Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress.

Abstract:

:A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 49.4% and 58.5% identity with glyoxalase-I isolated from bacteria and human, respectively. Furthermore, yeast cells expressing GLX1 showed a glyoxalase-I specific activity 20-fold higher than non-transformed cells. Both GLX1 mRNA and glyoxalase-I polypeptide levels increased 2- to 3-fold in roots, stems and leaves of plants treated with either NaCl, mannitol, or abscisic acid. Immunohistochemical localization indicated that glyoxalase-I was expressed in all cell types, with preferential accumulation in phloem sieve elements. This expression pattern was not appreciably altered by salt-stress. We suggest that the increased expression of glyoxalase-I may be linked to a higher demand for ATP generation and to enhanced glycolysis in salt-stressed plants.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Espartero J,Sánchez-Aguayo I,Pardo JM

doi

10.1007/BF00020464

subject

Has Abstract

pub_date

1995-12-01 00:00:00

pages

1223-33

issue

6

eissn

0167-4412

issn

1573-5028

journal_volume

29

pub_type

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