Studies on nitric oxide free radicals generated from polymorphonuclear leukocytes (PMN) stimulated by phorbol myristate acetate (PMA).

Abstract:

:The interaction of NO and O2- free radicals generated from PMA (phorbol myristate acetate)-stimulated PMN (polymorphonuclear leukocytes) was studied by a nitroxide spin trap, DMPO (5,5-dimethyl-1-pyrroline-1-oxide). It was found that addition of L-arginine to the system would significantly decrease the trapped O2- by DMPO and addition of NG-monomethyl-arginine (NGMA) would significantly increase the trapped O2- by DMPO. It was proved that the formation of ONOO- by the reaction of NO and O2- was the main reason for the decrease of trapped O2- in the experiment with xanthine/xanthine oxidase and irradiation of riboflavin systems. The yield of NO during this process was calculated. The generation dynamic of NO was studied by a luminol-dependent chemiluminescence technique and it was found that after stimulation of PMN by PMA, there would be an immediate, significant chemiluminescence, which came mainly from the active oxygen free radicals generated by PMN. If L-arginine was added to this system, the chemiluminescence would increase about 100-fold, but NGMA inhibited the increase of the chemiluminescence. Ten minutes after addition of L-arginine, this increase did not change, the chemiluminescence peak decreased gradually, but the half life increased. The ESR and chemiluminescence properties of NO and ONOO- synthesized were also studied in model systems.

journal_name

Cell Biol Int

authors

Zhao BL,Wang JC,Hou JW,Xin WJ

doi

10.1006/cbir.1996.0039

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

343-50

issue

5

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(96)90039-6

journal_volume

20

pub_type

杂志文章
  • Modification of the alkaline comet assay with human mesenchymal stem cells.

    abstract::MSCs (mesenchymal stem cells) are planned foruse in regenerative medicine to offset age-dependent alterations. However, MSCs are affected by replicative senescence associated with decreasing proliferation potential, telomere shortening and DNA damage during in vitro propagation. To monitor in vitro senescence, we have...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110251

    authors: Fuchs R,Stelzer I,Drees CM,Rehnolt C,Schraml E,Sadjak A,Schwinger W

    更新日期:2012-01-01 00:00:00

  • The fission yeast FHIT homolog affects checkpoint control of proliferation and is regulated by mitochondrial electron transport.

    abstract::Genetic analysis has strongly implicated human FHIT (Fragile Histidine Triad) as a tumor suppressor gene, being mutated in a large proportion of early-stage cancers. The functions of the FHIT protein have, however, remained elusive. Here, we investigated aph1+ , the fission yeast homolog of FHIT, for functions related...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11241

    authors: Sjölander JJ,Sunnerhagen P

    更新日期:2020-02-01 00:00:00

  • In vitro culture and induced differentiation of sheep skeletal muscle satellite cells.

    abstract::Skeletal muscle satellite cells are adult muscle-derived stem cells receiving increasing attention. Sheep satellite cells have a greater similarity to human satellite cells with regard to metabolism, life span, proliferation and differentiation, than satellite cells of the rat and mouse. We have used 2-step enzymatic ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110487

    authors: Wu H,Ren Y,Li S,Wang W,Yuan J,Guo X,Liu D,Cang M

    更新日期:2012-06-01 00:00:00

  • Melatonin ameliorates degenerative alterations caused by age in the rat prostate and mitigates high-fat diet damages.

    abstract::Imbalance of sexual steroids milieu and oxidative stress are often observed during aging and correlated to prostate disorders. Likewise, high-fat intake has been related to prostate damage and tumor development. Melatonin (MLT) is an antioxidant whose secretion decreases in elderly and is also suggested to protect the...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11472

    authors: Tamarindo GH,Gobbo MG,Taboga SR,Almeida EA,Góes RM

    更新日期:2021-01-01 00:00:00

  • Mechanism of low-temperature-induced pollen failure in rice.

    abstract::Low-temperature stress during microspore development alters cellular organization in rice anthers. The major cellular damage includes unusual starch accumulation in the plastids of the endothecium in postmeiotic anthers, abnormal vacuolation and hypertrophy of the tapetum, premature callose (1,3-beta-glucan) breakdown...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090417

    authors: Al Mamun E,Cantrill LC,Overall RL,Sutton BG

    更新日期:2010-03-29 00:00:00

  • Effect of TGF-alpha on growth of normal human breast epithelial cells in serum-free primary culture using 3-dimensional collagen gels.

    abstract::The mitogenic effect of TGF-alpha, acidic-FGF, basic-FGF and lithium on normal human breast epithelial cells was studied in a collagen gel culture system using a serum-free 1:1 mixture of Ham's F12 and DME medium containing insulin, cholera toxin and bovine serum albumin. TGF-alpha elicited a strong mitogenic response...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1993.1077

    authors: Sakthivel R,Hamdan M,Yang J,Guzman RC,Nandi S

    更新日期:1993-04-01 00:00:00

  • The effect of oxidative stress induced by t-butyl hydroperoxide on the structural dynamics of membrane proteins of Chinese hamster fibroblasts.

    abstract::A method based on the measurement at room temperature of tryptophan phosphorescence (RTTP) gives the unique possibility to investigate the dynamic structure of membrane proteins without their isolation from cells. This method was used to study the influence of tert- butyl hydroperoxide (t -BHP) on Chinese hamster fibr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0366

    authors: Mazhul V,Shcherbin D,Zavodnik I,Rekawiecka K,Bryszewska M

    更新日期:1999-01-01 00:00:00

  • Enrichment and characterization of mouse putative epidermal stem cells.

    abstract::Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal ste...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.04.007

    authors: Zhou JX,Jia LW,Yang YJ,Peng S,Cao YJ,Duan EK

    更新日期:2004-01-01 00:00:00

  • Ro52/SSA sensitizes cells to death receptor-induced apoptosis by down-regulating c-FLIP(L).

    abstract::Ro52/SSA is an autoantigen that presents in patients with SS (Sjögren's syndrome) and SLE (systemic lupus erythematosus). It increases cell death and redistributes itself to apoptotic blebs, but its pro-apoptotic function has not been completely identified. Overexpression of Ro52/SSA promoted cell apoptosis induced by...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110322

    authors: Zhang J,Fang L,Zhu X,Qiao Y,Yu M,Wang L,Chen Y,Yin W,Hua ZC

    更新日期:2012-05-01 00:00:00

  • Morphometry of nuclear and nucleolar structures in a CaCo-2 cell line.

    abstract::The number of the nucleoli in a CaCo-2 cell nucleus does not generally depend on the quantity of DNA in the nucleus, but nucleolar DNA content is directly proportional to total nuclear DNA. However, in multinucleolar cells (three or more nucleoli), the nucleolar DNA content increases after 96 h incubation in culture w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.01.004

    authors: Karalyan ZA,Djaghatspanyan NG,Gasparyan MH,Hakobyan LA,Abroyan LO,Magakyan YH,Ter-Pogossyan ZR,Kamalyan LA,Karalova EM

    更新日期:2004-01-01 00:00:00

  • The DNA binding affinity of rat liver nucleoproteins to the regulatory elements of the haptoglobin and alpha 2-macroglobulin genes.

    abstract::Transcriptional regulation and binding interactions between soluble nucleoproteins and the hormone response elements (REs) of the rat haptoglobin (Hp) and alpha 2-macroglobulin (alpha 2MG) genes was examined in the livers of rats during the acute-phase reaction. Our results demonstrate that the elevation of the Hp and...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1995.1037

    authors: Petrović M,Bogojević D,Grigorov I,Sevaljević L

    更新日期:1995-12-01 00:00:00

  • Expression of osteoclastogenic factor transcripts in osteoblast-like UMR-106 cells after exposure to FGF-23 or FGF-23 combined with parathyroid hormone.

    abstract::As a bone-derived hormone, fibroblast growth factor-23 (FGF-23) negatively regulates phosphate and calcium metabolism, while retaining growth-promoting action for mesenchymal cell differentiation. Elevated FGF-23 levels, together with hyperparathyroidism, are often observed in chronic kidney disease, which is associat...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10573

    authors: Teerapornpuntakit J,Wongdee K,Krishnamra N,Charoenphandhu N

    更新日期:2016-03-01 00:00:00

  • Smart mechanosensing machineries enable migration of vascular smooth muscle cells in atherosclerosis-relevant 3D matrices.

    abstract::At the early stage of atherosclerosis, neointima is formed due to the migration of vascular smooth muscle cells (VSMCs) from the media to the intima. VSMCs are surrounded by highly adhesive 3D matrices. They take specific strategies to cross various 3D matrices in the media, including heterogeneous collagen and mechan...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10764

    authors: Chi Q,Shan J,Ding X,Yin T,Wang Y,Jia D,Wang G

    更新日期:2017-06-01 00:00:00

  • Estrogen and progesterone receptors in carpal tunnel syndrome.

    abstract::Carpal tunnel syndrome (CTS) is a compression median nerve neuropathy common in women at menopausal age. The aim of this work was to study immunohistochemically the expression of estrogen (ER) and progesterone (PR) receptors in CTS and control specimens. Biopsies of transverse carpal ligament (TCL) and flexor tendon s...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.08.014

    authors: Toesca A,Pagnotta A,Zumbo A,Sadun R

    更新日期:2008-01-01 00:00:00

  • An appreciation of the prescience of Don Gilbert (1930-2011): master of the theory and experimental unravelling of biochemical and cellular oscillatory dynamics.

    abstract::We review Don Gilbert's pioneering seminal contributions that both detailed the mathematical principles and the experimental demonstration of several of the key dynamic characteristics of life. Long before it became evident to the wider biochemical community, Gilbert proposed that cellular growth and replication neces...

    journal_title:Cell biology international

    pub_type: 传,历史文章,杂志文章

    doi:10.1002/cbin.11341

    authors: Gilbert K,Hammond KD,Brodsky VY,Lloyd D

    更新日期:2020-06-01 00:00:00

  • Transforming growth factor-β boosts the functionality of human bone marrow-derived mesenchymal stromal cells.

    abstract::Transforming growth factor β1 (TGFβ1) is a negative regulator of hematopoiesis, and yet, it is frequently found at the active sites of hematopoiesis. Here, we show for the first time that bone marrow-derived mononuclear cells (BM MNCs) secrete TGFβ1 in response to erythropoietin (EPO). We further show that human bone ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11437

    authors: Kale VP

    更新日期:2020-11-01 00:00:00

  • Rab11 is essential for fertility in Drosophila.

    abstract::Rab11, a small GTP binding protein involved in vesicular trafficking, has emerged as a key player in regulating various cellular events during Drosophila development and differentiation. In our earlier study a P-insertion line, Rab11mo, was established as a new hypomorphic allele of Rab11 gene, showing degenerated eye...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.04.002

    authors: Tiwari AK,Alone DP,Roy JK

    更新日期:2008-09-01 00:00:00

  • Breaking the adhesions of a cell with its substratum.

    abstract::Investigation of the relative slowness with which trypsin detaches epithelial cells from their adhesions with a substratum suggests that the adherent surface of the cells is normally rather inaccessible to the enzyme. Trypsinisation of cells through a porous filter allowing access of the enzyme to the underside of the...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1993.1070

    authors: Simpson K,McGrath M,Curtis A

    更新日期:1993-03-01 00:00:00

  • Retention and transmission of active transcription memory from progenitor to progeny cells via ligand-modulated transcription factors: elucidation of a concept by BIOPIT model.

    abstract::Observations made in live cells have clearly demonstrated that agonist-activated steroid/nuclear receptors reorganize in the nucleoplasm into hundreds of discrete speckled structures commonly referred to as nuclear foci. Subsequent studies have shown that nuclear foci are formed only with agonist- and not with pure an...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20090329

    authors: Kumar S,Saradhi M,Chaturvedi NK,Tyagi RK

    更新日期:2012-02-01 00:00:00

  • Propofol induces apoptosis of hepatocellular carcinoma cells by upregulation of microRNA-199a expression.

    abstract::Propofol is one of the extensively commonly used intravenous anaesthetic agents. The effects of Propofol on hepatocellular carcinoma (HCC) growth inhibition and apoptosis have been examined. The techniques used were the MTT assay, flow cytometry, real-time PCR to assess miR-199a expression, as also caspase-8 and caspa...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10034

    authors: Zhang J,Wu GQ,Zhang Y,Feng ZY,Zhu SM

    更新日期:2013-03-01 00:00:00

  • Glycometabolic reprogramming associated with the initiation of human dental pulp stem cell differentiation.

    abstract::Glycometabolism, particularly mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, plays a central role in cell life activities. Glycometabolism can be reprogrammed to maintain the stemness or to induce the differentiation of stem cells, thereby regulating tissue repair and regeneration. However, research ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10568

    authors: Wang L,Cheng L,Wang H,Pan H,Yang H,Shao M,Hu T

    更新日期:2016-03-01 00:00:00

  • Vertebrate primary cilia: a sensory part of centrosomal complex in tissue cells, but a "sleeping beauty" in cultured cells?

    abstract::Primary cilium development along with other components of the centrosome in mammalian cells was analysed ultrastructurally and by immunofluorescent staining with anti-acetylated tubulin antibodies. We categorized two types of primary cilia, nascent cilia that are about 1microm long located inside the cytoplasm, and tr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2003.11.013

    authors: Alieva IB,Vorobjev IA

    更新日期:2004-01-01 00:00:00

  • Effects of extremely low concentrations of hormones on the insulin binding of Tetrahymena.

    abstract::FITC-insulin binding to previously hormone-treated Tetrahymena was studied by flow cytometry and confocal microscopy. Hormones produced by Tetrahymena were chosen for study and the hormone concentrations were administered between 10(-6) and 10(-21)M for 30 min. Endorphin, serotonin and insulin significantly reduced th...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.06.019

    authors: Csaba G,Kovács P,Tóthfalusi L,Pállinger E

    更新日期:2006-12-01 00:00:00

  • Trolox-induced cardiac differentiation is mediated by the inhibition of Wnt/β-catenin signaling in human embryonic stem cells.

    abstract::Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β-catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6-Hydroxy-2,5...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11200

    authors: Choe MS,Yeo HC,Bae CM,Han HJ,Baek KM,Kim JS,Lim KS,Shin IS,Chang W,Yun SP,Lee HJ,Lee MY

    更新日期:2019-07-10 00:00:00

  • In vitro differentiated hepatic oval-like cells enhance hepatic regeneration in CCl4 -induced hepatic injury.

    abstract::Hepatic oval cells are likely to be activated during advanced stage of liver fibrosis to reconstruct damaged hepatic tissue. However, their scarcity, difficulties in isolation, and in vitro expansion hampered their transplantation in fibrotic liver. This study was aimed to investigate the repair potential of in vitro ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10699

    authors: Awan SJ,Baig MT,Yaqub F,Tayyeb A,Ali G

    更新日期:2017-01-01 00:00:00

  • Heat shock protein 27 as a neuroprotective biomarker and a suitable target for stem cell therapy and pharmacotherapy in ischemic stroke.

    abstract::Ischemic stroke is a major common cause of death and long-term disability worldwide. Several pathophysiological events including excitotoxicity, oxidative/nitrative stress, inflammation, and apoptosis are involved in ischemic injuries. Recently, the molecular mechanisms involved in cerebral ischemia through a focus on...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.11237

    authors: Behdarvandy M,Karimian M,Atlasi MA,Azami Tameh A

    更新日期:2020-02-01 00:00:00

  • Oxidized low-density lipoprotein induces hematopoietic stem cell senescence.

    abstract::We have investigated oxidized low-density lipoprotein (ox-LDL) induced senescence in hematopoietic stem cells (HCs). Mouse Sca-1+ HCs were separated and purified using the magnetic activated cell sorting technique. Ox-LDL induced significant senescence in HCs measured by SA-β-Gal staining, and reduced CFU-Mix colony-f...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10121

    authors: Zhang XP,Zhang GH,Wang YY,Liu J,Wei Q,Xu CY,Wang JW,Wang YP

    更新日期:2013-09-01 00:00:00

  • The neurosteroid dehydroepiandrosterone could improve somatic cell reprogramming.

    abstract::Expression of four major reprogramming transgenes, including Oct4, Sox2, Klf4 and c-myc, in somatic cells enables them to have pluripotency. These cells are iPSC (induced pluripotent stem cell) that currently show the greatest potential for differentiation into cells of the three germ lineages. One of the issues facin...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100927

    authors: Shoae-Hassani A,Sharif S,Verdi J

    更新日期:2011-10-01 00:00:00

  • A fine structural study of the hippocampus and dorsal root ganglion in mouse trisomy 16, a model of Down's syndrome.

    abstract::Mouse trisomy 16 (Ts16) appears to provide an animal model of Down's syndrome in that a portion of mouse chromosome 16 is syntenic with part of human chromosome 21. Trisomy 21 in human beings leads to the mental retardation of Down's syndrome and in middle age, to some presenile anatomic and clinical features of Alzhe...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1996.0089

    authors: Lane NJ,Balbo A,Rapoport SI

    更新日期:1996-10-01 00:00:00

  • Bacterial invasion of eukaryotic cells can be mediated by actin-hydrolysing metalloproteases grimelysin and protealysin.

    abstract::Earlier, we have shown that spontaneously isolated non-pathogenic bacteria Serratia grimesii and Serratia proteamaculans invade eukaryotic cells, provided that they synthesize thermolysin-like metalloproteases ECP32/grimelysin or protealysin characterized by high specificity towards actin. To address the question of w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100314

    authors: Bozhokina ES,Tsaplina OA,Efremova TN,Kever LV,Demidyuk IV,Kostrov SV,Adam T,Komissarchik YY,Khaitlina SY

    更新日期:2011-02-01 00:00:00