A 160 kDa protein with carbonic anhydrase activity is complexed with rubisco on the outer surface of thylakoids.

Abstract:

:This study provides evidence that, in the soluble fraction from buffer-washed pea thylakoids, one form of soluble carbonic anhydrase (CA) is associated with rubisco in a stromal protein complex. On native-PAGE gels, it is present as a protein band with MW approximately 160 kDa. On SDS-PAGE gels, it is resolved as a single 25-kDa polypeptide. Analysis of Western blots developed with polyclonal antibodies to barley rubisco and to soluble pea CA shows that a 160-kDa protein with CA activity is associated with rubisco in a protein complex localized on the outer surface of thylakoid membranes.

journal_name

Cell Biol Int

authors

Lazova GN,Stemler AJ

doi

10.1016/j.cellbi.2008.01.010

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

646-53

issue

6

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(08)00029-2

journal_volume

32

pub_type

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