Heat-inducible hygromycin resistance in transgenic tobacco.

Abstract:

:We have constructed a chimaeric gene consisting of the promoter of the soybean heat shock (hs) gene Gmhsp17, 6-L, the coding region of a hygromycin phosphotransferase (hpt) gene, and the termination sequence of the nopaline synthase (nos) gene. This gene fusion was introduced into tobacco by Agrobacterium-mediated gene transfer. Heat-inducible synthesis of mRNA was shown by northern hybridization, and translation of this RNA into a functional protein was indicated by plant growth on hygromycin-containing media in a temperature-dependent fashion. One hour incubation at 40 degrees C per day, applied for several weeks, was sufficient to express the resistant phenotype in transgenic plants containing the chimaeric hs-hpt gene. These data suggest that the hygromycin resistance gene is functional and faithfully controlled by the soybean hs promoter. The suitability of these transgenic plants for selection of mutations that alter the hs response is discussed.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Severin K,Schöffl F

doi

10.1007/BF00039423

subject

Has Abstract

pub_date

1990-12-01 00:00:00

pages

827-33

issue

6

eissn

0167-4412

issn

1573-5028

journal_volume

15

pub_type

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