The effect of insulin on proteins associated with free, cytoskeletal-bound and membrane-bound polysome populations.

Abstract:

:It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal-bound (CBP) and membrane-bound polysome (MBP) populations. This paper concerns an analysis of the pattern of proteins in high-salt extracts of FP, CBP and MBP isolated from Krebs II ascites and MPC-11 cells. A combined detergent/salt extraction procedure was used to isolate the three fractions of polysomes from control cells and from cells following short-term stimulation with insulin. There were differences in the protein patterns in the individual fractions and changes occurred after insulin stimulation.

journal_name

Cell Biol Int

authors

Moss R,Pryme IF,Vedeler A

doi

10.1006/cbir.1993.1039

subject

Has Abstract

pub_date

1993-12-01 00:00:00

pages

1065-73

issue

12

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(83)71039-5

journal_volume

17

pub_type

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