Abstract:
:Investigation of the relative slowness with which trypsin detaches epithelial cells from their adhesions with a substratum suggests that the adherent surface of the cells is normally rather inaccessible to the enzyme. Trypsinisation of cells through a porous filter allowing access of the enzyme to the underside of the cells greatly accelerated detachment: a result consistent with this explanation.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Simpson K,McGrath M,Curtis Adoi
10.1006/cbir.1993.1070subject
Has Abstractpub_date
1993-03-01 00:00:00pages
341-8issue
3eissn
1065-6995issn
1095-8355pii
S1065-6995(83)71070-Xjournal_volume
17pub_type
杂志文章abstract::The synthesis of proteins from salt-tolerant Brassica oleracea L. var. botrytis L. subvar. cauliflora (Gars.) DC. (cauliflower) cell cultures is modified in relation to controls in several features. There are nine newly induced polypeptides in tolerant cultures (absent in control conditions). Some of them are only pre...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1146
更新日期:1993-09-01 00:00:00
abstract::Our study aimed to explore the function of cystic fibrosis transmembrane conductance regulator (CFTR) in esophageal cancer. Twenty patients with esophageal squamous cell carcinoma (ESCC) and 20 patients with esophageal adenocarcinoma (EA) were enrolled in this study. The levels of CFTR and NF-κB in tumor tissues and a...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11069
更新日期:2018-12-01 00:00:00
abstract::Introns of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and evolutionary relationships through ILP (Intron Length Polymorphism). Here we show that the intron present within the 5'UTR sequence of some rice beta-tubulin genes can...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.11.013
更新日期:2008-05-01 00:00:00
abstract::Accurate relative gene expression analysis by reverse transcription-quantitative polymerase chain reaction relies on the usage of suitable reference genes for data normalization. The RNA content of small extracellular vesicles including exosomes is growingly considered as cancer biomarkers. So, reliable relative quant...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11556
更新日期:2021-01-27 00:00:00
abstract::Calmodulin-like skin protein (CLSP) is a secreted peptide that is produced by skin keratinocytes and some related epithelial cells. It has previously been shown that CLSP is recruited via the bloodstream into the central nervous system where it likely exerts a neuroprotective effect against toxicity related to Alzheim...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11159
更新日期:2019-07-01 00:00:00
abstract::DCs (dendritic cells) are the strongest professional APCs (antigen-presenting cells) to initiate immune responses against pathogens, but they are usually incompetent in initiating efficient immune responses in the progress of solid tumours. We have shown that Notch signalling plays a pivotal role in DC-dependent anti-...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100462
更新日期:2012-03-01 00:00:00
abstract::The size and number of secretory granules in late larval salivary glands of Drosophila melanogaster have been related to interecdysial and early metamorphic development represented by well-known puffs in polytene chromosomes. Interecdysial period (puff stage 1 (PS1)) is characterized by presence of numerous small gran...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0433
更新日期:1999-01-01 00:00:00
abstract::Increasing potassium chloride concentration from 0 to 100 mM and magnesium chloride from 0 to 2 mM show a parallel rate increase in polymerizing actin, whereas increasing calcium chloride concentration from 0 to 0.2 mM decreases the rate of polymerizing actin. The presence of alpha-actinin has little influence on the ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0898
更新日期:2002-01-01 00:00:00
abstract::Serine/threonine protein phosphatase 2A (PP2A) is involved in regulating various physiological processes including cell cycle, growth, apoptosis, and signal transduction. Osteoblast differentiation is controlled by main bone specific transcription factors including Osterix, distal-less homeobox 5 (Dlx5), and Runt-rela...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10902
更新日期:2018-04-01 00:00:00
abstract::Steroid hormones and their receptors play pivotal roles in avian reproduction and development. 17β-estradiol (E2 ) is an essential mediator in ovarian development. In this study, Day 3 (D3) chicks were injected with E2 (0.025, 0.25 or 2.5 mg/kg body weight) for 4 days to assess the effects of estrogen on avian follicl...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10766
更新日期:2017-06-01 00:00:00
abstract::To identify the molecular basis by which nonpermissive temperature (NPT) induces cell differentiation in Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen, we performed global scale microarray and computational gene network analyses. In TTE3 cells, inactivation of the large T-antigen by a NPT at ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.04.007
更新日期:2007-10-01 00:00:00
abstract::The Kv2.1 potassium channel is a principal component of the delayed rectifier I(K) current in the pyramidal neurons of cortex and hippocampus. We used whole-cell patch-clamp recording techniques to systemically compare the electrophysiological properties between the native neuronal I(K) current of cultured rat hippoca...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.08.019
更新日期:2008-12-01 00:00:00
abstract::The cytosolic lipid droplets (cLDs) store excess intracellular lipids, and perilipin-2 is believed to protect cLDs from degradation. Here, we investigated the role of the small G-protein Arf1 and the proteasome in the fates of perilipin-2 and cLDs. In oleate-loaded cells, upon brefeldin A (BFA) treatment, perilipin-2 ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10547
更新日期:2016-02-01 00:00:00
abstract::MicroRNAs (miRNAs) play an increasingly important role in cancer growth by coordinately suppressing genes that control cell migration, proliferation, and invasion. The above results can be achieved through the regulation of gene expression by miRNAs by suppressing translation or the direct sequence-specific degradatio...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10754
更新日期:2017-12-01 00:00:00
abstract::Mouse trisomy 16 (Ts16) appears to provide an animal model of Down's syndrome in that a portion of mouse chromosome 16 is syntenic with part of human chromosome 21. Trisomy 21 in human beings leads to the mental retardation of Down's syndrome and in middle age, to some presenile anatomic and clinical features of Alzhe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0089
更新日期:1996-10-01 00:00:00
abstract::Conditioned medium (CM) (cell secretome) is a cocktail of growth factors, cytokines, and other soluble mediators secreted by cells into a culture medium. These growth factors are fundamental in many cellular processes such as cell growth, differentiation, and others and the composition of these factors is individual f...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11317
更新日期:2020-05-01 00:00:00
abstract::The influence of maternal nicotine exposure during pregnancy and lactation on the Type II cells of lung tissue of one day old neonatal rat pups was investigated. The results clearly show that maternal nicotine exposure resulted in an increase in the type II cell count in the lungs of the offspring. In addition the lam...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1075
更新日期:1995-04-01 00:00:00
abstract::The purpose of this study was three fold: (1) to reveal the implications of Wnt5A for cytokine and chemokine production by human ovarian cancer cell line SKOV-3 cells, (2) to determine the influence of Wnt5A on chemotactic SKOV-3 cell migration, and (3) to assess the effect of inflammatory mediators on Wnt5A expressio...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10551
更新日期:2016-02-01 00:00:00
abstract::Genistein is an isoflavone that has estrogen (E2 )-like activity and is beneficial for follicular development, but little is known regarding its function in oxidative stress (OS)-mediated granulosa cell (GC) injury. Here, we found that after exposure to H2 O2 , Genistein weakened the elevated levels of intracellular r...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11244
更新日期:2020-02-01 00:00:00
abstract::As embryonic progenitors for the gametes, PGCs (primordial germ cells) proliferate and develop under strict regulation of numerous intrinsic and external factors. As the most active natural metabolite of vitamin A, all-trans RA (retinoic acid) plays pivotal roles in regulating development of various cells. The prolife...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110542
更新日期:2012-08-01 00:00:00
abstract::CLIC5 (chloride intracellular channel 5) is a CLIC (chloride intracellular channel) with various functions. Its high expression in skeletal muscle and association with actin-based cytoskeleton suggests that it may play an important role in muscle tissue. This study was conducted to examine whether CLIC5 regulates the ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090334
更新日期:2010-03-08 00:00:00
abstract::The pharmacological modulation of collagen turnover is a strategy potentially useful in different skin conditions. The serine protease inhibitor Serpin A1 and portions of its C-terminal region have been investigated as collagen modulators. To clarify the mechanisms by which the C-terminal 409-418 peptide SA1-III incre...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11018
更新日期:2018-09-01 00:00:00
abstract::The intranucleolar chromatin has been detected by feeding HeLa cells with bromodeoxyuridine and then using an immunocytochemical technique for its detection at the ultrastructural level. Labelling was mainly observed on the dense fibrillar component (DFC), which surrounds the fibrillar centres (FC). Labelling could al...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0265
更新日期:1998-01-01 00:00:00
abstract::Coumarin and its derivative 7-hydroxycoumarin (7-OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl-2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu-1. The cells were cultured in vitro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.04.003
更新日期:2005-08-01 00:00:00
abstract::Our aim has been to elucidate the possible mechanism of CJX1, an amlodipine derivative, in the modulation of P-gp function by determining its effect on P-gp ATPase activity. Basal P-gp ATPase activity was increased by CJX1 with half-maximal activity concentration (Km) of 8.6+/-1.4 microM. Kinetic analysis indicated a ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.06.023
更新日期:2009-10-01 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is a common cancer with poor prognosis and high mortality. The role of CCN5 has attracted a great focus on the regulation of cancer progression. However, the biological function and mechanism of CCN5 in OSCC are still not well elucidated. The current study was designed to determine ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11296
更新日期:2020-04-01 00:00:00
abstract::Primary cilium development along with other components of the centrosome in mammalian cells was analysed ultrastructurally and by immunofluorescent staining with anti-acetylated tubulin antibodies. We categorized two types of primary cilia, nascent cilia that are about 1microm long located inside the cytoplasm, and tr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2003.11.013
更新日期:2004-01-01 00:00:00
abstract::Pig endothelial cells are the first cells to interact with human immune components after organ xenotransplantation, which is a procedure currently considered to be the best treatment option for end-stage organ failure. It is, therefore, essential to study the mechanisms of molecular interaction between pig endothelial...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.03.016
更新日期:2005-08-01 00:00:00
abstract::microRNAs (miRNAs), through negatively regulating their target genes, influence the development and progression of many cancers. Previously, we found miR-483 was overexpressed in esophageal squamous cell carcinoma (ESCC) tissues, and its overexpression was negatively correlated with the prognosis and positively correl...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10585
更新日期:2016-04-01 00:00:00
abstract::Immunocytochemical staining of citrulline-containing proteins, i.e. products of endogenous peptidylarginine deiminase (EC 3.5.3.15) reaction, has been performed by chemical modification of citrulline residues in situ, followed by probing with IgG specific to the modified residues. Rat spinal cords that had been preinc...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1094
更新日期:1993-05-01 00:00:00