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Primer extension studies on alpha-amylase mRNAs in barley aleurone. I. Characterization and quantification of the transcripts.

Abstract：

:Primer extension was used to characterize alpha-amylase mRNAs from aleurone tissue of barley (Hordeum vulgare L. cv. Himalaya) grains. Two synthetic oligonucleotides, specific for the low-pI and high-pI alpha-amylase groups, were used as primers for synthesis of cDNA from total RNA preparations. Between them, these two oligonucleotides appear to account for all major alpha-amylase mRNAs as judged by hybrid-arrested translation of alpha-amylase mRNAs in a cell-free system. Reconstruction experiments indicated that the levels of extended primers (determined by scintillation counting) were directly proportional to the level of input mRNA over a wide range. This indicates that the technique is suitable for quantification of relative levels of individual alpha-amylase from approximately 2% to 100% of maximal levels. The nucleotide sequences of extended primers defined two different alpha-amylase mRNAs in each of the low-pI and high-pI groups, and possibly a third mRNA in the high-pI group.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Chandler PM,Huiet L

doi

10.1007/BF00023427

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

627-35

issue

4

eissn

0167-4412

issn

1573-5028

journal_volume

16

pub_type

杂志文章

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