Abstract:
:The formation of vertebrate skeletal muscles widely thought to be under the control of hierarchy of regulatory genes. MYF5 is one of the myogenic determination gene expressed in the developing mouse dermomyotome which control skeletal muscle differentiation. In the current work, we had obtained the cDNA sequence including the full coding region of the bos taurus myogenic factor MYF5 by reverse transcription polymerase chain reaction. Furthermore, we examined whether fibroblast cell derived from mouse and bos taurus can be transduced using plasmid vectors carrying bos taurus MYF5. Bos taurus MYF5 activates MYF5 and MYOD1 expression after 1 day culture. The concerted upregulation of the myogenic regulatory factors enhanced myosin (skeletal fast) expression. These observation show that MYF5 is essential for myogenic differentiation and provides candidates for regulation bos taurus skeletal muscle development.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Nie YW,Ding XB,Ge XG,Fan HL,Liu ZW,Guo Hdoi
10.1002/cbin.10124subject
Has Abstractpub_date
2013-09-01 00:00:00pages
972-6issue
9eissn
1065-6995issn
1095-8355journal_volume
37pub_type
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