Localization of AtROP4 and AtROP6 and interaction with the guanine nucleotide dissociation inhibitor AtRhoGDI1 from Arabidopsis.

Abstract:

:The small GTPases of the Rho family play a key role in actin cytoskeletal organization. In plants, a novel Rho subfamily, called ROP (Rho of plants), has been found. In Arabidopsis, 12 ROP GTPases have been identified which differ mainly at their C-termini. To test the localization of two members of this subfamily (AtROP4 and AtROP6), we have generated translational fusions with the green fluorescent protein (GFP). Microscopic analysis of transiently transfected BY2 cells revealed a predominant localization of AtROP4 in the perinuclear region, while AtROP6 was localized almost exclusively to the plasma membrane. Swapping of the AtROP4 and AtROP6 C-termini produced a change in localization. As RhoGDIs are known to bind to the C-terminus of GTPases of the Rho family, we searched for Arabidopsis RhoGDI genes. We identified the AtRhoGDI1 gene and mapped it to chromosome 3. AtRhoGDI1 encodes a 22.5 kDa protein which contains highly conserved amino acids in the isoprene binding pocket and exhibits 29% to 37% similarity to known mammalian RhoGDI homologues. The AtRhoGDI1 gene was expressed in all tissues studied. Using the yeast two-hybrid system, we showed specific interaction of AtRhoGDI1 with both AtROP4 and AtROP6 as well as with their GTP-locked mutants, but not with a GTPase of the RAB family. Recombinant GST-AtRhoGDI1 could bind GFP-AtROP4 from transgenic tobacco BY2 cell extracts, confirming the interaction observed with the two-hybrid system.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Bischoff F,Vahlkamp L,Molendijk A,Palme K

doi

10.1023/a:1006341210147

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

515-30

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

42

pub_type

杂志文章
  • Understanding Darjeeling tea flavour on a molecular basis.

    abstract::Darjeeling teas are the highest grown teas in the world and preferred for its flavour, aroma and quality. Apart from the genetic makeup of the plant, earlier reports suggest that insect infestation, particularly jassids and thrips triggers the aroma and flavour formation in Darjeeling tea. The present work encompasses...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-012-9887-0

    authors: Gohain B,Borchetia S,Bhorali P,Agarwal N,Bhuyan LP,Rahman A,Sakata K,Mizutani M,Shimizu B,Gurusubramaniam G,Ravindranath R,Kalita MC,Hazarika M,Das S

    更新日期:2012-04-01 00:00:00

  • Oryza sativa heat-induced RING finger protein 1 (OsHIRP1) positively regulates plant response to heat stress.

    abstract:KEY MESSAGE:OsHIRP1 is an E3 ligase that acts as a positive regulator in the plant response to heat stress, thus providing important information relating to adaptation and regulation under heat stress in plant. Extreme temperature adversely affects plant growth, development, and productivity. Here, we report the molecu...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00835-9

    authors: Kim JH,Lim SD,Jang CS

    更新日期:2019-04-01 00:00:00

  • Chloroplast localization of methylerythritol 4-phosphate pathway enzymes and regulation of mitochondrial genes in ispD and ispE albino mutants in Arabidopsis.

    abstract::Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9297-5

    authors: Hsieh MH,Chang CY,Hsu SJ,Chen JJ

    更新日期:2008-04-01 00:00:00

  • Synthesis versus degradation: directions of amino acid metabolism during Arabidopsis abiotic stress response.

    abstract:KEY MESSAGE:During abiotic stress low abundant amino acids are not synthesized but they accumulate due to increased protein turnover under conditions inducing carbohydrate starvation (dehydration, salt stress, darkness) and are degraded. Metabolic adaptation is crucial for abiotic stress resistance in plants, and accum...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-018-0767-0

    authors: Hildebrandt TM

    更新日期:2018-09-01 00:00:00

  • Simple binary vectors for DNA transfer to plant cells.

    abstract::Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00015678

    authors: van den Elzen P,Lee KY,Townsend J,Bedbrook J

    更新日期:1985-05-01 00:00:00

  • A novel protein elicitor (SsCut) from Sclerotinia sclerotiorum induces multiple defense responses in plants.

    abstract::In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco th...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-014-0244-3

    authors: Zhang H,Wu Q,Cao S,Zhao T,Chen L,Zhuang P,Zhou X,Gao Z

    更新日期:2014-11-01 00:00:00

  • Tobacco microtubule-associated protein, MAP65-1c, bundles and stabilizes microtubules.

    abstract::Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9694-4

    authors: Meng Q,Du J,Li J,Lü X,Zeng X,Yuan M,Mao T

    更新日期:2010-12-01 00:00:00

  • Lignin synthesized by CmCAD2 and CmCAD3 in oriental melon (Cucumis melo L.) seedlings contributes to drought tolerance.

    abstract:KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01018-7

    authors: Liu W,Jiang Y,Wang C,Zhao L,Jin Y,Xing Q,Li M,Lv T,Qi H

    更新日期:2020-08-01 00:00:00

  • Rapid one-step protein purification from plant material using the eight-amino acid StrepII epitope.

    abstract::Beyond the rewards of plant genome analysis and gene identification, characterisation of protein activities, post-translational modifications and protein complex composition remains a challenge for plant biologists. Ideally, methods should allow rapid isolation of proteins from plant material achieving a high degree o...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-0501-y

    authors: Witte CP,Noël LD,Gielbert J,Parker JE,Romeis T

    更新日期:2004-05-01 00:00:00

  • Regulation of chalcone flavanone isomerase (CHI) gene expression inPetunia hybrida: the use of alternative promoters in corolla, anthers and pollen.

    abstract::In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00036968

    authors: van Tunen AJ,Hartman SA,Mur LA,Mol JN

    更新日期:1989-05-01 00:00:00

  • Unusual sequence of an abscisic acid-inducible mRNA which accumulates late in Brassica napus seed development.

    abstract::We have analyzed the nucleotide sequence and accumulation of an mRNA which is prevalent in seeds of Brassica napus L. During normal development, the mRNA begins to accumulate during late embryogeny, is stored in dry seeds, and becomes undetectable in seedlings within 24 hours after imbibition. Moreover, abscisic acid ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017579

    authors: Harada JJ,Delisle AJ,Baden CS,Crouch ML

    更新日期:1989-04-01 00:00:00

  • Isolation of a cDNA encoding an Arabidopsis galactokinase by functional expression in yeast.

    abstract::A cDNA clone encoding Arabidopsis thaliana galactokinase was fortuitously isolated during the course of a screen for plant homologues of a Saccharomyces cerevisiae peroxisome assembly gene, PAS9. Clones were sought which restored the ability of pas9 cells to grow on oleate as a sole carbon source, as oleate metabolism...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005816104743

    authors: Kaplan CP,Tugal HB,Baker A

    更新日期:1997-06-01 00:00:00

  • Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum.

    abstract::Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019191

    authors: Packer JC,André D,Howe CJ

    更新日期:1995-01-01 00:00:00

  • Long-range physical maps of two loci (Aps-1 and GP79) flanking the root-knot nematode resistance gene (Mi) near the centromere of tomato chromosome 6.

    abstract::The root knot nematode resistance gene Mi in tomato has been mapped in the pericentromeric region of chromosome 6. With the objective of isolating Mi through a map-based cloning approach, we have previously identified and ordered into a high-resolution genetic linkage map a variety of tightly linked molecular markers....

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021430

    authors: van Daelen RA,Gerbens F,van Ruissen F,Aarts J,Hontelez J,Zabel P

    更新日期:1993-10-01 00:00:00

  • Phenotypic changes in Arabidopsis caused by expression of a yeast vacuolar Ca2+/H+ antiporter.

    abstract::In plants, cytosolic Ca2+ levels are tightly regulated, and changes in cytosolic Ca2+ have been implicated in converting numerous signals into adapted responses. Vacuolar ion transporters are thought to be key mediators of cytosolic Ca2+ concentrations. In an attempt to interpret the role of vacuolar Ca2+ transport in...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1010620227913

    authors: Hirschi KD,Miranda ML,Wilganowski NL

    更新日期:2001-05-01 00:00:00

  • Gene regulation in planta by plant-derived engineered zinc finger protein transcription factors.

    abstract::The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-7820-x

    authors: Holmes-Davis R,Li G,Jamieson AC,Rebar EJ,Liu Q,Kong Y,Case CC,Gregory PD

    更新日期:2005-02-01 00:00:00

  • CaaX-prenyltransferases are essential for expression of genes involvedin the early stages of monoterpenoid biosynthetic pathway in Catharanthus roseus cells.

    abstract::CaaX-prenyltransferases (CaaX-PTases) catalyse the covalent attachment of isoprenyl groups to conserved cysteine residues located at the C-terminal CaaX motif of a protein substrate. This post-translational modification is required for the function and/or subcellular localization of some transcription factors and comp...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-3095-0

    authors: Courdavault V,Thiersault M,Courtois M,Gantet P,Oudin A,Doireau P,St-Pierre B,Giglioli-Guivarc'h N

    更新日期:2005-04-01 00:00:00

  • High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda).

    abstract::Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultiva...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006126609534

    authors: Wenck AR,Quinn M,Whetten RW,Pullman G,Sederoff R

    更新日期:1999-02-01 00:00:00

  • The ltk gene family encodes novel receptor-like kinases with temporal expression in developing maize endosperm.

    abstract::We describe the isolation and characterization of maize cDNAs that are transcribed from a small gene family and encode a novel group of receptor-like kinases (RLKs). The distinctive extracellular domain of these novel RLKs includes a unique number and arrangement of leucine-rich repeats (LRRs), a proline-rich region (...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006012530241

    authors: Li Z,Wurtzel ET

    更新日期:1998-07-01 00:00:00

  • The effect of abscisic acid on the differential expression of α-amylase isozymes in barley aleurone layers.

    abstract::The treatment of barley aleurone layers with gibberellic acid (GA3) results in the synthesis of two groups of α-amylase isozymes. Addition of abscisic acid (ABA) at the same time as GA3 inhibited the synthesis of both groups of isozymes. However, midcourse ABA addition (12 h or later after GA3) had a more inhibitory e...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00016430

    authors: Nolan RC,Lin LS,Ho TH

    更新日期:1987-01-01 00:00:00

  • Tobacco plants transformed with cdc25, a mitotic inducer gene from fission yeast.

    abstract::We investigated the effects of expressing a cDNA of cdc25, a mitotic inducer gene of Schizosaccharomyces pombe, on the development of transgenic tobacco plants (Nicotiana tabacum cv. Samsun). Nine independent primary transformants were regenerated containing the cdc25 sequence under the control of a cauliflower mosaic...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019293

    authors: Bell MH,Halford NG,Ormrod JC,Francis D

    更新日期:1993-11-01 00:00:00

  • Molecular cloning and nucleotide sequence cDNA encoding nucleoside diphosphate kinase of rice (Oryza sativa L.).

    abstract::We isolated a rice cDNA encoding nucleoside diphosphate kinase (NDK, EC 2.7.4.6). The deduced amino acid sequence of the rice NDK shows highest homology to spinach NDK-I. The rice NDK gene exhibits a strong codon bias (73.8% GC) in the third position of the codon. DNA blot analysis indicated that at least single NDK g...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021824

    authors: Yano A,Shimazaki T,Kato A,Umeda M,Uchimiya H

    更新日期:1993-12-01 00:00:00

  • Expression of nodule-specific genes in Phaseolus vulgaris L.

    abstract::The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020530

    authors: Campos F,Padilla J,Vázquez M,Ortega JL,Enríquez C,Sánchez F

    更新日期:1987-11-01 00:00:00

  • Non-photosynthetic 'malic enzyme' from maize: a constituvely expressed enzyme that responds to plant defence inducers.

    abstract::The characterization of a non-photosynthetic isoform of NADP-malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1010665910095

    authors: Maurino VG,Saigo M,Andreo CS,Drincovich MF

    更新日期:2001-03-01 00:00:00

  • Agrobacterium-mediated sorghum transformation.

    abstract::Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a 'Super-binary' vector with a bar gene as a selectable marker for herbicide resista...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1026507517182

    authors: Zhao ZY,Cai T,Tagliani L,Miller M,Wang N,Pang H,Rudert M,Schroeder S,Hondred D,Seltzer J,Pierce D

    更新日期:2000-12-01 00:00:00

  • Context sequences of translation initiation codon in plants.

    abstract::In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/a:1005816823636

    authors: Joshi CP,Zhou H,Huang X,Chiang VL

    更新日期:1997-12-01 00:00:00

  • Mutational analysis of the conserved domains of a T-region border repeat of Agrobacterium tumefaciens.

    abstract::Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine an...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027312

    authors: van Haaren MJ,Sedee NJ,de Boer HA,Schilperoort RA,Hooykaas PJ

    更新日期:1989-11-01 00:00:00

  • Computational prediction and experimental verification of HVA1-like abscisic acid responsive promoters in rice (Oryza sativa).

    abstract::Abscisic acid (ABA) is one of the central plant hormones, responsible for controlling both maturation and germination in seeds, as well as mediating adaptive responses to desiccation, injury, and pathogen infection in vegetative tissues. Thorough analyses of two barley genes, HVA1 and HVA22, indicate that their respon...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9017-y

    authors: Ross C,Shen QJ

    更新日期:2006-09-01 00:00:00

  • Complete sequence of the mitochondrial DNA of Chlamydomonas eugametos.

    abstract::The complete nucleotide sequence of the Chlamydomonas eugametos (Chlamydomonadales, Chlorophyceae, sensu Mattox and Stewart) mitochondrial genome has been determined (22,897 bp, 34.6% G + C). The genes identified in this circular-mapping genome include those for apocytochrome b, subunit 1 of the cytochrome oxidase com...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005995718091

    authors: Denovan-Wright EM,Nedelcu AM,Lee RW

    更新日期:1998-01-01 00:00:00

  • The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds.

    abstract::A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9296-6

    authors: Portnoy V,Benyamini Y,Bar E,Harel-Beja R,Gepstein S,Giovannoni JJ,Schaffer AA,Burger J,Tadmor Y,Lewinsohn E,Katzir N

    更新日期:2008-04-01 00:00:00