Abstract:
:Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72,900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the underlying mechanisms. Effects of AP on cell viability, proliferation, apoptosis, migration, invasion, and expressions of long noncoding RNA H19 (lncRNA-H19), microRNA (miR)-675, and CD44 were assessed. Then, effects of miR-675 overexpression on AP-treated cells were analyzed. Next, expression of key kinases in the PI3K/AKT and JAK/ STAT pathways was detected. The possible target gene of miR-675 was finally explored. Cell viability was reduced by 200-500 µg/mL AP. Meanwhile, AP repressed cell proliferation, migration, and invasion, but induced apoptosis. Expressions of lncRNA-H19 and miR-675 were upregulated in neuroblastoma cells, and were downregulated by AP. AP was also identified to upregulate CD44. We next found AP affected SH-SY5Y cells through downregulating miR-675. Key kinases in the PI3K/AKT and JAK/STAT pathways were downregulated by AP stimulation, while these downregulations were abrogated by miR-675 overexpression. KIF1B isoform β (KIF1Bβ) is proved to be a target of miR-675. In conclusion, AP was first identified to inhibit proliferation, migration, and invasion but induce apoptosis. Furthermore, AP might repress tumorigenesis of SH-SY5Y cells through miR-675-mediated inactivation of the PI3K/AKT and JAK/STAT pathways. Besides, KIF1Bβ might be a target of miR-675.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Yang J,Shao X,Jiang J,Sun Y,Wang L,Sun Ldoi
10.1002/cbin.10954subject
Has Abstractpub_date
2018-07-01 00:00:00pages
867-876issue
7eissn
1065-6995issn
1095-8355journal_volume
42pub_type
杂志文章abstract::IPF is characterized by fibroblast accumulation, collagen deposition, and ECM remodeling, with myofibroblasts believed to be the effector cell type. Myofibroblasts develop due to EMT of lung alveolar epithelial cells, which can be induced by TGF-β. M2 macrophages, a macrophage subpopulation, secrete large amounts of T...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10788
更新日期:2017-09-01 00:00:00
abstract::Bcl-2 family proteins play a critical role in the regulation of apoptosis. Treatment of a human hepatocellular carcinoma cell line, QGY-7703, with Taxol induced apoptosis and Bcl-2 protein phosphorylation. Microscopic observation indicated that apoptotic bodies (0-15%) of Taxol-treated QGY cells appeared after 12 h of...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0619
更新日期:2001-01-01 00:00:00
abstract::Adaptation of cardiomyocytes to chronic hypoxia in cyanotic patients remains unclear. Mitochondrial biogenesis is enhanced in myocardium from cyanotic patients, which is possibly an adaptive response. Erythropoietin (EPO) in blood and its receptor (EPOR) on cardiomyocytes are upregulated by chronic hypoxia, suggesting...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10205
更新日期:2014-03-01 00:00:00
abstract::The mitogenic effect of TGF-alpha, acidic-FGF, basic-FGF and lithium on normal human breast epithelial cells was studied in a collagen gel culture system using a serum-free 1:1 mixture of Ham's F12 and DME medium containing insulin, cholera toxin and bovine serum albumin. TGF-alpha elicited a strong mitogenic response...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1077
更新日期:1993-04-01 00:00:00
abstract::CO25 cells, a mouse myoblast line, contain multiple centrioles and primary cilia. A most unusual feature has been the finding of large numbers of separate structures in single cells-up to a maximum of nine centrioles, six primary cilia, and 12 of both organelles together. Aberrant multipolar spindles were occasionally...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0103
更新日期:1996-12-01 00:00:00
abstract::In spite of certain clinical limitations, such as teratoma formation, the use of stem cells is considered as an appropriate source in cell therapy and tissue engineering. This study shows human endometrial stem cells (hEnSCs) has exceptional differentiation ability in hepatocyte formation. hEnSCs have high purificatio...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10278
更新日期:2014-07-01 00:00:00
abstract::The focal adhesion protein vinculin has been implicated in associating with soluble and membranous phospholipids. Detailed investigations over the past ten years describe the intermolecular interactions of the vinculin tail domain with soluble and membrane phospholipids. Previous studies have implied that the tail's u...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10978
更新日期:2018-08-01 00:00:00
abstract::Investigation of the relative slowness with which trypsin detaches epithelial cells from their adhesions with a substratum suggests that the adherent surface of the cells is normally rather inaccessible to the enzyme. Trypsinisation of cells through a porous filter allowing access of the enzyme to the underside of the...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1070
更新日期:1993-03-01 00:00:00
abstract::ES (embryonic stem)-derived cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES-derived cells to improve functional outcome following partially damaged breast and also the modification of mammary tissue to produce costly pro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090453
更新日期:2010-10-01 00:00:00
abstract::Inostamycin, which was recently isolated from Streptomyces sp. MH816-AF15 as an inhibitor of cytidine 5'-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG): inositol transferase, caused a G1-phase accumulation in the cell cycle of small cell lung carcinomas. To investigate whether the cytostatic effect of inostamycin is rest...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0706
更新日期:2001-01-01 00:00:00
abstract::Cell proliferation rates of diagnostic marrow aspirate cells of 21 children with Acute Lymphoblastic Leukaemia and 16 controls were compared using immunocytochemical labelling of PCNA and Ki-67 antigen as assessed by Confocal Laser Scanning Microscopy. The results showed an unexpected, highly significant degree of dis...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1123
更新日期:1994-09-01 00:00:00
abstract::Expression of the canine 180-kDa ribosome receptor p180 in yeast induces the synthesis of RER, and increases the mRNAs of secretory pathway proteins, and protein secretion. To assess whether p180 is a master regulator of cell secretion in mammalian cells, we stably expressed red fluorescent forms of the human p180 var...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.10.002
更新日期:2008-05-01 00:00:00
abstract::Calcium loss and muscle atrophy are two of the main metabolic changes experienced by astronauts and crew members during exposure to microgravity in space. Calcium and cytoskeletal events were investigated within sea urchin embryos which were cultured in space under both microgravity and 1 g conditions. Embryos were fi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0371
更新日期:1999-01-01 00:00:00
abstract::Hematopoietic stem and progenitor cells (HSPCs) are attractive targets in regenerative medicine, although the differences in their homeostatic maintenance between sexes along time are still under debate. We accurately monitored hematopoietic stem cells (HSCs), common lymphoid progenitors (CLPs), and common myeloid pro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10865
更新日期:2017-12-01 00:00:00
abstract::GDNF plays an important role in the survival and differentiation of primary dopaminergic neurons, but it requires multiple factors for its entire range of activities. This study investigated the effects of GDNF and its cofactors on the development of bFGF-responsive neural progenitor cells (NPCs), mesencephalic and co...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.01.006
更新日期:2004-01-01 00:00:00
abstract::Neuroblastoma is one of the most common solid tumours in children (8-10% of all malignancies). Over 22% of cases have N-myc amplification associated with aggressively growing neuroblastomas. Oncogene-induced sensitization of cells to apoptosis is an important mechanism for suppression of tumorigenesis. Tumour suppress...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110231
更新日期:2012-04-01 00:00:00
abstract::In order to investigate the effects of human yolk sac-derived endothelial cells (hYSECs) on the expansion of human hematopoietic stem/progenitor cells (HS/PCs) from umbilical cord blood (UCB) in vitro, we purified hYSEC-like cells from 4-5 week human yolk sacs, which were morphologically similar to endothelial cells a...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.06.006
更新日期:2006-11-01 00:00:00
abstract::At the end of transcardial perfusions with ice-cold physiological saline for 30 min or with isoosmotic potassium chloride for 5 min, but immediately before perfusion fixation, condenser-discharge electric shocks were administered to rats through surface electrodes pressed onto the temporal muscles of the scalped skull...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.11.002
更新日期:2006-02-01 00:00:00
abstract::An experimental model of leg lengthening was used to study the morphology of, the collagenous proteins present, and the collagen genes expressed in the regenerating tissue following 20% lengthening at four different distraction rates. At a distraction rate of 0.3 mm/day (8 weeks distraction), the regenerate consists o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0449
更新日期:2000-01-01 00:00:00
abstract::Many disturbances in electrolyte and hormonal balance in the body induced by functional impairment of renal parenchyma may affect the activity of amine precursor uptake and decarboxylation (APUD) cells, which constitute a very important link in the regulation of homeostasis. The aim of the present study was the morpho...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.03.012
更新日期:2004-01-01 00:00:00
abstract::Most hepatomas have a defect in prothrombin carboxylation, and can secrete under-carboxylated prothrombin or des-gamma-carboxy-prothrombin (DCP), the function of which is unknown. We considered that the prothrombin-DCP axis might also be involved in growth control. Hepatocytes and hepatoma cells were treated with prot...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.03.001
更新日期:2007-09-01 00:00:00
abstract::Grafting material for vaginal reconstruction commonly includes the bowel, peritoneum, skin, and amniotic membrane. Bone marrow mesenchymal stem cells (MSCs) have the potential of multilineage differentiation into a variety of cells and have been widely explored in tissue engineering. In the current study, we examined ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10495
更新日期:2015-11-01 00:00:00
abstract::The efficacies of chemotherapeutic agents are often limited by side effects and acquired drug resistance. We have investigated whether the differential expression pattern of 14-3-3σ affects cisplatin response in non-small cell lung cancer cell lines. Two pairs of parental/cisplatin resistant cell lines (A549/CRA549 an...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10006
更新日期:2013-01-01 00:00:00
abstract::Wnt signalling regulates cell proliferation and cell fate determination during embryogenesis. However, little is known about the developmental role of one Wnt family member, Wnt-3, during avian development. To investigate the possible functions of Wnt-3, its expression pattern was determined using whole-mount in situ ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090133
更新日期:2010-07-01 00:00:00
abstract::A subcellular fractionation procedure was developed to isolate the different endosomal compartments present during reticulocyte maturation. After reticulocyte lysis and removal of excess haemoglobin by gel chromatography, membrane vesicles were separated over a discontinuous sucrose gradient (10-40%). Two fractions we...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0917
更新日期:2002-01-01 00:00:00
abstract::Transplanted tumours could modify the intensity and temporal distribution of the cellular proliferation in normal cell populations, and partial hepatectomy alters the serum concentrations of substances involved in cellular proliferation, leading to the compensatory liver hyperplasia. The following experiments were des...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090282
更新日期:2010-02-05 00:00:00
abstract::Transforming growth factor β1 (TGFβ1) is a negative regulator of hematopoiesis, and yet, it is frequently found at the active sites of hematopoiesis. Here, we show for the first time that bone marrow-derived mononuclear cells (BM MNCs) secrete TGFβ1 in response to erythropoietin (EPO). We further show that human bone ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11437
更新日期:2020-11-01 00:00:00
abstract::ZO-1 is a peripheral protein that plays a central role in the macromolecular assembly of tight junctions by interacting with integral proteins (occludin, claudins, JAMs) of the membrane of adjoining cells, with the actin cytoskeleton, and with nuclear factors. Human ZO-1 is expressed in all epithelia and some speciali...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090067
更新日期:2010-05-07 00:00:00
abstract::We describe a method to hold living cells in place that ordinarily do not adhere to glass coverslips. The method, developed for insect spermatocytes but with application to other cell types, consists of embedding cells in a fibrin clot that forms after the enzyme thrombin cleaves the blood protein fibrinogen. The meth...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.04.010
更新日期:2005-09-01 00:00:00
abstract::It has been reported that chronic inflammation of the vessel wall is a hallmark of atherosclerosis. Interleukin-6 (IL-6) is regarded as an important modulator of inflammatory events occurring during all stages of atherogenesis. Although many factors that induce IL-6 expression have been identified, the effect of homoc...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.03.007
更新日期:2006-07-01 00:00:00