Abstract:
:Hematopoietic stem and progenitor cells (HSPCs) are attractive targets in regenerative medicine, although the differences in their homeostatic maintenance between sexes along time are still under debate. We accurately monitored hematopoietic stem cells (HSCs), common lymphoid progenitors (CLPs), and common myeloid progenitors (CMPs) frequencies by flow cytometry, by performing serial peripheral blood extractions from male and female B6SJL wild-type mice and found no significant differences. Only modest differences were found in the gene expression profile of Slamf1 and Gata2. Our findings suggest that both sexes could be used indistinctly to perform descriptive studies in the murine hematopoietic system, especially for flow cytometry studies in peripheral blood. This would allow diminishing the number of animals needed for the experimental procedures. In addition, the use of serial extractions in the same animals drastically decreases the number of animals needed.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Gasco S,Rando A,Zaragoza P,García-Redondo A,Calvo AC,Osta Rdoi
10.1002/cbin.10865subject
Has Abstractpub_date
2017-12-01 00:00:00pages
1399-1405issue
12eissn
1065-6995issn
1095-8355journal_volume
41pub_type
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