Abstract:
:A combination of in vivo UV light-induced crosslinking of nucleic acids to proteins and in vitro label transfer assay was applied to investigate specific interactions between AU-rich sequences (ARE) in the 3' UTR of lymphokine mRNAs and cytoplasmic AU-rich sequence element binding proteins (AUBP) in normal human lymphoblasts and MLA 144 gibbon lymphoid tumor cells. We demonstrate that a pool of cytoplasmic AUBP can be effectively crosslinked to RNA in vivo, suggesting a close association of these proteins with ARE sequences in the cytoplasm. We also show that the UV-crosslinked AUBP pool is markedly reduced in malignantly transformed MLA 144 cells compared with normal lymphoblasts, indicating weaker interaction between lymphokine ARE and AUBP in these tumor cells. Similar differences in AUBP-RNA associations were found between the membrane-bound polysomal subfractions of the two cell types where most of the AUBP activity was localized. We suggest that the decreased AUBP-mRNA association in MLA 144 cells might reflect a process concerned with disturbances of mRNA metabolism in the neoplastic phenotype.
journal_name
Cell Biol Intjournal_title
Cell biology internationalauthors
Henics T,Nagy E,Rigby WFdoi
10.1006/cbir.1995.1131subject
Has Abstractpub_date
1995-09-01 00:00:00pages
791-801issue
9eissn
1065-6995issn
1095-8355pii
S1065-6995(85)71131-6journal_volume
19pub_type
杂志文章abstract::Stem cell therapy in recent years has gained much attention as the modern therapeutic approach to treat diseases. Mesenchymal stem cells (MSCs) are seen as the most reliable cells applied in therapy over other stem cells because of their versatility. Bone and cartilage diseases (osteo-diseases) are the major target of...
journal_title:Cell biology international
pub_type: 杂志文章,评审
doi:10.1002/cbin.10293
更新日期:2014-10-01 00:00:00
abstract::Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β-catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6-Hydroxy-2,5...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11200
更新日期:2019-07-10 00:00:00
abstract::Coumarin and its derivative 7-hydroxycoumarin (7-OHC) have antitumor and antimetastatic properties. The purpose of this study was to investigate the possible effects of these compounds on expression of the bcl-2 and Bax oncoproteins in two human lung cancer cell lines, A427 and Calu-1. The cells were cultured in vitro...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2005.04.003
更新日期:2005-08-01 00:00:00
abstract::Induction of HO-1 protein can have both beneficial and detrimental effects for cells, including regulating proliferation and apoptosis of several tumours. We have investigated the regulation of HO-1, p38MAPK and mTOR in the context of proliferation, cell cycle events and apoptosis of oesophageal squamous cell carcinom...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10075
更新日期:2013-06-01 00:00:00
abstract::Hepatic heat shock protein Hsp70 synthesis and in vitro phosphorylation were studied in the liver cytosol of intact, adrenalectomized and dexamethasone-administered adrenalectomized rats after 41 degrees C whole body hyperthermic stress. Hsp70 was detected by immunoblotting with N27F3-4 monoclonal antibody recognizing...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1998.0247
更新日期:1999-01-01 00:00:00
abstract::The advent of scanning probe microscopy has introduced a powerful new method of probing the structural features of biological specimens. In this study, high resolution atomic force microscopy micrographs of single, isolated, cardiac myocytes are presented. Significantly, our images show not only the features to be exp...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0813
更新日期:2001-01-01 00:00:00
abstract::The present study aimed to investigate the potassium currents and further explore the role of potassium channels in drug response of gastric cancer cells. By patch-clamp technique, potassium currents of human gastric cancer cell SGC7901 were recorded in the mode of voltage clamp. Both 4-aminopyridine (4-AP) and tetrae...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2007.01.008
更新日期:2007-07-01 00:00:00
abstract::Fndc5 has been recently recognized as a myokine which could be cleaved and secreted into blood stream. It is termed as irisin with an important role in thermogenesis and energy homeostasis. Increased expression of Fndc5 has been reported upon retinoic acid treatment during neural differentiation and its knockdown decr...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10427
更新日期:2015-05-01 00:00:00
abstract::The cytosolic lipid droplets (cLDs) store excess intracellular lipids, and perilipin-2 is believed to protect cLDs from degradation. Here, we investigated the role of the small G-protein Arf1 and the proteasome in the fates of perilipin-2 and cLDs. In oleate-loaded cells, upon brefeldin A (BFA) treatment, perilipin-2 ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10547
更新日期:2016-02-01 00:00:00
abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2008.01.011
更新日期:2008-01-25 00:00:00
abstract::Conditioned medium (CM) (cell secretome) is a cocktail of growth factors, cytokines, and other soluble mediators secreted by cells into a culture medium. These growth factors are fundamental in many cellular processes such as cell growth, differentiation, and others and the composition of these factors is individual f...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11317
更新日期:2020-05-01 00:00:00
abstract::We studied the genotoxic potential of a carcinogen in the human hepatoma cell line, HepG2, and in primary rat hepatocyte culture. HepG2 is a well differentiated human hepatoblastoma cell line with biotransforming capacity. Rat hepatocytes were obtained by the standard two-step in situ perfusion technique. Following be...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1993.1083
更新日期:1993-04-01 00:00:00
abstract::The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the ga...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1994.1102
更新日期:1994-07-01 00:00:00
abstract::Decline in the function of stem cells with age, such as other cells of the body, results in an imbalance between loss and renewal. Increasing age of the donor thus diminishes the effectiveness of MSCs (mesenchymal stem cells) transplantation in age-related diseases. The clinical use of stem cell therapies needs autolo...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110183
更新日期:2012-08-01 00:00:00
abstract::Tripartite motif-containing 22 (TRIM22) is reported to participate in numerous cellular activities. Recent studies confirm that TRIM22 is a target gene for P53, and inhibits clonogenic growth of leukemic U-937 cells. The current study aims to discover the effect of TRIM22 in progression of human chronic myeloid leukem...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10989
更新日期:2018-09-01 00:00:00
abstract::Peroxynitrite was found to induce the release of K+ via the Na+/Cl- cotransport system, as do other oxidants. Since peroxynitrite is formed in vivo, its presence could contribute to a pathological dehydration of red blood cells. ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2001.0778
更新日期:2001-01-01 00:00:00
abstract::Steroid hormones and their receptors play pivotal roles in avian reproduction and development. 17β-estradiol (E2 ) is an essential mediator in ovarian development. In this study, Day 3 (D3) chicks were injected with E2 (0.025, 0.25 or 2.5 mg/kg body weight) for 4 days to assess the effects of estrogen on avian follicl...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10766
更新日期:2017-06-01 00:00:00
abstract::FAM107B expression was decreased in stomach cancer and many other kinds of cancer. The forced expression of FAM107B in HeLa cells diminished proliferation in response to growth factors, suggesting that FAM107B might play important roles in many types of cancers. But the mechanisms underlying the decreased expression o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10816
更新日期:2017-10-01 00:00:00
abstract::Epigenetic silencing of cancer-related genes by abnormal methylation and the reversal of this process by DNA methylation inhibitors represents a promising strategy in cancer therapy. As DNA methylation affects gene expression and chromatin structure, we investigated the effects of novel DNMT (DNA methyltransferase) in...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1042/CBI20110649
更新日期:2012-11-01 00:00:00
abstract::In the present study, we investigated the effects of tetramethylpyrazine (TMP) on hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells. The apoptosis in H2O2-induced PC12 cells was accompanied by a decrease in Bcl-2/Bax protein ratio, release of cytochrome c to cytosol and the activation of caspase-3. TMP not only...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/j.cellbi.2006.10.001
更新日期:2007-05-01 00:00:00
abstract::Glycometabolism, particularly mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis, plays a central role in cell life activities. Glycometabolism can be reprogrammed to maintain the stemness or to induce the differentiation of stem cells, thereby regulating tissue repair and regeneration. However, research ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10568
更新日期:2016-03-01 00:00:00
abstract::We analysed the presence and localization of spectrin-like proteins in nuclei of various plant tissues, using several anti-erythrocyte spectrin antibodies on isolated pea nuclei and nuclei in cells. Western blots of extracted purified pea nuclei show a cross-reactive pair of bands at 220-240 kDa, typical for human ery...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2000.0508
更新日期:2000-01-01 00:00:00
abstract::The effects of melanin preparations from black yeast fungi (BYF) on the proliferation and differentiation of normal cultivated human skin keratinocytes and embryonic pulmonary fibroblasts have been investigated. Melanin preparations in the range of 5-0.1 microg/ml were optimally active, with a more pronounced effect o...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1016/s1065-6995(02)00297-4
更新日期:2003-01-01 00:00:00
abstract::Pioneering single gene study documents pre-mRNA processing proteins participation in the cell-cycle regulation of multi-cellular animals (Andersen and Tapon, 2008, J Biol Chem 283: 31256-67). Whole-genome RNAi screen in Drosophila tissue-culture cell lines demonstrates that 17 genes involved in RNA-processing are requ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10019
更新日期:2013-02-01 00:00:00
abstract::The effects of cadmium Cd (II) ions on the physiology and biological activity of Trametes versicolor, a strain belonging to white-rotting Basidiomycetes, were examined. Cd (II) ions were added to 10-day-old cultures grown on a liquid medium, or at the time of inoculation. Our experiments showed that T. versicolor is a...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.2002.0887
更新日期:2002-01-01 00:00:00
abstract::The pharmacological modulation of collagen turnover is a strategy potentially useful in different skin conditions. The serine protease inhibitor Serpin A1 and portions of its C-terminal region have been investigated as collagen modulators. To clarify the mechanisms by which the C-terminal 409-418 peptide SA1-III incre...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.11018
更新日期:2018-09-01 00:00:00
abstract::The cytokertatins in respiratory epithelial cells (REC) of human nasal polyps and turbinates were analyzed by immunohistochemistry. Cytokeratin 19 (CK19) was present in all REC, CK5 and 14 were expressed primarily in basal cells, and CK7, 8, and 18 were found in suprabasal cells. Differences in cytoplasmic locations w...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1995.1072
更新日期:1995-04-01 00:00:00
abstract::The interaction of NO and O2- free radicals generated from PMA (phorbol myristate acetate)-stimulated PMN (polymorphonuclear leukocytes) was studied by a nitroxide spin trap, DMPO (5,5-dimethyl-1-pyrroline-1-oxide). It was found that addition of L-arginine to the system would significantly decrease the trapped O2- by ...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1006/cbir.1996.0039
更新日期:1996-05-01 00:00:00
abstract::Sympathetic signaling is involved in bone homeostasis; however, the cellular and molecular mechanisms remain unknown. In this study, we found that the psychological stress mediator adrenaline inhibited osteogenic differentiation of human bone marrow-derived stem cells (hMSC) by reducing microRNA-21 (miR-21) expression...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10685
更新日期:2017-01-01 00:00:00
abstract::Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC-R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC-R1/E and EBs were allowed to attach to the bottoms of normal cell-culturing plate and grow. Then, different...
journal_title:Cell biology international
pub_type: 杂志文章
doi:10.1002/cbin.10380
更新日期:2014-12-01 00:00:00