Abstract:
:Induction of HO-1 protein can have both beneficial and detrimental effects for cells, including regulating proliferation and apoptosis of several tumours. We have investigated the regulation of HO-1, p38MAPK and mTOR in the context of proliferation, cell cycle events and apoptosis of oesophageal squamous cell carcinoma cells. Real-time PCR and Western blots were used to determine the expression levels of HO-1, p38MAPK, p-p38MAPK and p-mTOR. MTT assays were used to measure proliferation, FACS for cell cycle events and Annexin V staining for apoptosis. Proliferation of Eca109 cells was inhibited and apoptosis was induced in the presence of p38MAPK inhibitor (SB203580) and mTOR inhibitor (Rapamycin, RAPA). HO-1 expression was downregulated in cells treated with SB203580 and RAPA. HO-1 overexpression inhibited apoptosis and induced G2/M arrest in SB203580 and RAPA-treated cells. HO-1 expression was upregulated in the presence of ethanol, and was accompanied by activation of p38MAPK and mTOR. However, ethanol-treated cells exposed to HO-1 inhibitor showed no effect on p38MAPK and mTOR activation. The data suggest that ethanol-induced upregulation of HO-1 in oesophageal squamous cell carcinoma is accompanied by the activation of the p38MAPK and mTOR pathways.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Hu JL,Xiao L,Li ZY,Wang Q,Chang Y,Jin Ydoi
10.1002/cbin.10075subject
Has Abstractpub_date
2013-06-01 00:00:00pages
584-92issue
6eissn
1065-6995issn
1095-8355journal_volume
37pub_type
杂志文章abstract::Ischemic stroke is a major common cause of death and long-term disability worldwide. Several pathophysiological events including excitotoxicity, oxidative/nitrative stress, inflammation, and apoptosis are involved in ischemic injuries. Recently, the molecular mechanisms involved in cerebral ischemia through a focus on...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.11237
更新日期:2020-02-01 00:00:00
abstract::Conventional kinesin is an ATPase-active motor protein that is involved in the microtubule-dependent transport of cytoplasmic cargoes. The present paper provides a condensed overview on kinesin-1 and its motility generation outside a living cell. Moreover, it demonstrates that the neuron-specific kinesin KIF5A enables...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1016/j.cellbi.2007.12.001
更新日期:2008-05-01 00:00:00
abstract::Long noncoding RNAs (lncRNAs) play important roles in diverse cellular processes and carcinogenesis. Homeobox A cluster antisense RNA 2 (HOXA-AS2) is a 1,048-basepairs lncRNA located between human HOXA3 and HOXA4 genes, whose overactivation was previously found to promote the proliferation and invasion of solid tumors...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11370
更新日期:2020-08-01 00:00:00
abstract::We analyzed whether the embryonic (E12.5-E14.5) mouse retina possesses genuine neural stem cells and how they respond to defined growth factors and extracellular matrix molecules. Whereas most combinations produced no or limited cell survival and proliferation in culture, FGF-2 plus heparin and laminin stimulated prol...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.11.031
更新日期:2007-07-01 00:00:00
abstract::Metallothionein (MT), a cysteine-rich, metal-binding protein, is involved in homeostatic regulation of essential metals and protection of cells against oxidative injury. It has been shown that oxidative stress is associated with pathogenesis of osteoporosis and is capable of inhibiting osteoblastic differentiation of ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.09.004
更新日期:2004-01-01 00:00:00
abstract::Key to mitochondrial activities is the maintenance of mitochondrial morphology, specifically cristae structures formed by the invagination of the inner membrane that are enriched in proteins of the electron transport chain. In Saccharomyces cerevisiae , these cristae folds are a result of the membrane fusion activitie...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11219
更新日期:2019-08-23 00:00:00
abstract::Non-specific L-type calcium channel blockers, such as verapamil (> or =50 microM), induce metaphase-II (M-II) arrest and apoptosis in aged rat eggs cultured in Ca(2+)-deficient medium. However, the effects of extracellular Ca(2+) on verapamil-induced M-II arrest and apoptosis have not yet been reported. We have demons...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.01.001
更新日期:2009-03-01 00:00:00
abstract::Bisphosphonates (BPs), especially zoledronic acid (ZOL), are clinically used to treat osteolytic bone lesions. However, serious side-effects may be also induced during the therapeutic process. To improve the BPs drugs, here, we investigated the effects of a series of ZOL derivatives with increasing number of methylene...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11546
更新日期:2021-01-06 00:00:00
abstract::Rosbin (Thieno [2, 3-c] piperidine-3-carboxamide-2-[(3-methoxy-naphtha-lene-2-carbonyl)-amino]-6-(benzyl)-, hydrochloride), a synthetic piperidinothieno derivative compound obtained from chemical genetics screenings, significantly suppresses the viability of non-small cell lung cancer A549 cells with an IC50 of 2.05 ±...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10714
更新日期:2017-02-01 00:00:00
abstract::The influence of oxygen tension on the side population (SP) fraction sorted from ATDC5 chondroprogenitor cells was investigated. ATDC5 cells cultured in normoxia (20%) or hypoxia (1% oxygen) were compared. The SP fraction was significantly higher in the cells cultured in hypoxia. The gene expression of 3 ABC transport...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.06.009
更新日期:2009-12-01 00:00:00
abstract::The microbicidal capacity of the macrophage is frequently evaded by mycobacteria, leading to tuberculosis (TB). We investigated a number of parameters affecting the rate of growth of mycobacteria in human monocyte-derived macrophages (MDM). The results show a great deal of variation in the growth of both Mycobacterium...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0679
更新日期:2001-01-01 00:00:00
abstract::The Kv2.1 potassium channel is a principal component of the delayed rectifier I(K) current in the pyramidal neurons of cortex and hippocampus. We used whole-cell patch-clamp recording techniques to systemically compare the electrophysiological properties between the native neuronal I(K) current of cultured rat hippoca...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.08.019
更新日期:2008-12-01 00:00:00
abstract::In the past decades increasing lines of evidence have demonstrated that adipose tissue, as an endocrine organ plays a central role in metabolic homeostasis and its related maladies. CCAAT/enhancer-binding protein (C/EBP) family members and the nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ) w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10415
更新日期:2015-04-01 00:00:00
abstract::δ-Catenin and β-catenin belong to different subfamilies of armadillo proteins but share some common binding partners, such as E-cadherin. This is the first study that demonstrated a novel common binding partner for δ-catenin and β-catenin, lymphoid enhancer factor-1 (LEF-1). We found that the N-terminus of δ-catenin (...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10465
更新日期:2015-08-01 00:00:00
abstract::We have studied the effect of enhanced expression of epithelial Na(+)channels (ENaC) on the ATP-induced Cl(-)secretion in the mouse epithelium using short-circuit current (I(SC)) and RT-PCR techniques. The amiloride sensitivity of basal current (I(b)) across the cultured endometrial epithelia was found to vary with th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0754
更新日期:2001-01-01 00:00:00
abstract::Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal ste...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.04.007
更新日期:2004-01-01 00:00:00
abstract::TZDs (thiazolidinediones) are prescribed as anti-Type II diabetes drugs, but little is known regarding whether TZDs regulate the expression of sPLA2 (secretory phospholipase A2) in macrophages. We have investigated the effects of pioglitazone on LPS (lipopolysaccharide)-induced production of TNF-alpha (tumour necrosis...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090005
更新日期:2010-07-01 00:00:00
abstract::Photodynamic therapy (PDT) for cancer is a therapeutic modality in the treatment of tumors in which visible light is used to activate a photosensitizer. Cell membranes have been identified as an important intracellular target for singlet oxygen produced during the photochemical pathway. This study analyzed the cytotox...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.005
更新日期:2008-08-01 00:00:00
abstract::This review summarizes the results of our investigations of actin unfolding-refolding and presents the notion that protein unfolding pathway, the number and the appearance order of intermediate states do not dependent on denaturing agents. To place our concept in the context of current knowledge of protein folding, we...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1016/j.cellbi.2007.01.025
更新日期:2007-04-01 00:00:00
abstract::Calcium loss and muscle atrophy are two of the main metabolic changes experienced by astronauts and crew members during exposure to microgravity in space. Calcium and cytoskeletal events were investigated within sea urchin embryos which were cultured in space under both microgravity and 1 g conditions. Embryos were fi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0371
更新日期:1999-01-01 00:00:00
abstract::Hepatic heat shock protein Hsp70 synthesis and in vitro phosphorylation were studied in the liver cytosol of intact, adrenalectomized and dexamethasone-administered adrenalectomized rats after 41 degrees C whole body hyperthermic stress. Hsp70 was detected by immunoblotting with N27F3-4 monoclonal antibody recognizing...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0247
更新日期:1999-01-01 00:00:00
abstract::TNF-α has long been implicated in the progression of rheumatoid arthritis (RA). However, how the receptors of TNF-α, namely TNFR1 and TNFR2, mediate TNF-α-induced inflammatory responses in fibroblast-like synoviocytes (FLS) in RA has not been elucidated. In the present study, primary FLS cells were isolated from RA pa...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10735
更新日期:2017-04-01 00:00:00
abstract::Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which re...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0964
更新日期:2002-01-01 00:00:00
abstract::microRNAs (miRNAs) have recently been recognized as playing an important role in bone-associated diseases. This study investigated whether the reduced miR-155-5p in steroid-associated osteonecrosis of the femoral head (ONFH) attenuated osteogenic differentiation and cell proliferation by targeting GSK3B. Bone marrow w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11470
更新日期:2021-01-01 00:00:00
abstract::Using flow cytometry and confocal microscopy, the presence of endorphin, serotonin and chorionic gonadotropin (hCG) was demonstrated in rat white blood cells and peritoneal mast cells. After a single neonatal treatment with beta-endorphin (hormonal imprinting), the mast cells of female rats reaching adulthood containe...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00034-9
更新日期:2003-01-01 00:00:00
abstract::Kallikrein 14 (KLK14) is a serine protease linked to several pathologies including prostate cancer and positively correlates with Gleason score. Though KLK14 functioning in cancer is poorly understood, it has been implicated in HGF/Met signaling, given that KLK14 proteolytically inhibits HGF activator-inhibitor 1 (HAI...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10839
更新日期:2017-11-01 00:00:00
abstract::At the early stage of atherosclerosis, neointima is formed due to the migration of vascular smooth muscle cells (VSMCs) from the media to the intima. VSMCs are surrounded by highly adhesive 3D matrices. They take specific strategies to cross various 3D matrices in the media, including heterogeneous collagen and mechan...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10764
更新日期:2017-06-01 00:00:00
abstract::Tripartite motif protein 25 (TRIM25) expression was altered in various human cancers. Herein, we found that the expression of TRIM25 was elevated in hepatocellular carcinoma (HCC) tissues and cell lines. Knockdown of TRIM25 increased the sensitivity of HCC HepG2 cells to epirubicin (EPI), as indicated by reduced cell ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11346
更新日期:2020-07-01 00:00:00
abstract::Eugenol, a natural compound available in honey and various plants extracts including cloves and Magnoliae flos, is exploited for various medicinal applications. Since most of the drugs used in the cancer are apoptotic inducers, the apoptotic effect and anticancer mechanism of eugenol were investigated against colon ca...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100118
更新日期:2011-06-01 00:00:00
abstract::Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temp...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10360
更新日期:2015-02-01 00:00:00