Rab11 is essential for fertility in Drosophila.

Abstract:

:Rab11, a small GTP binding protein involved in vesicular trafficking, has emerged as a key player in regulating various cellular events during Drosophila development and differentiation. In our earlier study a P-insertion line, Rab11mo, was established as a new hypomorphic allele of Rab11 gene, showing degenerated eye phenotype, bristle abnormalities and sterility. We show here that Rab11 is expressed in the entire testis, more prominently in the secretory cells, and in ovary it is localized at the posterior pole. Rab11mo males and females are sterile. The sterility in males has been attributed to defects in the sperm individualization process, while in females, cytoskeleton disruption and reduction/loss of the posteriorly localized protein, Vasa, as a consequence of loss/mislocalization of Rab11 might be the cause of sterility. Fertility as well as the posterior localization of Rab11 and Vasa or cytoskeleton integrity was restored in pCaSpeR4-Rab11/+; Rab11mo/Rab11mo egg chambers, confirming the requirement of Rab11 in these events.

journal_name

Cell Biol Int

authors

Tiwari AK,Alone DP,Roy JK

doi

10.1016/j.cellbi.2008.04.002

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

1158-68

issue

9

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(08)00389-2

journal_volume

32

pub_type

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