Abstract：
:The role of the cell wall hydrolase polygalacturonase (PG) during fruit ripening was investigated using novel mutant tomato lines in which expression of the PG gene has been down regulated by antisense RNA. Tomato plants were transformed with chimaeric genes designed to express anti-PG RNA constitutively. Thirteen transformed lines were obtained of which five were analysed in detail. All contained a single PG antisense gene, the expression of which led to a reduction in PG enzyme activity in ripe fruit to between 5% and 50% that of normal. One line, GR16, showed a reduction to 10% of normal PG activity. The reduction in activity segregated with the PG antisense gene in selfed progeny of GR16. Plants homozygous for the antisense gene showed a reduction of PG enzyme expression of greater than 99%. The PG antisense gene was inherited stably through two generations. In tomato fruit with a residual 1% PG enzyme activity pectin depolymerisation was inhibited, indicating that PG is involved in pectin degradation in vivo. Other ripening parameters, such as ethylene production, lycopene accumulation, polyuronide solubilisation, and invertase activity, together with pectinesterase activity were not affected by the expression of the antisense gene.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Smith CJ,Watson CF,Morris PC,Bird CR,Seymour GB,Gray JE,Arnold C,Tucker GA,Schuch W,Harding Sdoi
10.1007/BF00028773subject
Has Abstract,Author List Incompletepub_date
1990-03-01 00:00:00pages
369-79issue
3eissn
0167-4412issn
1573-5028journal_volume
14pub_type
杂志文章abstract：:Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photo...
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pub_type： 杂志文章
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更新日期：1988-07-01 00:00:00
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更新日期：1990-12-01 00:00:00
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