Abstract:
:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme of the glycolytic pathway. Recent studies have demonstrated an additional role in apoptosis: GAPDH is targeted to the nucleus during apoptotic signalling. This nuclear transport has also been observed in serum-depleted cells, but it is reversible in fibroblasts, in contrast to apoptotic-induced transport (Eur J Cell Biol 80 (2001) 419). Here, we analyse the serum depletion-induced transport processes of GAPDH in NIH 3T3 cells. Prolonged serum depletion did not cause cell death, nuclear fragmentation (hoechst staining) or a significant increase in DNA strand-breaks (comet assay). Using cells expressing green fluorescent protein (GFP)-tagged GAPDH allowed us to monitor its intracellular localisation by confocal laser scanning microscopy (CLSM). Treatment of cells with the exportin1 inhibitor leptomycin B (LMB) did not influence cytoplasmic localisation of GFP-GAPDH, indicating that nuclear targeting of GAPDH is not constitutive and may be altered via a serum-dependent regulatory export process. Suprisingly, the export of nuclear GFP-GAPDH after re-addition of serum to starved cells was not prevented by LMB. Thus, nuclear export of GAPDH upon serum depletion is not mediated by exportin1.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Schmitz HD,Dutiné C,Bereiter-Hahn Jdoi
10.1016/s1065-6995(03)00096-9keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
511-7issue
7eissn
1065-6995issn
1095-8355pii
S1065699503000969journal_volume
27pub_type
杂志文章abstract::We analyzed the connection of changes in nucleus ploidy with changes in nucleolar apparatus of NIH 3T3 cells. The quantity of nucleoli does not depend on the quantity of nucleolar DNA, but instead depends on euploidy: the majority of euploid cells have 1-3 nucleoli. The quantity of DNA in the nucleolus is correlated w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00158-6
更新日期:2003-01-01 00:00:00
abstract::We have monitored the effects of KLKL(5)KLK (KLK), a derivative of a natural cationic antimicrobial peptide (CAP) on isolated membrane vesicles, and investigated the partition of the peptide within these structures. KLK readily interacted with fluorescent dyes entrapped in the vesicles without apparent pore formation....
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.08.024
更新日期:2008-11-01 00:00:00
abstract::The mdx mouse has been used as an animal model for human Duchenne muscular dystrophy (DMD). Unlike DMD, skeletal muscles of mdx mice undergo successful regeneration and do not show extensive fibrosis and functional impairment. Growth factors have been proposed to be involved in muscle growth and regeneration. We compa...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1068
更新日期:1994-04-01 00:00:00
abstract::Ca(2+)mobilization induced by ATP, isoproterenol and the Ca(2+)-ATPase inhibitor thapsigargin in the human submandibular duct cell line A253 was investigated using the Ca(2+)-sensitive fluorescent indicator fura-2. ATP and isoproterenol increased cytosolic free Ca(2+)([Ca(2+)](i)) and subsequent exposure to thapsigarg...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0503
更新日期:2000-01-01 00:00:00
abstract::Dexmedetomidine (Dex) protects different cell types during hypoxia or ischemia-reperfusion injury by inhibiting cell apoptosis. However, the underlying mechanism and its impact on hepatic ischemia reperfusion injury are still not known. In this study, we established a model of oxygen-glucose deprivation/reperfusion (O...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10996
更新日期:2018-09-01 00:00:00
abstract::Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which re...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0964
更新日期:2002-01-01 00:00:00
abstract::Glycolipid patterns have been studied during the first six days of Xenopus embryo development. Glycolipid contents showed a sharp increase more evident after the third day of development. Glucosylceramide and sulphatide are not only the most represented species, but also those which exhibit a statistically significant...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1061
更新日期:1995-03-01 00:00:00
abstract::DNA is constantly being damaged, either by endogenous or exogenous genotoxins. In that regard, DNA repair activities are essential for maintaining genomic stability and to life itself. Mutations in genes encoding DNA repair proteins cause severe human syndromes, but DNA repair defects have also been linked to several ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10917
更新日期:2018-06-01 00:00:00
abstract::Cytoplasmic proteins with binding capability to AU-rich instability determinant sequences (ARE) of tumour necrosis factor alpha (TNFalpha) mRNA 3' untranslated region (3'UTR) were assessed in human lymphoid cells. In vitro label transfer experiments using wild type as well as mutant sequences in which the 70 nucleotid...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0418
更新日期:1999-01-01 00:00:00
abstract::Ro52/SSA is an autoantigen that presents in patients with SS (Sjögren's syndrome) and SLE (systemic lupus erythematosus). It increases cell death and redistributes itself to apoptotic blebs, but its pro-apoptotic function has not been completely identified. Overexpression of Ro52/SSA promoted cell apoptosis induced by...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110322
更新日期:2012-05-01 00:00:00
abstract::In the present study, we investigated the effects of tetramethylpyrazine (TMP) on hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells. The apoptosis in H2O2-induced PC12 cells was accompanied by a decrease in Bcl-2/Bax protein ratio, release of cytochrome c to cytosol and the activation of caspase-3. TMP not only...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.10.001
更新日期:2007-05-01 00:00:00
abstract::This study examined whether Gd (gadolinium) could suppress prostate cancer cell migration and prostate cancer cell-induced osteoclast differentiation. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] and colony forming assay showed that GdCl3 treatment inhibited both cell viability and colony for...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100870
更新日期:2011-11-01 00:00:00
abstract::The effects of NO in its environment may vary considerably depending on various factors. This study shows oxidative mechanism of cellular membrane alterations, which is not associated with triggering of ONOOH generation but is induced by pure NO. Our investigation examined the influence of low concentration of NO (0.1...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.08.020
更新日期:2008-01-01 00:00:00
abstract::The induction and maintenance of immunologic tolerance at the feto-maternal interface is necessary for a successful pregnancy. The most accepted hypothesis for the mechanism underlying this tolerance is that pregnancy-induced foetal antigen-specific maternal T regulatory (T(reg) ) cells mediate maternal tolerance to t...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10023
更新日期:2013-02-01 00:00:00
abstract::To identify the molecular basis by which nonpermissive temperature (NPT) induces cell differentiation in Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen, we performed global scale microarray and computational gene network analyses. In TTE3 cells, inactivation of the large T-antigen by a NPT at ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.04.007
更新日期:2007-10-01 00:00:00
abstract::Mutants of the methanol-utilizing yeast Pichia pastoris and the alkane-utilizing yeast Yarrowia lipolytica defective in the orthologue of UGT51 (encoding sterol glucosyltransferase) were isolated and compared. These mutants do not contain the specific ergosterol derivate, ergosterol glucoside. We observed that the P. ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2003.08.004
更新日期:2003-01-01 00:00:00
abstract::The influence of daunorubicin (DNR) on survival of human normal (S-126) and trisomic, with respect to chromosome 21 (T-164; S-240), skin fibroblasts and some parameters related to it, such as intracellular drug accumulation, distribution and interaction with cell membrane, were studied. The in vitro growth-inhibition ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0583
更新日期:2001-01-01 00:00:00
abstract::Earlier, we have shown that spontaneously isolated non-pathogenic bacteria Serratia grimesii and Serratia proteamaculans invade eukaryotic cells, provided that they synthesize thermolysin-like metalloproteases ECP32/grimelysin or protealysin characterized by high specificity towards actin. To address the question of w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100314
更新日期:2011-02-01 00:00:00
abstract::Cell motility, cell migration and phagocytosis are distinct, though frequently sequential, processes. They are fundamental for the maintenance of homoeostasis in single cells as well as in pluricellular organisms. Like vertebrates, invertebrate immune functions are strictly dependent on cell motility, chemotaxis and p...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1042/CBI20100514
更新日期:2010-11-01 00:00:00
abstract::The aim of the present study was to investigate stress fibers and cortical actin reorganization in pubertal Sertoli cells in vitro after PMA treatment. Actin was studied by means of immunogold labeling, using the 'Progressive Lowering of Temperature' technique (PLT). Actin rearrangement was evaluated by a quantitative...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.07.008
更新日期:2005-11-01 00:00:00
abstract::Whether fructose (FRU), as the sole energy source, confers a metabolic advantage on cancer cells against noxious stimuli is unknown. The aim of this study was to evaluate the effects of low (11 mM), moderate (25 mM), and high (55 mM) FRU concentrations alone or in combination with rotenone (ROT) or doxorubicin (DOX) i...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10653
更新日期:2016-11-01 00:00:00
abstract::This report deals with observations on the directional locomotion of amoeba before and after fixation and scanning electron microscopy. The study was aimed at visualization of the stepwise events of directional movements. After the analysis of the data it is proposed that the amoeba undergoes a sequence of movement ev...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.01.009
更新日期:2007-08-01 00:00:00
abstract::Transplanted tumours could modify the intensity and temporal distribution of the cellular proliferation in normal cell populations, and partial hepatectomy alters the serum concentrations of substances involved in cellular proliferation, leading to the compensatory liver hyperplasia. The following experiments were des...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090282
更新日期:2010-02-05 00:00:00
abstract::Many disturbances in electrolyte and hormonal balance in the body induced by functional impairment of renal parenchyma may affect the activity of amine precursor uptake and decarboxylation (APUD) cells, which constitute a very important link in the regulation of homeostasis. The aim of the present study was the morpho...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.03.012
更新日期:2004-01-01 00:00:00
abstract::Pioneering single gene study documents pre-mRNA processing proteins participation in the cell-cycle regulation of multi-cellular animals (Andersen and Tapon, 2008, J Biol Chem 283: 31256-67). Whole-genome RNAi screen in Drosophila tissue-culture cell lines demonstrates that 17 genes involved in RNA-processing are requ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10019
更新日期:2013-02-01 00:00:00
abstract::The effects of melanin preparations from black yeast fungi (BYF) on the proliferation and differentiation of normal cultivated human skin keratinocytes and embryonic pulmonary fibroblasts have been investigated. Melanin preparations in the range of 5-0.1 microg/ml were optimally active, with a more pronounced effect o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(02)00297-4
更新日期:2003-01-01 00:00:00
abstract::Glutamyl aminopeptidase (GluAP, EC 3.4.11.7, ENPEP) is a 130-kDa homodimeric zinc metallopeptidase which specifically cleaves the N-terminal glutamate or aspartate residue of peptidic substrates such as cholecystokinin-8 or angiotensin (Ang) II, in vitro. We used a DNA microarray hybridization (Genechip Rat Expression...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.02.006
更新日期:2008-07-01 00:00:00
abstract::The diabetic milieu is believed to change the activity, or result in damage of podocytes-a key component of the glomerular filtration barrier and known to secrete matrix for glomerular basement membrane. This in turn contributes to diabetic nephropathy. However, how podocyte dysfunction is triggered in diabetic nephro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10363
更新日期:2015-02-01 00:00:00
abstract::Several synthetic decapeptides containing an HAV tripeptide motif were tested for their ability to modulate the enzymatic activity of rat extracellular SOD, an enzyme which also contains an HAV motif. Out of nine decapeptides that were tested, only a FGF-receptor derived peptide was active as a negative modulator of e...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1032
更新日期:1995-11-01 00:00:00
abstract::As embryonic progenitors for the gametes, PGCs (primordial germ cells) proliferate and develop under strict regulation of numerous intrinsic and external factors. As the most active natural metabolite of vitamin A, all-trans RA (retinoic acid) plays pivotal roles in regulating development of various cells. The prolife...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110542
更新日期:2012-08-01 00:00:00