'Phytoantibodies': a general vector for the expression of immunoglobulin domains in transgenic plants.

abstract：:We have cloned and sequenced a gene, pds, from the cyanobacterium Synechococcus PCC7942 that is responsible for resistance to the bleaching herbicide norflurazon. A point mutation in that gene, leading to an amino acid substitution from valine to glycine in its polypeptide product, was found to confer this resistance....

journal_title：Plant molecular biology

authors： Chamovitz D,Pecker I,Hirschberg J

更新日期：1991-06-01 00:00:00

abstract：:β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α',...

journal_title：Plant molecular biology

authors： Tsubokura Y,Hajika M,Kanamori H,Xia Z,Watanabe S,Kaga A,Katayose Y,Ishimoto M,Harada K

更新日期：2012-02-01 00:00:00

abstract：:Transgenic plants carrying chimeric genes composed of segments of the 5'-flanking region of the Arabidopsis 9-tubulin gene (TUB9) fused to the coding region of the beta-glucuronidase (GUS) gene of Escherichia coli were used to investigate the temporal and spatial patterns of TUB9 expression. Chimeric genes that contai...

journal_title：Plant molecular biology

authors： Cheng Z,Snustad DP,Carter JV

更新日期：2001-10-01 00:00:00

abstract：:With the completion of genomic sequencing of rice, rice has been firmly established as a model organism for both basic and applied research. The next challenge is to uncover the functions of genes predicted by sequence analysis. Considering the amount of effort and the diversity of disciplines required for functional ...

journal_title：Plant molecular biology

authors： Hirochika H,Guiderdoni E,An G,Hsing YI,Eun MY,Han CD,Upadhyaya N,Ramachandran S,Zhang Q,Pereira A,Sundaresan V,Leung H

更新日期：2004-02-01 00:00:00

abstract：KEY MESSAGE:To explore the function of Dof transcription factors during kernel development in maize, we first identified Dof genes in the maize genome. We found that ZmDof3 was exclusively expressed in the endosperm of maize kernel and had the features of a Dof transcription factor. Suppression of ZmDof3 resulted in a ...

journal_title：Plant molecular biology

authors： Qi X,Li S,Zhu Y,Zhao Q,Zhu D,Yu J

更新日期：2017-01-01 00:00:00

abstract：:Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of ...

journal_title：Plant molecular biology

authors： Hellens RP,Edwards EA,Leyland NR,Bean S,Mullineaux PM

更新日期：2000-04-01 00:00:00

abstract：:Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...

journal_title：Plant molecular biology

authors： Esfandiari E,Jin Z,Abdeen A,Griffiths JS,Western TL,Haughn GW

更新日期：2013-01-01 00:00:00

abstract：:During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...

journal_title：Plant molecular biology

authors： Torrent M,Alvarez I,Geli MI,Dalcol I,Ludevid D

更新日期：1997-05-01 00:00:00

abstract：:A homologue of the multidrug resistance (MDR) gene was obtained while screening a potato stolon tip cDNA expression library with 35S-labeled calmodulin. The mammalian MDR gene codes for a membrane-bound P-glycoprotein (170-180 kDa) which imparts multidrug resistance to cancerous cells. The potato cDNA (PMDR1) codes fo...

journal_title：Plant molecular biology

authors： Wang W,Takezawa D,Poovaiah BW

更新日期：1996-06-01 00:00:00

abstract：:The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...

journal_title：Plant molecular biology

authors： Wang SM,Lue WL,Eimert K,Chen J

更新日期：1996-08-01 00:00:00

abstract：:In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...

journal_title：Plant molecular biology

authors： Showalter AM,Butt AD,Kim S

更新日期：1992-05-01 00:00:00

abstract：:Alterations in gene expression during early stages of dormancy release in grapevine buds were analyzed to facilitate the identification of gene products that may mediate the signal transduction of a dormancy-release signal, or derepression of meristematic activity. In the present report we describe the identification ...

journal_title：Plant molecular biology

authors： Or E,Vilozny I,Eyal Y,Ogrodovitch A

更新日期：2000-07-01 00:00:00

abstract：:Primer extension was used to characterize alpha-amylase mRNAs from aleurone tissue of barley (Hordeum vulgare L. cv. Himalaya) grains. Two synthetic oligonucleotides, specific for the low-pI and high-pI alpha-amylase groups, were used as primers for synthesis of cDNA from total RNA preparations. Between them, these tw...

journal_title：Plant molecular biology

authors： Chandler PM,Huiet L

更新日期：1991-04-01 00:00:00

abstract：:The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there ...

journal_title：Plant molecular biology

authors： Turek I,Gehring C

更新日期：2016-06-01 00:00:00

abstract：:Two full-length cDNA clones (faEG1 and faEG3, respectively) have been isolated by screening a cDNA library representing transcripts from red strawberry fruits. Southern blot analysis of genomic DNA suggests that the strawberry endo-beta-1,4-glucanases (EGases) are encoded by a multigene family. The cognate genes are p...

journal_title：Plant molecular biology

authors： Trainotti L,Spolaore S,Pavanello A,Baldan B,Casadoro G

更新日期：1999-05-01 00:00:00

abstract：:The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...

journal_title：Plant molecular biology

authors： Walther-Larsen H,Brandt J,Collinge DB,Thordal-Christensen H

更新日期：1993-03-01 00:00:00

abstract：:Leafy spurge (Euphorbia esula) is an herbaceous perennial weed that produces vegetatively from an abundance of underground adventitious buds. In this study, we report the effects of different environmental conditions on vegetative production and flowering competence, and determine molecular mechanisms associated with ...

journal_title：Plant molecular biology

authors： Doğramaci M,Horvath DP,Chao WS,Foley ME,Christoffers MJ,Anderson JV

更新日期：2010-05-01 00:00:00

abstract：:In order to analyse the cruciferin gene family in radish a cDNA library was screened either with heterologous rapeseed probes or by differential screening and sequencing. We could identify six partial cDNA clones belonging to two different groups of cruciferin genes which do not cross-hybridize, and probably three dis...

journal_title：Plant molecular biology

authors： Depigny-This D,Raynal M,Aspart L,Delseny M,Grellet F

更新日期：1992-11-01 00:00:00

abstract：:Using RNA from developing cotyledons and leaves of bean two products were synthesizedin vitro, with Mr 31000 and 31500 which are antigenically related to the subunit of phytoferritin (Mr 26500). The relative abundance of the two products is dependent on the type of tissue from which the RNA was derived. Evidence is pr...

journal_title：Plant molecular biology

authors： van der Mark F,Klerk H,Bienfait F

更新日期：1983-11-01 00:00:00

abstract：:We have compared physical and genetic maps of the region around the legJ gene in pea. In this vicinity there are four B-type legumin genes, arranged as two close pairs. The detection of a recombination event within this gene cluster allows the orientation of this group of genes within the surrounding linkage group to ...

journal_title：Plant molecular biology

authors： Turner L,Hellens RP,Lee D,Ellis TH

更新日期：1993-04-01 00:00:00

abstract：:We have constructed a chimaeric gene consisting of the promoter of the soybean heat shock (hs) gene Gmhsp17, 6-L, the coding region of a hygromycin phosphotransferase (hpt) gene, and the termination sequence of the nopaline synthase (nos) gene. This gene fusion was introduced into tobacco by Agrobacterium-mediated gen...

journal_title：Plant molecular biology

authors： Severin K,Schöffl F

更新日期：1990-12-01 00:00:00

abstract：:Isoprene synthase (ISPS) catalyzes the formation of isoprene, an important volatile terpenoid with strong effects on global atmospheric chemistry and protective physiological functions in plant leaves. Many terpene synthase genes including isoprene synthase, a member of the TPS-b cluster of this numerous gene family, ...

journal_title：Plant molecular biology

authors： Cinege G,Louis S,Hänsch R,Schnitzler JP

更新日期：2009-03-01 00:00:00

abstract：:Young pine seedlings respond to environmental stress by induced synthesis of pinosylvin, a stilbene phytoalexin. Heartwood of pine trees is characterized by a high content of pinosylvin. The formation of pinosylvin from cinnamoyl-CoA and three molecules malonyl-CoA catalysed by pinosylvin synthase is typical of the ge...

journal_title：Plant molecular biology

authors： Preisig-Müller R,Schwekendiek A,Brehm I,Reif HJ,Kindl H

更新日期：1999-01-01 00:00:00

abstract：:A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-...

journal_title：Plant molecular biology

authors： Zhou CJ,Li J,Zou JC,Liang FS,Ye CJ,Jin DM,Weng ML,Wang B

更新日期：2006-02-01 00:00:00

abstract：:In this study, attempt has been made to produce a selected cultivar of tea with low-caffeine content using RNAi technology. The caffeine biosynthetic pathway in tea has been proposed to involve three N-methyltransferases such as xanthosine methyltransferase, 7-N-methylxanthine methyltransferase and 3, 7-dimethylxanthi...

journal_title：Plant molecular biology

authors： Mohanpuria P,Kumar V,Ahuja PS,Yadav SK

更新日期：2011-08-01 00:00:00

abstract：:The chloroplast gene for the epsilon subunit (atpE) of the CF1/CF0 ATPase in the green alga Chlamydomonas reinhardtii has been localized and sequenced. In contrast to higher plants, the atpE gene does not lie at the 3' end of the beta subunit (atpB) gene in the chloroplast genome of C. reinhardtii, but is located at a...

journal_title：Plant molecular biology

authors： Woessner JP,Gillham NW,Boynton JE

更新日期：1987-03-01 00:00:00

abstract：:Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...

journal_title：Plant molecular biology

authors： Jackson MT

更新日期：1997-09-01 00:00:00

abstract：:Unilateral incompatibility often occurs between self-incompatible (SI) species and their self-compatible (SC) relatives. For example, SI Nicotiana alata rejects pollen from SC N. plumbaginifolia, but the reciprocal pollination is compatible. This interspecific pollen rejection system closely resembles intraspecific S-...

journal_title：Plant molecular biology

authors： Beecher B,Murfett J,McClure BA

更新日期：1998-03-01 00:00:00

abstract：:A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...

journal_title：Plant molecular biology

authors： Bak S,Kahn RA,Nielsen HL,Moller BL,Halkier BA

更新日期：1998-02-01 00:00:00

abstract：:The identification of a cDNA (MR19) corresponding to a maize alpha-tubulin and homologous genomic clones (MG19/6 and MG19/14) is described. The cDNA has been isolated by differential screening of a cDNA maize root library. We have found two alpha-tubulin genes in a tandem arrangement in the genomic clones, separated b...

journal_title：Plant molecular biology

authors： Montoliu L,Rigau J,Puigdomènech P

更新日期：1990-01-01 00:00:00