Abstract：
:We describe the molecular and functional characterization of three closely related S-adenosyl-L-methionine synthetase (SAMS) isoenzymes from Catharanthus roseus (Madagascar periwinkle). The genes are differentially expressed in cell cultures during growth of the culture and after application of various stresses (elicitor, nutritional down-shift, increased NaCl). Seedlings revealed organ-specific expression and differential gene regulation after salt stress. A relationship analysis indicated that plant SAMS group in two main clusters distinguished by characteristic amino acid exchanges at specific positions, and this suggested differences in the enzyme properties or the regulation. SAMS1 and SAMS2 are of type I and SAMS3 is of type II. The properties of the isoenzymes were compared after heterologous expression of the individual enzymes, but no significant differences were detected in a) optima for temperature (37 to 45 degrees C) or pH (7 to 8.3); b) dependence on cations (divalent: Mg2+, Mn2+, Co2+; monovalent: K+, NH4+, Na+); c) K(m)s for ATP and L-methionine; d) inhibition by reaction products (S-adenosyl-L-methionine, PPi, Pi), by the reaction intermediate tripolyphosphate, and by the substrate analogues ethionine and cycloleucine; e) response to metabolites from the methyl cycle (L-homocysteine) or from related pathways (L-ornithine, putrescine, spermidine, spermine); f) native protein size (gel permeation chromatography). The results represent the first characterization of plant SAMS isoenzyme properties with individually expressed proteins. The possibility is discussed that the isoenzyme differences reflect specificities in the association with enzymes that use S-adenosyl-L-methionine.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Schröder G,Eichel J,Breinig S,Schröder Jdoi
10.1023/a:1005711720930subject
Has Abstractpub_date
1997-01-01 00:00:00pages
211-22issue
2eissn
0167-4412issn
1573-5028journal_volume
33pub_type
杂志文章abstract：:Strigolactones have recently been identified as the long sought-after signal required to inhibit shoot branching (Gomez-Roldan et al. 2008; Umehara et al. 2008; reviewed in Dun et al. 2009). Here we briefly describe the evidence for strigolactone inhibition of shoot branching and, more extensively, the broader context...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-010-9599-2
更新日期：2010-05-01 00:00:00
abstract：:Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic c...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005968901298
更新日期：1998-05-01 00:00:00
abstract：:cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028878
更新日期：1994-08-01 00:00:00
abstract：KEY MESSAGE:A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco. Pigeonpea (Cajanus cajan L. Millsp.) is the only legume known to have commercial hybrid seed technology based on cytop...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0728-7
更新日期：2018-05-01 00:00:00
abstract：:The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020530
更新日期：1987-11-01 00:00:00
abstract：:We have used the bar gene in combination with the herbicide Basta to select transformed rice (Oryza sativa L. cv. Radon) protoplasts for the production of herbicide-resistant rice plants. Protoplasts, obtained from regenerable suspension cultures established from immature embryo callus, were transformed using PEG-medi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027118
更新日期：1993-03-01 00:00:00
abstract：:Neutral/Alkaline invertases are unique to plant and photosynthetic bacteria. The function of Neutral/Alkaline invertases in plant development is not clear so far. In this study, we isolated an Arabidopsis (Col-0) mutant insensitive to osmotic stress-induced inhibition on lateral root growth. Map-based cloning reveals ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9177-4
更新日期：2007-07-01 00:00:00
abstract：KEY MESSAGE:We identified and cloned the two precursors of miR158 and its target gene in Brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility, which was caused by the degradation of pollen...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0563-7
更新日期：2017-02-01 00:00:00
abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040577
更新日期：1994-01-01 00:00:00
abstract：:In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016071
更新日期：1991-06-01 00:00:00
abstract：:Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014447
更新日期：1994-03-01 00:00:00
abstract：:We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005916821224
更新日期：1998-03-01 00:00:00
abstract：:Leaf senescence is one of the key stages of plant leaf development. It is a highly complex but ordered process involving expression of large scale senescence associated genes, and its molecular mechanisms still remain unclear. By using suppression subtractive hybridization, 815 ESTs that are up-regulated at the onset ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9300-1
更新日期：2008-05-01 00:00:00
abstract：:A 47 kDa glycoprotein, termed EP4, was purified from carrot cell suspension culture medium. An antiserum raised against EP4 also recognized a protein of 45 kDa that was ionically bound to the cell wall. EP4 was detected in culture media from both embryogenic and non-embryogenic cell lines and was found to be secreted ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037018
更新日期：1995-03-01 00:00:00
abstract：:The nucleotide sequence for the Photosystem II gene psbC has been determined for the cyanobacterium Synechocystis 6803. The gene overlaps the last 50 bases of the psbD gene, and both genes are transcribed in the same direction, but read in different frames. This arrangement is identical to that found in all chloroplas...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00029879
更新日期：1988-07-01 00:00:00
abstract：:More than 600 potentially nodule-specific clones have been detected by differential hybridization of a broadbean cDNA library constructed from root nodule poly(A)+ RNA. These isolated cDNAs belong to at least 28 different clone groups containing cross-hybridizing sequences. The number of clones within a clone group va...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028969
更新日期：1993-09-01 00:00:00
abstract：:The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-013-0038-z
更新日期：2013-08-01 00:00:00
abstract：:A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tob...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020982
更新日期：1995-11-01 00:00:00
abstract：:We studied the early events of de novo formation of adventitious shoot meristems in stem segments of Brassica oleracea. A regeneration system was used that is efficient, rapid, highly responsive to cytokinins and does not involve callus formation, thus allowing studies on a direct developmental switch of cells in the ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010686931889
更新日期：2001-07-01 00:00:00
abstract：:The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005867215258
更新日期：1997-05-01 00:00:00
abstract：:Dehiscence of oilseed rape pods, commonly known as pod shatter, is a process of agronomic importance that results in seed loss causing yield reductions and carry-over of the crop into the following growing season. In an effort to understand the mechanisms underlying this developmental event, the changes in gene expres...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040589
更新日期：1994-01-01 00:00:00
abstract：:Growth in the apical elongation zone of plant roots is central to the development of functional root systems. Rates of root segmental elongation change from accelerating to decelerating as cell development proceeds from newly formed to fully elongated status. One of the primary variables regulating these changes in el...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-3474-y
更新日期：2004-10-01 00:00:00
abstract：:In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037027
更新日期：1995-03-01 00:00:00
abstract：:Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019477
更新日期：1996-07-01 00:00:00
abstract：:The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041405
更新日期：1996-12-01 00:00:00
abstract：KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0516-1
更新日期：2016-10-01 00:00:00
abstract：:Isoprene synthase (ISPS) catalyzes the formation of isoprene, an important volatile terpenoid with strong effects on global atmospheric chemistry and protective physiological functions in plant leaves. Many terpene synthase genes including isoprene synthase, a member of the TPS-b cluster of this numerous gene family, ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9441-2
更新日期：2009-03-01 00:00:00
abstract：KEY MESSAGE:Jasmonic acid and RAP2.6L are induced upon wounding but are not involved in cell proliferation during healing in Arabidopsis hypocotyls. Plants produce jasmonic acid in response to wounding, but its role in healing, if any, has not been determined. Previously, the jasmonic acid-induced transcription factor,...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0702-4
更新日期：2018-04-01 00:00:00
abstract：:The colour of the red wine is essentially due to the release of anthocyanins from the red skin of grape berries during the process of wine making. Anthocyanins are synthesized during ripening of the berries under the control of VvMYBA1 transcription factor that controls the expression of UFGT. In order to identify the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9446-x
更新日期：2009-04-01 00:00:00
abstract：:Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017450
更新日期：1989-01-01 00:00:00
