Abstract:
:An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Riccardi G,De Rossi E,Della Valle G,Ciferri Odoi
10.1007/BF02418760subject
Has Abstractpub_date
1985-03-01 00:00:00pages
133-6issue
2-3eissn
0167-4412issn
1573-5028journal_volume
4pub_type
杂志文章abstract::We have compared physical and genetic maps of the region around the legJ gene in pea. In this vicinity there are four B-type legumin genes, arranged as two close pairs. The detection of a recombination event within this gene cluster allows the orientation of this group of genes within the surrounding linkage group to ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00038999
更新日期:1993-04-01 00:00:00
abstract::Glyoxalase II is part of the glutathione-dependent glyoxalase detoxification system. In addition to its role in the detoxification of cytotoxic 2-oxo-aldehydes, specifically methylglyoxal, it has been suggested that the glyoxalase system may also play a role in controlling cell differentiation and proliferation. Durin...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005891123344
更新日期:1997-11-01 00:00:00
abstract::A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich protein...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023430
更新日期:1991-04-01 00:00:00
abstract::Lectin receptor-like kinases (LecRLKs) are members of RLK family composed of lectin-like extracellular recognition domain, transmembrane domain and cytoplasmic kinase domain. LecRLKs are plasma membrane proteins believed to be involved in signal transduction. However, most of the members of the protein family even in ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0319-9
更新日期:2015-05-01 00:00:00
abstract::Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015816
更新日期:1987-09-01 00:00:00
abstract::MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0156-7
更新日期:2014-03-01 00:00:00
abstract::Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9578-7
更新日期:2010-03-01 00:00:00
abstract::Expression of the chloroplast electron shuttle ferredoxin is induced by light through mechanisms that partially depend on sequences lying in the coding region of the gene, complicating its manipulation by promoter engineering. Ferredoxin expression is also down-regulated under virtually all stress situations, and it i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9786-9
更新日期:2011-08-01 00:00:00
abstract::Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::We have characterized the topoisomerase I and II activities in nuclear extracts from immature embryos of Zea mays and the effect of the treatment with 2,4-dichlorophenoxyacetic acid (2,4-D) and abscisic acid (ABA). These extracts were shown to be essentially devoid of protease and nuclease activities and they were tes...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017917
更新日期:1991-01-01 00:00:00
abstract::Two soybean ribulose-1,5-bisphosphate carboxylase small subunit (SSU) genes, SRS1 and SRS4, are highly homologous over a region that includes 4 kb of 5' and 1 kb of 3' flanking sequences. The expression of these genes was compared using synthetic oligonucleotide probes. Analysis of a soybean leaf cDNA library indicate...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019389
更新日期:1990-06-01 00:00:00
abstract::Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 10(5) copies...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041174
更新日期:1995-11-01 00:00:00
abstract::We isolated a rice cDNA encoding nucleoside diphosphate kinase (NDK, EC 2.7.4.6). The deduced amino acid sequence of the rice NDK shows highest homology to spinach NDK-I. The rice NDK gene exhibits a strong codon bias (73.8% GC) in the third position of the codon. DNA blot analysis indicated that at least single NDK g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021824
更新日期:1993-12-01 00:00:00
abstract::In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0320-3
更新日期:2015-06-01 00:00:00
abstract:KEY MESSAGE:IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well st...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01114-8
更新日期:2021-01-22 00:00:00
abstract::Target sequence cleavage is the essential step for intron invasion into an intronless allele. DNA cleavage at a specific site is performed by an endonuclease, termed a homing enzyme, which is encoded by an open reading frame within the intron. The recognition properties of them have only been analyzed in vitro, using ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9009-y
更新日期:2006-09-01 00:00:00
abstract::Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-lengt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9281-5
更新日期:2008-03-01 00:00:00
abstract::A MADS-box gene was isolated using the suppressive subtractive hybridization library between early-flowering mutant and wild-type trifoliate orange (Poncirus trifoliata L. Raf.). This gene is highly homologous with Arabidopsis SHORT VEGETATIVE PHASE (SVP). Based on real-time PCR and in situ hybridization during bud di...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9660-1
更新日期:2010-09-01 00:00:00
abstract::Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specif...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021807
更新日期:1996-05-01 00:00:00
abstract::As an important osmoprotectant, glycine betaine (GB) plays an essential role in resistance to abiotic stress in a variety of organisms, including rice (Oryza sativa L.). However, GB content is too low to be detectable in rice, although rice genome possesses several orthologs coding for betaine aldehyde dehydrogenase (...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0239-0
更新日期:2014-11-01 00:00:00
abstract::The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP(+) oxidoreductase, E.C.: 1.5.1.3) from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a molecular weight of 183 000±2 500, composed of identical subunits of 58 400±1 000. The enzyme is only weakly ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037557
更新日期:1985-11-01 00:00:00
abstract:KEY MESSAGE:1599 novel circRNAs and 1583 heat stress-specific circRNAs were identified in Arabidopsis. Heat stress enhanced accumulation of circRNAs remarkably. Heat stress altered the sizes of circRNAs, numbers of circularized exons and alterative circularization events. A putative circRNA-mediated ceRNA networks unde...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0684-7
更新日期:2018-02-01 00:00:00
abstract::A mutant of alfalfa mosaic virus (AMV), which in contrast to wild type (wt) can invade cowpea plants systemically, is replicated more efficiently in cowpea protoplasts than the wt. Mutant preparations isolated from infected cowpea protoplasts contained a higher amount of middle component (M, containing RNA 2) than wt ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF01595169
更新日期:1983-03-01 00:00:00
abstract::A number of A/T-rich sequences and a CATGCAT/A sequence are contained in the 5'-upstream regions of the genes encoding concanavalin A (Con A) and canavalin, two major seed storage proteins of Canavalia gladiata D.C. To study the role of these sequences in the seed-specific gene expression, we constructed 5'-deletion m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020226
更新日期:1995-02-01 00:00:00
abstract::The hypersensitive response (HR) involves programmed cell death (PCD) in response to pathogen infection. To investigate the pathogen resistance signaling pathway, we previously identified the Arabidopsis mutant cpr22, which displays constitutive activation of multiple defense responses including HR like cell death. Th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9239-7
更新日期:2007-12-01 00:00:00
abstract::We have previously shown that two CRE elements situated on a 31 bp region of the cauliflower mosaic virus (CaMV) 35S promoter activate gene expression in the yeast Saccharomyces cerevisiae and are regulated by cAMP. Studies with the yeast transcription factors GCN4, SKO1 and YAP1, which bind CRE-like sequences, showed...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023248
更新日期:1994-05-01 00:00:00
abstract::Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-1303-y
更新日期:2004-07-01 00:00:00
abstract::The complete nucleotide sequence of the rDNA large spacer region was determined for two related legume plants, Vicia faba and Pisum sativum. In the large spacers of both plants, there were one or several tandem arrays of repeated sequences which account well for the length heterogeneity of the rDNA repeating units. Wh...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019395
更新日期:1990-06-01 00:00:00
abstract::A vector has been designed that contains a truncated CaMV (cauliflower mosaic virus) 35S promoter fused to a receptor gene encoding beta-glucuronidase (GUS), placed adjacent to the left border sequence of an Agrobacterium vector. In potato plants transformed with this vector, different patterns of transcription were d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020557
更新日期:1991-02-01 00:00:00
abstract::Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022502501373
更新日期:2003-03-01 00:00:00