Abstract:
:TZDs (thiazolidinediones) are prescribed as anti-Type II diabetes drugs, but little is known regarding whether TZDs regulate the expression of sPLA2 (secretory phospholipase A2) in macrophages. We have investigated the effects of pioglitazone on LPS (lipopolysaccharide)-induced production of TNF-alpha (tumour necrosis factor alpha), sPLA2-V and -X (groups V and X sPLA2) in RAW 264.7 macrophages. TNF-alpha, sPLA2-V and -X mRNA and protein expression were determined by RT-PCR (reverse transcriptase-PCR) and Western blot analysis, respectively. The activity of NF-kappaB (nuclear factor kappaB) was determined by Western blot and confocal microscopy. LPS induced TNF-alpha, sPLA2-V and sPLA2-X mRNA and protein expression. Pretreatment with 10 mumol/l pioglitazone significantly suppressed LPS-induced TNF-alpha, sPLA2-V and sPLA2-X mRNA and protein expression. LPS induced NF-kappaB expression and translocation in the nucleus, but the inductive effects were inhibited by pioglitazone. Our findings indicate that pioglitazone inhibits production of inflammatory factors induced by LPS in murine macrophage cells by inactivating NF-kappaB. Pioglitazone appears to play an anti-inflammatory role in the atherosclerotic process.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Ao C,Huo Y,Qi L,Xiong Z,Xue L,Qi Ydoi
10.1042/CBI20090005subject
Has Abstractpub_date
2010-07-01 00:00:00pages
723-30issue
7eissn
1065-6995issn
1095-8355pii
CBI20090005journal_volume
34pub_type
杂志文章abstract::Calmodulin-like skin protein (CLSP) is a secreted peptide that is produced by skin keratinocytes and some related epithelial cells. It has previously been shown that CLSP is recruited via the bloodstream into the central nervous system where it likely exerts a neuroprotective effect against toxicity related to Alzheim...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11159
更新日期:2019-07-01 00:00:00
abstract::Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72,900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the under...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10954
更新日期:2018-07-01 00:00:00
abstract::The low density lipoprotein receptor-related protein (alpha(2)MR/LRP) is a cell surface receptor which is present on most cells and tissues. We show that the 85 kDa subunit, containing the transmembrane region and cytoplasmic domain is phosphorylated in vivo. Comparison of the phosphorylation of the low density lipopr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0561
更新日期:2000-01-01 00:00:00
abstract::We have previously reported that the T cell line Jurkat registers the exposure of a sinusoidal extremely low frequency magnetic field at the level of the plasma membrane, resulting in activation of the tyrosine kinase p56(lck), increase in inositol-3-phosphate levels and increase in intracellular calcium concentration...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0837
更新日期:2002-01-01 00:00:00
abstract::The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 5'-nucleotidase were assessed by measuring the levels ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.03.001
更新日期:2006-06-01 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is a serious global health problem. Recently, accumulating microRNA (miRNA) has emerged as crucial players in the development and progression of carcinomas including OSCC. Our study aimed to further investigate the roles of miR-140-5p in OSCC tumorigenesis and related molecular basi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11213
更新日期:2019-08-08 00:00:00
abstract::Bisphosphonates (BPs), especially zoledronic acid (ZOL), are clinically used to treat osteolytic bone lesions. However, serious side-effects may be also induced during the therapeutic process. To improve the BPs drugs, here, we investigated the effects of a series of ZOL derivatives with increasing number of methylene...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11546
更新日期:2021-01-06 00:00:00
abstract::The influence of daunorubicin (DNR) on survival of human normal (S-126) and trisomic, with respect to chromosome 21 (T-164; S-240), skin fibroblasts and some parameters related to it, such as intracellular drug accumulation, distribution and interaction with cell membrane, were studied. The in vitro growth-inhibition ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0583
更新日期:2001-01-01 00:00:00
abstract::Steroid hormones and their receptors play pivotal roles in avian reproduction and development. 17β-estradiol (E2 ) is an essential mediator in ovarian development. In this study, Day 3 (D3) chicks were injected with E2 (0.025, 0.25 or 2.5 mg/kg body weight) for 4 days to assess the effects of estrogen on avian follicl...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10766
更新日期:2017-06-01 00:00:00
abstract::CO25 cells, a mouse myoblast line, contain multiple centrioles and primary cilia. A most unusual feature has been the finding of large numbers of separate structures in single cells-up to a maximum of nine centrioles, six primary cilia, and 12 of both organelles together. Aberrant multipolar spindles were occasionally...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0103
更新日期:1996-12-01 00:00:00
abstract::The diabetic milieu is believed to change the activity, or result in damage of podocytes-a key component of the glomerular filtration barrier and known to secrete matrix for glomerular basement membrane. This in turn contributes to diabetic nephropathy. However, how podocyte dysfunction is triggered in diabetic nephro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10363
更新日期:2015-02-01 00:00:00
abstract::Genistein is one of the naturally occurring isoflavones present in plants such as soybeans and is commonly found in a variety of human foods. A number of studies indicated that this class of compounds exerts anticancerogenic and antimutagenic effects in various in vitro systems and in vivo animal models. We studied th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.04.004
更新日期:2008-08-01 00:00:00
abstract::We have investigated oxidized low-density lipoprotein (ox-LDL) induced senescence in hematopoietic stem cells (HCs). Mouse Sca-1+ HCs were separated and purified using the magnetic activated cell sorting technique. Ox-LDL induced significant senescence in HCs measured by SA-β-Gal staining, and reduced CFU-Mix colony-f...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10121
更新日期:2013-09-01 00:00:00
abstract::The plasticity of human and murine bone marrow mesenchymal stem cells (BMSCs) has been proven by their ability to trans-differentiate to multilineage cells, including germ cells. We have investigated ability of goat BMSCs to trans-differentiate to germ cells with extrinsic (e.g., retinoic acid [RA] and BMP4 signalling...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10348
更新日期:2015-01-01 00:00:00
abstract::Neuroinflammation is closely related with the pathogenesis and progress of neurodegenerative diseases including Alzheimer's disease (AD). Loganin, an iridoid glycoside obtained from traditional Chinese medicine Cornus officinalis, has properties of inhibiting inflammation and improving memory. The present study was ai...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11060
更新日期:2018-12-01 00:00:00
abstract::Cutaneous squamous cell carcinoma (SCC) is a malignant tumour of keratinising epidermal cells. This type of skin cancer is the second leading cause of death after melanoma, and it is the second most common type of non-melanoma skin cancer after basal cell carcinoma. The cellular and molecular events involved in the pr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.06.006
更新日期:2007-11-01 00:00:00
abstract::Glycometabolism, particularly mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, plays a central role in cell life activities. Glycometabolism can be reprogrammed to maintain the stemness or to induce the differentiation of stem cells, thereby regulating tissue repair and regeneration. However, research ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10568
更新日期:2016-03-01 00:00:00
abstract::The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the ga...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1102
更新日期:1994-07-01 00:00:00
abstract::MicroRNA molecules (miRNAs) play important roles in regulating cell behavior. The expression of certain miRNAs has been shown to be regulated by the androgen receptor (AR), which seems to have a critical role in the tumorigenic process of breast cancer. The differential expression of 84 miRNAs was first examined in th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10841
更新日期:2017-12-01 00:00:00
abstract::We have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The reprogrammed iPS cells morphologically resemble human cardiomyocytes which can beat. RT-PCR and immunostaining show that cardiac markers are expressed that are comp...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.08.008
更新日期:2009-11-01 00:00:00
abstract::Toxoplasma gondii is an obligate intracellular pathogen, replicating only within a specialized membrane-bounded cytoplasmic vacuole, the parasitophorous vacuole (PV), which interacts with host cell mitochondria. High mobility group box 1 (HMGB1), a known nuclear transcription factor, also may be involved in pathologic...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.08.031
更新日期:2008-02-01 00:00:00
abstract::Local anesthetic of bupivacaine may inhibit neurite outgrowth and induce apoptosis in mouse dorsal root ganglia (DRG) neurons. In this work, we intended to investigate the functional role of microRNA 26a (miR-26a) in regulating bupivacaine-induced nerve injury in DRG neurons. DRG neurons were extracted from C57BL/6 mi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10461
更新日期:2015-08-01 00:00:00
abstract::Low-temperature stress during microspore development alters cellular organization in rice anthers. The major cellular damage includes unusual starch accumulation in the plastids of the endothecium in postmeiotic anthers, abnormal vacuolation and hypertrophy of the tapetum, premature callose (1,3-beta-glucan) breakdown...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090417
更新日期:2010-03-29 00:00:00
abstract::The aliphatic polyamines, putrescine, spermine and spermidine belong to a category of molecules implicated in DNA replication. Their synthesis is strongly activated during the G1 period and they have been implicated in the regulation of cell proliferation and differentiation. Terminal transferase is a DNA polymerase p...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1016
更新日期:1995-10-01 00:00:00
abstract::Properly metabolized globin synthesis and iron uptake are indispensable for erythroid cell differentiation and maturation. Mitochondrial participation is crucial in the process of haeme synthesis for cytochromes and haemoglobin. We studied the final biosynthesis site of haemoglobin using an ultrastructural approach, w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00157-4
更新日期:2003-01-01 00:00:00
abstract::Genetic analysis has strongly implicated human FHIT (Fragile Histidine Triad) as a tumor suppressor gene, being mutated in a large proportion of early-stage cancers. The functions of the FHIT protein have, however, remained elusive. Here, we investigated aph1+ , the fission yeast homolog of FHIT, for functions related...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11241
更新日期:2020-02-01 00:00:00
abstract::EGFP (enhanced green fluorescent protein) tagged to either the N (amino)-terminus [EGFP/hERG (human ether-a-go-go-related gene)] or C (carboxyl)-terminus (hERG/EGFP) of hERG channel is used to study mutant channel protein trafficking for several years. However, it has been reported that the process can alter hERG chan...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100022
更新日期:2011-03-01 00:00:00
abstract::An acetylcholine (ACh) agonist, carbachol (Cch), causes hepatocytes to proliferate in the presence of hepatic nonparenchymal cells (HNPCs). To identify the HNPCs and ACh receptor subtypes involved in carbachol-induced hepatocyte proliferation (CIHP), we examined two types of vascular cells as candidates for HNPCs medi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.01.018
更新日期:2009-04-01 00:00:00
abstract::ZO-1 is a peripheral protein that plays a central role in the macromolecular assembly of tight junctions by interacting with integral proteins (occludin, claudins, JAMs) of the membrane of adjoining cells, with the actin cytoskeleton, and with nuclear factors. Human ZO-1 is expressed in all epithelia and some speciali...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090067
更新日期:2010-05-07 00:00:00
abstract::We analyzed the connection of changes in nucleus ploidy with changes in nucleolar apparatus of NIH 3T3 cells. The quantity of nucleoli does not depend on the quantity of nucleolar DNA, but instead depends on euploidy: the majority of euploid cells have 1-3 nucleoli. The quantity of DNA in the nucleolus is correlated w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00158-6
更新日期:2003-01-01 00:00:00