Abstract:
:Aphidicolin, a selective inhibitor of DNA polymerase, totally blocks DNA replication in the micronucleus but not in the macronucleus of Paramecium caudatum. The ciliates no longer divide and after 4 days the DNA content of the macronucleus has increased by 64%. Concomitantly the cell volume has increased by 53%.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Sabaneyeva E,Tao W,Verbelen JPdoi
10.1006/cbir.1999.0482keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
859-62issue
12eissn
1065-6995issn
1095-8355pii
S1065-6995(99)90482-1journal_volume
23pub_type
杂志文章abstract::DNA is constantly being damaged, either by endogenous or exogenous genotoxins. In that regard, DNA repair activities are essential for maintaining genomic stability and to life itself. Mutations in genes encoding DNA repair proteins cause severe human syndromes, but DNA repair defects have also been linked to several ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10917
更新日期:2018-06-01 00:00:00
abstract::An acetylcholine (ACh) agonist, carbachol (Cch), causes hepatocytes to proliferate in the presence of hepatic nonparenchymal cells (HNPCs). To identify the HNPCs and ACh receptor subtypes involved in carbachol-induced hepatocyte proliferation (CIHP), we examined two types of vascular cells as candidates for HNPCs medi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.01.018
更新日期:2009-04-01 00:00:00
abstract::Klotho beta (Klb), a single-pass transmembrane protein, has been described as a co-receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this pa...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11059
更新日期:2019-02-01 00:00:00
abstract::Ras/myc-transformed serum-free mouse embryo (ras/myc SFME) cells were treated with interferon-gamma (IFN-gamma; 100 U/ml) and/or lipopolysaccharide (LPS; 0.5 microg/ml) for 24 h to simulate inflammatory and infectious conditions and investigate their effects on the expression of inducible nitric oxide synthase (iNOS) ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.008
更新日期:2008-08-01 00:00:00
abstract::Ghrelin-O-acyltransferase (GOAT) is a membrane-bound enzyme that attaches eight-carbon octanoate to a serine residue in ghrelin and thereby acylates inactive ghrelin to produce active ghrelin. In this study, we investigated the function of GOAT in the intestinal mucosal barrier. The intestinal mucosal barrier prevents...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11362
更新日期:2020-08-01 00:00:00
abstract::The cytokertatins in respiratory epithelial cells (REC) of human nasal polyps and turbinates were analyzed by immunohistochemistry. Cytokeratin 19 (CK19) was present in all REC, CK5 and 14 were expressed primarily in basal cells, and CK7, 8, and 18 were found in suprabasal cells. Differences in cytoplasmic locations w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1072
更新日期:1995-04-01 00:00:00
abstract::Grafting material for vaginal reconstruction commonly includes the bowel, peritoneum, skin, and amniotic membrane. Bone marrow mesenchymal stem cells (MSCs) have the potential of multilineage differentiation into a variety of cells and have been widely explored in tissue engineering. In the current study, we examined ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10495
更新日期:2015-11-01 00:00:00
abstract::Introns of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and evolutionary relationships through ILP (Intron Length Polymorphism). Here we show that the intron present within the 5'UTR sequence of some rice beta-tubulin genes can...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.11.013
更新日期:2008-05-01 00:00:00
abstract::Lead is a heavy metal of considerable environmental and occupational concern and there is growing evidence that it is toxic to the human immune system. In this regard, this study examined the effect of lead (Pb) exposure to peritoneal macrophages (Mvarphis) of mice (Mus musculus) cultivated in DMEM medium supplemented...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.03.010
更新日期:2006-07-01 00:00:00
abstract::Periodontitis is a chronic inflammatory disease that results in the destruction of periodontal soft tissue and the resorption of alveolar bone. Evidence indicates that in diabetic patients, hyperglycemia suppresses periodontal ligament stem cell (PDLSC) functions and leads to difficulties in periodontal repair. The pr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11445
更新日期:2020-12-01 00:00:00
abstract::Recently, we reported that human PDB (placental decidua basalis) is an excellent source of MSCs (mesenchymal stem cells), meanwhile, PDB-MSCs could survive under hypoxia and serum deprivation. Herein, we investigated the proliferation, clonogentic efficiency, phenotypes, metabolic activity and cytokines secretion of P...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090044
更新日期:2010-01-27 00:00:00
abstract::A method based on the measurement at room temperature of tryptophan phosphorescence (RTTP) gives the unique possibility to investigate the dynamic structure of membrane proteins without their isolation from cells. This method was used to study the influence of tert- butyl hydroperoxide (t -BHP) on Chinese hamster fibr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0366
更新日期:1999-01-01 00:00:00
abstract::The rates of intracellular protein degradation, of identically labelled populations of proteins, were compared in hepatocytes cultured at 37 degrees (on an adsorbed collagen layer) and in cells preserved on gelatin gels at 10 degrees C. The half-lives of the long-lived proteins were 35.4+/-8.6 h (N=4) and 692.9+/-216....
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0333
更新日期:1999-01-01 00:00:00
abstract::In spite of certain clinical limitations, such as teratoma formation, the use of stem cells is considered as an appropriate source in cell therapy and tissue engineering. This study shows human endometrial stem cells (hEnSCs) has exceptional differentiation ability in hepatocyte formation. hEnSCs have high purificatio...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10278
更新日期:2014-07-01 00:00:00
abstract::We previously reported that mechanical vibration-induced proinflammatory cytokines, interleukin-6 (IL-6) and IL-8, expression in human periodontal ligament (hPDL) cells, however, the underlying mechanism remained unclear. Mechanical stimuli are able to activate cellular responses by inducing the activation of several ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11267
更新日期:2020-02-01 00:00:00
abstract::Protein expression during the early development of Xenopus has been followed by 2D-polyacrylamide gel electrophoresis (PAGE). The analysis of two-dimensional maps of eggs and embryos at different stages of development has allowed the separation of more than 2000 spots. Identification of numerous polypeptides was obtai...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0296
更新日期:1998-01-01 00:00:00
abstract::The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the ga...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1102
更新日期:1994-07-01 00:00:00
abstract::The aim of this study was to obtain membrane-bound alkaline phosphatase from osteoblastic-like cells of human alveolar bone. Cells were obtained by enzymatic digestion and maintained in primary culture in osteogenic medium until subconfluence. First passage cells were cultured in the same medium and at 7, 14, and 21 d...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.06.002
更新日期:2007-11-01 00:00:00
abstract::This study examined whether Gd (gadolinium) could suppress prostate cancer cell migration and prostate cancer cell-induced osteoclast differentiation. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] and colony forming assay showed that GdCl3 treatment inhibited both cell viability and colony for...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100870
更新日期:2011-11-01 00:00:00
abstract::A structural link between cell's nucleus and centrosome was proposed years ago. Such a link was suggested to maintain nucleus-centrosome axis, determine polarity of interphase cells and ensure spindle assembly in mitotic cells. The idea of structural link is supported by the facts that centrosomes are usually located ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10016
更新日期:2013-02-01 00:00:00
abstract::Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which re...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0964
更新日期:2002-01-01 00:00:00
abstract::BPD (bronchopulmonary dysplasia) is predominantly characterized by persistent abnormalities in lung structure and arrested lung development, but therapy can be palliative. While promising, the use of BMSC (bone marrow-derived mesenchymal stem cell) in the treatment of lung diseases remains controversial. We have asses...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110447
更新日期:2012-06-01 00:00:00
abstract::To better treat patients with non-small cell lung cancer (NSCLC), the investigations on novel molecules affecting NSCLC progression are of vital importance. Long noncoding RNAs (lncRNAs) are identified as pivotal regulators that can affect the cellular activities of carcinomas. Long intergenic non-protein coding RNA 6...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11361
更新日期:2020-08-01 00:00:00
abstract::Wnt signalling regulates cell proliferation and cell fate determination during embryogenesis. However, little is known about the developmental role of one Wnt family member, Wnt-3, during avian development. To investigate the possible functions of Wnt-3, its expression pattern was determined using whole-mount in situ ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090133
更新日期:2010-07-01 00:00:00
abstract::The mdx mouse has been used as an animal model for human Duchenne muscular dystrophy (DMD). Unlike DMD, skeletal muscles of mdx mice undergo successful regeneration and do not show extensive fibrosis and functional impairment. Growth factors have been proposed to be involved in muscle growth and regeneration. We compa...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1068
更新日期:1994-04-01 00:00:00
abstract::Cell motility, cell migration and phagocytosis are distinct, though frequently sequential, processes. They are fundamental for the maintenance of homoeostasis in single cells as well as in pluricellular organisms. Like vertebrates, invertebrate immune functions are strictly dependent on cell motility, chemotaxis and p...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1042/CBI20100514
更新日期:2010-11-01 00:00:00
abstract::We have investigated the effect of visible light on animal cells using light-emitting diodes to emit monochromatic visible light (red, yellow, green and blue light). To explore the relevant mechanism of apoptosis, we assessed the intracellular superoxide anion production and mitochondrial membrane potential (ΔΨm) of B...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10069
更新日期:2013-06-01 00:00:00
abstract::Ricin is internalized after binding at the cell surface via lectin activity of the B-chain recognizing terminal galactose residues. Ricin-A chain is then translocated to the cytosol from various endocytic structures. Cell death is the result of catalytic inactivation of protein synthesis. Using (125)I-ricin overlays, ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0120
更新日期:1997-03-01 00:00:00
abstract::We studied the genotoxic potential of a carcinogen in the human hepatoma cell line, HepG2, and in primary rat hepatocyte culture. HepG2 is a well differentiated human hepatoblastoma cell line with biotransforming capacity. Rat hepatocytes were obtained by the standard two-step in situ perfusion technique. Following be...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1083
更新日期:1993-04-01 00:00:00
abstract::To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary ex...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.09.012
更新日期:2005-02-01 00:00:00