Abstract:
:Cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. The gene products participate either directly or indirectly towards increasing cold tolerance. Evidence of proteins having a direct effect on cold tolerance is emerging but limited. With isolated protoplasts from warm-grown kale (Brassica oleracea) as a model system, we tested protein fractions from winter bittersweet nightshade, Solanum dulcamara, stems for the presence of proteins that have a cryoprotective effect. Purification of one such fraction resulted in isolation of a 25 kDa protein. N-terminal Edman degradation amino acid sequence analysis showed that it has high homology to osmotin and osmotin-like proteins. When added to warm-grown protoplasts, it increased the cryosurvival of frozen-thawed protoplasts by 24% over untreated or BSA-treated controls at -8 degrees C. A cDNA library which was made in November from stems and leaves of S. dulcamara was successfully screened for the corresponding cDNA clone. The deduced amino acid sequence indicated that the protein consists of 206 amino acid residues including a N-terminal signal sequence and a putative C-terminal propeptide. The mature protein, without the N-terminal signal sequence, was expressed in Escherichia coli. The partially purified protein in the supernatant fraction of the culture medium had cryoprotective activity.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Newton SS,Duman JGdoi
10.1023/a:1026599028063subject
Has Abstractpub_date
2000-11-01 00:00:00pages
581-9issue
5eissn
0167-4412issn
1573-5028journal_volume
44pub_type
杂志文章abstract::RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/B:PLAN.0000028797.63168.a7
更新日期:2004-01-01 00:00:00
abstract::Two cDNA clones encoding fucoxanthin chlorophyll a/c-binding proteins (FCP) in the diatom Odontella sinensis have been cloned and sequenced. The derived amino acid sequences of both clones are identical, comparison of the corresponding nucleic acids reveals differences only in the third codon position, suggesting a re...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020236
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00044158
更新日期:1989-06-01 00:00:00
abstract::We describe the molecular and functional characterization of three closely related S-adenosyl-L-methionine synthetase (SAMS) isoenzymes from Catharanthus roseus (Madagascar periwinkle). The genes are differentially expressed in cell cultures during growth of the culture and after application of various stresses (elici...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005711720930
更新日期:1997-01-01 00:00:00
abstract::To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-NPTII reporter gene in a T-DNA construct. A number of transgenic N. sy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1023937006311
更新日期:2003-05-01 00:00:00
abstract:KEY MESSAGE:To explore the function of Dof transcription factors during kernel development in maize, we first identified Dof genes in the maize genome. We found that ZmDof3 was exclusively expressed in the endosperm of maize kernel and had the features of a Dof transcription factor. Suppression of ZmDof3 resulted in a ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0543-y
更新日期:2017-01-01 00:00:00
abstract::A certain nucleotide sequence in the promoter region of Vicia faba rRNA genes that specifically binds to a nuclear protein fraction has been identified by using a gel retardation assay and DNase I footprinting technique. The binding site of this protein fraction is located about 60 bp upstream from the initiation site...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027164
更新日期:1992-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9204-5
更新日期:2007-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0319-9
更新日期:2015-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006133311402
更新日期:1999-04-01 00:00:00
abstract::Two wheat histone H2B genes (TH123 and TH153) were isolated. Nucleotide sequence analysis revealed that some characteristic sequence motifs were conserved in both the 5'- and 3'-flanking regions. A canonical TATA box and several CCAAT sequences were present in the presumed promoter regions. Motifs similar or identical...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042047
更新日期:1995-04-01 00:00:00
abstract::Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023013
更新日期:1981-03-01 00:00:00
abstract::Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020881
更新日期:1995-03-01 00:00:00
abstract::A highly purified RNA polymerase preparation from pea chloroplasts has been shown to specifically transcribe the 16S rRNA gene in vitro using the recombinant pCB2-8 DNA as a template. The RNA polymerase has been found to show maximum activity and specificity with pea supercoiled rDNA as a template. At low concentratio...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027135
更新日期:1986-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005924502336
更新日期:1998-02-01 00:00:00
abstract:KEY MESSAGE:A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco. Pigeonpea (Cajanus cajan L. Millsp.) is the only legume known to have commercial hybrid seed technology based on cytop...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0728-7
更新日期:2018-05-01 00:00:00
abstract::Despite recent progress auxin signal transduction remains largely scetchy and enigmatic. A good body of evidence supports the notion that the ABP1 could be a functional receptor or part of a receptor, respectively, but this is not generally accepted. Evidence for other functional receptors is lacking, as is any clearc...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-06-01 00:00:00
abstract::The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014183
更新日期:1987-01-01 00:00:00
abstract::The folding and assembly of proteins in cells often requires the assistance of molecular chaperones such as the Hsp70 and Hsp60 heat shock proteins. Hsp70 chaperones cooperate with DnaJ and GrpE homologues to ensure a productive folding cycle. In this study we describe the gene for the first chloroplast localized DnaJ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005784115363
更新日期:1997-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040726
更新日期:1996-08-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/b:plan.0000006936.50532.3a
更新日期:2003-10-01 00:00:00
abstract::The actin gene superfamily of Petunia hybrida cv. Mitchell contains greater than 100 gene members which have been divided into several highly divergent subfamilies [1]. Five subfamily-specific probes have been used to compare the actin genes among the Mitchell, Violet 23 (V23) and Red 51 (R51) cultivars of P. hybrida....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017466
更新日期:1988-09-01 00:00:00
abstract::Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015227
更新日期:1986-07-01 00:00:00
abstract::Soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. China has the largest collection of cultivated soybeans (Glycine max) and wild soybeans (Glycine soja) all over the world. The platform of soybean core, mini core and integrated applied c...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0076-6
更新日期:2013-09-01 00:00:00
abstract::The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1012457113700
更新日期:2001-11-01 00:00:00
abstract::Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019126
更新日期:1995-10-01 00:00:00
abstract:KEY MESSAGE:AcGST1, an anthocyanin-related GST, may functions as a carrier to transport anthocyanins from ER to tonoplast in kiwifruit. It was positively regulated by AcMYBF110 through directly binding to its promoter. Anthocyanins are synthesized in the cytoplasmic surface of the endoplasmic reticulum but accumulate p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00870-6
更新日期:2019-07-01 00:00:00
abstract:KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in st...
journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2019-11-01 00:00:00
abstract:KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00945-4
更新日期:2020-02-01 00:00:00
abstract::We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene cons...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014935
更新日期:1993-05-01 00:00:00