Abstract:
:Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal stem cells and culturing them has been challenging. In this study, putative epidermal stem cells of mouse were isolated by combining Hoechst 33342 and propidium iodide staining with fluorescence-activated cell sorting. Molecular markers expression pattern analysis showed that cytokeratin 14, integrin beta1 and p63 are expressed in the sorted putative stem cells, but not active beta-catenin, nestin and involucrin. Our results provide further supporting data that mouse putative epidermal stem cells could be successfully isolated by combining Hoechst dye staining with fluorescence-activated cell sorting and cultured in vitro. The cultured mouse putative epidermal stem cells could be used as a potent tool for studying stem cell biology and testing stem cell therapy.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Zhou JX,Jia LW,Yang YJ,Peng S,Cao YJ,Duan EKdoi
10.1016/j.cellbi.2004.04.007keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
523-9issue
7eissn
1065-6995issn
1095-8355pii
S1065699504000915journal_volume
28pub_type
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