Abstract:
:X-box-binding protein 1 (XBP-1) is a basic-region leucine zipper protein in the cyclic AMP response element binding protein/activating transcription factor (CREB/ATF) family of transcription factors involved in different cell-differentiation processes. We have investigated the expression of XBP-1 in differentiating MC3T3-E1 osteoblastic cells. Cultures were treated with ascorbic acid (AA) and beta-glycerophosphate (BGP) to induce differentiation. Under these conditions, the basal transcription of xbp-1 increases at day 2 following induction, peaks at day 5 and decreases thereafter. This result showed that xbp-1 gene is differentially expressed during MC3T3-E1 cell differentiation. Detection of XBP-1 by immunofluorescence at days 0 (control culture without AA and BGP), 8 and 21 showed that the protein has a major cytoplasmic perinuclear location. In addition, xbp-1 is transcriptionally upregulated by parathyroid hormone within 2.5 h of treatment and decreases thereafter.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Zambelli A,Mongiardini E,Villegas SN,Carri NG,Boot-Handford RP,Wallis GAdoi
10.1016/j.cellbi.2005.03.018keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
647-53issue
8eissn
1065-6995issn
1095-8355pii
S1065-6995(05)00083-1journal_volume
29pub_type
杂志文章abstract::Esophageal squamous cell carcinoma (ESCC) belongs to one of the most common malignant tumors worldwide and possesses high mortality. Long non-coding RNAs (lncRNAs) have been demonstrated to be essential biological participants in the progression of ESCC. On the basis of bio-informatics prediction, forkhead box P4 anti...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11338
更新日期:2020-07-01 00:00:00
abstract::Physical forces play a critical role in cell integrity and development, but little is known how cells convert mechanical signals into biochemical responses. This mini-review examines potential molecular mediators like integrins, focal adhesion proteins, and the cytoskeleton in the context of a complex cell structure. ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1006/cbir.2002.0857
更新日期:2002-01-01 00:00:00
abstract::DNA is constantly being damaged, either by endogenous or exogenous genotoxins. In that regard, DNA repair activities are essential for maintaining genomic stability and to life itself. Mutations in genes encoding DNA repair proteins cause severe human syndromes, but DNA repair defects have also been linked to several ...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10917
更新日期:2018-06-01 00:00:00
abstract::The effects of melanin preparations from black yeast fungi (BYF) on the proliferation and differentiation of normal cultivated human skin keratinocytes and embryonic pulmonary fibroblasts have been investigated. Melanin preparations in the range of 5-0.1 microg/ml were optimally active, with a more pronounced effect o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(02)00297-4
更新日期:2003-01-01 00:00:00
abstract::Lysosomal permeability to potassium ions is an important property of the organelle. Influence of the membrane physical state on the potassium ion permeability of isolated lysosomes was assessed by measuring the membrane potential with bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol and monitoring the lysosomal pro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2004.12.002
更新日期:2005-06-01 00:00:00
abstract::Tripartite motif protein 25 (TRIM25) expression was altered in various human cancers. Herein, we found that the expression of TRIM25 was elevated in hepatocellular carcinoma (HCC) tissues and cell lines. Knockdown of TRIM25 increased the sensitivity of HCC HepG2 cells to epirubicin (EPI), as indicated by reduced cell ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11346
更新日期:2020-07-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) are attached to each other in the bone marrow (BM) cavity and in in vitro cultures, and this adhesion has important physiological significance. We demonstrated that cell proliferation could be promoted when MSCs were co-cultured with EPCs, which was...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10579
更新日期:2016-04-01 00:00:00
abstract::Earlier, we have shown that spontaneously isolated non-pathogenic bacteria Serratia grimesii and Serratia proteamaculans invade eukaryotic cells, provided that they synthesize thermolysin-like metalloproteases ECP32/grimelysin or protealysin characterized by high specificity towards actin. To address the question of w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100314
更新日期:2011-02-01 00:00:00
abstract::Klotho beta (Klb), a single-pass transmembrane protein, has been described as a co-receptor for endocrine FGFs, such as FGF15/19 and FGF21, to regulate critical metabolic processes in multiple organs and tissues in adult mice. However, its function during early embryonic development remains largely unknown. In this pa...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11059
更新日期:2019-02-01 00:00:00
abstract::Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temp...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10360
更新日期:2015-02-01 00:00:00
abstract::Corticotropin-releasing hormone, which is the predominant regulator of neuroendocrine responses to stress, attenuates inflammation through stimulation of glucocorticoid release. Enhanced corticotropin-releasing hormone expression has been detected in inflammatory cells of the vascular endothelium, where it acts as a l...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11188
更新日期:2019-05-29 00:00:00
abstract::To examine cytokine production in response to RSV infection, we assessed the levels of 29 cytokines released from RSV-infected human foetal lung fibroblasts. We also examined the relationships between the effects of fluticasone propionate and various signalling pathways in the cells. Twenty-four hours after infection ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10044
更新日期:2013-04-01 00:00:00
abstract::A convenient means of measuring apoptosis is described using an automated analyzer (MF-1; Sysmex), which normally provides rapid measurement of malarial parasites. By means of this MF-1 procedure, apoptotic cells exhibited characteristic changes of light scatter (size) and fluorescence (DNA content) relating to cell s...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0560
更新日期:2000-01-01 00:00:00
abstract::Here we report a homologue of the apolipoprotein D gene (AmphiApoD) in amphioxus, Branchiostoma belcheri tsingtauense, the first such finding in a basal chordate cephalochordate. The main features of the protein predicted from AmphiApoD are characteristic of the apolipoprotein D. Phylogenetic analysis places AmphiApoD...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.09.011
更新日期:2007-01-01 00:00:00
abstract::Transforming growth factor-β (TGF-β) is a potent inhibitor of the growth of normal mammary epithelial cells, and has a pleiotropic, context-dependent, concentration-dependent action. We found attenuation of TGF-β signalling in mammary adenoma carcinoma cells. Phosphorylation at the linker site of Smad2 occurred in a c...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10061
更新日期:2013-05-01 00:00:00
abstract::Cell proliferation rates of diagnostic marrow aspirate cells of 21 children with Acute Lymphoblastic Leukaemia and 16 controls were compared using immunocytochemical labelling of PCNA and Ki-67 antigen as assessed by Confocal Laser Scanning Microscopy. The results showed an unexpected, highly significant degree of dis...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1123
更新日期:1994-09-01 00:00:00
abstract::Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells that possesses practical and ethical advantages. We previously reported a novel UCB-derived adult stem cells which we termed umbilical cord blood-derived multipotent progenitor cells' (MPCs). MPCs were capable of differentiating into functional ne...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.07.017
更新日期:2008-10-01 00:00:00
abstract::Disconnected interacting protein 2 (DIP2) is a highly conserved protein family among invertebrates and vertebrates, but its function remains unclear. In this paper, we summarized the conservation of gene sequences and protein domains of DIP2 family members and predicted that they may have a similar functional role in ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11106
更新日期:2019-04-01 00:00:00
abstract::Continuous variation in the direction of the gravity vector leads to various cellular responses including modulation of gene expression. Complementary DNA (cDNA) array analyses are available to observe the variation of gene expression under different conditions. In this study, expression levels of 588 representative g...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0833
更新日期:2002-01-01 00:00:00
abstract::Adipose tissue is a potential site of retinoic acid (RA) action, but its physiological significance remains to be clarified. We have examined the effect of all-trans retinoic acid (ATRA) on growth and differentiation of preadipocytes, and on adipokine gene expression in mature adipocytes using human preadipocyte cell ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10005
更新日期:2013-01-01 00:00:00
abstract::Circulating endothelial progenitor cells (EPCs) play a key role in restoring endothelial function and enhancing angiogenesis. However, the effects of low-dose aspirin on circulating EPCs are not well known. We investigated the effects of low-dose aspirin on EPC migration, adhesion, senescence, proliferation, apoptosis...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.03.004
更新日期:2008-07-01 00:00:00
abstract::The expression and immunohistochemical localization of galectin-3, a beta-galactoside-binding protein, was studied in several mouse tissues. Galectin-3 expression was low in the cerebrum, heart, and pancreas, and moderate in the liver, ileum, kidney, and adrenal gland. High expression of galectin-3 was found in the lu...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.11.036
更新日期:2007-07-01 00:00:00
abstract::Calcium loss and muscle atrophy are two of the main metabolic changes experienced by astronauts and crew members during exposure to microgravity in space. Calcium and cytoskeletal events were investigated within sea urchin embryos which were cultured in space under both microgravity and 1 g conditions. Embryos were fi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0371
更新日期:1999-01-01 00:00:00
abstract::Dexmedetomidine (Dex) protects different cell types during hypoxia or ischemia-reperfusion injury by inhibiting cell apoptosis. However, the underlying mechanism and its impact on hepatic ischemia reperfusion injury are still not known. In this study, we established a model of oxygen-glucose deprivation/reperfusion (O...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10996
更新日期:2018-09-01 00:00:00
abstract::The interaction of NO and O2- free radicals generated from PMA (phorbol myristate acetate)-stimulated PMN (polymorphonuclear leukocytes) was studied by a nitroxide spin trap, DMPO (5,5-dimethyl-1-pyrroline-1-oxide). It was found that addition of L-arginine to the system would significantly decrease the trapped O2- by ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0039
更新日期:1996-05-01 00:00:00
abstract::Treatment of cultured rat osteoblasts with staurosporine caused a rapid outgrowth of long slender cellular processes and the formation of stellate cells. The number of stellate cells increased with higher concentrations of and longer incubation with staurosporine. Scanning electron microscopy (SEM) revealed that the s...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1007
更新日期:1993-01-01 00:00:00
abstract::Although several species of Tetrahymena are often described as histophagous and opportunistic pathogens of fish, little is known about ciliate/fish cell interactions, but one approach for studying these is in vitro with cell lines. In this study, T. thermophila, B1975 (wild type) and NP1 (temperature sensitive mutant ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10310
更新日期:2014-10-01 00:00:00
abstract::We investigated the effect of triptolide (TP) on spinal cord injury (SCI), and its underlying mechanism. Following the establishment of the SCI model using YFP H-line transgenic mice, TP was intraperitoneally injected at a dose of 0.2 mg/kg once daily for 7 days. Behavioral tests, Nissl staining, and hematoxylin-eosin...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11273
更新日期:2020-03-01 00:00:00
abstract::The introduction of four key transcriptional factors (CRX, RAX, NEURO-D, OTX2) allows the direct differentiation of fibroblasts to retinal photoreceptor cells. This reprogramming was achieved with a combination of mono-cistronic viruses. Although the combination of mono-cistronic viruses was useful, a relatively high ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10942
更新日期:2018-05-01 00:00:00
abstract::Wnt signalling regulates cell proliferation and cell fate determination during embryogenesis. However, little is known about the developmental role of one Wnt family member, Wnt-3, during avian development. To investigate the possible functions of Wnt-3, its expression pattern was determined using whole-mount in situ ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090133
更新日期:2010-07-01 00:00:00