Abstract:
:Neuroinflammatory disorders such as Alzheimer's and Parkinson's diseases are characterised by chronic inflammation and loss of vascular integrity. Bradykinin 1 receptor (B1R) activation has been implicated in many neuroinflammatory diseases, but the contribution of B1R to inflammation and vascular breakdown is yet to be determined. As a result, the present study evaluated the effect of B1R stimulation using Des-Arg-9-BK on the cytokine profile and junctional properties of human cerebral microvascular endothelial cells (hCMVECs). Results showed that stimulation of B1R receptors increased secretion of pro-inflammatory cytokines, interleukin-6 (IL-6), IL-8, intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and monocyte chemoattractant protein-1 (MCP-1), but decreased the expression of vascular endothelial growth factor (VEGF), a cytokine and growth factor required for maintenance of the vasculature. B1R stimulation also resulted in the loss of occludin expression at tight junctions with no change in VE-cadherin expression. There was also a significant increase in permeability to Evans blue albumin, suggesting an increase of vascular permeability. Taken together, these results suggest that B1R activation that occurs in neuroinflammatory diseases may contribute to both the inflammation and loss of blood-brain barrier integrity that is characteristic of these diseases.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Mugisho OO,Robilliard LD,Nicholson LFB,Graham ES,O'Carroll SJdoi
10.1002/cbin.11232subject
Has Abstractpub_date
2019-09-09 00:00:00eissn
1065-6995issn
1095-8355pub_type
杂志文章abstract::Zinc-finger E-box binding homeobox 1 (ZEB1) is a master regulator of epithelial-mesenchymal transition (EMT) and has been implicated in primary epithelial cancer biological processes, such as invasion and metastasis. However, the role of ZEB1 in progression of melanoma and cancer stem cells (CSCs) remains obscure. In ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10223
更新日期:2014-04-01 00:00:00
abstract::Phosphoserine has potential effectiveness as a simple substrate in preparing bone replacement materials, which could enhance bone forming ability. However, there is a need to investigate the independent effect of phosphoserine on osteogenic differentiation of human adipose stem cells (hADSCs). hADSCs were cultured in ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10203
更新日期:2014-03-01 00:00:00
abstract::Type I diabetes is characterized by the deficiency of endocrine beta cells in the pancreatic islets of Langerhans and transplantation of islet cells can be an effective therapeutic approach. Embryonic stem cells can be differentiated into any cell type, and therefore represent an unlimited source of islet cells for th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.12.017
更新日期:2008-04-01 00:00:00
abstract::The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were search...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0099
更新日期:1996-11-01 00:00:00
abstract::We have investigated oxidized low-density lipoprotein (ox-LDL) induced senescence in hematopoietic stem cells (HCs). Mouse Sca-1+ HCs were separated and purified using the magnetic activated cell sorting technique. Ox-LDL induced significant senescence in HCs measured by SA-β-Gal staining, and reduced CFU-Mix colony-f...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10121
更新日期:2013-09-01 00:00:00
abstract::Prolapsus uteri in pelvic supportive disorders are common in elderly women, and their etiology remains unclear. We examined elastin-binding proteins (EBPs) and binding sites in cultured cardinal ligament fibroblasts derived from elderly patients with prolapsus uteri (HPLiF) and compared them with those from age-matche...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0877
更新日期:2002-01-01 00:00:00
abstract::Lead is a heavy metal of considerable environmental and occupational concern and there is growing evidence that it is toxic to the human immune system. In this regard, this study examined the effect of lead (Pb) exposure to peritoneal macrophages (Mvarphis) of mice (Mus musculus) cultivated in DMEM medium supplemented...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.03.010
更新日期:2006-07-01 00:00:00
abstract::Mouse trisomy 16 (Ts16) appears to provide an animal model of Down's syndrome in that a portion of mouse chromosome 16 is syntenic with part of human chromosome 21. Trisomy 21 in human beings leads to the mental retardation of Down's syndrome and in middle age, to some presenile anatomic and clinical features of Alzhe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0089
更新日期:1996-10-01 00:00:00
abstract::It has been shown in many investigations that the abnormally increasing production and deposition of collagen is one of the important mechanisms of pathological scars and other fibrotic diseases [Wang Z, Inokuchi T, Nemoto TK, Uehara M, Baba TT. Antisense oligonucleotide against collagen-specific molecular chaperone 4...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.10.009
更新日期:2008-05-01 00:00:00
abstract::A view is emerging that human amylin (HA) kills pancreatic islet beta-cells by apoptosis. This study strengthens this view by documenting time-dependent morphological and ultrastructural changes in 10 microm HA-treated cultured RINm5F islet beta-cells. Membrane blebbing and microvilli loss were the earliest detectable...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0643
更新日期:2001-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) are attached to each other in the bone marrow (BM) cavity and in in vitro cultures, and this adhesion has important physiological significance. We demonstrated that cell proliferation could be promoted when MSCs were co-cultured with EPCs, which was...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10579
更新日期:2016-04-01 00:00:00
abstract::Cytoplasmic proteins with binding capability to AU-rich instability determinant sequences (ARE) of tumour necrosis factor alpha (TNFalpha) mRNA 3' untranslated region (3'UTR) were assessed in human lymphoid cells. In vitro label transfer experiments using wild type as well as mutant sequences in which the 70 nucleotid...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0418
更新日期:1999-01-01 00:00:00
abstract::To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary ex...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.09.012
更新日期:2005-02-01 00:00:00
abstract::A convenient means of measuring apoptosis is described using an automated analyzer (MF-1; Sysmex), which normally provides rapid measurement of malarial parasites. By means of this MF-1 procedure, apoptotic cells exhibited characteristic changes of light scatter (size) and fluorescence (DNA content) relating to cell s...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0560
更新日期:2000-01-01 00:00:00
abstract::The mitogen activated protein kinases (MAPK) signaling cascade plays an important role in cell life. We proved that small interfering RNAs targeting MAPK1 (siRNA-2) could inhibit HeLa cell growth, but the effects of siRNA-2 on gene expression profile were unclear. Using Affymetrix GeneChip HG-U133A 2.0, we identified ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.019
更新日期:2008-09-01 00:00:00
abstract::Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which re...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0964
更新日期:2002-01-01 00:00:00
abstract::Coumarin and its derivative 7-hydroxycoumarin (7-OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl-2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu-1. The cells were cultured in vitro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.04.003
更新日期:2005-08-01 00:00:00
abstract::While much evidence indicates a high degree of spatial organization in the nucleus, the underlying molecular structures that support it remain poorly characterized. By extracting with high concentrations of RNase A in a modification of the sequential extraction protocol of Penman, we have identified a novel intranucle...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1997.0149
更新日期:1997-06-01 00:00:00
abstract::Conventional kinesin is an ATPase-active motor protein that is involved in the microtubule-dependent transport of cytoplasmic cargoes. The present paper provides a condensed overview on kinesin-1 and its motility generation outside a living cell. Moreover, it demonstrates that the neuron-specific kinesin KIF5A enables...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1016/j.cellbi.2007.12.001
更新日期:2008-05-01 00:00:00
abstract::A significant decrease in tissue pH or acidosis is a common feature of numerous diseases, including RA (rheumatoid arthritis). Cartilage homoeostasis is profoundly affected by local acidosis in the joints. The diuretic, amiloride, is neuroprotective in models of cerebral ischaemia, a property attributable to the inhib...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110432
更新日期:2012-07-01 00:00:00
abstract::Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of topography. Whilst it has be known for some time that cells can be guided by micro-topography, it is only recently beco...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2003.12.004
更新日期:2004-01-01 00:00:00
abstract::Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10116
更新日期:2013-09-01 00:00:00
abstract::Hyperglycemia is one of the most important pathogenesis of diabetic osteopathy. Several lines of studies indicate Runx2 plays a critical role in the process of osteogenic differentiation. However, little studies have analyzed the effect of Runx2 on osteoblast differentiation of rat bone mesenchymal stem cells (rBMSCs)...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10779
更新日期:2017-08-01 00:00:00
abstract::This study provides evidence that, in the soluble fraction from buffer-washed pea thylakoids, one form of soluble carbonic anhydrase (CA) is associated with rubisco in a stromal protein complex. On native-PAGE gels, it is present as a protein band with MW approximately 160 kDa. On SDS-PAGE gels, it is resolved as a si...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.01.010
更新日期:2008-06-01 00:00:00
abstract::The objective of this study was to test the hypothesis that cytoskeletal actin fragmentation is mediated through caspase-2, specifically examining the ability of a caspase-2 inhibitor to interfere with actin fragmentation, in comparison with a caspase-3 inhibitor. Cardiomyocytes were cultured from embryonic chick hear...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.07.012
更新日期:2004-01-01 00:00:00
abstract::Rb/E2F regulates many genes that encode proteins required for the cell cycle. Using affymetrix microarrays we previously identified genes regulated by the Rb proteins p130 and p107, many of which are involved in the cell cycle. Several genes with unknown functions were also repressed by p130 and p107, of which some ha...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.06.013
更新日期:2007-12-01 00:00:00
abstract::Adult peripheral blood-derived cells are able to differentiate into a variety of cell types, including nerve cells, liver-like cells and epithelial cells. However, their differentiation into retina-like cells is controversial. In the present study, transdifferentiation potential of human adult peripheral blood mononuc...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100146
更新日期:2011-06-01 00:00:00
abstract::Almost universally, the effective stimulus for mammalian 9+0 primary cilia has been taken to be bending. In this article I point out that in several physiological contexts there is great advantage in detecting pressure differences across the cell wall, i.e. axially directed forces pushing fluid to and fro through the ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1016/j.cellbi.2008.03.001
更新日期:2008-04-01 00:00:00
abstract::Key to mitochondrial activities is the maintenance of mitochondrial morphology, specifically cristae structures formed by the invagination of the inner membrane that are enriched in proteins of the electron transport chain. In Saccharomyces cerevisiae , these cristae folds are a result of the membrane fusion activitie...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11219
更新日期:2019-08-23 00:00:00
abstract::Neuroinflammation is closely related with the pathogenesis and progress of neurodegenerative diseases including Alzheimer's disease (AD). Loganin, an iridoid glycoside obtained from traditional Chinese medicine Cornus officinalis, has properties of inhibiting inflammation and improving memory. The present study was ai...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11060
更新日期:2018-12-01 00:00:00