Abstract:
:Glyoxalase II is part of the glutathione-dependent glyoxalase detoxification system. In addition to its role in the detoxification of cytotoxic 2-oxo-aldehydes, specifically methylglyoxal, it has been suggested that the glyoxalase system may also play a role in controlling cell differentiation and proliferation. During the analysis of a T-DNA-tagged mutant of Arabidopsis we identified the gene for a glyoxalase II isozyme (GLY1) that appears to be mitochondrially localized. The cDNA encoding a glyoxalase II cytoplasmic isozyme (GLY2) was also isolated and characterized. Southern blot and sequence analyses indicate that glyoxalase II proteins are encoded by at least two multigene families in Arabidopsis. Escherichia coli cells expressing either GLY1 or GLY2 exhibit increased glyoxalase II activity, confirming that they do, in fact, encode glyoxalase II proteins. Northern analysis shows that the two genes are differentially expressed. Transcripts for the mitochondrial isozyme are most abundant in roots, while those for the cytoplasmic isozyme are highest in flower buds. The identification of glyoxalase II isozymes that are differentially expressed suggests that they may play different roles in the cell.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Maiti MK,Krishnasamy S,Owen HA,Makaroff CAdoi
10.1023/a:1005891123344subject
Has Abstractpub_date
1997-11-01 00:00:00pages
471-81issue
4eissn
0167-4412issn
1573-5028journal_volume
35pub_type
杂志文章abstract::Vernalization, the requirement of a period of low temperature to induce transition from the vegetative to reproductive state, is an evolutionarily and economically important trait in the Triticeae. The genetic basis of vernalization in cultivated barley (Hordeum vulgare subsp. vulgare) can be defined using the two-loc...
journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020388
更新日期:1995-06-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:2000-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017731
更新日期:1990-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1994-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9726-0
更新日期:2011-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006493021557
更新日期:2001-01-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010637012528
更新日期:2001-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2008-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1991-12-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016504
更新日期:1990-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0605-9
更新日期:2017-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2001-12-01 00:00:00