstract::Previous trials performed in our laboratory have demonstrated that stainless steel corrosion products induced morphological changes in mice seminiferous cells. In this study the functional nature of the blood-testis barrier was investigated, in view of the extensive use of this metallic alloy in orthopaedic surgery. T...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1995.1109

    authors: Pereira ML

    更新日期:1995-07-01 00:00:00

  • Effects of adenosine and epidermal growth factor on the growth of mouse mammary epithelial cells.

    abstract::The objective of this study was to determine if adenosine alters growth of mammary epithelium. Mouse mammary epithelial cells (NMuMG) were cultured in DMEM supplemented with 10% fetal calf serum. After serum starvation for 24 h, EGF (0-100 ng/ml) and/or adenosine (0-100 microM) was added. Adenosine at concentrations o...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1998.0231

    authors: Yuh IS,Jang BB,Hong BJ

    更新日期:1998-01-01 00:00:00

  • CCN5 inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma cells.

    abstract::Oral squamous cell carcinoma (OSCC) is a common cancer with poor prognosis and high mortality. The role of CCN5 has attracted a great focus on the regulation of cancer progression. However, the biological function and mechanism of CCN5 in OSCC are still not well elucidated. The current study was designed to determine ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11296

    authors: Sun S,Cui Z,Yan T,Wu J,Liu Z

    更新日期:2020-04-01 00:00:00

  • Effects of overexpression of Sim2 on spatial memory and expression of synapsin I in rat hippocampus.

    abstract::The single-minded 2 gene (Sim2) plays a crucial role in the mental retardation of Down Syndrome (DS). To explore how Sim2 influences spatial memory, a DNA plasmid-encoding mouse Sim2 (mSim2) wrapped with liposome was bilaterally injected into the hippocampus of rats. The effect of overexpressing mSim2 on spatial learn...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.07.003

    authors: Meng X,Peng B,Shi J,Zheng Y,Chen H,Zhang J,Li L,Zhang C

    更新日期:2006-10-01 00:00:00

  • Apelin: an endogenous peptide essential for cardiomyogenic differentiation of mesenchymal stem cells via activating extracellular signal-regulated kinase 1/2 and 5.

    abstract::Growing evidence has shown that apelin/APJ system functions as a critical mediator of cardiac development as well as cardiovascular function. Here, we investigated the role of apelin in the cardiomyogenic differentiation of mesenchymal stem cells derived from Wharton's jelly of human umbilical cord in vitro. In this r...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10581

    authors: Wang L,Zhu ZM,Zhang NK,Fang ZR,Xu XH,Zheng N,Gao LR

    更新日期:2016-05-01 00:00:00

  • Phosphoserine promotes osteogenic differentiation of human adipose stromal cells through bone morphogenetic protein signalling.

    abstract::Phosphoserine has potential effectiveness as a simple substrate in preparing bone replacement materials, which could enhance bone forming ability. However, there is a need to investigate the independent effect of phosphoserine on osteogenic differentiation of human adipose stem cells (hADSCs). hADSCs were cultured in ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10203

    authors: Ying X,Chen X,Cheng S,Guo X,Chen H,Xu HZ

    更新日期:2014-03-01 00:00:00

  • Influence of membrane physical state on lysosomal potassium ion permeability.

    abstract::Lysosomal permeability to potassium ions is an important property of the organelle. Influence of the membrane physical state on the potassium ion permeability of isolated lysosomes was assessed by measuring the membrane potential with bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol and monitoring the lysosomal pro...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.12.002

    authors: Wang J,Zhang GJ

    更新日期:2005-06-01 00:00:00

  • Inflammation: a novel mechanism for the transport of extracellular nucleotide-induced arachidonic acid by S100A8/A9 for transcellular metabolism.

    abstract::Extracellular nucleotides cause neutrophil degranulation by activating the purinergic receptor subtype P2Y. However, the molecular mechanism involved in the signal pathway remains unknown. A hypothetical scheme suggesting that leukotriene(s) and leukotriene receptor(s) activation is required for extracellular nucleoti...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/s1065-6995(03)00082-9

    authors: Kannan S

    更新日期:2003-01-01 00:00:00