Transcription factor XBP-1 is expressed during osteoblast differentiation and is transcriptionally regulated by parathyroid hormone (PTH).

Abstract:

:X-box-binding protein 1 (XBP-1) is a basic-region leucine zipper protein in the cyclic AMP response element binding protein/activating transcription factor (CREB/ATF) family of transcription factors involved in different cell-differentiation processes. We have investigated the expression of XBP-1 in differentiating MC3T3-E1 osteoblastic cells. Cultures were treated with ascorbic acid (AA) and beta-glycerophosphate (BGP) to induce differentiation. Under these conditions, the basal transcription of xbp-1 increases at day 2 following induction, peaks at day 5 and decreases thereafter. This result showed that xbp-1 gene is differentially expressed during MC3T3-E1 cell differentiation. Detection of XBP-1 by immunofluorescence at days 0 (control culture without AA and BGP), 8 and 21 showed that the protein has a major cytoplasmic perinuclear location. In addition, xbp-1 is transcriptionally upregulated by parathyroid hormone within 2.5 h of treatment and decreases thereafter.

journal_name

Cell Biol Int

authors

Zambelli A,Mongiardini E,Villegas SN,Carri NG,Boot-Handford RP,Wallis GA

doi

10.1016/j.cellbi.2005.03.018

keywords:

subject

Has Abstract

pub_date

2005-08-01 00:00:00

pages

647-53

issue

8

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(05)00083-1

journal_volume

29

pub_type

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