Functional analysis of a recently originating, atypical presequence: mitochondrial import and processing of GUS fusion proteins in transgenic tobacco and yeast.

Abstract:

:A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tobacco, and the resultant GUS proteins were localized by cell fractionation. The amino-terminal 75 and 71 residues of RISP2 and RISP3, respectively, directed GUS import into mitochondria, where fusion protein processing occurred. The amino-terminal sequence of RISP4, which contains an atypical mitochondrial presequence, can translocate the GUS protein specifically into tobacco mitochondria with apparently low efficiency. Consistent with the proposal of a conserved mechanism for protein import in plants and fungi, the tobacco RISP3 and RISP4 presequences can direct import and processing of a GUS fusion protein in yeast mitochondria. Plant presequences, however, direct mitochondrial import in yeast less efficiently than the yeast presequence, indicating subtle differences between the plant and yeast mitochondrial import machineries. Our studies show that import of RISP4 may not require positively charged amino acid residues and an amphipathic secondary structure; however, these structural properties may improve the efficiency of mitochondrial import.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Huang J,Levings CS 3rd

doi

10.1007/BF00020982

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

519-33

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

29

pub_type

杂志文章
  • The trihelix transcription factor OsGTγ-2 is involved adaption to salt stress in rice.

    abstract:KEY MESSAGE:OsGTγ-2, a trihelix transcription factor, is a positive regulator of rice responses to salt stress by regulating the expression of ion transporters. Salinity stress seriously restricts rice growth and yield. Trihelix transcription factors (GT factors) specifically bind to GT elements and play a diverse role...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01010-1

    authors: Liu X,Wu D,Shan T,Xu S,Qin R,Li H,Negm M,Wu D,Li J

    更新日期:2020-07-01 00:00:00

  • Unusual sequence of an abscisic acid-inducible mRNA which accumulates late in Brassica napus seed development.

    abstract::We have analyzed the nucleotide sequence and accumulation of an mRNA which is prevalent in seeds of Brassica napus L. During normal development, the mRNA begins to accumulate during late embryogeny, is stored in dry seeds, and becomes undetectable in seedlings within 24 hours after imbibition. Moreover, abscisic acid ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017579

    authors: Harada JJ,Delisle AJ,Baden CS,Crouch ML

    更新日期:1989-04-01 00:00:00

  • Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1.

    abstract::The conserved protein CBF5, initially regarded as a centromere binding protein in yeast and higher plants, was later found within nucleoli and in Cajal bodies of yeast and metazoa. There, it is assumed to be involved in posttranscriptional pseudouridinylation of various RNA species that might be important for RNA proc...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-007-9226-z

    authors: Lermontova I,Schubert V,Börnke F,Macas J,Schubert I

    更新日期:2007-11-01 00:00:00

  • Molecular cloning, structure and expression of an elicitor-inducible chitinase gene from pine trees.

    abstract::We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005708611020

    authors: Wu H,Echt CS,Popp MP,Davis JM

    更新日期:1997-04-01 00:00:00

  • Vir-115 gene product is required to stabilize D1 translation intermediates in chloroplasts.

    abstract::The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00043874

    authors: Kim J,Klein PG,Mullet JE

    更新日期:1994-06-01 00:00:00

  • Expression of the Pib rice-blast-resistance gene family is up-regulated by environmental conditions favouring infection and by chemical signals that trigger secondary plant defences.

    abstract::The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1012457113700

    authors: Wang ZX,Yamanouchi U,Katayose Y,Sasaki T,Yano M

    更新日期:2001-11-01 00:00:00

  • Anaerobiosis-specific interaction of tobacco nuclear factors with cis-regulatory sequences in the maize GapC4 promoter.

    abstract::The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5' and 3' promoter deletions were linked to a chimeric GapC4 T...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006419232075

    authors: Geffers R,Cerff R,Hehl R

    更新日期:2000-05-01 00:00:00

  • Low temperatures impact dormancy status, flowering competence, and transcript profiles in crown buds of leafy spurge.

    abstract::Leafy spurge (Euphorbia esula) is an herbaceous perennial weed that produces vegetatively from an abundance of underground adventitious buds. In this study, we report the effects of different environmental conditions on vegetative production and flowering competence, and determine molecular mechanisms associated with ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9621-8

    authors: Doğramaci M,Horvath DP,Chao WS,Foley ME,Christoffers MJ,Anderson JV

    更新日期:2010-05-01 00:00:00

  • Puroindolines: the molecular genetic basis of wheat grain hardness.

    abstract::The variation in grain hardness is the single most important trait that determines end-use quality of wheat. Grain texture classification is based primarily on either the resistance of kernels to crushing or the particle size distribution of ground grain or flour. Recently, the molecular genetic basis of grain hardnes...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/a:1014837431178

    authors: Morris CF

    更新日期:2002-03-01 00:00:00

  • Transformation of carrot tissues derived from proembryogenic suspension cells: A useful model system for gene expression studies in plants.

    abstract::A method is described for the high frequency transformation of carrot proembryogenic suspension culture cells by a non-oncogenic Ti-plasmid vector (pGV3850::1103) which carried a chimaeric kanamycin resistance gene (nos-NPT-II). Plants were regenerated efficiently from transformed material by somatic embryogenesis in ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00015034

    authors: Scott RJ,Draper J

    更新日期:1987-05-01 00:00:00

  • The precursor of the F1beta subunit of the ATP synthase is covalently modified upon binding to plant mitochondrial.

    abstract::We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006375123496

    authors: von Stedingk E,Pavlov PF,Grinkevich VA,Glaser E

    更新日期:1999-11-01 00:00:00

  • An in silico strategy identified the target gene candidates regulated by dehydration responsive element binding proteins (DREBs) in Arabidopsis genome.

    abstract::Identification of downstream target genes of stress-relating transcription factors (TFs) is desirable in understanding cellular responses to various environmental stimuli. However, this has long been a difficult work for both experimental and computational practices. In this research, we presented a novel computationa...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9414-5

    authors: Wang S,Yang S,Yin Y,Guo X,Wang S,Hao D

    更新日期:2009-01-01 00:00:00

  • Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes.

    abstract::The role of the cell wall hydrolase polygalacturonase (PG) during fruit ripening was investigated using novel mutant tomato lines in which expression of the PG gene has been down regulated by antisense RNA. Tomato plants were transformed with chimaeric genes designed to express anti-PG RNA constitutively. Thirteen tra...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028773

    authors: Smith CJ,Watson CF,Morris PC,Bird CR,Seymour GB,Gray JE,Arnold C,Tucker GA,Schuch W,Harding S

    更新日期:1990-03-01 00:00:00

  • Molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types.

    abstract::HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern ana...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1022324920316

    authors: Agarwal M,Sahi C,Katiyar-Agarwal S,Agarwal S,Young T,Gallie DR,Sharma VM,Ganesan K,Grover A

    更新日期:2003-03-01 00:00:00

  • Isolation of a novel Arabidopsis ozone-induced cDNA by differential display.

    abstract::We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to isolate cDNAs corresponding to transcripts that accumulate in ozone-treated Arabidopsis thaliana. In this report we describe the characterization of an ozone-induced transcript, AtOZI1. AtOZI1 mRNA in untreated plants wa...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019121

    authors: Sharma YK,Davis KR

    更新日期:1995-10-01 00:00:00

  • Intron sequences that stimulate gene expression in Arabidopsis.

    abstract:KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0516-1

    authors: Rose AB,Carter A,Korf I,Kojima N

    更新日期:2016-10-01 00:00:00

  • Quantitative variation in components of the maize mitochondrial genome between tissues and between plants with different male-sterile cytoplasms.

    abstract::The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF02418241

    authors: Abbott AG,O'Dell M,Flavell RB

    更新日期:1985-07-01 00:00:00

  • Gene regulation in planta by plant-derived engineered zinc finger protein transcription factors.

    abstract::The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-7820-x

    authors: Holmes-Davis R,Li G,Jamieson AC,Rebar EJ,Liu Q,Kong Y,Case CC,Gregory PD

    更新日期:2005-02-01 00:00:00

  • Photosynthetic electron transport controls degradation but not production of psbA transcripts in the cyanobacterium Synechocystis 6803.

    abstract::Accumulation and stability of psbA and rbcL-S transcripts in Synechocystis 6803 was followed in the presence and absence of the photosynthesis inhibitors DCMU and methylviologen. Our results demonstrate that both transcript production and transcript stability are important regulatory elements for psbA gene expression ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00015080

    authors: Mohamed A,Jansson C

    更新日期:1991-05-01 00:00:00

  • Ordered assembly of mitochondria during rice germination begins with pro-mitochondrial structures rich in components of the protein import apparatus.

    abstract::Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound pro-mitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48 h following imbibition, an ordered increase in the abundance of transcripts e...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-3688-7

    authors: Howell KA,Millar AH,Whelan J

    更新日期:2006-01-01 00:00:00

  • Hygromycin B-induced cell death is partly mediated by reactive oxygen species in rice (Oryza sativa L.).

    abstract::The aminoglycoside antibiotic hygromycin B (Hyg) inhibits prokaryotic, chloroplast and mitochondrial protein synthesis. Because of the toxic effect of Hyg on plant cells, the HPT gene, encoding hygromycin phosphotransferase, has become one of the most widely used selectable markers in plant transformation. Yet the mec...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0380-4

    authors: Oung HM,Lin KC,Wu TM,Chandrika NN,Hong CY

    更新日期:2015-12-01 00:00:00

  • EGY1 plays a role in regulation of endodermal plastid size and number that are involved in ethylene-dependent gravitropism of light-grown Arabidopsis hypocotyls.

    abstract::Egy1 was isolated as an ethylene-dependent gravitropism-deficient Arabidopsis mutant. Molecular studies reveal that EGY1 gene encodes a 59-kDa plastid-targeted metalloprotease. It is actively expressed in hypocotyl tissue and targets to endodermal and cortex plastid. Its protein level is up-regulated by both ethylene ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-007-9273-5

    authors: Guo D,Gao X,Li H,Zhang T,Chen G,Huang P,An L,Li N

    更新日期:2008-03-01 00:00:00

  • The two genes for the small subunit of RuBP Carboxylase/oxygenase are closely linked in Chlamydomonas reinhardtii.

    abstract::Ribulose bisphosphate carboxylase-oxygenase (Rubisco) is a key enzyme in the photosynthetic fixation of CO2 by the chloroplast. The synthesis of the enzyme is an example of the cooperation between the chloroplast and the nucleocytoplasmic compartments, as it is assembled from subunits encoded in the two respective gen...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021302

    authors: Goldschmidt-Clermont M

    更新日期:1986-01-01 00:00:00

  • Conservation of rice genetic resources: the role of the International Rice Genebank at IRRI.

    abstract::Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:

    authors: Jackson MT

    更新日期:1997-09-01 00:00:00

  • Functional analysis of the grapevine paralogs of the SMG7 NMD factor using a heterolog VIGS-based gene depletion-complementation system.

    abstract::Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9726-0

    authors: Benkovics AH,Nyikó T,Mérai Z,Silhavy D,Bisztray GD

    更新日期:2011-02-01 00:00:00

  • Specific interaction of the tomato bZIP transcription factor VSF-1 with a non-palindromic DNA sequence that controls vascular gene expression.

    abstract::The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006030007333

    authors: Ringli C,Keller B

    更新日期:1998-08-01 00:00:00

  • Coordinate modulation of maize sulfate permease and ATP sulfurylase mRNAs in response to variations in sulfur nutritional status: stereospecific down-regulation by L-cysteine.

    abstract::To gain insight into the regulatory mechanisms and the signals responsible for the adaptation of higher plants to conditions of varying sulfate availability, we have isolated from a sulfate-deprived root library maize cDNAs encoding sulfate permease (ZmST1) and ATP sulfurylase (ZmAS1), the two earliest components of t...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006148815106

    authors: Bolchi A,Petrucco S,Tenca PL,Foroni C,Ottonello S

    更新日期:1999-02-01 00:00:00

  • mRNA transcription profile of potato (Solanum tuberosum L.) exposed to ultrasound during different stages of in vitro plantlet development.

    abstract:KEY MESSAGE:In response to an ultrasound pulse, several hundred DEGs, including in response to stress, were up- or down-regulated in in vitro potato plantlets. Despite this abiotic stress, plantlets survived. Ultrasound (US) can influence plant growth and development. To better understand the genetic mechanism underlyi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00876-0

    authors: Dobránszki J,Hidvégi N,Gulyás A,Teixeira da Silva JA

    更新日期:2019-07-01 00:00:00

  • Markers for hypersensitive response and senescence show distinct patterns of expression.

    abstract::Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily obs...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006133311402

    authors: Pontier D,Gan S,Amasino RM,Roby D,Lam E

    更新日期:1999-04-01 00:00:00

  • Direct interaction between the tobacco mosaic virus helicase domain and the ATP-bound resistance protein, N factor during the hypersensitive response in tobacco plants.

    abstract::Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-5817-8

    authors: Ueda H,Yamaguchi Y,Sano H

    更新日期:2006-05-01 00:00:00