Abstract:
:Mitomycin C (MMC) treatment has been used to arrest cell proliferation but not much is known about the effect of MMC on human foreskin fibroblasts (HFF) used as feeders for human embryonic stem cells (hESC). We tested the ability of MMC to stop the proliferation of HFF and to induce apoptosis. MMC inhibited the proliferation of HFF at 10 microg/ml over 2.5h of MMC treatment showing a decrease in the proliferation index measured by Ki-67 and S and G2/M phases related to active HFF. A low percentage of cells showed necrotic or apoptotic features using different lengths of incubation. Over time, the majority of cells remained in a mitotically inactive state. The percentage of apoptotic cells increased from day 2 to day 10, at the same time as the necrotic ones increased. The HS181 hESC line grew in an undifferentiated state on inactive HFF throughout the study.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Nieto A,Cabrera CM,Catalina P,Cobo F,Barnie A,Cortés JL,Barroso del Jesus A,Montes R,Concha Adoi
10.1016/j.cellbi.2006.11.006subject
Has Abstractpub_date
2007-03-01 00:00:00pages
269-78issue
3eissn
1065-6995issn
1095-8355pii
S1065-6995(06)00270-8journal_volume
31pub_type
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