Prostate cancer cells modulate the differentiation of THP-1 cells in response to etoposide and TLR agonists treatments.

Abstract:

:The aim of this study was to determine the polarization of macrophages in the tumor microenvironment, as well as the effect of soluble factors secreted from these polarized macrophages on etoposide-induced cancer cell apoptosis. We investigated the effect of soluble factors secreted from the supernatant of PC3 cells treated with TLR4 and TLR8 agonists, and etoposide on macrophage polarization at the protein level through flow cytometry and enzyme-linked immunosorbent assay. We further explored the cell cycle distribution and phagocytic activity of THP-1 cells by flow cytometry. To imitate the relationship between cancer cells and tumor-associated macrophages (TAMs), we cocultured macrophages with etoposide-treated PC3 cells. After the incubation, the apoptosis in cancer cells was assessed through FACS analysis and by annexin V and PI staining. Our results demonstrate that protein expression of M1 and M2 markers confirmed the upregulation of M1 markers upon etoposide treatment, and mixed M1/M2 phenotype upon treatment with TLR agonists-treated PC3 supernatant. In coculture methods, our results demonstrate that the apoptosis of etoposide-treated cancer cells increases in the presence of M0 macrophages and THP-1 cells incubated with the supernatant of TLR4 agonists-treated PC3 cells. These results indicate clear protective effects of M0 macrophages and THP-1 cells incubated with the supernatant of PC3 cells treated with TLR4 agonists (THP-1 + SUP +  TLR4a) on etoposide-induced cancer cell apoptosis.

journal_name

Cell Biol Int

authors

Malekghasemi S,Majidi J,Baradaran B,Aghebati-Maleki L

doi

10.1002/cbin.11410

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

2031-2041

issue

10

eissn

1065-6995

issn

1095-8355

journal_volume

44

pub_type

杂志文章
  • LncRNA-XIST promotes the oxidative stress-induced migration, invasion, and epithelial-to-mesenchymal transition of osteosarcoma cancer cells through miR-153-SNAI1 axis.

    abstract::Osteosarcoma (OS) is the most common type of primary bone tumor that exhibits invasive growth and long-distance organ metastasis. Thus, investigating the specifically targeted therapeutic agents against metastatic osteosarcoma depends on understanding the molecular mechanisms. The long noncoding RNAs (lncRNA) XIST (X-...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11405

    authors: Wen JF,Jiang YQ,Li C,Dai XK,Wu T,Yin WZ

    更新日期:2020-10-01 00:00:00

  • Calmodulin-like skin protein suppresses the increase in senescence-associated β-galactosidase induced by hydrogen peroxide or ultraviolet irradiation in keratinocytes.

    abstract::Calmodulin-like skin protein (CLSP) is a secreted peptide that is produced by skin keratinocytes and some related epithelial cells. It has previously been shown that CLSP is recruited via the bloodstream into the central nervous system where it likely exerts a neuroprotective effect against toxicity related to Alzheim...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11159

    authors: Takahara Y,Miyachi N,Nawa M,Matsuoka M

    更新日期:2019-07-01 00:00:00

  • High resolution scanning force microscopy of cardiac myocytes.

    abstract::The advent of scanning probe microscopy has introduced a powerful new method of probing the structural features of biological specimens. In this study, high resolution atomic force microscopy micrographs of single, isolated, cardiac myocytes are presented. Significantly, our images show not only the features to be exp...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0813

    authors: Davis JJ,Hill HA,Powell T

    更新日期:2001-01-01 00:00:00

  • The possible double-edged sword effects of vitamin D on COVID-19: A hypothesis.

    abstract::Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) gains access to host cells by attaching to angiotensin-converting enzyme 2 (ACE2). Vitamin D (VitD) can upregulate ACE2 and has an antagonistic effect on Renin, which exerts a vasodilatation and anti-inflammatory effect against coronavirus disease (COVID-19)...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.11469

    authors: Shojaeefar E,Malih N,Rezaei N

    更新日期:2021-01-01 00:00:00

  • Long Non-coding RNA NEAT1 Drives the Development of Polycystic Ovary Syndrome via Sponging Multiple MicroRNAs.

    abstract::A line of evidences have validated that multiple microRNAs (including miR-16, miR-483 and miR-324-3p) were abnormally expressed in granulosa cells, which may be closely related with the pathogenesis of polycystic ovary syndrome (PCOS). Long-chain non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11349

    authors: Sang X,Zhang Y

    更新日期:2020-03-28 00:00:00

  • Detection of potassium currents and regulation of multidrug resistance by potassium channels in human gastric cancer cells.

    abstract::The present study aimed to investigate the potassium currents and further explore the role of potassium channels in drug response of gastric cancer cells. By patch-clamp technique, potassium currents of human gastric cancer cell SGC7901 were recorded in the mode of voltage clamp. Both 4-aminopyridine (4-AP) and tetrae...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.01.008

    authors: Han Y,Shi Y,Han Z,Sun L,Fan D

    更新日期:2007-07-01 00:00:00

  • Peroxynitrite activates K+-Cl- cotransport in human erythrocytes.

    abstract::Peroxynitrite was found to induce the release of K+ via the Na+/Cl- cotransport system, as do other oxidants. Since peroxynitrite is formed in vivo, its presence could contribute to a pathological dehydration of red blood cells. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0778

    authors: Grzelak A,Mazur J,Bartosz G

    更新日期:2001-01-01 00:00:00

  • Murine osteoclastogenesis suppression using conditioned media produced by melanoma or activated and non-activated Jurkat-E6 cells.

    abstract::Conditioned medium (CM) (cell secretome) is a cocktail of growth factors, cytokines, and other soluble mediators secreted by cells into a culture medium. These growth factors are fundamental in many cellular processes such as cell growth, differentiation, and others and the composition of these factors is individual f...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11317

    authors: Mambelli-Lisboa NC,Frare EO,da Costa-Neves A,Prieto da Silva ÁRB,Kerkis I

    更新日期:2020-05-01 00:00:00

  • Palmitate exerts opposite effects on proliferation and differentiation of skeletal myoblasts.

    abstract::The purpose of the study was to examine mechanisms controlling cell cycle progression/arrest and differentiation of mouse C2C12 myoblasts exposed to long-chain saturated fatty acid salt, palmitate. Treatment of proliferating myoblasts with palmitate (0.1 mmol/l) markedly decreased myoblast number. Cyclin A and cyclin ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10477

    authors: Grabiec K,Milewska M,Błaszczyk M,Gajewska M,Grzelkowska-Kowalczyk K

    更新日期:2015-09-01 00:00:00

  • Histamine promotes the expression of receptors TLR2 and TLR4 and amplifies sensitivity to lipopolysaccharide and lipoteichoic acid treatment in human gingival fibroblasts.

    abstract::The vasoactive hydrophilic amine histamine is the most important molecule released by mast cells. However, histamine's role in activating intracellular responses in HGFs (human gingival fibroblasts) has not been evaluated to date. In the present study, we investigated the effect of histamine and of Gram-negative [LPS ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20100624

    authors: Gutiérrez-Venegas G,Cruz-Arrieta S,Villeda-Navarro M,Méndez-Mejía JA

    更新日期:2011-10-01 00:00:00

  • Muscarinic acetylcholine receptors involved in the regulation of neural stem cell proliferation and differentiation in vitro.

    abstract::Neural stem cells (NSCs) are currently considered powerful candidates for cell therapy in neurodegenerative disorders such as Parkinson's disease. However, it is not known when and how NSCs begin to differentiate functionally. Recent reports suggest that classical neurotransmitters such as acetylcholine (Ach) are invo...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2003.10.005

    authors: Zhou C,Wen ZX,Shi DM,Xie ZP

    更新日期:2004-01-01 00:00:00

  • Nuclear co-repressor (NCoR) is required to maintain insulin sensitivity in C2 C12 myotubes.

    abstract::Nuclear co-repressor (NCoR) regulates peripheral insulin sensitivity; however, its role in modulating insulin sensitivity in skeletal muscle remains elusive. Present study investigated protein expression and effect of NCoR on insulin sensitivity in murine skeletal muscle cell line C2 C12 . Myotubes as compared to myob...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10711

    authors: Choudhary AK,Dey CS

    更新日期:2017-02-01 00:00:00

  • K562 cells display different vulnerability to H₂O₂ induced oxidative stress in differing cell cycle phases.

    abstract::Oxidative stress can be defined as the increase of oxidizing agents like reactive oxygen and nitrogen species, or the imbalance between the antioxidative defense mechanism and oxidants. Cell cycle checkpoint response can be defined as the arrest of the cell cycle functioning after damaging chemical exposure. This temp...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10360

    authors: Akcakaya H,Dal F,Tok S,Cinar SA,Nurten R

    更新日期:2015-02-01 00:00:00

  • High-level expression and purification of soluble bioactive recombinant human heparin-binding epidermal growth factor in Escherichia coli.

    abstract::Heparin-binding epidermal growth factor (HB-EGF) is a member of highly conserved superfamily of proteins that has potential mitogenic activity and stimulates differentiation and migration of various cell types. Since HB-EGF has three intra-molecular disulfide bonds, a high expression pattern of active HB-EGF in an E. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10454

    authors: Khalili M,Soleyman MR,Baazm M,Beyer C

    更新日期:2015-07-01 00:00:00

  • Intranuclear network of 3-5 nm and 8-10 nm fibers in EL-4 lymphoma cells.

    abstract::While much evidence indicates a high degree of spatial organization in the nucleus, the underlying molecular structures that support it remain poorly characterized. By extracting with high concentrations of RNase A in a modification of the sequential extraction protocol of Penman, we have identified a novel intranucle...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1997.0149

    authors: Padros MR,Giles DM,Suarez MD

    更新日期:1997-06-01 00:00:00

  • Evaluation of STAT3 decoy oligodeoxynucleotides' synergistic effects on radiation and/or chemotherapy in metastatic breast cancer cell line.

    abstract::Resistance to radiotherapy and chemotherapy has been a major problem of conventional cancer therapies, which consequently leads to cancer relapse and cancer-related death. It is known that cancer stem cells (CSCs) play a key role in therapy resistance and CSC-based targeted therapies have been considered as a powerful...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11456

    authors: Johari B,Rahmati M,Nasehi L,Mortazavi Y,Faghfoori MH,Rezaeejam H

    更新日期:2020-12-01 00:00:00

  • Comparison of the endogenous IK currents in rat hippocampal neurons and cloned Kv2.1 channels in CHO cells.

    abstract::The Kv2.1 potassium channel is a principal component of the delayed rectifier I(K) current in the pyramidal neurons of cortex and hippocampus. We used whole-cell patch-clamp recording techniques to systemically compare the electrophysiological properties between the native neuronal I(K) current of cultured rat hippoca...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.08.019

    authors: Liu M,Gong B,Qi Z

    更新日期:2008-12-01 00:00:00

  • Cytotoxicity of daunorubicin in trisomic (+21) human fibroblasts: relation to drug uptake and cell membrane fluidity.

    abstract::The influence of daunorubicin (DNR) on survival of human normal (S-126) and trisomic, with respect to chromosome 21 (T-164; S-240), skin fibroblasts and some parameters related to it, such as intracellular drug accumulation, distribution and interaction with cell membrane, were studied. The in vitro growth-inhibition ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0583

    authors: Przybylska M,Koceva-Chyla A,Rózga B,Józwiak Z

    更新日期:2001-01-01 00:00:00

  • Tyrosine phosphorylation is involved in Ca(2+)entry in human gingival fibroblasts.

    abstract::Bradykinin (1 microM) and histamine (100 microM) evoked an initial transient increase and a subsequent sustained increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded human gingival fibroblasts, which may be attributed to Ca(2+) release from intracellular stores and Ca(2+) entry from extracellu...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/s1065-6995(03)00125-2

    authors: Ogata Y,Nakao S,Shimizu E,Matsuda-Honjyo Y,Yamazaki M,Furuyama S,Sugiya H

    更新日期:2003-01-01 00:00:00

  • Enrichment and characterization of mouse putative epidermal stem cells.

    abstract::Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal ste...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.04.007

    authors: Zhou JX,Jia LW,Yang YJ,Peng S,Cao YJ,Duan EK

    更新日期:2004-01-01 00:00:00

  • Study of the protective effect on damaged intestinal epithelial cells of rat multilineage-differentiating stress-enduring (Muse) cells.

    abstract::In this study, we determined whether multilineage-differentiating stress-enduring (Muse) cells exist in rat bone marrow and elucidated their effects on protection against the injury of intestinal epithelial cells associated with inflammation. Rat Muse cells were separated from bone marrow mesenchymal stem cells (BMMSC...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11255

    authors: Sun D,Yang L,Cao H,Shen ZY,Song HL

    更新日期:2020-02-01 00:00:00

  • Prevention of intracellular oxidation in yeast: the role of vitamin E analogue, Trolox (6-hydroxy-2,5,7,8-tetramethylkroman-2-carboxyl acid).

    abstract::Reactive oxygen species (ROS) are not only generated in conditions of cellular stress but are also constitutively produced in most cell types by specific metabolic processes. This research focused on a potential antioxidant Trolox (model compound for alpha-tocopherol), with the aim to establish exact mechanisms of Tro...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2004.11.010

    authors: Raspor P,Plesnicar S,Gazdag Z,Pesti M,Miklavcic M,Lah B,Logar-Marinsek R,Poljsak B

    更新日期:2005-01-01 00:00:00

  • Genistein protects ovarian granulosa cells from oxidative stress via cAMP-PKA signaling.

    abstract::Genistein is an isoflavone that has estrogen (E2 )-like activity and is beneficial for follicular development, but little is known regarding its function in oxidative stress (OS)-mediated granulosa cell (GC) injury. Here, we found that after exposure to H2 O2 , Genistein weakened the elevated levels of intracellular r...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11244

    authors: Luo M,Yang ZQ,Huang JC,Wang YS,Guo B,Yue ZP

    更新日期:2020-02-01 00:00:00

  • Chlamydia pneumoniae induces macrophage-derived foam cell formation via PPAR alpha and PPAR gamma-dependent pathways.

    abstract::In the presence of low density lipoprotein (LDL), Chlamydia pneumoniae induces macrophage-derived foam cell formation, a typical pathological feature of early atherosclerosis. However, its mechanism has not been fully understood. Peroxisome proliferator-activated receptors (PPARs) are key regulators of macrophage lipi...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.12.002

    authors: Mei CL,He P,Cheng B,Liu W,Wang YF,Wan JJ

    更新日期:2009-03-01 00:00:00

  • Identification of circulating miRNA involved in meat yield of Korean cattle.

    abstract::Cattle plays an important role in providing essential nutrients through meat production. Thus, we focused on epigenetic factors associated with meat yield. To investigate circulating miRNAs that are involved with meat yield and connect biofluids and longissimus dorsi (LD) muscle in Korean cattle, we performed analyses...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10776

    authors: Lee S,Park SJ,Cheong JK,Ko JY,Bong J,Baik M

    更新日期:2017-07-01 00:00:00

  • Detection of intranucleolar chromatin using an ultrastructural immunolabelling technique.

    abstract::The intranucleolar chromatin has been detected by feeding HeLa cells with bromodeoxyuridine and then using an immunocytochemical technique for its detection at the ultrastructural level. Labelling was mainly observed on the dense fibrillar component (DFC), which surrounds the fibrillar centres (FC). Labelling could al...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1998.0265

    authors: Ghosh S,Paweletz N

    更新日期:1998-01-01 00:00:00

  • Polyamines and terminal deoxynucleotidyl transferase expression in KM 3 pre-B cell line during phorbol ester induced differentiation.

    abstract::The aliphatic polyamines, putrescine, spermine and spermidine belong to a category of molecules implicated in DNA replication. Their synthesis is strongly activated during the G1 period and they have been implicated in the regulation of cell proliferation and differentiation. Terminal transferase is a DNA polymerase p...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1995.1016

    authors: Stabellini G,Rapino M,Di Primio R,Trubiani O

    更新日期:1995-10-01 00:00:00

  • Tissues formed during distraction osteogenesis in the rabbit are determined by the distraction rate: localization of the cells that express the mRNAs and the distribution of types I and II collagens.

    abstract::An experimental model of leg lengthening was used to study the morphology of, the collagenous proteins present, and the collagen genes expressed in the regenerating tissue following 20% lengthening at four different distraction rates. At a distraction rate of 0.3 mm/day (8 weeks distraction), the regenerate consists o...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1999.0449

    authors: Li G,Virdi AS,Ashhurst DE,Simpson AH,Triffitt JT

    更新日期:2000-01-01 00:00:00

  • Rapamycin improves insulin resistance and hepatic steatosis in type 2 diabetes rats through activation of autophagy.

    abstract::Insulin resistance (IR) is a hallmark of type 2 diabetes mellitus (T2DM). This study aimed to explore the effects of rapamycin, a specific inhibitor of kinase mammalian target of rapamycin (mTOR), on IR in T2DM rats, and to validate whether the underlying mechanism was associated with autophagy. In this study, the mod...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11015

    authors: Zhou W,Ye S

    更新日期:2018-09-01 00:00:00

  • High-glucose concentration aggravates TNF-alpha-induced cell viability reduction in human CD146-positive periodontal ligament cells via TNFR-1 gene demethylation.

    abstract::Periodontitis is a chronic inflammatory disease that results in the destruction of periodontal soft tissue and the resorption of alveolar bone. Evidence indicates that in diabetic patients, hyperglycemia suppresses periodontal ligament stem cell (PDLSC) functions and leads to difficulties in periodontal repair. The pr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11445

    authors: Luo H,Zhu W,Mo W,Liang M

    更新日期:2020-12-01 00:00:00