TATA sequence requirements for the initiation of transcription for an RNA polymerase II in vitro transcription system from Nicotiana tabacum.

abstract：:To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-NPTII reporter gene in a T-DNA construct. A number of transgenic N. sy...

journal_title：Plant molecular biology

authors： Kunz C,Narangajavana J,Jakowitsch J,Park YD,Delon TR,Kovarik A,Koukalová B,van der Winden J,Moscone E,Aufsatz W,Mette MF,Matzke M,Matzke AJ

更新日期：2003-05-01 00:00:00

abstract：:Chromosomal non-histone high-mobility-group (HMG) proteins represent essential components of eukaryotic chromatin and have also been isolated from a variety of plants. In maize, studies on structure and function of the two larger of the four major HMG proteins have recently been performed and are now extended by analy...

journal_title：Plant molecular biology

authors： Grasser KD,Hetz W,Feix G

更新日期：1994-06-01 00:00:00

abstract：:Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...

journal_title：Plant molecular biology

authors： Benkovics AH,Nyikó T,Mérai Z,Silhavy D,Bisztray GD

更新日期：2011-02-01 00:00:00

abstract：KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...

journal_title：Plant molecular biology

authors： Rose AB,Carter A,Korf I,Kojima N

更新日期：2016-10-01 00:00:00

abstract：:Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...

journal_title：Plant molecular biology

authors： García-Ortiz MV,Ariza RR,Roldán-Arjona T

更新日期：2001-12-01 00:00:00

abstract：:Cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. The gene products participate either directly or indirectly towards increasing cold tolerance. Evidence of proteins having a direct effect on cold tolerance is emerging but limited. With isolated protoplasts from warm...

journal_title：Plant molecular biology

authors： Newton SS,Duman JG

更新日期：2000-11-01 00:00:00

abstract：:Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...

journal_title：Plant molecular biology

authors： van den Elzen P,Lee KY,Townsend J,Bedbrook J

更新日期：1985-05-01 00:00:00

abstract：:A seedling lethal mutant of Nicotiana plumbaginifolia (sdl-1) was isolated by transposon tagging using a maize Dissociation (Ds) element. The insertion mutation was produced by direct co-transformation of protoplasts with two plasmids: one containing Ds and a second with an Ac transposase gene. sdl-1 seedlings exhibit...

journal_title：Plant molecular biology

authors： Majira A,Domin M,Grandjean O,Gofron K,Houba-Hérin N

更新日期：2002-10-01 00:00:00

abstract：:Secretion, endocytosis and transport to the lytic compartment are fundamental, highly coordinated features of the eukaryotic cell. These intracellular transport processes are facilitated by vesicles, many of which are small (100 nm or less in diameter) and 'coated' on their cytoplasmic surface. Research into the struc...

journal_title：Plant molecular biology

authors： Robinson DG,Hinz G,Holstein SE

更新日期：1998-09-01 00:00:00

abstract：:The constitutive cytosolic expression of a yeast ( Saccharomyces cerevisiae ) invertase within potato ( Solanum tuberosum ) tubers has previously been documented to produce a dramatic metabolic phenotype in which glycolysis, respiration and amino acid synthesis are markedly enhanced at the cost of starch synthesis. Th...

journal_title：Plant molecular biology

authors： Junker BH,Wuttke R,Tiessen A,Geigenberger P,Sonnewald U,Willmitzer L,Fernie AR

更新日期：2004-09-01 00:00:00

abstract：:Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...

journal_title：Plant molecular biology

authors： Yuan F,Lyu MJ,Leng BY,Zhu XG,Wang BS

更新日期：2016-06-01 00:00:00

abstract：:A three-dimensional model of curculin, a sweet-tasting and taste-modifying protein from the fruits of Curculigo latifolia, was built from the X-ray coordinates of GNA, a mannose-binding lectin from snowdrop (Galanthus nivalis). The three mannose-binding sites present in GNA were found in curculin but are devoid of man...

journal_title：Plant molecular biology

authors： Barre A,Van Damme EJ,Peumans WJ,Rougé P

更新日期：1997-03-01 00:00:00

abstract：:We report the sequences of full-length cDNAs for the nuclear genes encoding the chloroplastic and cytosolic fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) from spinach. A comparison of the deduced amino-acid sequences with one another and with published cytosolic aldolase sequences of other plants revealed that the ...

journal_title：Plant molecular biology

authors： Pelzer-Reith B,Penger A,Schnarrenberger C

更新日期：1993-01-01 00:00:00

abstract：KEY MESSAGE:The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, howeve...

journal_title：Plant molecular biology

authors： Gong P,Luo Y,Huang F,Chen Y,Zhao C,Wu X,Li K,Yang X,Cheng F,Xiang X,Wu C,Pan G

更新日期：2019-05-01 00:00:00

abstract：:We have constructed a chimaeric gene consisting of the promoter of the soybean heat shock (hs) gene Gmhsp17, 6-L, the coding region of a hygromycin phosphotransferase (hpt) gene, and the termination sequence of the nopaline synthase (nos) gene. This gene fusion was introduced into tobacco by Agrobacterium-mediated gen...

journal_title：Plant molecular biology

authors： Severin K,Schöffl F

更新日期：1990-12-01 00:00:00

abstract：:In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...

journal_title：Plant molecular biology

authors： Showalter AM,Butt AD,Kim S

更新日期：1992-05-01 00:00:00

abstract：:In bacterial and animal coenzyme A (CoA) biosynthesis, pantothenate kinase (PANK) activity is critical in regulating intracellular CoA levels. Less is known about the role of PANK in plants, although a single plant isozyme from Arabidopsis, AtPANK1, was previously cloned and analyzed in vitro. We report here the chara...

journal_title：Plant molecular biology

authors： Tilton GB,Wedemeyer WJ,Browse J,Ohlrogge J

更新日期：2006-07-01 00:00:00

abstract：:Two S-adenosylmethionine synthetase (SAMS) cDNAs, PcSAMS1 and PcSAMS2, have been identified in Pinus contorta. We found that the two genes are differentially expressed during root development. Thus, PcSAMS1 is preferentially expressed in roots and exhibits a specific expression pattern in the meristem at the onset of ...

journal_title：Plant molecular biology

authors： Lindroth AM,Saarikoski P,Flygh G,Clapham D,Grönroos R,Thelander M,Ronne H,von Arnold S

更新日期：2001-06-01 00:00:00

abstract：:Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this development...

journal_title：Plant molecular biology

authors： Hosp J,Tashpulatov A,Roessner U,Barsova E,Katholnigg H,Steinborn R,Melikant B,Lukyanov S,Heberle-Bors E,Touraev A

更新日期：2007-01-01 00:00:00

abstract：:A cDNA library from ethephon-treated cucumber cotyledons (Cucumis sativus L. cv. Poinsett 76) was constructed. Two cDNA clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kDa cationic peroxidase that had been previously shown to be induced by...

journal_title：Plant molecular biology

authors： Morgens PH,Callahan AM,Dunn LJ,Abeles FB

更新日期：1990-05-01 00:00:00

abstract：:Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high...

journal_title：Plant molecular biology

authors： Meng Q,Du J,Li J,Lü X,Zeng X,Yuan M,Mao T

更新日期：2010-12-01 00:00:00

abstract：:The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...

journal_title：Plant molecular biology

authors： Rubinstein B

更新日期：2000-10-01 00:00:00

abstract：:Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...

journal_title：Plant molecular biology

authors： Piechulla B,Merforth N,Rudolph B

更新日期：1998-11-01 00:00:00

abstract：:Soil contamination by hexavalent chromium [Cr(VI) or chromate] due to anthropogenic activities has become an increasingly important environmental problem. To date few studies have been performed to elucidate the signaling networks involved on adaptive responses to (CrVI) toxicity in plants. In this work, we report tha...

journal_title：Plant molecular biology

authors： Martínez-Trujillo M,Méndez-Bravo A,Ortiz-Castro R,Hernández-Madrigal F,Ibarra-Laclette E,Ruiz-Herrera LF,Long TA,Cervantes C,Herrera-Estrella L,López-Bucio J

更新日期：2014-09-01 00:00:00

abstract：:We describe a method for identifying signal transducing proteins from other organisms by their ability to turn on a signalling pathway when they are expressed at high level in yeast. The method was tested on a cDNA library from Arabidopsis thaliana, which was screened for clones that can activate glucose repression in...

journal_title：Plant molecular biology

authors： Thelander M,Fredriksson D,Schouten J,Hoge JH,Ronne H

更新日期：2002-05-01 00:00:00

abstract：:Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the spli...

journal_title：Plant molecular biology

authors： Takaiwa F,Oono K,Wing D,Kato A

更新日期：1991-10-01 00:00:00

abstract：:The characterization of a non-photosynthetic isoform of NADP-malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform ...

journal_title：Plant molecular biology

authors： Maurino VG,Saigo M,Andreo CS,Drincovich MF

更新日期：2001-03-01 00:00:00

abstract：:Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleo...

journal_title：Plant molecular biology

authors： Chye ML,Cheung KY

更新日期：1995-10-01 00:00:00

abstract：:In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...

journal_title：Plant molecular biology

authors： Holme IB,Wendt T,Gil-Humanes J,Deleuran LC,Starker CG,Voytas DF,Brinch-Pedersen H

更新日期：2017-09-01 00:00:00

abstract：:The gene encoding nitrite reductase (nir) from the cyanobacterium Synechococcus sp. PCC 7942 has been identified and sequenced. This gene comprises 1536 nucleotides and would encode a polypeptide of 56,506 Da that shows similarity to nitrite reductase from higher plants and to the sulfite reductase hemoprotein from en...

journal_title：Plant molecular biology

authors： Luque I,Flores E,Herrero A

更新日期：1993-03-01 00:00:00