当前位置： SCI文献检索 > CELL BIOLOGY INTERNATIONAL期刊下所有文献 > miR-155-5p regulates mesenchymal stem cell osteogenesis and proliferation by targeting GSK3B in steroid-associated osteonecrosis.

miR-155-5p regulates mesenchymal stem cell osteogenesis and proliferation by targeting GSK3B in steroid-associated osteonecrosis.

Abstract：

:microRNAs (miRNAs) have recently been recognized as playing an important role in bone-associated diseases. This study investigated whether the reduced miR-155-5p in steroid-associated osteonecrosis of the femoral head (ONFH) attenuated osteogenic differentiation and cell proliferation by targeting GSK3B. Bone marrow was collected from the proximal femurs of patients with steroid-associated ONFH (n = 10) and patients with new femoral neck fracture (n = 10) and mesenchymal stem cells (MSCs) were isolated. The expression profile, the biological function of miR-155-5p, and the interaction between miR-155-5p and GSK3B were investigated by cell viability measurement, western blot, real-time polymerase chain reaction, luciferase reporter assay, and Alizarin Red S (ARS) staining of MSCs. The MSCs that were obtained from the femoral neck fracture group and from the steroid-associated ONFH group were transfected with or without miR-155-5p. We found that, in ONFH samples, the level of mature miR-155-5p was significantly lower than that of control samples. By inhibiting GSK3B, miR-155-5p promoted the nuclear translocation of β-catenin, increased the expression of osteogenesis-related genes, and facilitated the proliferation and differentiation of MSCs. Restoring the expression of GSK3B in MSCs partially reversed the effect of miR-155-5p. These findings suggest that reduced miR-155-5p in steroid-associated ONFH attenuates osteogenic differentiation and cell proliferation by increased levels of GSK3B and inhibition of Wnt signaling.

journal_name

Cell Biol Int

journal_title

Cell biology international

authors

Wu F,Huang W,Yang Y,Liu F,Chen J,Wang G,Sun Z

doi

10.1002/cbin.11470

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

83-91

issue

1

eissn

1065-6995

issn

1095-8355

journal_volume

45

pub_type

杂志文章

相关文献

CELL BIOLOGY INTERNATIONAL文献大全
  • Trolox-induced cardiac differentiation is mediated by the inhibition of Wnt/β-catenin signaling in human embryonic stem cells.

    abstract：:Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β-catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6-Hydroxy-2,5...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11200

    authors： Choe MS,Yeo HC,Bae CM,Han HJ,Baek KM,Kim JS,Lim KS,Shin IS,Chang W,Yun SP,Lee HJ,Lee MY

    更新日期：2019-07-10 00:00:00

  • Breaking the adhesions of a cell with its substratum.

    abstract：:Investigation of the relative slowness with which trypsin detaches epithelial cells from their adhesions with a substratum suggests that the adherent surface of the cells is normally rather inaccessible to the enzyme. Trypsinisation of cells through a porous filter allowing access of the enzyme to the underside of the...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1993.1070

    authors： Simpson K,McGrath M,Curtis A

    更新日期：1993-03-01 00:00:00

  • A study of the effect of theophylline on the anti-inflammatory potency of dexamethasone using alpha-aminoisobutyric acid uptake in rat skin fibroblasts: a possible mechanism through the glucocorticoid receptor.

    abstract：:The action of theophylline on the uptake of alpha-aminoisobutyric acid (AIB; an indicator of anti-inflammatory potency) stimulated by the glucocorticoid, dexamethasone, in cultured rat fibroblast monolayers was evaluated. Theophylline alone (0.1 m m) did not show significant activity (3314+/-27 cpm) compared with the ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1998.0309

    authors： Xenos K,Kalogeromitros D,Markantonis SL,Katsambas A,Katsarou A

    更新日期：1998-01-01 00:00:00

  • Effects of small interfering RNAs targeting MAPK1 on gene expression profile in HeLa cells as revealed by microarray analysis.

    abstract：:The mitogen activated protein kinases (MAPK) signaling cascade plays an important role in cell life. We proved that small interfering RNAs targeting MAPK1 (siRNA-2) could inhibit HeLa cell growth, but the effects of siRNA-2 on gene expression profile were unclear. Using Affymetrix GeneChip HG-U133A 2.0, we identified ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2008.04.019

    authors： Huang C,Liu LY,Li ZF,Wang P,Ni L,Song LP,Xu DH,Song TS

    更新日期：2008-09-01 00:00:00

  • Rab11 is essential for fertility in Drosophila.

    abstract：:Rab11, a small GTP binding protein involved in vesicular trafficking, has emerged as a key player in regulating various cellular events during Drosophila development and differentiation. In our earlier study a P-insertion line, Rab11mo, was established as a new hypomorphic allele of Rab11 gene, showing degenerated eye...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2008.04.002

    authors： Tiwari AK,Alone DP,Roy JK

    更新日期：2008-09-01 00:00:00

  • Establishment and characterization of endothelial cell lines from the aorta of miniature pig for the study of xenotransplantation.

    abstract：:Pig endothelial cells are the first cells to interact with human immune components after organ xenotransplantation, which is a procedure currently considered to be the best treatment option for end-stage organ failure. It is, therefore, essential to study the mechanisms of molecular interaction between pig endothelial...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2005.03.016

    authors： Kim D,Kim JY,Koh HS,Lee JP,Kim YT,Kang HJ,Hwang WS,Kim YB,Lee JS,Ahn C

    更新日期：2005-08-01 00:00:00

  • Acetylation of alpha-tubilin in different bovine cell types: implications for microtubule dynamics in interphase and mitosis.

    abstract：:Previous work on five cell types isolated from the bovine corpus luteum showed that the mass of acetylated microtubules (acet-MTs) in interphase differed. Endothelial cells, termed type 3, showed few acet-MTs, whereas the interphase cytoskeleton of granulosal-like cells, termed type 5, was rich in acet-MTs. In the pre...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1995.1006

    authors： Wolf KW,Spanel-Borowski K

    更新日期：1995-01-01 00:00:00

  • Acylase 1 expression in rat intestinal crypt--villus axis.

    abstract：:Acylase 1 was investigated at the cellular level in the rat small intestine along the enterocyte-differentiation axis. As confirmed by microscopic analysis, villus tip cells and crypt cells of rat jejunal mucosa were successfully separated using the Weiser method. The proliferating undifferentiated crypt cells showed ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2007.03.006

    authors： Cigna N,Nicoletti C,Durand A,Chaix JC,Giardina T,Perrier J

    更新日期：2007-09-01 00:00:00

  • Nuclear co-repressor (NCoR) is required to maintain insulin sensitivity in C2 C12 myotubes.

    abstract：:Nuclear co-repressor (NCoR) regulates peripheral insulin sensitivity; however, its role in modulating insulin sensitivity in skeletal muscle remains elusive. Present study investigated protein expression and effect of NCoR on insulin sensitivity in murine skeletal muscle cell line C2 C12 . Myotubes as compared to myob...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10711

    authors： Choudhary AK,Dey CS

    更新日期：2017-02-01 00:00:00

  • Evaluation of STAT3 decoy oligodeoxynucleotides' synergistic effects on radiation and/or chemotherapy in metastatic breast cancer cell line.

    abstract：:Resistance to radiotherapy and chemotherapy has been a major problem of conventional cancer therapies, which consequently leads to cancer relapse and cancer-related death. It is known that cancer stem cells (CSCs) play a key role in therapy resistance and CSC-based targeted therapies have been considered as a powerful...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11456

    authors： Johari B,Rahmati M,Nasehi L,Mortazavi Y,Faghfoori MH,Rezaeejam H

    更新日期：2020-12-01 00:00:00

  • Keratins and skin disorders.

    abstract：:The epidermal keratinocytes express two major pairs of keratin polypeptides. One pair (K5/K14) expressed specifically in basal generative compartment and the other (K1/K10) expressed specifically in the differentiating suprabasal compartment. The switch in the expression of the keratins from proliferating to different...

    journal_title：Cell biology international

    pub_type： 杂志文章,评审

    doi：

    authors： Rao KS,Babu KK,Gupta PD

    更新日期：1996-04-01 00:00:00

  • Effect of estrogen on chick primordial follicle development and activation.

    abstract：:Steroid hormones and their receptors play pivotal roles in avian reproduction and development. 17β-estradiol (E2 ) is an essential mediator in ovarian development. In this study, Day 3 (D3) chicks were injected with E2 (0.025, 0.25 or 2.5 mg/kg body weight) for 4 days to assess the effects of estrogen on avian follicl...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10766

    authors： Zhao D,Lv C,Liu G,Mi Y,Zhang C

    更新日期：2017-06-01 00:00:00

  • Loganin prevents BV-2 microglia cells from Aβ1-42 -induced inflammation via regulating TLR4/TRAF6/NF-κB axis.

    abstract：:Neuroinflammation is closely related with the pathogenesis and progress of neurodegenerative diseases including Alzheimer's disease (AD). Loganin, an iridoid glycoside obtained from traditional Chinese medicine Cornus officinalis, has properties of inhibiting inflammation and improving memory. The present study was ai...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11060

    authors： Cui Y,Wang Y,Zhao D,Feng X,Zhang L,Liu C

    更新日期：2018-12-01 00:00:00

  • Low-dose aspirin promotes endothelial progenitor cell migration and adhesion and prevents senescence.

    abstract：:Circulating endothelial progenitor cells (EPCs) play a key role in restoring endothelial function and enhancing angiogenesis. However, the effects of low-dose aspirin on circulating EPCs are not well known. We investigated the effects of low-dose aspirin on EPC migration, adhesion, senescence, proliferation, apoptosis...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2008.03.004

    authors： Hu Z,Zhang F,Yang Z,Zhang J,Zhang D,Yang N,Zhang Y,Cao K

    更新日期：2008-07-01 00:00:00

  • Genistein protects ovarian granulosa cells from oxidative stress via cAMP-PKA signaling.

    abstract：:Genistein is an isoflavone that has estrogen (E2 )-like activity and is beneficial for follicular development, but little is known regarding its function in oxidative stress (OS)-mediated granulosa cell (GC) injury. Here, we found that after exposure to H2 O2 , Genistein weakened the elevated levels of intracellular r...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11244

    authors： Luo M,Yang ZQ,Huang JC,Wang YS,Guo B,Yue ZP

    更新日期：2020-02-01 00:00:00

  • Association of nucleus and centrosome: magnet or velcro?

    abstract：:A structural link between cell's nucleus and centrosome was proposed years ago. Such a link was suggested to maintain nucleus-centrosome axis, determine polarity of interphase cells and ensure spindle assembly in mitotic cells. The idea of structural link is supported by the facts that centrosomes are usually located ...

    journal_title：Cell biology international

    pub_type： 杂志文章,评审

    doi：10.1002/cbin.10016

    authors： Burakov AV,Nadezhdina ES

    更新日期：2013-02-01 00:00:00

  • CFTR inhibits the invasion and growth of esophageal cancer cells by inhibiting the expression of NF-κB.

    abstract：:Our study aimed to explore the function of cystic fibrosis transmembrane conductance regulator (CFTR) in esophageal cancer. Twenty patients with esophageal squamous cell carcinoma (ESCC) and 20 patients with esophageal adenocarcinoma (EA) were enrolled in this study. The levels of CFTR and NF-κB in tumor tissues and a...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11069

    authors： Li W,Wang C,Peng X,Zhang H,Huang H,Liu H

    更新日期：2018-12-01 00:00:00

  • Study of the protective effect on damaged intestinal epithelial cells of rat multilineage-differentiating stress-enduring (Muse) cells.

    abstract：:In this study, we determined whether multilineage-differentiating stress-enduring (Muse) cells exist in rat bone marrow and elucidated their effects on protection against the injury of intestinal epithelial cells associated with inflammation. Rat Muse cells were separated from bone marrow mesenchymal stem cells (BMMSC...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11255

    authors： Sun D,Yang L,Cao H,Shen ZY,Song HL

    更新日期：2020-02-01 00:00:00

  • Mycobacterial growth in human macrophages: variation according to donor, inoculum and bacterial strain.

    abstract：:The microbicidal capacity of the macrophage is frequently evaded by mycobacteria, leading to tuberculosis (TB). We investigated a number of parameters affecting the rate of growth of mycobacteria in human monocyte-derived macrophages (MDM). The results show a great deal of variation in the growth of both Mycobacterium...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.2000.0679

    authors： Hoal-van Helden EG,Hon D,Lewis LA,Beyers N,van Helden PD

    更新日期：2001-01-01 00:00:00

  • Biphasic regulation of H2O2 on angiogenesis implicated NADPH oxidase.

    abstract：:ROS (reactive oxygen species) take an important signalling role in angiogenesis. Although there are several ways to produce ROS in cells, multicomponent non-phagocytic NADPH oxidase is an important source of ROS that contribute to angiogenesis. In the present work, we examined the effects of H2O2 on angiogenesis inclu...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1042/CBI20090092

    authors： Mu P,Liu Q,Zheng R

    更新日期：2010-10-01 00:00:00

  • Expression and immunohistochemical localization of galectin-3 in various mouse tissues.

    abstract：:The expression and immunohistochemical localization of galectin-3, a beta-galactoside-binding protein, was studied in several mouse tissues. Galectin-3 expression was low in the cerebrum, heart, and pancreas, and moderate in the liver, ileum, kidney, and adrenal gland. High expression of galectin-3 was found in the lu...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2006.11.036

    authors： Kim H,Lee J,Hyun JW,Park JW,Joo HG,Shin T

    更新日期：2007-07-01 00:00:00

  • Human insulin receptor juxtamembrane domain independent insulin signaling.

    abstract：:The exon 16-encoded juxtamembrane (JM) domain of human insulin receptor (hIR) harbors the NPEY motif which couples the insulin-activated hIR kinase to downstream signal transduction molecules. We sought to determine if signal transduction requires the entire exon 16-encoded 22-amino acid JM domain. Transfected CHO cel...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2007.01.033

    authors： Sattar AA,Berhanu C,Gebreselassie S,Berhanu P

    更新日期：2007-08-01 00:00:00

  • The fission yeast FHIT homolog affects checkpoint control of proliferation and is regulated by mitochondrial electron transport.

    abstract：:Genetic analysis has strongly implicated human FHIT (Fragile Histidine Triad) as a tumor suppressor gene, being mutated in a large proportion of early-stage cancers. The functions of the FHIT protein have, however, remained elusive. Here, we investigated aph1+ , the fission yeast homolog of FHIT, for functions related...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11241

    authors： Sjölander JJ,Sunnerhagen P

    更新日期：2020-02-01 00:00:00

  • Murine osteoclastogenesis suppression using conditioned media produced by melanoma or activated and non-activated Jurkat-E6 cells.

    abstract：:Conditioned medium (CM) (cell secretome) is a cocktail of growth factors, cytokines, and other soluble mediators secreted by cells into a culture medium. These growth factors are fundamental in many cellular processes such as cell growth, differentiation, and others and the composition of these factors is individual f...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.11317

    authors： Mambelli-Lisboa NC,Frare EO,da Costa-Neves A,Prieto da Silva ÁRB,Kerkis I

    更新日期：2020-05-01 00:00:00

  • Mesenchymal stem cell based therapy for osteo-diseases.

    abstract：:Stem cell therapy in recent years has gained much attention as the modern therapeutic approach to treat diseases. Mesenchymal stem cells (MSCs) are seen as the most reliable cells applied in therapy over other stem cells because of their versatility. Bone and cartilage diseases (osteo-diseases) are the major target of...

    journal_title：Cell biology international

    pub_type： 杂志文章,评审

    doi：10.1002/cbin.10293

    authors： Ghasroldasht MM,Irfan-Maqsood M,Matin MM,Bidkhori HR,Naderi-Meshkin H,Moradi A,Bahrami AR

    更新日期：2014-10-01 00:00:00

  • OP9-DL1 cell co-culture enhances anti-tumour immunity of mouse bone marrow-derived dendritic cells.

    abstract：:DCs (dendritic cells) are the strongest professional APCs (antigen-presenting cells) to initiate immune responses against pathogens, but they are usually incompetent in initiating efficient immune responses in the progress of solid tumours. We have shown that Notch signalling plays a pivotal role in DC-dependent anti-...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1042/CBI20100462

    authors： Feng F,Yang J,Tong L,Yuan S,Tian Y,Hong L,Wang W,Zhang H

    更新日期：2012-03-01 00:00:00

  • 3D arrays for high throughput assay of cell migration and electrotaxis.

    abstract：:Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1002/cbin.10116

    authors： Zhao S,Gao R,Devreotes PN,Mogilner A,Zhao M

    更新日期：2013-09-01 00:00:00

  • Role of bone marrow-derived mesenchymal stem cells in the prevention of hyperoxia-induced lung injury in newborn mice.

    abstract：:BPD (bronchopulmonary dysplasia) is predominantly characterized by persistent abnormalities in lung structure and arrested lung development, but therapy can be palliative. While promising, the use of BMSC (bone marrow-derived mesenchymal stem cell) in the treatment of lung diseases remains controversial. We have asses...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1042/CBI20110447

    authors： Zhang X,Wang H,Shi Y,Peng W,Zhang S,Zhang W,Xu J,Mei Y,Feng Z

    更新日期：2012-06-01 00:00:00

  • Expression analysis of Fgf8a &Fgf8b in early stage of P19 cells during neural differentiation.

    abstract：:Fgf8 is a member of the fibroblast growth factor (FGF) family that plays an important role in early neural development. Cellular aggregation and retinoic acid (RA) are needed for mouse embryonic carcinoma (EC) P19 cell neural differentiation. We have examined the Fgf8 gene in P19 cells during neural differentiation an...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1016/j.cellbi.2009.06.015

    authors： Alam AH,Suzuki H,Tsukahara T

    更新日期：2009-09-01 00:00:00

  • Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells.

    abstract：:Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 ...

    journal_title：Cell biology international

    pub_type： 杂志文章

    doi：10.1006/cbir.1994.1029

    authors： Kaeffer B,Uriel IG,Bottreau E

    更新日期：1994-11-01 00:00:00