Retinoic acid induces myoblasts transdifferentiation into premeiotic Stra8-positive cells.

Abstract:

:Spermatogonia and sperm-like cells can be derived in vitro via the addition of RA (retinoic acid) to pluripotent ES and EG cells. At present, however, these cells have not been derived from unipotent cells. Here, we have generated premeiotic Stra8-positive cells from C2C12 myoblasts following treatment with 10 μM all-trans-RA for 8 days. The differentiated C2C12 cells exhibited spherical morphology similar to spermatogonia, and they expressed gene markers of premeiosis, meiosis and postmeiosis. In addition, some of the transdifferentiated Stra8-positive cells had a tail-like phenotype. Flow cytometry results indicated that up to 20% of RA-induced C2C12 cells were Stra8-positive. Mvh (mouse vasa homologue) protein, a germ cell-specific ATP-dependent RNA helicase and Prm1 (protamine 1) were detected in transdifferentiated cells. The DNA content in induced C2C12 cells showed that Stra8-positive cells were diploid, suggesting that the myoblast transdifferentiation was in the premeiotic stage of spermatogenesis. The derivation of Stra8-positive cells from C2C12 myoblasts has important implications for studying unipotent cell differentiation. Furthermore, C2C12 myoblasts may provide a useful in vitro cell model to study signal transduction and transdifferentiation during RA treatments.

journal_name

Cell Biol Int

authors

Jia W,Cheng D,Chen S,Lei L,Wang H

doi

10.1042/CBI20100610

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

365-72

issue

4

eissn

1065-6995

issn

1095-8355

pii

CBI20100610

journal_volume

35

pub_type

杂志文章
  • The possible double-edged sword effects of vitamin D on COVID-19: A hypothesis.

    abstract::Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) gains access to host cells by attaching to angiotensin-converting enzyme 2 (ACE2). Vitamin D (VitD) can upregulate ACE2 and has an antagonistic effect on Renin, which exerts a vasodilatation and anti-inflammatory effect against coronavirus disease (COVID-19)...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.11469

    authors: Shojaeefar E,Malih N,Rezaei N

    更新日期:2021-01-01 00:00:00

  • Up-regulation of ectonucleotidase activity after cortical stab injury in rats.

    abstract::The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 5'-nucleotidase were assessed by measuring the levels ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.03.001

    authors: Nedeljkovic N,Bjelobaba I,Subasic S,Lavrnja I,Pekovic S,Stojkov D,Vjestica A,Rakic L,Stojiljkovic M

    更新日期:2006-06-01 00:00:00

  • Quantity and proliferation rate of mesenchymal stem cells in human cord blood during gestation.

    abstract::UCB (umbilical cord blood) as a resource of MSCs (mesenchymal stem cells) is widely accepted, but the quantity and characteristics of UCB-MSCs from different gestational ages have not been well studied. We have quantified the number of MSCs in UCB at different gestational ages using a multi-colour flowcytometer and co...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110173

    authors: Ma LL,Meng FB,Shi P,Li G,Pang XN

    更新日期:2012-04-01 00:00:00

  • Retention and transmission of active transcription memory from progenitor to progeny cells via ligand-modulated transcription factors: elucidation of a concept by BIOPIT model.

    abstract::Observations made in live cells have clearly demonstrated that agonist-activated steroid/nuclear receptors reorganize in the nucleoplasm into hundreds of discrete speckled structures commonly referred to as nuclear foci. Subsequent studies have shown that nuclear foci are formed only with agonist- and not with pure an...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090329

    authors: Kumar S,Saradhi M,Chaturvedi NK,Tyagi RK

    更新日期:2012-02-01 00:00:00

  • gp130-specific antisense oligonucleotides inhibit IL-6 signal inducing junB mRNA transcription in the human hepatoma cell line, HepG2.

    abstract::The biosynthesis of interleukin-6 receptor (IL-6R) and gp130 in vitro was blocked using specific antisense oligonucleotides (ASO) in HepG2 liver cells and the efficacy of various ASOs was tested on the generation of IL-6-induced junB mRNA. We used three ASOs specific for the IL-6 receptor, three specific for gp130 and...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0765

    authors: Varga VL,Fülöp AK,Holub MC,Tóth S,Szalai C,Falus A

    更新日期:2001-01-01 00:00:00

  • The significance of alpha 5 beta 1 integrin dependent and independent actin cytoskelton organization in cell transformation and survival.

    abstract::Cell-matrix and cell-cell interactions are important physiological determinants of cell growth, survival and transformation. Cell adhesion to the extra cellular matrix (ECM) via integrins also crucially influences the organization of the cytoskeleton. It triggers a cascade of intracellular biochemical events, which re...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2002.0964

    authors: Rajeswari J,Pande G

    更新日期:2002-01-01 00:00:00

  • Hepcidin and its potential clinical utility.

    abstract::A number of pathophysiological conditions are related to iron metabolism disturbances. Some of them are well known, others are newly discovered or special. Hepcidin is a newly identified iron metabolism regulating hormone, which could be a promising biomarker for many disorders. In this review, we provide background i...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10505

    authors: Miseta A,Nagy J,Nagy T,Poór VS,Fekete Z,Sipos K

    更新日期:2015-11-01 00:00:00

  • Traditional Chinese medicine Bak Foong Pills alters uterine quiescence - possible role in alleviation of dysmenorrhoeal symptoms.

    abstract::Since contractility of the uterus appears to be the major source of pain during dysmenorrhoea, alleviation of the contractions is believed to be a possible treatment strategy. Bak Foong Pills, a traditional Chinese formulation for use in gynaecological disorders, has long been thought as effective in the treatment of ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2009.03.003

    authors: Rowlands DK,Cui YG,Wong HY,Gou YL,Chan HC

    更新日期:2009-12-01 00:00:00

  • Vacuolation in T-tubules as a model for tubular-vesicular transformations in biomembrane systems.

    abstract::This review outlines the basic properties of T-tubules in skeletal muscle cells, and the factors that govern reversible vacuolation in T-tubules under experimental conditions. Comparable membranous transformations, involving the plasma membrane or occurring intracellularly, in non-muscle cells are then considered. Fin...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1006/cbir.2002.0945

    authors: Krolenko SA,Lucy JA

    更新日期:2002-01-01 00:00:00

  • Knockdown of ghrelin-O-acyltransferase attenuates colitis through the modulation of inflammatory factors and tight junction proteins in the intestinal epithelium.

    abstract::Ghrelin-O-acyltransferase (GOAT) is a membrane-bound enzyme that attaches eight-carbon octanoate to a serine residue in ghrelin and thereby acylates inactive ghrelin to produce active ghrelin. In this study, we investigated the function of GOAT in the intestinal mucosal barrier. The intestinal mucosal barrier prevents...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11362

    authors: Tian P,Lu X,Jin N,Shi J

    更新日期:2020-08-01 00:00:00

  • HGF expression induced by HIF-1α promote the proliferation and tube formation of endothelial progenitor cells.

    abstract::Hypoxia-inducible factor-1α (HIF-1α) and hepatocyte growth factor (HGF) play important roles in postnatal neovascularization. However, the interaction of these two pathways is not fully understood. The present study utilized CoCl(2) treated-endothelial progenitor cells (EPCs) (EPCs exposure to CoCl(2) are under mimic ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10397

    authors: Yu F,Lin Y,Zhan T,Chen L,Guo S

    更新日期:2015-03-01 00:00:00

  • Examination of actin polymerization and viscosity induced by cations and ionic strength when cross-linked by alpha-actinin.

    abstract::Increasing potassium chloride concentration from 0 to 100 mM and magnesium chloride from 0 to 2 mM show a parallel rate increase in polymerizing actin, whereas increasing calcium chloride concentration from 0 to 0.2 mM decreases the rate of polymerizing actin. The presence of alpha-actinin has little influence on the ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2002.0898

    authors: Goldmann WH

    更新日期:2002-01-01 00:00:00

  • The effect of inflammatory factors and their inhibitors on the hematopoietic stem cells fate.

    abstract::Inflammatory cytokines exert different effects on hematopoietic stem cells (HSCs), lead to the development of various cell lineages in bone marrow (BM) and are thus a differentiation axis for HSCs. The content used in this article has been obtained by searching PubMed database and Google Scholar search engine of Engli...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.11545

    authors: Najafi S,Ghanavat M,Shahrabi S,Gatavizadeh Z,Saki N

    更新日期:2021-01-02 00:00:00

  • Behaviour of dissociated hypoblast cells on the basal lamina and on extracellular fibrils in the gastrulating chicken embryo.

    abstract::The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the ga...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1994.1102

    authors: Andries L,Harrisson F,Vakaet L

    更新日期:1994-07-01 00:00:00

  • Effect of magnesium on calcium-induced depolarisation of mitochondrial transmembrane potential.

    abstract::An effect of magnesium on calcium-induced depolarisation of mitochondrial transmembrane potential (DeltaPsi(m)) was investigated. Depending on the presence of Mg(2+), addition of Ca(2+) to suspension of isolated rat heart mitochondria induced either reversible depolarisation or irreversible collapse of succinate-drive...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.08.024

    authors: Racay P

    更新日期:2008-01-01 00:00:00

  • Establishment of ultra long-lived cell lines by transfection of TERT into normal human fibroblast TIG-1 and their characterization.

    abstract::To establish useful human normal cell lines, TERT (telomerase reverse transcriptase) cDNA was transfected into normal female lung fibroblast, TIG-1. After long-term-sub-cultivation of 74 individual clones selected for resistance to G418, we obtained 55 cultures with normal range of life span [75 PDL (population doubli...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110460

    authors: Kamada M,Kumazaki T,Matsuo T,Mitsui Y,Takahashi T

    更新日期:2012-06-01 00:00:00

  • Effect of downregulation of ZEB1 on vimentin expression, tumour migration and tumourigenicity of melanoma B16F10 cells and CSCs.

    abstract::Zinc-finger E-box binding homeobox 1 (ZEB1) is a master regulator of epithelial-mesenchymal transition (EMT) and has been implicated in primary epithelial cancer biological processes, such as invasion and metastasis. However, the role of ZEB1 in progression of melanoma and cancer stem cells (CSCs) remains obscure. In ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10223

    authors: Dou J,He X,Liu Y,Wang Y,Zhao F,Wang X,Chen D,Shi F,Wang J

    更新日期:2014-04-01 00:00:00

  • Smart hydrogels with high tunability of stiffness as a biomimetic cell carrier.

    abstract::Human tissues are sophisticated ensembles of various distinct cell types encapsulated in the biomechanical cues of the extracellular matrix. It has been known matrix stiffness plays a pivot role in cellular events and tissue-scale biological processes. Thus, materials that can mimic mechanical environments of tissues ...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.11091

    authors: Zhao H,Xu K,Zhu P,Wang C,Chi Q

    更新日期:2019-02-01 00:00:00

  • Effect of metallothionein on cell viability and its interactions with cadmium and zinc in HEK293 cells.

    abstract::Metallothioneins (MTs) are thought to participate in a wide variety of physiological roles, but the mechanisms involved are still unclear. The study was designed to examine the possible factors related to these mechanisms. Methods, including transfection, MTT assay and flow cytometry, were used to investigate the effe...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2005.05.008

    authors: Li J,Liu Y,Ru B

    更新日期:2005-10-01 00:00:00

  • Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells.

    abstract::Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1994.1029

    authors: Kaeffer B,Uriel IG,Bottreau E

    更新日期:1994-11-01 00:00:00

  • Morphometry of nuclear and nucleolar structures in a CaCo-2 cell line.

    abstract::The number of the nucleoli in a CaCo-2 cell nucleus does not generally depend on the quantity of DNA in the nucleus, but nucleolar DNA content is directly proportional to total nuclear DNA. However, in multinucleolar cells (three or more nucleoli), the nucleolar DNA content increases after 96 h incubation in culture w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.01.004

    authors: Karalyan ZA,Djaghatspanyan NG,Gasparyan MH,Hakobyan LA,Abroyan LO,Magakyan YH,Ter-Pogossyan ZR,Kamalyan LA,Karalova EM

    更新日期:2004-01-01 00:00:00

  • Molecular and ultrastructural characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells.

    abstract::In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.07.020

    authors: Jazayeri M,Allameh A,Soleimani M,Jazayeri SH,Piryaei A,Kazemnejad S

    更新日期:2008-10-01 00:00:00

  • Effect of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: immunocytochemistry MIB1 score and DNA ploidy and apoptosis evaluated by flow cytometry.

    abstract::Mitomycin C (MMC) treatment has been used to arrest cell proliferation but not much is known about the effect of MMC on human foreskin fibroblasts (HFF) used as feeders for human embryonic stem cells (hESC). We tested the ability of MMC to stop the proliferation of HFF and to induce apoptosis. MMC inhibited the prolif...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.11.006

    authors: Nieto A,Cabrera CM,Catalina P,Cobo F,Barnie A,Cortés JL,Barroso del Jesus A,Montes R,Concha A

    更新日期:2007-03-01 00:00:00

  • Effect of TGF-alpha on growth of normal human breast epithelial cells in serum-free primary culture using 3-dimensional collagen gels.

    abstract::The mitogenic effect of TGF-alpha, acidic-FGF, basic-FGF and lithium on normal human breast epithelial cells was studied in a collagen gel culture system using a serum-free 1:1 mixture of Ham's F12 and DME medium containing insulin, cholera toxin and bovine serum albumin. TGF-alpha elicited a strong mitogenic response...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1993.1077

    authors: Sakthivel R,Hamdan M,Yang J,Guzman RC,Nandi S

    更新日期:1993-04-01 00:00:00

  • Fndc5 overexpression facilitated neural differentiation of mouse embryonic stem cells.

    abstract::Fndc5 has been recently recognized as a myokine which could be cleaved and secreted into blood stream. It is termed as irisin with an important role in thermogenesis and energy homeostasis. Increased expression of Fndc5 has been reported upon retinoic acid treatment during neural differentiation and its knockdown decr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10427

    authors: Forouzanfar M,Rabiee F,Ghaedi K,Beheshti S,Tanhaei S,Shoaraye Nejati A,Jodeiri Farshbaf M,Baharvand H,Nasr-Esfahani MH

    更新日期:2015-05-01 00:00:00

  • Apoptosis effects of Xrel3 c-Rel/Nuclear Factor-kappa B homolog in human cervical cancer cells.

    abstract::Cervical cancer is considered a common yet preventable cause of death in women. In this report, we studied the role of the NF-kappaB gene family in HeLa human cervical cancer cells, using the Xrel3 c-Rel homologue of Xenopus laevis. The expression of Xrel3/c-Rel slowed cell growth 6-fold, consistent with an upregulate...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.12.014

    authors: Shehata M,Shehata M,Shehata F,Pater A

    更新日期:2005-06-01 00:00:00

  • Trolox-induced cardiac differentiation is mediated by the inhibition of Wnt/β-catenin signaling in human embryonic stem cells.

    abstract::Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β-catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6-Hydroxy-2,5...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11200

    authors: Choe MS,Yeo HC,Bae CM,Han HJ,Baek KM,Kim JS,Lim KS,Shin IS,Chang W,Yun SP,Lee HJ,Lee MY

    更新日期:2019-07-10 00:00:00

  • Enrichment and characterization of mouse putative epidermal stem cells.

    abstract::Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal ste...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.04.007

    authors: Zhou JX,Jia LW,Yang YJ,Peng S,Cao YJ,Duan EK

    更新日期:2004-01-01 00:00:00

  • Zebularine significantly sensitises MEC1 cells to external irradiation and radiopharmaceutical therapy when administered sequentially in vitro.

    abstract::Zebularine is a cytidine analogue incorporated into DNA during replication, inhibiting DNA methyltransferase 1 (DNMT1), resulting in demethylation and changes in gene expression. Such modification may improve radiosensitivity in resistant lymphoma cells. The hypothesis of this study was that zebularine and radiation w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10215

    authors: Bryan JN,Kumar SR,Jia F,Balkin ER,Lewis MR

    更新日期:2014-02-01 00:00:00

  • Cytostatic effect of inostamycin, an inhibitor of cytidine 5'-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG): inositol transferase, on oral squamous cell carcinoma cell lines.

    abstract::Inostamycin, which was recently isolated from Streptomyces sp. MH816-AF15 as an inhibitor of cytidine 5'-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG): inositol transferase, caused a G1-phase accumulation in the cell cycle of small cell lung carcinomas. To investigate whether the cytostatic effect of inostamycin is rest...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0706

    authors: Baba Y,Tsukuda M,Mochimatsu I,Furukawa S,Kagata H,Nagashima Y,Koshika S,Imoto M,Kato Y

    更新日期:2001-01-01 00:00:00