Abstract:
:Oral squamous cell carcinoma (OSCC) is a serious global health problem. Recently, accumulating microRNA (miRNA) has emerged as crucial players in the development and progression of carcinomas including OSCC. Our study aimed to further investigate the roles of miR-140-5p in OSCC tumorigenesis and related molecular basis. In this study, OSCC tissues and adjacent normal tissues were isolated from 34 OSCC patients who suffered from surgical resection at our hospital. MiR-140-5p level was measured by reverse-transcription quantitative polymerase chain reaction assay. p21-activated kinase 4 (PAK4) protein level was determined by western blot assay in OSCC cells at 48 h posttransfection or OSCC xenograft tumors at day 35 after OSCC cell injection. The cell proliferative ability was assessed by cell counting kit-8 assay in OSCC cells at 0, 24, 48, 72 h after transfection. Cell apoptosis and cell-cycle analysis was conducted using a flow cytometry in OSCC cells at 48 h after transfection. The interaction between miR-140-5p and PAK4 3'-untranslated region was tested by bioinformatics analysis and luciferase reporter assay in OSCC cells at 48 h after transfection. Mouse xenograft models of OSCC were established to examine the influence of miR-140-5p on OSCC tumorigenesis in vivo during 35 days after OSCC cell injection. Our data showed that miR-140-5p expression was notably downregulated in OSCC tissues and cell lines. MiR-140-5p inhibited the expression of PAK4 by direct interaction in OSCC cells. Functional analysis disclosed that miR-140-5p overexpression or PAK4 knockdown suppressed cell proliferation, promoted cell apoptosis, and induced cell-cycle arrest in OSCC. Moreover, PAK4 upregulation rescued the detrimental effects of miR-140-5p on cell proliferation and cell-cycle progression and hampered cell apoptosis induced by miR-140-5p in OSCC. In vivo experiments demonstrated that miR-140-5p overexpression suppressed the growth of OSCC xenograft tumors by downregulating PAK4. In conclusion, our data revealed miR-140-5p suppressed OSCC tumorigenesis by targeting PAK4 in vitro and in vivo, deepening our understanding on the function and molecular basis of miR-140-5p in the development of OSCC.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Peng M,Pang Cdoi
10.1002/cbin.11213subject
Has Abstractpub_date
2019-08-08 00:00:00eissn
1065-6995issn
1095-8355pub_type
杂志文章abstract::We describe a method to hold living cells in place that ordinarily do not adhere to glass coverslips. The method, developed for insect spermatocytes but with application to other cell types, consists of embedding cells in a fibrin clot that forms after the enzyme thrombin cleaves the blood protein fibrinogen. The meth...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.04.010
更新日期:2005-09-01 00:00:00
abstract::Normal human bone marrow stroma cells include stem cells for both haemopoietic and osteochondrogenic lineages and express both bone morphogenetic protein (BMP) type I and type II receptors. As a member of the TGF-beta super-family, BMP-6 binds to both BMP type I and type II receptors and is involved in the development...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0662
更新日期:2001-01-01 00:00:00
abstract::The induction and maintenance of immunologic tolerance at the feto-maternal interface is necessary for a successful pregnancy. The most accepted hypothesis for the mechanism underlying this tolerance is that pregnancy-induced foetal antigen-specific maternal T regulatory (T(reg) ) cells mediate maternal tolerance to t...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10023
更新日期:2013-02-01 00:00:00
abstract::Fibrinogen participates in important cellular physiological processes, such as cell adhesion and blood clotting. Although the primary and secondary structures of fibrinogen are known, its tertiary structure is yet to be determined. In attempts to understand the tertiary structure of this important hydrated cellular an...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1997.0216
更新日期:1997-11-01 00:00:00
abstract::The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were search...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0099
更新日期:1996-11-01 00:00:00
abstract::A line of evidences have validated that multiple microRNAs (including miR-16, miR-483 and miR-324-3p) were abnormally expressed in granulosa cells, which may be closely related with the pathogenesis of polycystic ovary syndrome (PCOS). Long-chain non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11349
更新日期:2020-03-28 00:00:00
abstract::The advent of scanning probe microscopy has introduced a powerful new method of probing the structural features of biological specimens. In this study, high resolution atomic force microscopy micrographs of single, isolated, cardiac myocytes are presented. Significantly, our images show not only the features to be exp...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0813
更新日期:2001-01-01 00:00:00
abstract::We have investigated constitutive endocytosis in internodal cells of the characean green algae. The endocytic tracer FM1-43 accumulated in distinct plasma membrane domains that are probably enriched in sterol-like substances. Internalization of the dye was active but independent of an intact actin or microtubule cytos...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.11.002
更新日期:2008-05-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) plays a key role in formation of pleural effusions and in tumorigenesis and progression of malignant mesothelioma. Mesothelial cells (MC) express the viral receptors Toll-like receptor 3 (TLR3), RIG-I and MDA5. Activation of these receptors by viral RNA exemplified by poly(I:C...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.10.011
更新日期:2009-02-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) exists low efficiency to trans-differentiate into other germinal layer cell types. One key issue is to discover the effect of important factor on MSCs differentiation abiltiy. In this study, we investigated the role and mechanism of epiregulin (EREG) on the osteogenic differentiation and ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11305
更新日期:2020-04-01 00:00:00
abstract::Recently, we reported that human PDB (placental decidua basalis) is an excellent source of MSCs (mesenchymal stem cells), meanwhile, PDB-MSCs could survive under hypoxia and serum deprivation. Herein, we investigated the proliferation, clonogentic efficiency, phenotypes, metabolic activity and cytokines secretion of P...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20090044
更新日期:2010-01-27 00:00:00
abstract::Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) gains access to host cells by attaching to angiotensin-converting enzyme 2 (ACE2). Vitamin D (VitD) can upregulate ACE2 and has an antagonistic effect on Renin, which exerts a vasodilatation and anti-inflammatory effect against coronavirus disease (COVID-19)...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.11469
更新日期:2021-01-01 00:00:00
abstract::Emerging evidence suggests that miR-143 plays an important role in the regulation of tumor sensitivity to chemotherapeutic agents. The study explores the underlying mechanism of miR-143 in reversing cisplatin resistance in ovarian cancer. The cisplatin-resistant ovarian cancer cell line A2780/CDDP was induced and esta...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11486
更新日期:2021-01-01 00:00:00
abstract::Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC-R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC-R1/E and EBs were allowed to attach to the bottoms of normal cell-culturing plate and grow. Then, different...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10380
更新日期:2014-12-01 00:00:00
abstract::Hepatic oval cells are likely to be activated during advanced stage of liver fibrosis to reconstruct damaged hepatic tissue. However, their scarcity, difficulties in isolation, and in vitro expansion hampered their transplantation in fibrotic liver. This study was aimed to investigate the repair potential of in vitro ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10699
更新日期:2017-01-01 00:00:00
abstract::The synthesis of proteins from salt-tolerant Brassica oleracea L. var. botrytis L. subvar. cauliflora (Gars.) DC. (cauliflower) cell cultures is modified in relation to controls in several features. There are nine newly induced polypeptides in tolerant cultures (absent in control conditions). Some of them are only pre...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1146
更新日期:1993-09-01 00:00:00
abstract::Gelatinase activity and inhibitory activity against collagenase were measured in serum-free medium conditioned by murine colonic carcinoma cells with different spontaneous metastatic potentials to the lung. The medium conditioned with poorly metastatic NM11 cells gave higher inhibitory activity than that conditioned w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1057
更新日期:1994-03-01 00:00:00
abstract::In 3D cultured cell systems, the cells form 3D spheroids that mimic cancer cell spheroids in vivo. Cancer cells form cell spheroids as they grow. The in vivo spheroids do not contain a vascular network; therefore, oxygen and nutrition supplies are insufficient. Specifically, the cells in the core region of the cluster...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10656
更新日期:2016-11-01 00:00:00
abstract::In the adult brain, neural stem cells from the subgranular zone (SGZ) of the hippocampus and the subventricular zone (SVZ) of the cortex progress through the following five developmental stages: radial glia-like cells, neural progenitor cells, neuroblasts, immature neurons, and mature neurons. These developmental stag...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10569
更新日期:2016-04-01 00:00:00
abstract::Periodontitis is a chronic inflammatory disease that results in the destruction of periodontal soft tissue and the resorption of alveolar bone. Evidence indicates that in diabetic patients, hyperglycemia suppresses periodontal ligament stem cell (PDLSC) functions and leads to difficulties in periodontal repair. The pr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11445
更新日期:2020-12-01 00:00:00
abstract::Fndc5 has been recently recognized as a myokine which could be cleaved and secreted into blood stream. It is termed as irisin with an important role in thermogenesis and energy homeostasis. Increased expression of Fndc5 has been reported upon retinoic acid treatment during neural differentiation and its knockdown decr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10427
更新日期:2015-05-01 00:00:00
abstract::δ-Catenin and β-catenin belong to different subfamilies of armadillo proteins but share some common binding partners, such as E-cadherin. This is the first study that demonstrated a novel common binding partner for δ-catenin and β-catenin, lymphoid enhancer factor-1 (LEF-1). We found that the N-terminus of δ-catenin (...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10465
更新日期:2015-08-01 00:00:00
abstract::Increasing potassium chloride concentration from 0 to 100 mM and magnesium chloride from 0 to 2 mM show a parallel rate increase in polymerizing actin, whereas increasing calcium chloride concentration from 0 to 0.2 mM decreases the rate of polymerizing actin. The presence of alpha-actinin has little influence on the ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0898
更新日期:2002-01-01 00:00:00
abstract::Stem/progenitor cells hold promise for alleviating/curing type 1 diabetes due to the capacity to differentiate into functional insulin-producing cells. The current study aims to assess the differentiation potential of human pancreatic IPCs (islet-derived progenitor cells). IPCs were derived from four human donors and ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100152
更新日期:2011-05-01 00:00:00
abstract::Mammalian cells in vivo frequently express primary cilia. Although some fully differentiated cell types rarely, if ever, express them, most do, indicating that they are regular cell organelles. Their expression can also be explored in vitro, where conditions--physical and chemical, intrinsic and extrinsic--permit expe...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1006/cbir.1996.0011
更新日期:1996-01-01 00:00:00
abstract::We studied the genotoxic potential of a carcinogen in the human hepatoma cell line, HepG2, and in primary rat hepatocyte culture. HepG2 is a well differentiated human hepatoblastoma cell line with biotransforming capacity. Rat hepatocytes were obtained by the standard two-step in situ perfusion technique. Following be...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1083
更新日期:1993-04-01 00:00:00
abstract::The aim of this study was to determine the polarization of macrophages in the tumor microenvironment, as well as the effect of soluble factors secreted from these polarized macrophages on etoposide-induced cancer cell apoptosis. We investigated the effect of soluble factors secreted from the supernatant of PC3 cells t...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11410
更新日期:2020-10-01 00:00:00
abstract::CD25+Treg cells (CD4+CD25+Foxp3+ regulatory T cells) play a central role in the maintenance of peripheral self-tolerance and immune homoeostasis. A previous study showed that CD25+Treg cells suppressed the differentiation and function of Th1 cells in vivo and in vitro. However, the mechanism of suppressing Th1 cell di...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100528
更新日期:2011-07-01 00:00:00
abstract::The single-minded 2 gene (Sim2) plays a crucial role in the mental retardation of Down Syndrome (DS). To explore how Sim2 influences spatial memory, a DNA plasmid-encoding mouse Sim2 (mSim2) wrapped with liposome was bilaterally injected into the hippocampus of rats. The effect of overexpressing mSim2 on spatial learn...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.07.003
更新日期:2006-10-01 00:00:00
abstract::Silicon dioxide induces acute injury and chronic pulmonary fibrosis. International Agency for Research on Cancer (IARC) listed it as a human carcinogen in 1996. However, the molecular mechanisms to induce cancer are not understood yet. The content of poly (ADP-ribose) polymerases (PARP) mRNA and protein in Hela cells ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2009.04.010
更新日期:2009-07-01 00:00:00