Abstract:
:The function of the gene gilgamesh (89B9-12) encoding a casein kinase in Drosophila spermatogenesis was studied. The chimeric Gilgamesh-GFP protein in spermatocytes is cortically located. In the polar and apolar spermatocytes, it concentrates at the terminal ends of the fusome, the organelle that passes through the system of ring canals of the spermatocyte cyst. At the stage of spermatid elongation, the protein associates with the nucleus. A spot of the highest Gilgamesh-GFP concentration in the nucleus co-localizes with gamma-tubulin in the basal body. At later stages, Gilgamesh is localized to the individualization complex (IC), leaving the nuclei somewhat before the IC investment cones, as detected by actin binding. The sterile mutation due to the gilgamesh gene leads to the phenotype of scattered nuclei and altered structure of actin cones in the individualizing spermatid cyst. Ultrastructural evidence confirmed defective spermatid individualization due to the mutation. The phylogenetic origin of the protein, and the connection between vesicular trafficking and spermatid individualization, are discussed.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Nerusheva OO,Dorogova NV,Gubanova NV,Yudina OS,Omelyanchuk LVdoi
10.1016/j.cellbi.2009.02.009subject
Has Abstractpub_date
2009-05-01 00:00:00pages
586-93issue
5eissn
1065-6995issn
1095-8355pii
S1065-6995(09)00056-0journal_volume
33pub_type
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