Rejuvenation by shoot apex culture recapitulates the developmental increase of methylation at the maize gene Pl-Blotched.

abstract：:To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-NPTII reporter gene in a T-DNA construct. A number of transgenic N. sy...

journal_title：Plant molecular biology

authors： Kunz C,Narangajavana J,Jakowitsch J,Park YD,Delon TR,Kovarik A,Koukalová B,van der Winden J,Moscone E,Aufsatz W,Mette MF,Matzke M,Matzke AJ

更新日期：2003-05-01 00:00:00

abstract：:The chromatin structure of pea (Pisum sativum) rbcS genes in inactive (root), potentially active (dark-grown leaf), and active states (light-grown leaf) was analysed using (a) pancreatic DNAseI to detect general DNAseI sensitivity and DNAseI-hypersensitive sites, and (b) methyl-sensitive restriction endonucleases to p...

journal_title：Plant molecular biology

authors： Görz A,Schäfer W,Hirasawa E,Kahl G

更新日期：1988-09-01 00:00:00

abstract：:Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...

journal_title：Plant molecular biology

authors： Lai SJ,Lai MC,Lee RJ,Chen YH,Yen HE

更新日期：2014-07-01 00:00:00

abstract：KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...

journal_title：Plant molecular biology

authors： Zhang L,Wang Y,Zhang Q,Jiang Y,Zhang H,Li R

更新日期：2020-01-01 00:00:00

abstract：:To analyse experimentally the correlation between transgene silencing and the presence of an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the beta-glucuronidase (gus) gene was studied when present as a convergently transcribed inverted repeat or as a single copy in otherwise isogenic lines....

journal_title：Plant molecular biology

authors： De Buck S,Van Montagu M,Depicker A

更新日期：2001-07-01 00:00:00

abstract：:Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...

journal_title：Plant molecular biology

authors： Palauqui JC,Vaucheret H

更新日期：1995-10-01 00:00:00

abstract：:The genetics of male fertility restoration and the RFLP of mitochondrial DNA were studied for 16 sunflower cytoplasms (15 male-sterile and a male-fertile). Male fertility restoration/male sterility maintenance patterns distinguished 12 cytotypes. Four cytoplasms were completely unrestored so they were not distinguishe...

journal_title：Plant molecular biology

authors： Crouzillat D,de la Canal L,Perrault A,Ledoigt G,Vear F,Serieys H

更新日期：1991-03-01 00:00:00

abstract：:Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleas...

journal_title：Plant molecular biology

authors： Mahfouz MM,Li L,Piatek M,Fang X,Mansour H,Bangarusamy DK,Zhu JK

更新日期：2012-02-01 00:00:00

abstract：:In this report, we describe the efficient expression and glycosylation, in insect cells, of β-phaseolin polypeptides (M r 45 and 48 kDa) from Phaseolus vulgaris, by means of a baculovirus expression vector. N-terminal sequence analysis demonstrated that the signal peptide was efficiently processed. Tunicamycin treatme...

journal_title：Plant molecular biology

authors： Bustos MM,Luckow VA,Griffing LR,Summers MD,Hall TC

更新日期：1988-11-01 00:00:00

abstract：:A highly purified RNA polymerase preparation from pea chloroplasts has been shown to specifically transcribe the 16S rRNA gene in vitro using the recombinant pCB2-8 DNA as a template. The RNA polymerase has been found to show maximum activity and specificity with pea supercoiled rDNA as a template. At low concentratio...

journal_title：Plant molecular biology

authors： Sun E,Shapiro DR,Wu BW,Tewari KK

更新日期：1986-11-01 00:00:00

abstract：KEY MESSAGE:OsWRKY36 represses plant height and grain size by inhibiting gibberellin signaling. Plant height and grain size are important agronomic traits affecting yield in cereals, including rice. Gibberellins (GAs) are plant hormones that promote plant growth and developmental processions such as stem elongation and...

journal_title：Plant molecular biology

authors： Lan J,Lin Q,Zhou C,Ren Y,Liu X,Miao R,Jing R,Mou C,Nguyen T,Zhu X,Wang Q,Zhang X,Guo X,Liu S,Jiang L,Wan J

更新日期：2020-11-01 00:00:00

abstract：:We have previously isolated a Brassica juncea cDNA encoding BjCHI1, a novel chitinase with two chitin-binding domains, and have shown that its mRNA is induced by wounding and methyl jasmonate treatment (K.-J. Zhao and M.-L. Chye, Plant Mol. Biol. 40 (1999) 1009-1018). By the presence of two chitin-binding domains, BjC...

journal_title：Plant molecular biology

authors： Fung KL,Zhao KJ,He ZM,Chye ML

更新日期：2002-09-01 00:00:00

abstract：:WRKY transcription factors are involved in various biological processes, such as development, metabolism and responses to stress. However, their exact roles in abiotic stress tolerance are largely unknown. Here, we demonstrated a working model for the function of a WRKY gene (ThWRKY4) from Tamarix hispida in the stres...

journal_title：Plant molecular biology

authors： Zheng L,Liu G,Meng X,Liu Y,Ji X,Li Y,Nie X,Wang Y

更新日期：2013-07-01 00:00:00

abstract：:Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...

journal_title：Plant molecular biology

authors： Ni Z,Hu Z,Jiang Q,Zhang H

更新日期：2013-05-01 00:00:00

abstract：:A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...

journal_title：Plant molecular biology

authors： Man AL,Purcell PC,Hannappel U,Halford NG

更新日期：1997-05-01 00:00:00

abstract：:The treatment of barley aleurone layers with gibberellic acid (GA3) results in the synthesis of two groups of α-amylase isozymes. Addition of abscisic acid (ABA) at the same time as GA3 inhibited the synthesis of both groups of isozymes. However, midcourse ABA addition (12 h or later after GA3) had a more inhibitory e...

journal_title：Plant molecular biology

authors： Nolan RC,Lin LS,Ho TH

更新日期：1987-01-01 00:00:00

abstract：:We have sequenced and characterized the complete psbB gene cluster of Chlamydomonas reinhardtii chloroplast DNA. Although the petB and petD genes are located elsewhere, the sequential order of psbB, ORF31, psbN and psbH is identical to that of the psbB operon in higher plants. Also, intergenic non-coding regions are m...

journal_title：Plant molecular biology

authors： Johnson CH,Schmidt GW

更新日期：1993-07-01 00:00:00

abstract：:Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immunohistochemical analysis....

journal_title：Plant molecular biology

authors： Benvenuto E,Ordàs RJ,Tavazza R,Ancora G,Biocca S,Cattaneo A,Galeffi P

更新日期：1991-10-01 00:00:00

abstract：:Alternatively spliced introns are the ones that are usually spliced but can be occasionally retained in a transcript isoform. They are the most frequently used alternative splice form in plants (~50% of alternative splicing events). Chlamydomonas reinhardtii, a unicellular alga, is a good model to understand alternati...

journal_title：Plant molecular biology

authors： Raj-Kumar PK,Vallon O,Liang C

更新日期：2017-06-01 00:00:00

abstract：:The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...

journal_title：Plant molecular biology

authors： Abbott AG,O'Dell M,Flavell RB

更新日期：1985-07-01 00:00:00

abstract：:Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Phytohormones such as abscisic acid (ABA) and indole-3-acetic acid (IAA) play critical roles in developmental progresses and environmental responses through complex signalling networks. However, crosstalk between the tw...

journal_title：Plant molecular biology

authors： Du H,Wu N,Chang Y,Li X,Xiao J,Xiong L

更新日期：2013-11-01 00:00:00

abstract：:Soil contamination by hexavalent chromium [Cr(VI) or chromate] due to anthropogenic activities has become an increasingly important environmental problem. To date few studies have been performed to elucidate the signaling networks involved on adaptive responses to (CrVI) toxicity in plants. In this work, we report tha...

journal_title：Plant molecular biology

authors： Martínez-Trujillo M,Méndez-Bravo A,Ortiz-Castro R,Hernández-Madrigal F,Ibarra-Laclette E,Ruiz-Herrera LF,Long TA,Cervantes C,Herrera-Estrella L,López-Bucio J

更新日期：2014-09-01 00:00:00

abstract：:Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic ...

journal_title：Plant molecular biology

authors： Packer JC,André D,Howe CJ

更新日期：1995-01-01 00:00:00

abstract：:The expression of the mat-r locus (mat-r-nad1e-nad5III) was studied in wheat mitochondria. Transcription initiation sites were mapped by S1 protection, primer extension and capping experiments. Two different transcription initiation sites were found. One, non-canonical promoter of low expression level generates a tran...

journal_title：Plant molecular biology

authors： Farré JC,Araya A

更新日期：1999-08-01 00:00:00

abstract：:To isolate differentially expressed genes during the juvenile-to-adult phase transition of an early-flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata), suppression subtractive hybridization was performed. In total, 463 cDNA clones chosen by differential screening of 1,920 clones wer...

journal_title：Plant molecular biology

authors： Zhang JZ,Ai XY,Sun LM,Zhang DL,Guo WW,Deng XX,Hu CG

更新日期：2011-05-01 00:00:00

abstract：:The small GTPases of the Rho family play a key role in actin cytoskeletal organization. In plants, a novel Rho subfamily, called ROP (Rho of plants), has been found. In Arabidopsis, 12 ROP GTPases have been identified which differ mainly at their C-termini. To test the localization of two members of this subfamily (At...

journal_title：Plant molecular biology

authors： Bischoff F,Vahlkamp L,Molendijk A,Palme K

更新日期：2000-02-01 00:00:00

abstract：:A novel CBL-interacting protein kinase (CIPK) gene, ZmCIPK16, was isolated from maize (Zea mays), which has been certified to have two copies in the genome. The ZmCIPK16 is strongly induced in maize seedlings by PEG, NaCl, ABA, dehydration, heat and drought, but not by cold. A yeast two-hybrid assay demonstrated that ...

journal_title：Plant molecular biology

authors： Zhao J,Sun Z,Zheng J,Guo X,Dong Z,Huai J,Gou M,He J,Jin Y,Wang J,Wang G

更新日期：2009-04-01 00:00:00

abstract：:The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleoti...

journal_title：Plant molecular biology

authors： Rasmussen OF,Bookjans G,Stummann BM,Henningsen KW

更新日期：1984-07-01 00:00:00

abstract：:TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...

journal_title：Plant molecular biology

authors： Miao ZH,Liu X,Lam E

更新日期：1994-04-01 00:00:00

abstract：:The stress-induced expression of four different ubiquitin-encoding cDNAs was characterized in potato tuber tissue. The four clones exhibited differences in both structure and expression. The first cDNA encoded a single ubiquitin unit fused to an 80 amino acid ribosomal extension protein identical to the extension prot...

journal_title：Plant molecular biology

authors： Garbarino JE,Rockhold DR,Belknap WR

更新日期：1992-10-01 00:00:00