Abstract:
:It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal-bound (CBP) and membrane-bound polysome (MBP) populations. This paper concerns an analysis of the pattern of proteins in high-salt extracts of FP, CBP and MBP isolated from Krebs II ascites and MPC-11 cells. A combined detergent/salt extraction procedure was used to isolate the three fractions of polysomes from control cells and from cells following short-term stimulation with insulin. There were differences in the protein patterns in the individual fractions and changes occurred after insulin stimulation.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Moss R,Pryme IF,Vedeler Adoi
10.1006/cbir.1993.1039subject
Has Abstractpub_date
1993-12-01 00:00:00pages
1065-73issue
12eissn
1065-6995issn
1095-8355pii
S1065-6995(83)71039-5journal_volume
17pub_type
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