Nuclear deformation characterizes Werner syndrome cells.


:Mutations in the lamin A gene have been shown, among other defects, to give rise to Hutchinson-Gilford progeria syndrome (HGPS) and to atypical Werner syndrome (WS), both of which are progeroid disorders. Here, we have investigated well-characterized WS patient cell strains that are compound heterozygous for mutations in the WRN gene. As in HGPS and in atypical WS, we found nuclear deformations to be characteristic of all cell strains studied. In WS cells centrosome number, assembly of the nuclear lamina and nuclear pore distribution occurred normally. Furthermore, nuclear deformations were not associated with a defect in lamin A expression. We propose that nuclear deformation is a universal characteristic of progeroid cells and may result from slow cell cycle progression.


Cell Biol Int


Adelfalk C,Scherthan H,Hirsch-Kauffmann M,Schweiger M





Has Abstract


2005-12-01 00:00:00














  • Effect of downregulation of ZEB1 on vimentin expression, tumour migration and tumourigenicity of melanoma B16F10 cells and CSCs.

    abstract::Zinc-finger E-box binding homeobox 1 (ZEB1) is a master regulator of epithelial-mesenchymal transition (EMT) and has been implicated in primary epithelial cancer biological processes, such as invasion and metastasis. However, the role of ZEB1 in progression of melanoma and cancer stem cells (CSCs) remains obscure. In ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Dou J,He X,Liu Y,Wang Y,Zhao F,Wang X,Chen D,Shi F,Wang J

    更新日期:2014-04-01 00:00:00

  • Expression patterns of transcription factor PPARγ and C/EBP family members during in vitro adipogenesis of human bone marrow mesenchymal stem cells.

    abstract::In the past decades increasing lines of evidence have demonstrated that adipose tissue, as an endocrine organ plays a central role in metabolic homeostasis and its related maladies. CCAAT/enhancer-binding protein (C/EBP) family members and the nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ) w...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhao XY,Chen XY,Zhang ZJ,Kang Y,Liao WM,Yu WH,Xiang AP

    更新日期:2015-04-01 00:00:00

  • Development of transformation vectors based upon a modified plant alpha-tubulin gene as the selectable marker.

    abstract::A plant transformation and selection system has been developed utilizing a modified tubulin gene as a selectable marker. The vector constructs carrying a mutant alpha-tubulin gene from goosegrass conferring resistance to dinitroaniline herbicides were created for transformation of monocotyledonous and dicotyledonous p...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Yemets A,Radchuk V,Bayer O,Bayer G,Pakhomov A,Vance Baird W,Blume YB

    更新日期:2008-05-01 00:00:00

  • A fine structural study of the hippocampus and dorsal root ganglion in mouse trisomy 16, a model of Down's syndrome.

    abstract::Mouse trisomy 16 (Ts16) appears to provide an animal model of Down's syndrome in that a portion of mouse chromosome 16 is syntenic with part of human chromosome 21. Trisomy 21 in human beings leads to the mental retardation of Down's syndrome and in middle age, to some presenile anatomic and clinical features of Alzhe...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Lane NJ,Balbo A,Rapoport SI

    更新日期:1996-10-01 00:00:00

  • Bradykinin receptor-1 activation induces inflammation and increases the permeability of human brain microvascular endothelial cells.

    abstract::Neuroinflammatory disorders such as Alzheimer's and Parkinson's diseases are characterised by chronic inflammation and loss of vascular integrity. Bradykinin 1 receptor (B1R) activation has been implicated in many neuroinflammatory diseases, but the contribution of B1R to inflammation and vascular breakdown is yet to ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Mugisho OO,Robilliard LD,Nicholson LFB,Graham ES,O'Carroll SJ

    更新日期:2019-09-09 00:00:00

  • Inhibition of acid-sensing ion channels by amiloride protects rat articular chondrocytes from acid-induced apoptosis via a mitochondrial-mediated pathway.

    abstract::A significant decrease in tissue pH or acidosis is a common feature of numerous diseases, including RA (rheumatoid arthritis). Cartilage homoeostasis is profoundly affected by local acidosis in the joints. The diuretic, amiloride, is neuroprotective in models of cerebral ischaemia, a property attributable to the inhib...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Rong C,Chen FH,Jiang S,Hu W,Wu FR,Chen TY,Yuan FL

    更新日期:2012-07-01 00:00:00

  • Effect of silicon dioxide on expression of poly (ADP-ribose) polymerase mRNA and protein.

    abstract::Silicon dioxide induces acute injury and chronic pulmonary fibrosis. International Agency for Research on Cancer (IARC) listed it as a human carcinogen in 1996. However, the molecular mechanisms to induce cancer are not understood yet. The content of poly (ADP-ribose) polymerases (PARP) mRNA and protein in Hela cells ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Gao A,Song S,Wang D,Peng W,Tian L

    更新日期:2009-07-01 00:00:00

  • miR-483-3p plays an oncogenic role in esophageal squamous cell carcinoma by targeting tumor suppressor EI24.

    abstract::microRNAs (miRNAs), through negatively regulating their target genes, influence the development and progression of many cancers. Previously, we found miR-483 was overexpressed in esophageal squamous cell carcinoma (ESCC) tissues, and its overexpression was negatively correlated with the prognosis and positively correl...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Ma J,Hong L,Xu G,Hao J,Wang R,Guo H,Liu J,Zhang Y,Nie Y,Fan D

    更新日期:2016-04-01 00:00:00

  • An appreciation of the prescience of Don Gilbert (1930-2011): master of the theory and experimental unravelling of biochemical and cellular oscillatory dynamics.

    abstract::We review Don Gilbert's pioneering seminal contributions that both detailed the mathematical principles and the experimental demonstration of several of the key dynamic characteristics of life. Long before it became evident to the wider biochemical community, Gilbert proposed that cellular growth and replication neces...

    journal_title:Cell biology international

    pub_type: 传,历史文章,杂志文章


    authors: Gilbert K,Hammond KD,Brodsky VY,Lloyd D

    更新日期:2020-06-01 00:00:00

  • Mesenchymal stem cell based therapy for osteo-diseases.

    abstract::Stem cell therapy in recent years has gained much attention as the modern therapeutic approach to treat diseases. Mesenchymal stem cells (MSCs) are seen as the most reliable cells applied in therapy over other stem cells because of their versatility. Bone and cartilage diseases (osteo-diseases) are the major target of...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审


    authors: Ghasroldasht MM,Irfan-Maqsood M,Matin MM,Bidkhori HR,Naderi-Meshkin H,Moradi A,Bahrami AR

    更新日期:2014-10-01 00:00:00

  • mGlu3 receptor blockade inhibits proliferation and promotes astrocytic phenotype in glioma stem cells.

    abstract::We have characterised, using both in vivo and in vitro methods, the effects of the metabotropic glutamate receptor subtype 3 (mGlu3) antagonist (LY341495) and agonist (LY379268) on the proliferation and differentiation of glioma stem cells (GSC). For in vitro studies, a CCK-8 assay was used to determine the cell proli...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhou K,Song Y,Zhou W,Zhang C,Shu H,Yang H,Wang B

    更新日期:2014-04-01 00:00:00

  • Peroxisomicine A1, a potential antineoplastic agent, causes micropexophagy in addition to macropexophagy.

    abstract::Peroxisomicine A1 (PA1) is a potential antineoplastic agent with high and selective toxicity toward peroxisomes of tumor cells. Pexophagy is a selective autophagy process that degrades damaged peroxisomes; this process has been studied mainly in methylotrophic yeasts. There are two main modes of pexophagy in yeast: ma...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Ortega-Martínez M,Gutiérrez-Dávila V,Niderhauser-García A,Salazar-Aranda R,Solís-Soto JM,Montes-de-Oca-Luna R,Jaramillo-Rangel G

    更新日期:2020-03-01 00:00:00

  • Expression analysis of Fgf8a &Fgf8b in early stage of P19 cells during neural differentiation.

    abstract::Fgf8 is a member of the fibroblast growth factor (FGF) family that plays an important role in early neural development. Cellular aggregation and retinoic acid (RA) are needed for mouse embryonic carcinoma (EC) P19 cell neural differentiation. We have examined the Fgf8 gene in P19 cells during neural differentiation an...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Alam AH,Suzuki H,Tsukahara T

    更新日期:2009-09-01 00:00:00

  • Expression of primary cilia in mammalian cells.

    abstract::Mammalian cells in vivo frequently express primary cilia. Although some fully differentiated cell types rarely, if ever, express them, most do, indicating that they are regular cell organelles. Their expression can also be explored in vitro, where conditions--physical and chemical, intrinsic and extrinsic--permit expe...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审


    authors: Wheatley DN,Wang AM,Strugnell GE

    更新日期:1996-01-01 00:00:00

  • Role of 14-3-3σ in resistance to cisplatin in non-small cell lung cancer cells.

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    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Cetintas VB,Tetik A,Cok G,Kucukaslan AS,Kosova B,Gunduz C,Veral A,Eroglu Z

    更新日期:2013-01-01 00:00:00

  • 3D arrays for high throughput assay of cell migration and electrotaxis.

    abstract::Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhao S,Gao R,Devreotes PN,Mogilner A,Zhao M

    更新日期:2013-09-01 00:00:00

  • Bcl-2 down-regulation by small interfering RNA induces Beclin1-dependent autophagy in human SGC-7901 cells.

    abstract::While Bcl-2 protein is involved in the regulation of apoptosis, recent research showed that Beclin1, described as the essential autophagy effector and haploinsufficient tumor suppressor, was originally isolated as a Bcl-2 interacting protein. Beclin1 interacts with Bcl-2 through a BH3 domain; nevertheless, the functio...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Du Y,Ji X

    更新日期:2014-10-01 00:00:00

  • Exportin 1-independent nuclear export of GAPDH.

    abstract::Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme of the glycolytic pathway. Recent studies have demonstrated an additional role in apoptosis: GAPDH is targeted to the nucleus during apoptotic signalling. This nuclear transport has also been observed in serum-depleted cells, but it is reversible in fibr...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Schmitz HD,Dutiné C,Bereiter-Hahn J

    更新日期:2003-01-01 00:00:00

  • Rosbin, a synthetic small molecule, induces A549 cells apoptosis through a ROS-mediated pathway.

    abstract::Rosbin (Thieno [2, 3-c] piperidine-3-carboxamide-2-[(3-methoxy-naphtha-lene-2-carbonyl)-amino]-6-(benzyl)-, hydrochloride), a synthetic piperidinothieno derivative compound obtained from chemical genetics screenings, significantly suppresses the viability of non-small cell lung cancer A549 cells with an IC50 of 2.05 ±...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhao T,Jin W,Pan H,Li H,Zhao Y,Feng Y

    更新日期:2017-02-01 00:00:00

  • The poly-cistronic expression of four transcriptional factors (CRX, RAX, NEURO-D, OTX2) in fibroblasts via retro- or lentivirus causes partial reprogramming into photoreceptor cells.

    abstract::The introduction of four key transcriptional factors (CRX, RAX, NEURO-D, OTX2) allows the direct differentiation of fibroblasts to retinal photoreceptor cells. This reprogramming was achieved with a combination of mono-cistronic viruses. Although the combination of mono-cistronic viruses was useful, a relatively high ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Fukuda T,Ishizawa Y,Donai K,Sugano E,Tomita H

    更新日期:2018-05-01 00:00:00

  • High-level expression and purification of soluble bioactive recombinant human heparin-binding epidermal growth factor in Escherichia coli.

    abstract::Heparin-binding epidermal growth factor (HB-EGF) is a member of highly conserved superfamily of proteins that has potential mitogenic activity and stimulates differentiation and migration of various cell types. Since HB-EGF has three intra-molecular disulfide bonds, a high expression pattern of active HB-EGF in an E. ...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Khalili M,Soleyman MR,Baazm M,Beyer C

    更新日期:2015-07-01 00:00:00

  • Long non-coding RNA MALAT1 functions as a mediator in cardioprotective effects of fentanyl in myocardial ischemia-reperfusion injury.

    abstract::Long non-coding (lncRNA) MALAT1 can be increased by hypoxia or ischemic limbs. Also, downregulation of MALAT1 contributes to reduction of cardiomyocyte apoptosis. However, the functional involvement of MALAT1 in myocardial ischemia-reperfusion (I/R) injury has not been defined. This study investigated the functional i...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhao ZH,Hao W,Meng QT,Du XB,Lei SQ,Xia ZY

    更新日期:2017-01-01 00:00:00

  • TNFR1 and TNFR2 differentially mediate TNF-α-induced inflammatory responses in rheumatoid arthritis fibroblast-like synoviocytes.

    abstract::TNF-α has long been implicated in the progression of rheumatoid arthritis (RA). However, how the receptors of TNF-α, namely TNFR1 and TNFR2, mediate TNF-α-induced inflammatory responses in fibroblast-like synoviocytes (FLS) in RA has not been elucidated. In the present study, primary FLS cells were isolated from RA pa...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Zhang H,Xiao W

    更新日期:2017-04-01 00:00:00

  • Mitotic catastrophe and endomitosis in tumour cells: an evolutionary key to a molecular solution.

    abstract::Following genotoxic insult, p53 mutated tumour cells undergo mitotic catastrophe. This is characterised by a switch from mitosis to the endocycle. The essential difference between mitosis and the endocycle is that in the latter, DNA synthesis is uncoupled from cell division, which leads to the formation of endopolyplo...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审


    authors: Erenpreisa J,Kalejs M,Cragg MS

    更新日期:2005-12-01 00:00:00

  • Nuclear antigens, as DNA, of DSB389 MAb and some other anti-desmin monoclonal antibodies.

    abstract::The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were search...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Kamei H

    更新日期:1996-11-01 00:00:00

  • Cytokeratin expression in human respiratory epithelium of nasal polyps and turbinates.

    abstract::The cytokertatins in respiratory epithelial cells (REC) of human nasal polyps and turbinates were analyzed by immunohistochemistry. Cytokeratin 19 (CK19) was present in all REC, CK5 and 14 were expressed primarily in basal cells, and CK7, 8, and 18 were found in suprabasal cells. Differences in cytoplasmic locations w...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Hicks W Jr,Ward R,Edelstein D,Hall L 3rd,Albino A,Hard R,Asch B

    更新日期:1995-04-01 00:00:00

  • Metabolic profiling of normal hepatocyte and hepatocellular carcinoma cells via 1 H nuclear magnetic resonance spectroscopy.

    abstract::Hepatocellular carcinoma (HCC) causes death mainly by disseminated metastasis progression from the organ being confined. Different metastatic stages are closely related to cellular metabolic profiles. Normal hepatocyte and HepG2 cell line from low metastatic HCC were studied by NMR-based metabolomic techniques. Multiv...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Chen Y,Chen Z,Feng JH,Chen YB,Liao NS,Su Y,Zou CY

    更新日期:2018-04-01 00:00:00

  • Comparison of the endogenous IK currents in rat hippocampal neurons and cloned Kv2.1 channels in CHO cells.

    abstract::The Kv2.1 potassium channel is a principal component of the delayed rectifier I(K) current in the pyramidal neurons of cortex and hippocampus. We used whole-cell patch-clamp recording techniques to systemically compare the electrophysiological properties between the native neuronal I(K) current of cultured rat hippoca...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Liu M,Gong B,Qi Z

    更新日期:2008-12-01 00:00:00

  • Phallacidin stains the mitotic spindle of the diatom Pinnularia spp.

    abstract::Mitotic spindles of the diatom Pinnularia viritiformis stained with the fluorescent actin-labelling reagent bodipyphallacidin revealed actin among the chromosomes and extending along the spindle to the poles. This is the first report of actin's presence within spindles of this ecologically important group of organisms...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Sampson K

    更新日期:2004-01-01 00:00:00

  • Cholesterol esterification in human monocyte-derived macrophages is inhibited by protein kinase C with dual roles for mitogen activated protein kinases.

    abstract::The possible role of protein kinase C (PKC) and mitogen activated protein (MAP) kinases in the stimulation of cholesterol esterification by acetylated low density lipoprotein (acLDL) in human monocyte-derived macrophages (HMDM) was studied. Cholesterol esterification, as assessed by the rate of incorporation of [3H]-o...

    journal_title:Cell biology international

    pub_type: 杂志文章


    authors: Napolitano M,Avella M,Goode NT,Botham KM,Bravo E

    更新日期:2004-01-01 00:00:00