Abstract:
:Following culture of human peripheral blood mononuclear cells (PBMNC) from 25 normal donors and 15 patients with common variable immunodeficiency (CVID), we were unable to identify any IL10-defective patients. Clear-cut effects of IL4 could be demonstrated in controls, while in CVID all effects are less pronounced. While in both controls and CVID baseline levels of IL6, IgG and IgM were found to be correlated, this was altered by the addition of either IL4 or Poke Weed Mitogen (PWM). We therefore conclude that the inability of PBMNC to produce IL10 is not the cause of CVID in our patients. In CVID, the regulating circuitry triggered by IL4 remains principally intact, however, some subgroups of CVID behave significantly differently.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Fritsch A,Junker U,Vogelsang H,Jager Ldoi
10.1006/cbir.1994.1030subject
Has Abstractpub_date
1994-11-01 00:00:00pages
1067-75issue
11eissn
1065-6995issn
1095-8355pii
S1065-6995(84)71030-4journal_volume
18pub_type
杂志文章abstract::Rb/E2F regulates many genes that encode proteins required for the cell cycle. Using affymetrix microarrays we previously identified genes regulated by the Rb proteins p130 and p107, many of which are involved in the cell cycle. Several genes with unknown functions were also repressed by p130 and p107, of which some ha...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.06.013
更新日期:2007-12-01 00:00:00
abstract::Hematopoietic stem and progenitor cells (HSPCs) are attractive targets in regenerative medicine, although the differences in their homeostatic maintenance between sexes along time are still under debate. We accurately monitored hematopoietic stem cells (HSCs), common lymphoid progenitors (CLPs), and common myeloid pro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10865
更新日期:2017-12-01 00:00:00
abstract::Type I diabetes is characterized by the deficiency of endocrine beta cells in the pancreatic islets of Langerhans and transplantation of islet cells can be an effective therapeutic approach. Embryonic stem cells can be differentiated into any cell type, and therefore represent an unlimited source of islet cells for th...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.12.017
更新日期:2008-04-01 00:00:00
abstract::MMP-9 participates in tumour growth, invasion, metastasis and vascularisation. Thus, inhibition of MMP-9 may be involved in the process of tumourigenesis. We have investigated the effect of RNAi-mediated MMP-9 silencing on inhibiting invasion and migration of mouse melanoma cell B16. A specific and optimised siRNA vec...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10107
更新日期:2013-08-01 00:00:00
abstract::The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the ga...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1102
更新日期:1994-07-01 00:00:00
abstract::Esophageal squamous cell carcinoma (ESCC) belongs to one of the most common malignant tumors worldwide and possesses high mortality. Long non-coding RNAs (lncRNAs) have been demonstrated to be essential biological participants in the progression of ESCC. On the basis of bio-informatics prediction, forkhead box P4 anti...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11338
更新日期:2020-07-01 00:00:00
abstract::Decline in the function of stem cells with age, such as other cells of the body, results in an imbalance between loss and renewal. Increasing age of the donor thus diminishes the effectiveness of MSCs (mesenchymal stem cells) transplantation in age-related diseases. The clinical use of stem cell therapies needs autolo...
journal_title:Cell biology international
pub_type: 杂志文章
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更新日期:2012-08-01 00:00:00
abstract::The Drosophila ovary provides an attractive model for studying the extrinsic or intrinsic factors that regulate the fate of germline stem cells (GSCs). Using this model, we identified a new role for Drosophila spaghetti (spag), encoding a homolog of human RNA polymerase II-associated protein 3 (RPAP3), in regulating t...
journal_title:Cell biology international
pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00
abstract::The microbicidal capacity of the macrophage is frequently evaded by mycobacteria, leading to tuberculosis (TB). We investigated a number of parameters affecting the rate of growth of mycobacteria in human monocyte-derived macrophages (MDM). The results show a great deal of variation in the growth of both Mycobacterium...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0679
更新日期:2001-01-01 00:00:00
abstract::Earlier, we have shown that spontaneously isolated non-pathogenic bacteria Serratia grimesii and Serratia proteamaculans invade eukaryotic cells, provided that they synthesize thermolysin-like metalloproteases ECP32/grimelysin or protealysin characterized by high specificity towards actin. To address the question of w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100314
更新日期:2011-02-01 00:00:00
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journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0837
更新日期:2002-01-01 00:00:00
abstract::In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.07.020
更新日期:2008-10-01 00:00:00
abstract::Cell therapy and regenerative medicine are potentially two of the most exciting aspects of the novel therapeutic methods currently under development. However, these treatments present a number of important biosafety issues, like the possible transmission of microorganisms to the recipients. The most common potential f...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.03.010
更新日期:2007-09-01 00:00:00
abstract::Cytoplasmic proteins with binding capability to AU-rich instability determinant sequences (ARE) of tumour necrosis factor alpha (TNFalpha) mRNA 3' untranslated region (3'UTR) were assessed in human lymphoid cells. In vitro label transfer experiments using wild type as well as mutant sequences in which the 70 nucleotid...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1999.0418
更新日期:1999-01-01 00:00:00
abstract::The dynamic state of post-translational acetylation of eukaryotic histones is maintained by histone acetyltransferases (HATs) and histone deacetylases (HDACs). HATs and HDACs have been shown to be components of various regulatory protein complexes in the cell. Their enzymatic activities, intracellular localization and...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(02)90933-9
更新日期:2002-01-01 00:00:00
abstract::Normal human bone marrow stroma cells include stem cells for both haemopoietic and osteochondrogenic lineages and express both bone morphogenetic protein (BMP) type I and type II receptors. As a member of the TGF-beta super-family, BMP-6 binds to both BMP type I and type II receptors and is involved in the development...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0662
更新日期:2001-01-01 00:00:00
abstract::Long noncoding RNAs (lncRNAs) play important roles in diverse cellular processes and carcinogenesis. Homeobox A cluster antisense RNA 2 (HOXA-AS2) is a 1,048-basepairs lncRNA located between human HOXA3 and HOXA4 genes, whose overactivation was previously found to promote the proliferation and invasion of solid tumors...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11370
更新日期:2020-08-01 00:00:00
abstract::MiR-195-5p has been shown to play an essential role in human cancer progression. Nevertheless, the biological role of miR-195-5p in osteosarcoma development remains unclear. In this study, real-time PCR was performed to examine the miR-195-5p expression in human osteosarcoma cell lines. CCK-8 assay and Transwell assay...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10723
更新日期:2017-03-01 00:00:00
abstract::This review summarizes the results of our investigations of actin unfolding-refolding and presents the notion that protein unfolding pathway, the number and the appearance order of intermediate states do not dependent on denaturing agents. To place our concept in the context of current knowledge of protein folding, we...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1016/j.cellbi.2007.01.025
更新日期:2007-04-01 00:00:00
abstract::Receptor-interacting serine/threonine kinase 4 (RIPK4) and transforming growth factor-β 1 (TGF-β1) play critical roles in the development and maintenance of the epidermis. A negative correlation between the expression patterns of RIPK4 and TGF-β signaling during epidermal homeostasis-related events and suppression of ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11282
更新日期:2020-03-01 00:00:00
abstract::The efficacies of chemotherapeutic agents are often limited by side effects and acquired drug resistance. We have investigated whether the differential expression pattern of 14-3-3σ affects cisplatin response in non-small cell lung cancer cell lines. Two pairs of parental/cisplatin resistant cell lines (A549/CRA549 an...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10006
更新日期:2013-01-01 00:00:00
abstract::Properly metabolized globin synthesis and iron uptake are indispensable for erythroid cell differentiation and maturation. Mitochondrial participation is crucial in the process of haeme synthesis for cytochromes and haemoglobin. We studied the final biosynthesis site of haemoglobin using an ultrastructural approach, w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(03)00157-4
更新日期:2003-01-01 00:00:00
abstract::A plant transformation and selection system has been developed utilizing a modified tubulin gene as a selectable marker. The vector constructs carrying a mutant alpha-tubulin gene from goosegrass conferring resistance to dinitroaniline herbicides were created for transformation of monocotyledonous and dicotyledonous p...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.11.012
更新日期:2008-05-01 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is a serious global health problem. Recently, accumulating microRNA (miRNA) has emerged as crucial players in the development and progression of carcinomas including OSCC. Our study aimed to further investigate the roles of miR-140-5p in OSCC tumorigenesis and related molecular basi...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11213
更新日期:2019-08-08 00:00:00
abstract::CD25+Treg cells (CD4+CD25+Foxp3+ regulatory T cells) play a central role in the maintenance of peripheral self-tolerance and immune homoeostasis. A previous study showed that CD25+Treg cells suppressed the differentiation and function of Th1 cells in vivo and in vitro. However, the mechanism of suppressing Th1 cell di...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20100528
更新日期:2011-07-01 00:00:00
abstract::We analyzed whether the embryonic (E12.5-E14.5) mouse retina possesses genuine neural stem cells and how they respond to defined growth factors and extracellular matrix molecules. Whereas most combinations produced no or limited cell survival and proliferation in culture, FGF-2 plus heparin and laminin stimulated prol...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.11.031
更新日期:2007-07-01 00:00:00
abstract::Bisphosphonates (BPs), especially zoledronic acid (ZOL), are clinically used to treat osteolytic bone lesions. However, serious side-effects may be also induced during the therapeutic process. To improve the BPs drugs, here, we investigated the effects of a series of ZOL derivatives with increasing number of methylene...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11546
更新日期:2021-01-06 00:00:00
abstract::vasa (vas)-related genes are members of the DEAD-box protein family and are expressed in the germ cells of many Metazoa. We cloned vasa-related genes (PpVLG, CpVLG) and other DEAD-box family related genes (PpDRH1, PpDRH2, CpDRH, AtDRHr) from the colonial parasitic rhizocephalan barnacle Polyascus polygenea, the non-co...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.09.012
更新日期:2007-02-01 00:00:00
abstract::An effect of magnesium on calcium-induced depolarisation of mitochondrial transmembrane potential (DeltaPsi(m)) was investigated. Depending on the presence of Mg(2+), addition of Ca(2+) to suspension of isolated rat heart mitochondria induced either reversible depolarisation or irreversible collapse of succinate-drive...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.08.024
更新日期:2008-01-01 00:00:00
abstract::The rates of intracellular protein degradation, of identically labelled populations of proteins, were compared in hepatocytes cultured at 37 degrees (on an adsorbed collagen layer) and in cells preserved on gelatin gels at 10 degrees C. The half-lives of the long-lived proteins were 35.4+/-8.6 h (N=4) and 692.9+/-216....
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0333
更新日期:1999-01-01 00:00:00