Combined application of in vivo UV-crosslinking and in vitro label transfer in the examination of AU-rich sequence binding protein-RNA interactions.

Abstract:

:A combination of in vivo UV light-induced crosslinking of nucleic acids to proteins and in vitro label transfer assay was applied to investigate specific interactions between AU-rich sequences (ARE) in the 3' UTR of lymphokine mRNAs and cytoplasmic AU-rich sequence element binding proteins (AUBP) in normal human lymphoblasts and MLA 144 gibbon lymphoid tumor cells. We demonstrate that a pool of cytoplasmic AUBP can be effectively crosslinked to RNA in vivo, suggesting a close association of these proteins with ARE sequences in the cytoplasm. We also show that the UV-crosslinked AUBP pool is markedly reduced in malignantly transformed MLA 144 cells compared with normal lymphoblasts, indicating weaker interaction between lymphokine ARE and AUBP in these tumor cells. Similar differences in AUBP-RNA associations were found between the membrane-bound polysomal subfractions of the two cell types where most of the AUBP activity was localized. We suggest that the decreased AUBP-mRNA association in MLA 144 cells might reflect a process concerned with disturbances of mRNA metabolism in the neoplastic phenotype.

journal_name

Cell Biol Int

authors

Henics T,Nagy E,Rigby WF

doi

10.1006/cbir.1995.1131

subject

Has Abstract

pub_date

1995-09-01 00:00:00

pages

791-801

issue

9

eissn

1065-6995

issn

1095-8355

pii

S1065-6995(85)71131-6

journal_volume

19

pub_type

杂志文章
  • Mesenchymal stem cell based therapy for osteo-diseases.

    abstract::Stem cell therapy in recent years has gained much attention as the modern therapeutic approach to treat diseases. Mesenchymal stem cells (MSCs) are seen as the most reliable cells applied in therapy over other stem cells because of their versatility. Bone and cartilage diseases (osteo-diseases) are the major target of...

    journal_title:Cell biology international

    pub_type: 杂志文章,评审

    doi:10.1002/cbin.10293

    authors: Ghasroldasht MM,Irfan-Maqsood M,Matin MM,Bidkhori HR,Naderi-Meshkin H,Moradi A,Bahrami AR

    更新日期:2014-10-01 00:00:00

  • Trolox-induced cardiac differentiation is mediated by the inhibition of Wnt/β-catenin signaling in human embryonic stem cells.

    abstract::Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β-catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6-Hydroxy-2,5...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11200

    authors: Choe MS,Yeo HC,Bae CM,Han HJ,Baek KM,Kim JS,Lim KS,Shin IS,Chang W,Yun SP,Lee HJ,Lee MY

    更新日期:2019-07-10 00:00:00

  • Effects of coumarin and 7OH-coumarin on bcl-2 and Bax expression in two human lung cancer cell lines in vitro.

    abstract::Coumarin and its derivative 7-hydroxycoumarin (7-OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl-2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu-1. The cells were cultured in vitro...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2005.04.003

    authors: Elinos-Báez CM,León F,Santos E

    更新日期:2005-08-01 00:00:00

  • Upregulation of HO-1 is accompanied by activation of p38MAPK and mTOR in human oesophageal squamous carcinoma cells.

    abstract::Induction of HO-1 protein can have both beneficial and detrimental effects for cells, including regulating proliferation and apoptosis of several tumours. We have investigated the regulation of HO-1, p38MAPK and mTOR in the context of proliferation, cell cycle events and apoptosis of oesophageal squamous cell carcinom...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10075

    authors: Hu JL,Xiao L,Li ZY,Wang Q,Chang Y,Jin Y

    更新日期:2013-06-01 00:00:00

  • The level and phosphorylation of Hsp70 in the rat liver cytosol after adrenalectomy and hyperthermia.

    abstract::Hepatic heat shock protein Hsp70 synthesis and in vitro phosphorylation were studied in the liver cytosol of intact, adrenalectomized and dexamethasone-administered adrenalectomized rats after 41 degrees C whole body hyperthermic stress. Hsp70 was detected by immunoblotting with N27F3-4 monoclonal antibody recognizing...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1998.0247

    authors: Cvoro A,Dundjerski J,Trajković D,Matić G

    更新日期:1999-01-01 00:00:00

  • High resolution scanning force microscopy of cardiac myocytes.

    abstract::The advent of scanning probe microscopy has introduced a powerful new method of probing the structural features of biological specimens. In this study, high resolution atomic force microscopy micrographs of single, isolated, cardiac myocytes are presented. Significantly, our images show not only the features to be exp...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0813

    authors: Davis JJ,Hill HA,Powell T

    更新日期:2001-01-01 00:00:00

  • Detection of potassium currents and regulation of multidrug resistance by potassium channels in human gastric cancer cells.

    abstract::The present study aimed to investigate the potassium currents and further explore the role of potassium channels in drug response of gastric cancer cells. By patch-clamp technique, potassium currents of human gastric cancer cell SGC7901 were recorded in the mode of voltage clamp. Both 4-aminopyridine (4-AP) and tetrae...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2007.01.008

    authors: Han Y,Shi Y,Han Z,Sun L,Fan D

    更新日期:2007-07-01 00:00:00

  • Fndc5 overexpression facilitated neural differentiation of mouse embryonic stem cells.

    abstract::Fndc5 has been recently recognized as a myokine which could be cleaved and secreted into blood stream. It is termed as irisin with an important role in thermogenesis and energy homeostasis. Increased expression of Fndc5 has been reported upon retinoic acid treatment during neural differentiation and its knockdown decr...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10427

    authors: Forouzanfar M,Rabiee F,Ghaedi K,Beheshti S,Tanhaei S,Shoaraye Nejati A,Jodeiri Farshbaf M,Baharvand H,Nasr-Esfahani MH

    更新日期:2015-05-01 00:00:00

  • The perilipin-2 (adipophilin) coat of cytosolic lipid droplets is regulated by an Arf1-dependent mechanism in HC11 mouse mammary epithelial cells.

    abstract::The cytosolic lipid droplets (cLDs) store excess intracellular lipids, and perilipin-2 is believed to protect cLDs from degradation. Here, we investigated the role of the small G-protein Arf1 and the proteasome in the fates of perilipin-2 and cLDs. In oleate-loaded cells, upon brefeldin A (BFA) treatment, perilipin-2 ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10547

    authors: Pauloin A,Adenot P,Hue-Beauvais C,Chanat E

    更新日期:2016-02-01 00:00:00

  • WITHDRAWN: Proteinase activated receptor-2 mediated IL-5, IL-6 and RANTES release from human eosinophils.

    abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2008.01.011

    authors: Wang H,Huang Y

    更新日期:2008-01-25 00:00:00

  • Murine osteoclastogenesis suppression using conditioned media produced by melanoma or activated and non-activated Jurkat-E6 cells.

    abstract::Conditioned medium (CM) (cell secretome) is a cocktail of growth factors, cytokines, and other soluble mediators secreted by cells into a culture medium. These growth factors are fundamental in many cellular processes such as cell growth, differentiation, and others and the composition of these factors is individual f...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11317

    authors: Mambelli-Lisboa NC,Frare EO,da Costa-Neves A,Prieto da Silva ÁRB,Kerkis I

    更新日期:2020-05-01 00:00:00

  • Differential sensitivity of human hepatoma cell line and primary rat hepatocyte culture to benzo(a)pyrene-induced unscheduled DNA synthesis and adduct formation.

    abstract::We studied the genotoxic potential of a carcinogen in the human hepatoma cell line, HepG2, and in primary rat hepatocyte culture. HepG2 is a well differentiated human hepatoblastoma cell line with biotransforming capacity. Rat hepatocytes were obtained by the standard two-step in situ perfusion technique. Following be...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1993.1083

    authors: Liu TY,Chao TW,Chiang SH,Chi CW

    更新日期:1993-04-01 00:00:00

  • Behaviour of dissociated hypoblast cells on the basal lamina and on extracellular fibrils in the gastrulating chicken embryo.

    abstract::The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the ga...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1994.1102

    authors: Andries L,Harrisson F,Vakaet L

    更新日期:1994-07-01 00:00:00

  • Bone marrow derived mesenchymal stem cells from aged mice have reduced wound healing, angiogenesis, proliferation and anti-apoptosis capabilities.

    abstract::Decline in the function of stem cells with age, such as other cells of the body, results in an imbalance between loss and renewal. Increasing age of the donor thus diminishes the effectiveness of MSCs (mesenchymal stem cells) transplantation in age-related diseases. The clinical use of stem cell therapies needs autolo...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110183

    authors: Choudhery MS,Khan M,Mahmood R,Mehmood A,Khan SN,Riazuddin S

    更新日期:2012-08-01 00:00:00

  • TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.

    abstract::Tripartite motif-containing 22 (TRIM22) is reported to participate in numerous cellular activities. Recent studies confirm that TRIM22 is a target gene for P53, and inhibits clonogenic growth of leukemic U-937 cells. The current study aims to discover the effect of TRIM22 in progression of human chronic myeloid leukem...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10989

    authors: Li L,Qi Y,Ma X,Xiong G,Wang L,Bao C

    更新日期:2018-09-01 00:00:00

  • Peroxynitrite activates K+-Cl- cotransport in human erythrocytes.

    abstract::Peroxynitrite was found to induce the release of K+ via the Na+/Cl- cotransport system, as do other oxidants. Since peroxynitrite is formed in vivo, its presence could contribute to a pathological dehydration of red blood cells. ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2001.0778

    authors: Grzelak A,Mazur J,Bartosz G

    更新日期:2001-01-01 00:00:00

  • Effect of estrogen on chick primordial follicle development and activation.

    abstract::Steroid hormones and their receptors play pivotal roles in avian reproduction and development. 17β-estradiol (E2 ) is an essential mediator in ovarian development. In this study, Day 3 (D3) chicks were injected with E2 (0.025, 0.25 or 2.5 mg/kg body weight) for 4 days to assess the effects of estrogen on avian follicl...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10766

    authors: Zhao D,Lv C,Liu G,Mi Y,Zhang C

    更新日期:2017-06-01 00:00:00

  • FAM107B is regulated by S100A4 and mediates the effect of S100A4 on the proliferation and migration of MGC803 gastric cancer cells.

    abstract::FAM107B expression was decreased in stomach cancer and many other kinds of cancer. The forced expression of FAM107B in HeLa cells diminished proliferation in response to growth factors, suggesting that FAM107B might play important roles in many types of cancers. But the mechanisms underlying the decreased expression o...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10816

    authors: Guo J,Bian Y,Wang Y,Chen L,Yu A,Sun X

    更新日期:2017-10-01 00:00:00

  • DNA methyltransferase inhibitor RG108 and histone deacetylase inhibitors cooperate to enhance NB4 cell differentiation and E-cadherin re-expression by chromatin remodelling.

    abstract::Epigenetic silencing of cancer-related genes by abnormal methylation and the reversal of this process by DNA methylation inhibitors represents a promising strategy in cancer therapy. As DNA methylation affects gene expression and chromatin structure, we investigated the effects of novel DNMT (DNA methyltransferase) in...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1042/CBI20110649

    authors: Savickiene J,Treigyte G,Jazdauskaite A,Borutinskaite VV,Navakauskiene R

    更新日期:2012-11-01 00:00:00

  • Neuroprotective effects of tetramethylpyrazine on hydrogen peroxide-induced apoptosis in PC12 cells.

    abstract::In the present study, we investigated the effects of tetramethylpyrazine (TMP) on hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells. The apoptosis in H2O2-induced PC12 cells was accompanied by a decrease in Bcl-2/Bax protein ratio, release of cytochrome c to cytosol and the activation of caspase-3. TMP not only...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/j.cellbi.2006.10.001

    authors: Cheng XR,Zhang L,Hu JJ,Sun L,Du GH

    更新日期:2007-05-01 00:00:00

  • Glycometabolic reprogramming associated with the initiation of human dental pulp stem cell differentiation.

    abstract::Glycometabolism, particularly mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, plays a central role in cell life activities. Glycometabolism can be reprogrammed to maintain the stemness or to induce the differentiation of stem cells, thereby regulating tissue repair and regeneration. However, research ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10568

    authors: Wang L,Cheng L,Wang H,Pan H,Yang H,Shao M,Hu T

    更新日期:2016-03-01 00:00:00

  • Spectrin-like proteins in plant nuclei.

    abstract::We analysed the presence and localization of spectrin-like proteins in nuclei of various plant tissues, using several anti-erythrocyte spectrin antibodies on isolated pea nuclei and nuclei in cells. Western blots of extracted purified pea nuclei show a cross-reactive pair of bands at 220-240 kDa, typical for human ery...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2000.0508

    authors: De Ruijter NC,Ketelaar T,Blumenthal SS,Emons AM,Schel JH

    更新日期:2000-01-01 00:00:00

  • Effect of melanins from black yeast fungi on proliferation and differentiation of cultivated human keratinocytes and fibroblasts.

    abstract::The effects of melanin preparations from black yeast fungi (BYF) on the proliferation and differentiation of normal cultivated human skin keratinocytes and embryonic pulmonary fibroblasts have been investigated. Melanin preparations in the range of 5-0.1 microg/ml were optimally active, with a more pronounced effect o...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1016/s1065-6995(02)00297-4

    authors: Blinova MI,Yudintseva NM,Kalmykova NV,Kuzminykh EV,Yurlova NA,Ovchinnikova OA,Potokin IL

    更新日期:2003-01-01 00:00:00

  • Drosophila splicing factor SF2 knock-down mutant shows altered cell-cycle in vivo.

    abstract::Pioneering single gene study documents pre-mRNA processing proteins participation in the cell-cycle regulation of multi-cellular animals (Andersen and Tapon, 2008, J Biol Chem 283: 31256-67). Whole-genome RNAi screen in Drosophila tissue-culture cell lines demonstrates that 17 genes involved in RNA-processing are requ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10019

    authors: Dubatolova TD,Volkova EI,Omelyanchuk LV

    更新日期:2013-02-01 00:00:00

  • Uptake of cadmium ions in white-rot fungus Trametes versicolor: effect of cd (II) ions on the activity of laccase.

    abstract::The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.2002.0887

    authors: Jarosz-Wilkolazka A,Malarczyk E,Pirszel J,Skowroński T,Leonowicz A

    更新日期:2002-01-01 00:00:00

  • Serpin A1 and the modulation of type I collagen turnover: Effect of the C-terminal peptide 409-418 (SA1-III) in human dermal fibroblasts.

    abstract::The pharmacological modulation of collagen turnover is a strategy potentially useful in different skin conditions. The serine protease inhibitor Serpin A1 and portions of its C-terminal region have been investigated as collagen modulators. To clarify the mechanisms by which the C-terminal 409-418 peptide SA1-III incre...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.11018

    authors: Cipriani C,Pascarella S,Errante F,Menicacci B,Magnelli L,Mocali A,Rovero P,Giovannelli L

    更新日期:2018-09-01 00:00:00

  • Cytokeratin expression in human respiratory epithelium of nasal polyps and turbinates.

    abstract::The cytokertatins in respiratory epithelial cells (REC) of human nasal polyps and turbinates were analyzed by immunohistochemistry. Cytokeratin 19 (CK19) was present in all REC, CK5 and 14 were expressed primarily in basal cells, and CK7, 8, and 18 were found in suprabasal cells. Differences in cytoplasmic locations w...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1995.1072

    authors: Hicks W Jr,Ward R,Edelstein D,Hall L 3rd,Albino A,Hard R,Asch B

    更新日期:1995-04-01 00:00:00

  • Studies on nitric oxide free radicals generated from polymorphonuclear leukocytes (PMN) stimulated by phorbol myristate acetate (PMA).

    abstract::The interaction of NO and O2- free radicals generated from PMA (phorbol myristate acetate)-stimulated PMN (polymorphonuclear leukocytes) was studied by a nitroxide spin trap, DMPO (5,5-dimethyl-1-pyrroline-1-oxide). It was found that addition of L-arginine to the system would significantly decrease the trapped O2- by ...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1006/cbir.1996.0039

    authors: Zhao BL,Wang JC,Hou JW,Xin WJ

    更新日期:1996-05-01 00:00:00

  • Adrenaline inhibits osteogenesis via repressing miR-21 expression.

    abstract::Sympathetic signaling is involved in bone homeostasis; however, the cellular and molecular mechanisms remain unknown. In this study, we found that the psychological stress mediator adrenaline inhibited osteogenic differentiation of human bone marrow-derived stem cells (hMSC) by reducing microRNA-21 (miR-21) expression...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10685

    authors: Chen D,Wang Z

    更新日期:2017-01-01 00:00:00

  • Retinoic acid can induce mouse embryonic stem cell R1/E to differentiate toward female germ cells while oleanolic acid can induce R1/E to differentiate toward both types of germ cells.

    abstract::Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC-R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC-R1/E and EBs were allowed to attach to the bottoms of normal cell-culturing plate and grow. Then, different...

    journal_title:Cell biology international

    pub_type: 杂志文章

    doi:10.1002/cbin.10380

    authors: Wan Q,Lu H,Wu LT,Liu X,Xiang JB

    更新日期:2014-12-01 00:00:00