Abstract:
:We have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The reprogrammed iPS cells morphologically resemble human cardiomyocytes which can beat. RT-PCR and immunostaining show that cardiac markers are expressed that are comparable to the differentiation pattern of authentic human embryonic stem cells, indicating the existence of both immature and mature differentiated cardiomyocytes. 5-Azacytidine greatly enhanced the efficiency of cardiomyocyte differentiation, whereas dimethylsulfoxide had no effect. Low serum and bone morphogenetic protein-2 marginally improved differentiation efficiency. iPS cell-derived cardiomyocytes changed their beat frequency in response to cardiac drugs, which included ion channel blockers and alpha/beta adrenergic stimulators. Derived cardiomyocytes look promising as an in vitro system for potential drug screen and/or toxicity, making this system closer to practical use in the near future.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Gai H,Leung EL,Costantino PD,Aguila JR,Nguyen DM,Fink LM,Ward DC,Ma Ydoi
10.1016/j.cellbi.2009.08.008subject
Has Abstractpub_date
2009-11-01 00:00:00pages
1184-93issue
11eissn
1065-6995issn
1095-8355pii
S1065-6995(09)00196-6journal_volume
33pub_type
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