High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda).

abstract：:Gametophytic apomictic plants form non-reduced embryo sacs that generate clonal embryos by parthenogenesis, in the absence of both meiosis and egg-cell fertilization. Here we report the sequence and expression analysis of a lorelei-like Paspalum notatum gene, n20gap-1, which encodes a GPI-anchored protein previously a...

journal_title：Plant molecular biology

authors： Felitti SA,Seijo JG,González AM,Podio M,Laspina NV,Siena L,Ortiz JP,Pessino SC

更新日期：2011-11-01 00:00:00

abstract：:Golgins are a family of coiled-coil proteins that are associated with the Golgi apparatus. They are necessary for tethering events in membrane fusion and may act as structural support for Golgi cisternae. Here we report on the identification of an Arabidopsis golgin which is a homologue of CASP, a known transmembrane ...

journal_title：Plant molecular biology

authors： Renna L,Hanton SL,Stefano G,Bortolotti L,Misra V,Brandizzi F

更新日期：2005-05-01 00:00:00

abstract：:DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glycine max (L.) Merr.). The inheritance of AFLPs was studied using a cross between the ancestral Glycine soja PI4...

journal_title：Plant molecular biology

authors： Prabhu RR,Gresshoff PM

更新日期：1994-10-01 00:00:00

abstract：KEY MESSAGE:AcGST1, an anthocyanin-related GST, may functions as a carrier to transport anthocyanins from ER to tonoplast in kiwifruit. It was positively regulated by AcMYBF110 through directly binding to its promoter. Anthocyanins are synthesized in the cytoplasmic surface of the endoplasmic reticulum but accumulate p...

journal_title：Plant molecular biology

authors： Liu Y,Qi Y,Zhang A,Wu H,Liu Z,Ren X

更新日期：2019-07-01 00:00:00

abstract：:R2R3-MYB genes play a pivotal role in regulating anthocyanin accumulation. Here, we report two tandemly duplicated R2R3-MYB genes in peach, PpMYB10.1 and PpMYB10.2, with the latter showing lower ability to induce anthocyanin accumulation than the former. Site-directed mutation assay revealed two amino acid changes in ...

journal_title：Plant molecular biology

authors： Zhou H,Liao L,Xu S,Ren F,Zhao J,Ogutu C,Wang L,Jiang Q,Han Y

更新日期：2018-09-01 00:00:00

abstract：:In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...

journal_title：Plant molecular biology

authors： Piechota J,Bereza M,Sokołowska A,Suszyński K,Lech K,Jańska H

更新日期：2015-06-01 00:00:00

abstract：:In plants, cytosolic Ca2+ levels are tightly regulated, and changes in cytosolic Ca2+ have been implicated in converting numerous signals into adapted responses. Vacuolar ion transporters are thought to be key mediators of cytosolic Ca2+ concentrations. In an attempt to interpret the role of vacuolar Ca2+ transport in...

journal_title：Plant molecular biology

authors： Hirschi KD,Miranda ML,Wilganowski NL

更新日期：2001-05-01 00:00:00

abstract：:A physical mapping method for unique nucleotide sequences on specific chromosomal regions was developed combining objective chromosome identification and highly sensitive fluorescence in situ hybridisation (FISH). Four unique nucleotide sequences cloned from rice genomic DNAs, varying in size from 1.3 to 400 kb, were ...

journal_title：Plant molecular biology

authors： Ohmido N,Akiyama Y,Fukui K

更新日期：1998-12-01 00:00:00

abstract：:The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...

journal_title：Plant molecular biology

authors： Rubinstein B

更新日期：2000-10-01 00:00:00

abstract：:Transcription factors play an important role in regulating gene expression in response to stress and pathogen tolerance. We describe here that overexpression of an ERF/AP2 pepper transcription factor (CaPF1) in transgenic Virginia pine (Pinus virginiana Mill.) confers tolerance to heavy metals Cadmium, Copper, and Zin...

journal_title：Plant molecular biology

authors： Tang W,Charles TM,Newton RJ

更新日期：2005-11-01 00:00:00

abstract：:The genetics of male fertility restoration and the RFLP of mitochondrial DNA were studied for 16 sunflower cytoplasms (15 male-sterile and a male-fertile). Male fertility restoration/male sterility maintenance patterns distinguished 12 cytotypes. Four cytoplasms were completely unrestored so they were not distinguishe...

journal_title：Plant molecular biology

authors： Crouzillat D,de la Canal L,Perrault A,Ledoigt G,Vear F,Serieys H

更新日期：1991-03-01 00:00:00

abstract：:Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a m...

journal_title：Plant molecular biology

authors： Irish EE,McMurray D

更新日期：2006-03-01 00:00:00

abstract：:Two S-adenosylmethionine synthetase (SAMS) cDNAs, PcSAMS1 and PcSAMS2, have been identified in Pinus contorta. We found that the two genes are differentially expressed during root development. Thus, PcSAMS1 is preferentially expressed in roots and exhibits a specific expression pattern in the meristem at the onset of ...

journal_title：Plant molecular biology

authors： Lindroth AM,Saarikoski P,Flygh G,Clapham D,Grönroos R,Thelander M,Ronne H,von Arnold S

更新日期：2001-06-01 00:00:00

abstract：:The folding and assembly of proteins in cells often requires the assistance of molecular chaperones such as the Hsp70 and Hsp60 heat shock proteins. Hsp70 chaperones cooperate with DnaJ and GrpE homologues to ensure a productive folding cycle. In this study we describe the gene for the first chloroplast localized DnaJ...

journal_title：Plant molecular biology

authors： Schlicher T,Soll J

更新日期：1997-01-01 00:00:00

abstract：:The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...

journal_title：Plant molecular biology

authors： Phillips AL,Huttly AK

更新日期：1994-02-01 00:00:00

abstract：:Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...

journal_title：Plant molecular biology

authors： Ioset JR,Urbaniak B,Ndjoko-Ioset K,Wirth J,Martin F,Gruissem W,Hostettmann K,Sautter C

更新日期：2007-11-01 00:00:00

abstract：:The formation of auxin glucose conjugate is proposed to be one of the molecular modifications controlling auxin homeostasis. However, the involved mechanisms and relevant physiological significances are largely unknown or poorly understood. In this study, Arabidopsis UGT75D1 was at the first time identified to be an i...

journal_title：Plant molecular biology

authors： Zhang GZ,Jin SH,Jiang XY,Dong RR,Li P,Li YJ,Hou BK

更新日期：2016-01-01 00:00:00

abstract：:Expression of the chloroplast electron shuttle ferredoxin is induced by light through mechanisms that partially depend on sequences lying in the coding region of the gene, complicating its manipulation by promoter engineering. Ferredoxin expression is also down-regulated under virtually all stress situations, and it i...

journal_title：Plant molecular biology

authors： Ceccoli RD,Blanco NE,Medina M,Carrillo N

更新日期：2011-08-01 00:00:00

abstract：:The suppression of plant defence reactions plays a crucial role in causing plant diseases. In this report, we show that inducible plant defences are repressed during the development of Cercospora leaf spot disease. In the early phase of infection of sugar beet (Beta vulgaris L.) leaves with the phytopathogenic fungus ...

journal_title：Plant molecular biology

authors： Schmidt K,Heberle B,Kurrasch J,Nehls R,Stahl DJ

更新日期：2004-08-01 00:00:00

abstract：:Plant cells respond to cold stress via a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation. The C-repeat binding factor (CBF) signaling pathway is essential for cold response of flowering plants. Our previously study documented a novel CBF-like gene from the cold-tolerant Caps...

journal_title：Plant molecular biology

authors： Zhou M,Xu M,Wu L,Shen C,Ma H,Lin J

更新日期：2014-06-01 00:00:00

abstract：:As part of a strategy to determine the precise role of pea (Pisum sativum) lectin, Psl, in nodulation of pea by Rhizobium leguminosarum, mutations were introduced into the genetic determinant for pea lectin by site-directed mutagenesis using PCR. Introduction of a specific mutation, N125D, into a central area of the s...

journal_title：Plant molecular biology

authors： van Eijsden RR,Hoedemaeker FJ,Díaz CL,Lugtenberg BJ,de Pater BS,Kijne JW

更新日期：1992-12-01 00:00:00

abstract：:When subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (BADH). We report here cloning and characterization of BADH cDNA fro...

journal_title：Plant molecular biology

authors： Ishitani M,Nakamura T,Han SY,Takabe T

更新日期：1995-01-01 00:00:00

abstract：:Arabidopsis thaliana encodes four Dicer-like (DCL) proteins and five dsRNA-binding (DRB) proteins. We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo. The phenotype of a mutant with a defect in DCL4 (dcl4-2) was similar to...

journal_title：Plant molecular biology

authors： Nakazawa Y,Hiraguri A,Moriyama H,Fukuhara T

更新日期：2007-04-01 00:00:00

abstract：:Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...

journal_title：Plant molecular biology

authors： Bastianelli F,Costa A,Vescovi M,D'Apuzzo E,Zottini M,Chiurazzi M,Lo Schiavo F

更新日期：2010-03-01 00:00:00

abstract：:Acyl-CoA binding proteins (ACBPs) are small (ca. 10 kDa) highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A novel cDNA encoding ACBP1, a predicted membrane protein of 24.1 kDa with an acyl-CoA binding protein domain at its carboxy terminus, was cloned from Arabidopsis thaliana. At this domain, ACBP1...

journal_title：Plant molecular biology

authors： Chye ML

更新日期：1998-11-01 00:00:00

abstract：:We report the successful combination of mRNA differential-display reverse-transcription PCR (DDRT-PCR) and 5'-rapid amplification of cDNA ends (5'-RACE) in order to isolate full-length cDNAs corresponding to genes activated in tobacco cells treated with cryptogein within 60 min. Cloning and sequencing of two cDNAs, ca...

journal_title：Plant molecular biology

authors： Petitot AS,Blein JP,Pugin A,Suty L

更新日期：1997-10-01 00:00:00

abstract：:In bacterial and animal coenzyme A (CoA) biosynthesis, pantothenate kinase (PANK) activity is critical in regulating intracellular CoA levels. Less is known about the role of PANK in plants, although a single plant isozyme from Arabidopsis, AtPANK1, was previously cloned and analyzed in vitro. We report here the chara...

journal_title：Plant molecular biology

authors： Tilton GB,Wedemeyer WJ,Browse J,Ohlrogge J

更新日期：2006-07-01 00:00:00

abstract：:Gibberellins (GAs) are important plant growth regulators, regulating many plant developmental processes, including seed germination, root and stem elongation, rosette expansion, floral induction and anther development. The diverse effects of GAs on plant development make it critical to maintain an appropriate endogeno...

journal_title：Plant molecular biology

authors： Zhang Y,Schwarz S,Saedler H,Huijser P

更新日期：2007-02-01 00:00:00

abstract：:Sugar is an important resource for energy generation and developmental regulation in plants, and sucrose starvation causes enormous changes in cellular morphology, enzyme activities and gene expression. Genome-wide gene expression profiling provides a comprehensive knowledge of gene expression under nutrient depletion...

journal_title：Plant molecular biology

authors： Wang HJ,Wan AR,Hsu CM,Lee KW,Yu SM,Jauh GY

更新日期：2007-03-01 00:00:00

abstract：:Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...

journal_title：Plant molecular biology

authors： Katinakis P,Lankhorst RM,Louwerse J,van Kammen A,van den Bos RC

更新日期：1988-03-01 00:00:00