Abstract:
:Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on slide and have adapted this to our electrotaxis chamber, thereby offering a novel approach to quantify cellular responses to electric fields (EFs) in 3D conditions, in different matrices, with different strains of cells, under various field strengths. These developments used Dictyostelium cells to illustrate possible applications and limitations.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Zhao S,Gao R,Devreotes PN,Mogilner A,Zhao Mdoi
10.1002/cbin.10116subject
Has Abstractpub_date
2013-09-01 00:00:00pages
995-1002issue
9eissn
1065-6995issn
1095-8355journal_volume
37pub_type
杂志文章abstract::Almost universally, the effective stimulus for mammalian 9+0 primary cilia has been taken to be bending. In this article I point out that in several physiological contexts there is great advantage in detecting pressure differences across the cell wall, i.e. axially directed forces pushing fluid to and fro through the ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1016/j.cellbi.2008.03.001
更新日期:2008-04-01 00:00:00
abstract::As embryonic progenitors for the gametes, PGCs (primordial germ cells) proliferate and develop under strict regulation of numerous intrinsic and external factors. As the most active natural metabolite of vitamin A, all-trans RA (retinoic acid) plays pivotal roles in regulating development of various cells. The prolife...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110542
更新日期:2012-08-01 00:00:00
abstract::Tissue engineering of bone has been increasingly used in the bone defect repair. To generate osteoblasts is a major approach, and here we have examined ways of improving the efficiency of producing osteoblasts. Adipose stem cells (ADSC) were prepared from rat mesentery tissue, and transfected with Cbfa1 gene vector or...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10147
更新日期:2013-11-01 00:00:00
abstract::Tripartite motif protein 25 (TRIM25) expression was altered in various human cancers. Herein, we found that the expression of TRIM25 was elevated in hepatocellular carcinoma (HCC) tissues and cell lines. Knockdown of TRIM25 increased the sensitivity of HCC HepG2 cells to epirubicin (EPI), as indicated by reduced cell ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11346
更新日期:2020-07-01 00:00:00
abstract::The spatial distribution of acid membrane organelles and their relationships with normal and vacuolated transverse tubules has been studied in living frog skeletal muscle fibres using confocal microscopy. Acridine orange (AO) was used to evaluate acid compartments, while a lipophilic styryl dye, RH 414, was employed t...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.06.017
更新日期:2006-11-01 00:00:00
abstract::We analyzed whether the embryonic (E12.5-E14.5) mouse retina possesses genuine neural stem cells and how they respond to defined growth factors and extracellular matrix molecules. Whereas most combinations produced no or limited cell survival and proliferation in culture, FGF-2 plus heparin and laminin stimulated prol...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.11.031
更新日期:2007-07-01 00:00:00
abstract::In plastic and reconstructive field, it would be much beneficial to fabricate an engineered adipose tissue substitute allowing reliable and complete fat tissue regeneration. Tissue engineering chamber (TEC) holds the promise to optimize an adipogenic configuration that is efficacious as well as reproducible. A frequen...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10731
更新日期:2017-04-01 00:00:00
abstract::Neuroinflammatory disorders such as Alzheimer's and Parkinson's diseases are characterised by chronic inflammation and loss of vascular integrity. Bradykinin 1 receptor (B1R) activation has been implicated in many neuroinflammatory diseases, but the contribution of B1R to inflammation and vascular breakdown is yet to ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11232
更新日期:2019-09-09 00:00:00
abstract::Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) are attached to each other in the bone marrow (BM) cavity and in in vitro cultures, and this adhesion has important physiological significance. We demonstrated that cell proliferation could be promoted when MSCs were co-cultured with EPCs, which was...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10579
更新日期:2016-04-01 00:00:00
abstract::The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were search...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0099
更新日期:1996-11-01 00:00:00
abstract::Ca(2+)mobilization induced by ATP, isoproterenol and the Ca(2+)-ATPase inhibitor thapsigargin in the human submandibular duct cell line A253 was investigated using the Ca(2+)-sensitive fluorescent indicator fura-2. ATP and isoproterenol increased cytosolic free Ca(2+)([Ca(2+)](i)) and subsequent exposure to thapsigarg...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0503
更新日期:2000-01-01 00:00:00
abstract::To establish useful human normal cell lines, TERT (telomerase reverse transcriptase) cDNA was transfected into normal female lung fibroblast, TIG-1. After long-term-sub-cultivation of 74 individual clones selected for resistance to G418, we obtained 55 cultures with normal range of life span [75 PDL (population doubli...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110460
更新日期:2012-06-01 00:00:00
abstract::Disconnected interacting protein 2 (DIP2) is a highly conserved protein family among invertebrates and vertebrates, but its function remains unclear. In this paper, we summarized the conservation of gene sequences and protein domains of DIP2 family members and predicted that they may have a similar functional role in ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11106
更新日期:2019-04-01 00:00:00
abstract::CD25+Treg cells (CD4+CD25+Foxp3+ regulatory T cells) play a central role in the maintenance of peripheral self-tolerance and immune homoeostasis. A previous study showed that CD25+Treg cells suppressed the differentiation and function of Th1 cells in vivo and in vitro. However, the mechanism of suppressing Th1 cell di...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100528
更新日期:2011-07-01 00:00:00
abstract::MicroRNA-21 (miR-21) was reported to act as an oncogene during the development of many human tumors. However, little was revealed about the function of miR-21 in retinoblastoma (RB). In this study, we examined the expression of miR-21 in RB tissues and explored the relationship between miR-21 and phosphatase and tensi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10678
更新日期:2016-12-01 00:00:00
abstract::Metallothioneins (MTs) are thought to participate in a wide variety of physiological roles, but the mechanisms involved are still unclear. The study was designed to examine the possible factors related to these mechanisms. Methods, including transfection, MTT assay and flow cytometry, were used to investigate the effe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.05.008
更新日期:2005-10-01 00:00:00
abstract::An acetylcholine (ACh) agonist, carbachol (Cch), causes hepatocytes to proliferate in the presence of hepatic nonparenchymal cells (HNPCs). To identify the HNPCs and ACh receptor subtypes involved in carbachol-induced hepatocyte proliferation (CIHP), we examined two types of vascular cells as candidates for HNPCs medi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.01.018
更新日期:2009-04-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) exists low efficiency to trans-differentiate into other germinal layer cell types. One key issue is to discover the effect of important factor on MSCs differentiation abiltiy. In this study, we investigated the role and mechanism of epiregulin (EREG) on the osteogenic differentiation and ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11305
更新日期:2020-04-01 00:00:00
abstract::Gelatinase activity and inhibitory activity against collagenase were measured in serum-free medium conditioned by murine colonic carcinoma cells with different spontaneous metastatic potentials to the lung. The medium conditioned with poorly metastatic NM11 cells gave higher inhibitory activity than that conditioned w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1057
更新日期:1994-03-01 00:00:00
abstract::The diabetic milieu is believed to change the activity, or result in damage of podocytes-a key component of the glomerular filtration barrier and known to secrete matrix for glomerular basement membrane. This in turn contributes to diabetic nephropathy. However, how podocyte dysfunction is triggered in diabetic nephro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10363
更新日期:2015-02-01 00:00:00
abstract::vasa (vas)-related genes are members of the DEAD-box protein family and are expressed in the germ cells of many Metazoa. We cloned vasa-related genes (PpVLG, CpVLG) and other DEAD-box family related genes (PpDRH1, PpDRH2, CpDRH, AtDRHr) from the colonial parasitic rhizocephalan barnacle Polyascus polygenea, the non-co...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.09.012
更新日期:2007-02-01 00:00:00
abstract::The epidermal keratinocytes express two major pairs of keratin polypeptides. One pair (K5/K14) expressed specifically in basal generative compartment and the other (K1/K10) expressed specifically in the differentiating suprabasal compartment. The switch in the expression of the keratins from proliferating to different...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:
更新日期:1996-04-01 00:00:00
abstract::FITC-insulin binding to previously hormone-treated Tetrahymena was studied by flow cytometry and confocal microscopy. Hormones produced by Tetrahymena were chosen for study and the hormone concentrations were administered between 10(-6) and 10(-21)M for 30 min. Endorphin, serotonin and insulin significantly reduced th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.06.019
更新日期:2006-12-01 00:00:00
abstract::Corticotropin-releasing hormone, which is the predominant regulator of neuroendocrine responses to stress, attenuates inflammation through stimulation of glucocorticoid release. Enhanced corticotropin-releasing hormone expression has been detected in inflammatory cells of the vascular endothelium, where it acts as a l...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11188
更新日期:2019-05-29 00:00:00
abstract::Protein composition and functional activity of various polysome subpopulations isolated from Vicia faba L. leaves and Triticum aestivum L. and Hordeum vulgare L. seedlings were studied. Membrane- and cytoskeleton-bound polysomes were more active in the wheat germ cell-free translational system than free polysomes. Sev...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0517
更新日期:2000-01-01 00:00:00
abstract::Epigenetic silencing of cancer-related genes by abnormal methylation and the reversal of this process by DNA methylation inhibitors represents a promising strategy in cancer therapy. As DNA methylation affects gene expression and chromatin structure, we investigated the effects of novel DNMT (DNA methyltransferase) in...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110649
更新日期:2012-11-01 00:00:00
abstract::The influence of maternal nicotine exposure during pregnancy and lactation on the Type II cells of lung tissue of one day old neonatal rat pups was investigated. The results clearly show that maternal nicotine exposure resulted in an increase in the type II cell count in the lungs of the offspring. In addition the lam...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1075
更新日期:1995-04-01 00:00:00
abstract::Key to mitochondrial activities is the maintenance of mitochondrial morphology, specifically cristae structures formed by the invagination of the inner membrane that are enriched in proteins of the electron transport chain. In Saccharomyces cerevisiae , these cristae folds are a result of the membrane fusion activitie...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11219
更新日期:2019-08-23 00:00:00
abstract::Investigation of the relative slowness with which trypsin detaches epithelial cells from their adhesions with a substratum suggests that the adherent surface of the cells is normally rather inaccessible to the enzyme. Trypsinisation of cells through a porous filter allowing access of the enzyme to the underside of the...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1070
更新日期:1993-03-01 00:00:00
abstract::Bisphosphonates (BPs), especially zoledronic acid (ZOL), are clinically used to treat osteolytic bone lesions. However, serious side-effects may be also induced during the therapeutic process. To improve the BPs drugs, here, we investigated the effects of a series of ZOL derivatives with increasing number of methylene...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11546
更新日期:2021-01-06 00:00:00