Abstract:
:Inostamycin, which was recently isolated from Streptomyces sp. MH816-AF15 as an inhibitor of cytidine 5'-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG): inositol transferase, caused a G1-phase accumulation in the cell cycle of small cell lung carcinomas. To investigate whether the cytostatic effect of inostamycin is restricted to lung carcinoma cell lines or applicable to other type of cells, we tested five oral squamous cell carcinoma (SCC) cell lines. Cell growth was suppressed in 62.5--125 ng/ml inostamycin in the culture medium in all oral cancer cell lines tested, with non-viable cells being <1%, indicating inostamycin is cytostatic on SCC cell lines. Decrease in cyclin D1 mRNA and protein expression due to the inostamycin treatment was accompanied by suppression of phosphorylated retinoblastoma susceptibility gene product (pRB-P) levels. Moreover, flow cytometric analysis showed that inostamycin induced an increase in G1/G0 cells (1.2--3.2 fold) over 24 h. These results suggest that inostamycin is a useful agent for tumour dormant cytostatic therapy for oral SCC.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Baba Y,Tsukuda M,Mochimatsu I,Furukawa S,Kagata H,Nagashima Y,Koshika S,Imoto M,Kato Ydoi
10.1006/cbir.2000.0706keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
613-20issue
7eissn
1065-6995issn
1095-8355pii
S1065-6995(00)90706-6journal_volume
25pub_type
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